BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,472,897	A→T	47.9%	T73S (ACT→TCT)	dsdA →	D‑serine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,472,897	0	A	T	47.9%	40.2 / inf	278	T73S (ACT→TCT)	dsdA	D‑serine dehydratase
Reads supporting (aligned to +/- strand): ref base A (64/80); new base T (59/74); total (124/154)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 280 total aligned reads displayed.

AAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGG  >  CP009273/2472748‑2473046

 aGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCAc                                                                                                                                                      >  2:3685710/1‑150 (MQ=255)

   aaaCCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTg                                                                                                                                                    <  2:1493996/150‑1 (MQ=255)

   aaaCCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTg                                                                                                                                                    <  1:2941863/150‑1 (MQ=255)

        accTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTgggggg                                                                                                                                               <  1:1484844/150‑1 (MQ=255)

          cTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGat                                                                                                                                             >  2:3957386/1‑150 (MQ=255)

             gTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGattat                                                                                                                                          >  2:1067030/1‑150 (MQ=255)

             gTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGattat                                                                                                                                          <  1:2770193/150‑1 (MQ=255)

              tttAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGattatt                                                                                                                                         >  2:3829324/1‑150 (MQ=255)

              tttAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGattatt                                                                                                                                         >  1:3126569/1‑150 (MQ=255)

                tAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGC‑AAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGaa                                                                                                                                      >  2:1144450/1‑150 (MQ=255)

                 aaTCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGaa                                                                                                                                      <  2:1321063/150‑1 (MQ=255)

                    ccTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCa                                                                                                                                   >  1:527786/1‑150 (MQ=255)

                    ccTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCa                                                                                                                                   >  1:2650662/1‑150 (MQ=255)

                     cTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAg                                                                                                                                  <  1:3817424/150‑1 (MQ=255)

                      tGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGa                                                                                                                                 <  2:4170698/150‑1 (MQ=255)

                      tGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGa                                                                                                                                 >  2:483420/1‑150 (MQ=255)

                         cACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACt                                                                                                                              <  2:2368664/150‑1 (MQ=255)

                            gACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGt                                                                                                                           <  1:2885713/150‑1 (MQ=255)

                              ccTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAAGTGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTg                                                                                                                         <  1:489061/150‑1 (MQ=255)

                                  aTTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCAt                                                                                                                     >  2:860794/1‑150 (MQ=255)

                                  aTTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCAt                                                                                                                     <  2:4027808/150‑1 (MQ=255)

                                      gCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCa                                                                                                                 <  1:368486/150‑1 (MQ=255)

                                      gCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCa                                                                                                                 <  2:4111274/150‑1 (MQ=255)

                                      gCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCa                                                                                                                 <  1:1657680/150‑1 (MQ=255)

                                         gAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCt                                                                                                              >  1:3735766/1‑150 (MQ=255)

                                          aaGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTa                                                                                                             >  2:2012259/1‑150 (MQ=255)

                                          aaGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTa                                                                                                             <  2:684205/150‑1 (MQ=255)

                                            ggTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATg                                                                                                           <  1:142339/150‑1 (MQ=255)

                                              tttACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCa                                                                                                         >  1:3224470/1‑150 (MQ=255)

                                               ttACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCaa                                                                                                        >  2:3624971/1‑150 (MQ=255)

                                                 aCCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCaaaa                                                                                                      >  1:1461826/1‑150 (MQ=255)

                                                 aCCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCaaaa                                                                                                      <  2:3613877/150‑1 (MQ=255)

                                                    ttATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACg                                                                                                   <  2:1286649/150‑1 (MQ=255)

                                                     tATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACgg                                                                                                  <  1:3069042/150‑1 (MQ=255)

                                                        gTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTg                                                                                               <  2:2070546/150‑1 (MQ=255)

                                                         ttGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTgg                                                                                              <  2:583388/150‑1 (MQ=255)

                                                         ttGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTgg                                                                                              >  1:1912645/1‑150 (MQ=255)

                                                         ttGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTgg                                                                                              >  1:2599935/1‑150 (MQ=255)

                                                          tGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGa                                                                                             <  1:2579213/150‑1 (MQ=255)

                                                           ggCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGaa                                                                                            >  2:1674170/1‑150 (MQ=255)

                                                               tGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAg                                                                                        <  1:658037/150‑1 (MQ=255)

                                                               tGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAg                                                                                        >  1:714527/1‑150 (MQ=255)

                                                                     aaCAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATc                                                                                  <  2:3880060/150‑1 (MQ=255)

                                                                       cAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACTGCTGGAAAAAGAATATCAg                                                                                >  2:745172/1‑150 (MQ=255)

                                                                        aGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGc                                                                               <  2:2845527/150‑1 (MQ=255)

                                                                        aGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGc                                                                               >  1:2856962/1‑150 (MQ=255)

                                                                             gTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCg                                                                          <  1:2202781/150‑1 (MQ=255)

                                                                             gTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCg                                                                          <  1:1517511/150‑1 (MQ=255)

                                                                                 aGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCa                                                                      >  2:2987993/1‑150 (MQ=255)

                                                                                 aGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCa                                                                      <  2:2525978/150‑1 (MQ=255)

                                                                                  ggACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGACTCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAg                                                                     >  2:189069/1‑150 (MQ=255)

                                                                                    aCGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCg                                                                   <  2:901175/150‑1 (MQ=255)

                                                                                     cGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCgg                                                                  >  2:2888351/1‑150 (MQ=255)

                                                                                       cccATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGc                                                                >  2:3412212/1‑150 (MQ=255)

                                                                                        ccATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCa                                                               >  2:4145316/1‑150 (MQ=255)

                                                                                        ccATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCa                                                               >  1:2353148/1‑150 (MQ=255)

                                                                                         cATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCaa                                                              <  1:449383/150‑1 (MQ=255)

                                                                                          aTGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAAc                                                             >  1:3902956/1‑150 (MQ=255)

                                                                                          aTGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAAc                                                             <  1:3478772/150‑1 (MQ=255)

                                                                                            gcgcgcTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTg                                                           <  2:3600440/150‑1 (MQ=255)

                                                                                              gcgcTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGtt                                                         <  2:2254372/150‑1 (MQ=255)

                                                                                                gcTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTAc                                                       >  2:2482680/1‑150 (MQ=255)

                                                                                                  ttATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTg                                                     <  2:825628/150‑1 (MQ=255)

                                                                                                     tCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGaaa                                                  <  1:3618405/150‑1 (MQ=255)

                                                                                                      cccGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGaaaa                                                 <  1:2050315/150‑1 (MQ=255)

                                                                                                          ttttGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGa                                             >  1:4141784/1‑150 (MQ=255)

                                                                                                           tttGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGAt                                            <  1:2984843/150‑1 (MQ=255)

                                                                                                            ttGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATa                                           >  1:113014/1‑150 (MQ=255)

                                                                                                             tGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAg                                          <  2:1549340/150‑1 (MQ=255)

                                                                                                              gCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGc                                         >  1:108138/1‑150 (MQ=255)

                                                                                                              gCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGc                                         >  1:3380184/1‑150 (MQ=255)

                                                                                                                aCCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATGGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAAACGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCa                                       >  2:450529/1‑150 (MQ=255)

                                                                                                                 cccTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCAt                                      >  1:1058125/1‑150 (MQ=255)

                                                                                                                 cccTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCAt                                      >  2:1645070/1‑150 (MQ=255)

                                                                                                                   cTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCAttt                                    >  2:2313937/1‑150 (MQ=255)

                                                                                                                   cTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCAttt                                    <  2:3933101/150‑1 (MQ=255)

                                                                                                                   cTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCAttt                                    >  1:3308063/1‑150 (MQ=255)

                                                                                                                   cTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCAttt                                    <  2:239486/150‑1 (MQ=255)

                                                                                                                    tATCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTg                                   >  1:1582835/1‑150 (MQ=255)

                                                                                                                     aTCTGGCAAAAGCATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGc                                  <  2:1582913/150‑1 (MQ=255)

                                                                                                                       cTGGCAAAAGCATTTTCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGccc                                <  1:3905756/150‑1 (MQ=255)

                                                                                                                         ggCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCAt                              >  2:213254/1‑150 (MQ=255)

                                                                                                                             aaaGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTcc                          <  1:1210031/150‑1 (MQ=255)

                                                                                                                              aaGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCg                         >  1:3048614/1‑150 (MQ=255)

                                                                                                                              aaGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCg                         <  1:574777/150‑1 (MQ=255)

                                                                                                                               aGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCgg                        <  1:1666204/150‑1 (MQ=255)

                                                                                                                               aGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCgg                        >  1:3254198/1‑150 (MQ=255)

                                                                                                                                 cATTTCCTGAAACTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCt                      <  1:2996152/150‑1 (MQ=255)

                                                                                                                                 cATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCt                      >  2:3074288/1‑150 (MQ=255)

                                                                                                                                    ttCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCa                   <  1:1613275/150‑1 (MQ=255)

                                                                                                                                      ccTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATa                 >  1:1302429/1‑150 (MQ=255)

                                                                                                                                           aaCTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGc            >  1:1353096/1‑150 (MQ=255)

                                                                                                                                           aaCTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGc            >  1:929468/1‑150 (MQ=255)

                                                                                                                                            aCTGCTGCCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCa           <  2:2924721/150‑1 (MQ=255)

                                                                                                                                            aCTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCa           >  2:2491392/1‑150 (MQ=255)

                                                                                                                                             ctgctgCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCAc          <  1:4006587/150‑1 (MQ=255)

                                                                                                                                              tgctgcCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCAcg         >  2:3266224/1‑150 (MQ=255)

                                                                                                                                               gctgcCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCAcgc        <  1:321333/150‑1 (MQ=255)

                                                                                                                                                ctgcCTCTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCAcgcg       <  2:2622788/150‑1 (MQ=255)

                                                                                                                                                  gcCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACgcggc     <  2:3181995/150‑1 (MQ=255)

AAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATGCAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGG  >  CP009273/2472748‑2473046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`