Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,561,221 C→A 52.0% P82P (CCG→CCT)  eutH ← ethanolamine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,561,2210CA52.0% 56.9 / inf 221P82P (CCG→CCT) eutHethanolamine transporter
Reads supporting (aligned to +/- strand):  ref base C (41/65);  new base A (56/59);  total (97/124)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 224 total aligned reads displayed.

CCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATACTTTTACCGAACTTACCGAGGA  >  CP009273/2561072‑2561369
                                                                                                                                                     |                                                                                                                                                    
  catcatCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCgg                                                                                                                                                    >  1:1419679/1‑150 (MQ=255)
  catcatCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAgg                                                                                                                                                    >  2:2283808/1‑150 (MQ=255)
   atcatcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGa                                                                                                                                                   <  1:2719515/150‑1 (MQ=255)
     catcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat                                                                                                                                                 <  1:184986/150‑1 (MQ=255)
      atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaata                                                                                                                                                <  2:1506322/150‑1 (MQ=255)
        cGACCCGAGAATTATCCCAGAGTATATCCACGCGGCTACGTCGCCTCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTATCGCCGAGCATTTCGTTAACAGGaataat                                                                                                                                              >  2:1929266/1‑150 (MQ=255)
         gACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt                                                                                                                                             <  2:3784411/150‑1 (MQ=255)
         gACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt                                                                                                                                             <  1:441235/150‑1 (MQ=255)
         gACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAAtt                                                                                                                                             <  2:1315031/150‑1 (MQ=255)
          aCCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTa                                                                                                                                            >  2:1940220/1‑150 (MQ=255)
               agaATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGc                                                                                                                                       <  1:4030255/150‑1 (MQ=255)
               agaATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGc                                                                                                                                       <  1:974624/150‑1 (MQ=255)
                gaATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGcc                                                                                                                                      <  2:515964/150‑1 (MQ=255)
                 aaTTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGccc                                                                                                                                     <  1:2993615/150‑1 (MQ=255)
                   ttAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCga                                                                                                                                   <  2:1451906/150‑1 (MQ=255)
                    tAACCCCGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGATGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCgag                                                                                                                                  <  2:1082720/150‑1 (MQ=255)
                    tAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCgag                                                                                                                                  <  1:595347/150‑1 (MQ=255)
                       cccAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAAc                                                                                                                               <  2:2331543/150‑1 (MQ=255)
                        ccAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACg                                                                                                                              >  2:450425/1‑150 (MQ=255)
                          tgagTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTg                                                                                                                            <  2:3325455/149‑1 (MQ=255)
                             gTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGc                                                                                                                         >  1:210128/1‑150 (MQ=255)
                             gTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGc                                                                                                                         >  1:80292/1‑150 (MQ=255)
                             gTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTGTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGGTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAATCAGGAATAATTACCTGCCCGAGACCGTGAGc                                                                                                                         >  1:2591221/1‑150 (MQ=255)
                             gTAGAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGc                                                                                                                         <  2:1833315/150‑1 (MQ=255)
                              tataGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGACCATCGATGGGTTAGCGCCGAGCAGTTCGTAACCCGGAATAATTACCGGCCCGAGAACGTGAGcc                                                                                                                        >  2:1774911/1‑150 (MQ=255)
                              tataGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGcc                                                                                                                        <  1:1814610/150‑1 (MQ=255)
                               ataGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCa                                                                                                                       <  2:2810592/150‑1 (MQ=255)
                               ataGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCa                                                                                                                       >  2:916107/1‑150 (MQ=255)
                                 aGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGt                                                                                                                     <  1:168791/150‑1 (MQ=255)
                                  gCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTa                                                                                                                    <  1:2067900/150‑1 (MQ=255)
                                    cACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTAcc                                                                                                                  <  2:157743/150‑1 (MQ=255)
                                       gcgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGt                                                                                                               <  1:3730443/150‑1 (MQ=255)
                                        cgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTg                                                                                                              >  2:3431906/1‑150 (MQ=255)
                                         ggCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGc                                                                                                             >  1:3664790/1‑150 (MQ=255)
                                         ggCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGc                                                                                                             >  1:1313230/1‑150 (MQ=255)
                                         ggCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGc                                                                                                             >  1:1313471/1‑150 (MQ=255)
                                            tACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAg                                                                                                          <  2:3885044/150‑1 (MQ=255)
                                             aCGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAgc                                                                                                         >  1:1459787/1‑150 (MQ=255)
                                                    cgcccgccAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCa                                                                                                  >  2:2400928/1‑150 (MQ=255)
                                                     gcccgccAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCAt                                                                                                 >  1:1235928/1‑150 (MQ=255)
                                                        cgccAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATAcc                                                                                              >  2:693436/1‑150 (MQ=255)
                                                              ctctTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCAt                                                                                        <  1:2110088/150‑1 (MQ=255)
                                                                     gCGAGGAAGAAGCCGCCCATCTCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAgg                                                                                 <  1:3747160/150‑1 (MQ=255)
                                                                      cGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGc                                                                                >  2:1226463/1‑150 (MQ=255)
                                                                       gAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGcc                                                                               >  1:136309/1‑150 (MQ=255)
                                                                         ggAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCa                                                                             <  2:1797477/150‑1 (MQ=255)
                                                                          gaagaaGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAg                                                                            <  2:1885858/150‑1 (MQ=255)
                                                                          gaagaaGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATGACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAg                                                                            <  2:1230699/150‑1 (MQ=255)
                                                                           aagaagCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGt                                                                           >  2:2371275/1‑150 (MQ=255)
                                                                                   gccCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCAt                                                                   >  2:4008494/1‑150 (MQ=255)
                                                                                       atatCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGcc                                                               <  2:2221873/150‑1 (MQ=255)
                                                                                       atatCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGcc                                                               >  1:2196630/1‑150 (MQ=255)
                                                                                       atatCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGcc                                                               <  1:4095386/150‑1 (MQ=255)
                                                                                              aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGc                                                        >  2:949604/1‑150 (MQ=255)
                                                                                               cGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGcc                                                       <  1:3098597/150‑1 (MQ=255)
                                                                                                gCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCct                                                      <  1:3005602/150‑1 (MQ=255)
                                                                                                 ccAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCctt                                                     <  1:2495422/150‑1 (MQ=255)
                                                                                                  cagcagTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCcttc                                                    >  2:636644/1‑150 (MQ=255)
                                                                                                  cagcagTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCcttc                                                    >  1:91341/1‑150 (MQ=255)
                                                                                                    gcagTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATTCCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCcttctt                                                  <  2:1365986/150‑1 (MQ=255)
                                                                                                    gcagTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCcttctt                                                  <  1:1919686/150‑1 (MQ=255)
                                                                                                      agTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCg                                                >  2:1643552/1‑150 (MQ=255)
                                                                                                       gtgtTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGa                                               <  1:3326018/150‑1 (MQ=255)
                                                                                                         gtTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAAc                                             >  2:2549654/1‑150 (MQ=255)
                                                                                                         gtTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAAc                                             >  2:1831085/1‑150 (MQ=255)
                                                                                                          ttCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACt                                            >  2:4028393/1‑150 (MQ=255)
                                                                                                            ccGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGa                                          <  1:2024058/150‑1 (MQ=255)
                                                                                                            ccGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGa                                          <  2:4016911/150‑1 (MQ=255)
                                                                                                             cGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGAc                                         >  2:1249427/1‑150 (MQ=255)
                                                                                                              ggCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGAcc                                        <  2:3224321/150‑1 (MQ=255)
                                                                                                               gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCg                                       <  2:1259820/150‑1 (MQ=255)
                                                                                                                 gAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGcc                                     <  1:2791508/150‑1 (MQ=255)
                                                                                                                   aCATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTg                                   >  1:954346/1‑150 (MQ=255)
                                                                                                                    cATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGa                                  >  1:3190695/1‑150 (MQ=255)
                                                                                                                     aTCGATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGAt                                 <  2:2723315/150‑1 (MQ=255)
                                                                                                                      tCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACAGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATc                                >  2:3722481/1‑150 (MQ=255)
                                                                                                                       cGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATcc                               <  2:1800214/150‑1 (MQ=255)
                                                                                                                        gATGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCt                              <  1:3947292/150‑1 (MQ=255)
                                                                                                                         aTGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCGAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCtt                             <  1:3116905/150‑1 (MQ=255)
                                                                                                                         aTGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCtt                             >  2:2648912/1‑150 (MQ=255)
                                                                                                                         aTGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCtt                             <  2:2102297/150‑1 (MQ=255)
                                                                                                                          tGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTc                            >  2:3638846/1‑150 (MQ=255)
                                                                                                                          tGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTc                            >  2:4079842/1‑150 (MQ=255)
                                                                                                                          tGGGTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTc                            >  1:2860518/1‑150 (MQ=255)
                                                                                                                           gggTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCg                           <  2:1487521/150‑1 (MQ=255)
                                                                                                                             gTTAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGAt                         <  2:1228621/150‑1 (MQ=255)
                                                                                                                              ttAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATa                        <  1:3356082/150‑1 (MQ=255)
                                                                                                                               tAGCGCCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATAc                       >  1:1126059/1‑150 (MQ=255)
                                                                                                                                aGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATACt                      >  2:953124/1‑150 (MQ=255)
                                                                                                                                 gcgcCGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATACtt                     <  1:2302058/150‑1 (MQ=255)
                                                                                                                                   gcCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATACtttt                   >  1:81954/1‑150 (MQ=255)
                                                                                                                                     cGAGCATTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATACTTTTAc                 >  2:1622477/1‑150 (MQ=255)
                                                                                                                                       aGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATACTTTTACCg               <  1:3411437/150‑1 (MQ=255)
                                                                                                                                        gCATTTCGTAATCAGGAAGACTTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATACTTTTACCGa              <  2:2998168/150‑1 (MQ=255)
                                                                                                                                          aTTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATACTTTTACCGAAc            >  2:3937400/1‑150 (MQ=255)
                                                                                                                                          aTTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATACTTTTACCGAAc            >  2:793455/1‑150 (MQ=255)
                                                                                                                                          aTTTCGTAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATACTTTTACCGAAc            >  2:2054183/1‑150 (MQ=255)
                                                                                                                                            ttCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATACTTTTACCGAACtt          <  2:2736846/150‑1 (MQ=255)
                                                                                                                                               gTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATACTTTTACCGAACTTAcc       >  1:459193/1‑150 (MQ=255)
                                                                                                                                                tAAACAGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATACTTTTACCGAACTTACCg      >  1:1429196/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTCTTCGAACTGACCGCCTGATCCTTCGATACTTTTACCGAACTTACCGAGGA  >  CP009273/2561072‑2561369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑