Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,568,720 C→T 100% A33T (GCC→ACC)  eutP ← putative P‑loop NTPase ethanolamine utilization protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,568,7200CT98.8% 886.6 / ‑0.1 250A33T (GCC→ACC) eutPputative P‑loop NTPase ethanolamine utilization protein
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (109/137);  total (112/138)
Fisher's exact test for biased strand distribution p-value = 5.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 250 total aligned reads displayed.

GCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGTATAATTTAATA  >  CP009273/2568573‑2568866
                                                                                                                                                   |                                                                                                                                                  
gcaACCGACTTTCCGGGTCATTTGCGCCATGGACATAGCTCAACATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCt                                                                                                                                                  <  2:1611955/150‑1 (MQ=255)
gcaACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCt                                                                                                                                                  >  1:2028423/1‑150 (MQ=255)
 caACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCtg                                                                                                                                                 >  2:2018017/1‑150 (MQ=255)
      gACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGttt                                                                                                                                            >  1:3405480/1‑150 (MQ=255)
       aCTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGtttt                                                                                                                                           <  1:1636986/150‑1 (MQ=255)
       aCTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGtttt                                                                                                                                           <  1:2151700/150‑1 (MQ=255)
       aCTGTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGtttt                                                                                                                                           <  2:1509713/150‑1 (MQ=255)
          ttCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCt                                                                                                                                        >  1:69137/1‑150 (MQ=255)
           tCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTg                                                                                                                                       <  1:1020389/150‑1 (MQ=255)
           tCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTg                                                                                                                                       <  1:2634121/150‑1 (MQ=255)
               ggTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGa                                                                                                                                   >  2:2699078/1‑150 (MQ=255)
                  cATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAggg                                                                                                                                <  1:4000681/150‑1 (MQ=255)
                   aTTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGt                                                                                                                               <  1:1476363/150‑1 (MQ=255)
                    tttGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGta                                                                                                                              <  1:298805/150‑1 (MQ=255)
                     ttGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGtat                                                                                                                             <  2:199978/150‑1 (MQ=255)
                     ttGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAAATAAGGCGTGATTCCAGCTGGGATGGTTTAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGtat                                                                                                                             <  2:2399206/150‑1 (MQ=255)
                      tGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGtata                                                                                                                            <  1:1829467/150‑1 (MQ=255)
                        cgcCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAAt                                                                                                                          <  1:227579/150‑1 (MQ=255)
                        cgcCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAAt                                                                                                                          <  1:3918324/150‑1 (MQ=255)
                         gcCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAAtt                                                                                                                         <  1:618082/150‑1 (MQ=255)
                             tGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTcccc                                                                                                                     <  2:38750/150‑1 (MQ=255)
                              ggACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCt                                                                                                                    <  1:3106273/150‑1 (MQ=255)
                                 cATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCa                                                                                                                 <  2:2067017/150‑1 (MQ=255)
                                  aTAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCaa                                                                                                                >  2:1182953/1‑150 (MQ=255)
                                   tAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAAc                                                                                                               <  2:2606249/150‑1 (MQ=255)
                                     gATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGc                                                                                                             >  2:572502/1‑150 (MQ=255)
                                      aTCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCa                                                                                                            >  1:3894626/1‑150 (MQ=255)
                                        cAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCAtt                                                                                                          >  2:3838377/1‑150 (MQ=255)
                                         aGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTa                                                                                                         <  2:706400/150‑1 (MQ=255)
                                         aGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTa                                                                                                         <  1:2212857/150‑1 (MQ=255)
                                         aGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTa                                                                                                         <  1:1281971/150‑1 (MQ=255)
                                          gCATGTCTACATCCTGTAGCGTGGTTATTATGGAGTGATACCAGCGGGGATTGTTAAAATTTTCACCCGTCGTATCAATATCGCCCTTATCATTAAATTCCACGGTATGTGTTTTTCTGGAGAGGGTATAATTCCCCTGCAACGCTTTaa                                                                                                        <  2:2969869/150‑1 (MQ=255)
                                          gCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTaa                                                                                                        >  2:2188410/1‑150 (MQ=255)
                                             tGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATa                                                                                                     <  2:797358/150‑1 (MQ=255)
                                             tGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATa                                                                                                     <  2:3765439/150‑1 (MQ=255)
                                               tCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAAc                                                                                                   <  2:2574139/150‑1 (MQ=255)
                                                 tACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACgt                                                                                                 >  2:1383050/1‑150 (MQ=255)
                                                    aTCCTGTAGCGTGGTTATTAAGGAGTGATACCAGCTGGGTTGGTTATAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACgttgt                                                                                              <  1:3391930/150‑1 (MQ=255)
                                                    aTCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACgttgt                                                                                              >  2:3050384/1‑150 (MQ=255)
                                                    aTCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACgttgt                                                                                              >  2:1110756/1‑150 (MQ=255)
                                                     tCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACgttgtt                                                                                             <  1:1582694/150‑1 (MQ=255)
                                                      ccTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGttt                                                                                            >  2:989011/1‑150 (MQ=255)
                                                      ccTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGttt                                                                                            >  2:1003891/1‑150 (MQ=255)
                                                        tGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGttttt                                                                                          >  2:3075451/1‑150 (MQ=255)
                                                         gTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTc                                                                                         <  1:3082210/150‑1 (MQ=255)
                                                          tAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTcc                                                                                        <  2:3271429/150‑1 (MQ=255)
                                                           aGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCt                                                                                       <  1:4057016/150‑1 (MQ=255)
                                                           aGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCt                                                                                       >  2:348860/1‑150 (MQ=255)
                                                              gTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGcc                                                                                    >  1:93042/1‑150 (MQ=255)
                                                              gTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCCGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGcc                                                                                    >  1:3732280/1‑150 (MQ=255)
                                                                ggTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGcccc                                                                                  >  2:2087127/1‑150 (MQ=255)
                                                                   aaTTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGAc                                                                               <  1:1221880/150‑1 (MQ=255)
                                                                       aaGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTa                                                                           >  2:1018672/1‑150 (MQ=255)
                                                                          gCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTAccc                                                                        <  1:871635/150‑1 (MQ=255)
                                                                           cGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCca                                                                       <  2:547165/150‑1 (MQ=255)
                                                                           cGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAAGTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCca                                                                       <  2:2349272/150‑1 (MQ=255)
                                                                              gATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACaa                                                                    >  2:4063771/1‑150 (MQ=255)
                                                                               aTACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACaaa                                                                   >  2:3328733/1‑150 (MQ=255)
                                                                               aTACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACaaa                                                                   >  2:3604567/1‑150 (MQ=255)
                                                                               aTACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACaaa                                                                   >  2:2510924/1‑150 (MQ=255)
                                                                                tACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAAc                                                                  <  1:3621257/150‑1 (MQ=255)
                                                                                    aGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCaa                                                              >  2:2734048/1‑150 (MQ=255)
                                                                                        gggATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCg                                                          >  2:1827237/1‑150 (MQ=255)
                                                                                         ggATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGt                                                         >  1:2045365/1‑150 (MQ=255)
                                                                                             ggTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCa                                                     >  1:2978385/1‑150 (MQ=255)
                                                                                              gTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCAt                                                    <  1:1877289/150‑1 (MQ=255)
                                                                                              gTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCAt                                                    <  1:2466742/150‑1 (MQ=255)
                                                                                              gTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCAt                                                    <  2:189221/150‑1 (MQ=255)
                                                                                              gTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCAt                                                    >  1:1398017/1‑150 (MQ=255)
                                                                                               ttAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATg                                                   <  1:518603/150‑1 (MQ=255)
                                                                                                 aaaaTATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGaa                                                 <  2:3280323/150‑1 (MQ=255)
                                                                                                  aaaTATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAAt                                                <  2:4078745/150‑1 (MQ=255)
                                                                                                  aaaTATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAAt                                                >  1:2529755/1‑150 (MQ=255)
                                                                                                          aCCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTa                                        >  1:1034918/1‑150 (MQ=255)
                                                                                                           cccGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTAt                                       >  1:4083263/1‑150 (MQ=255)
                                                                                                                cGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACt                                  >  2:2454841/1‑150 (MQ=255)
                                                                                                                 gTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACtt                                 <  1:2328294/150‑1 (MQ=255)
                                                                                                                 gTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACtt                                 >  1:910112/1‑150 (MQ=255)
                                                                                                                  tATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACttt                                <  2:957464/150‑1 (MQ=255)
                                                                                                                  tATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACttt                                <  2:1598758/150‑1 (MQ=255)
                                                                                                                   aTCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACtttt                               >  2:2184801/1‑150 (MQ=255)
                                                                                                                   aTCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACtttt                               <  2:1438832/150‑1 (MQ=255)
                                                                                                                    tCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTg                              <  1:2540399/150‑1 (MQ=255)
                                                                                                                      aaTATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGt                            >  2:3406242/1‑150 (MQ=255)
                                                                                                                      aaTATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGt                            >  2:3406204/1‑150 (MQ=255)
                                                                                                                         atCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCAt                         <  1:3545704/150‑1 (MQ=255)
                                                                                                                           cGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCAttt                       <  2:2722461/150‑1 (MQ=255)
                                                                                                                            gCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTc                      >  2:3527457/1‑150 (MQ=255)
                                                                                                                                  aTCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAg                <  2:1338003/150‑1 (MQ=255)
                                                                                                                                  aTCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAg                <  1:3582559/150‑1 (MQ=255)
                                                                                                                                    cATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCg              <  2:49559/150‑1 (MQ=255)
                                                                                                                                     aTTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGt             <  1:1195567/150‑1 (MQ=255)
                                                                                                                                     aTTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGt             >  1:399251/1‑150 (MQ=255)
                                                                                                                                      ttAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGta            <  1:3040250/150‑1 (MQ=255)
                                                                                                                                        aaaTTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGtata          >  1:2919636/1‑150 (MQ=255)
                                                                                                                                        aaaTTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGtata          <  2:2991875/150‑1 (MQ=255)
                                                                                                                                        aaaTTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGtata          >  2:285493/1‑150 (MQ=255)
                                                                                                                                        aaaTTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGtaaa          >  2:62484/1‑148 (MQ=255)
                                                                                                                                            tCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGTATAAttt      <  2:317961/150‑1 (MQ=255)
                                                                                                                                             ccACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGTATAATTta     >  1:2416268/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGTATAATTTAATA  >  CP009273/2568573‑2568866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑