Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,612,335 C→A 14.5% A395A (GCG→GCT)  uraA ← uracil permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,612,3350CA14.5% 729.2 / 71.1 221A395A (GCG→GCT) uraAuracil permease
Reads supporting (aligned to +/- strand):  ref base C (86/103);  new base A (16/16);  total (102/119)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 228 total aligned reads displayed.

CCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACC  >  CP009273/2612186‑2612484
                                                                                                                                                     |                                                                                                                                                     
cccATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTc                                                                                                                                                       >  1:3191024/1‑150 (MQ=255)
   atgatgTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAATCCGATACCGACGATGGTCGcc                                                                                                                                                    >  1:3631954/1‑150 (MQ=255)
    tgatgTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCa                                                                                                                                                   <  1:3502017/150‑1 (MQ=255)
     gatgTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAg                                                                                                                                                  <  2:3404536/150‑1 (MQ=255)
       tgTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAgcg                                                                                                                                                >  1:2124978/1‑150 (MQ=255)
        gTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAgcgc                                                                                                                                               <  1:1063172/150‑1 (MQ=255)
          ttGACCCGGGCAGCACCACTGCCCGGTCGGTACATTATTTGCCTGTTATCTCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCa                                                                                                                                             <  2:3446161/150‑1 (MQ=255)
          ttGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTAGCCAGCGCCa                                                                                                                                             <  2:277732/150‑1 (MQ=255)
           tGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCAt                                                                                                                                            >  1:3374960/1‑150 (MQ=255)
           tGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCAt                                                                                                                                            <  1:2307828/150‑1 (MQ=255)
                 gggCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCttt                                                                                                                                      >  2:1479748/1‑150 (MQ=255)
                  ggCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCtttt                                                                                                                                     <  1:3681253/150‑1 (MQ=255)
                  ggCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTAGCCAGCGCCATACCtttt                                                                                                                                     >  1:2074887/1‑150 (MQ=255)
                     agcaACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAAc                                                                                                                                  >  1:474224/1‑150 (MQ=255)
                       caACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTc                                                                                                                                <  2:2451166/150‑1 (MQ=255)
                        aaCACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTcc                                                                                                                               <  1:2558819/150‑1 (MQ=255)
                         acacTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTccg                                                                                                                              >  1:1680312/1‑150 (MQ=255)
                          cacTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTccgc                                                                                                                             >  1:948998/1‑150 (MQ=255)
                           acTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTccgcc                                                                                                                            >  2:2576733/1‑150 (MQ=255)
                           acTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTccgcc                                                                                                                            >  1:790781/1‑150 (MQ=255)
                             tGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCCGCGCCATACCTTTTAACTCcgccgc                                                                                                                          >  2:2518904/1‑150 (MQ=255)
                                   gtcggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGAt                                                                                                                    <  2:809390/150‑1 (MQ=255)
                                    tcggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATg                                                                                                                   <  2:719077/150‑1 (MQ=255)
                                       gtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGttt                                                                                                                <  2:3808577/150‑1 (MQ=255)
                                         aCATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTAc                                                                                                              <  2:2947778/150‑1 (MQ=255)
                                          cATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTAcc                                                                                                             >  2:1691190/1‑150 (MQ=255)
                                          cATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTAcc                                                                                                             >  1:390682/1‑150 (MQ=255)
                                           attattTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCt                                                                                                            >  2:2058023/1‑150 (MQ=255)
                                                 tGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTAGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCgcgc                                                                                                      >  2:2851705/1‑150 (MQ=255)
                                                   tCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCa                                                                                                    <  2:1590556/150‑1 (MQ=255)
                                                   tCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCa                                                                                                    <  1:573521/150‑1 (MQ=255)
                                                    cTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCAc                                                                                                   >  2:2509542/1‑150 (MQ=255)
                                                      gTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCTCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTg                                                                                                 <  2:3701089/150‑1 (MQ=255)
                                                           atCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGcc                                                                                            <  2:1106054/150‑1 (MQ=255)
                                                           atCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGcc                                                                                            >  2:4106048/1‑150 (MQ=255)
                                                            tCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCg                                                                                           <  2:3013441/150‑1 (MQ=255)
                                                             ccGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCga                                                                                          <  2:2200462/150‑1 (MQ=255)
                                                              cgcgTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCgat                                                                                         >  2:3543887/1‑150 (MQ=255)
                                                               gcgTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCgatg                                                                                        <  2:1840058/150‑1 (MQ=255)
                                                                cgTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCgatga                                                                                       >  1:1483239/1‑150 (MQ=255)
                                                                 gTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCgatgat                                                                                      >  1:407434/1‑150 (MQ=255)
                                                                 gTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCgatgat                                                                                      >  1:3727797/1‑150 (MQ=255)
                                                                 gTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCgatgat                                                                                      <  2:617420/150‑1 (MQ=255)
                                                                 gTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCgatgat                                                                                      <  1:1330726/150‑1 (MQ=255)
                                                                   cttctGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTAGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCa                                                                                    >  2:959851/1‑150 (MQ=255)
                                                                      ctGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCaaaa                                                                                 <  1:2733304/150‑1 (MQ=255)
                                                                       tGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTAGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAAt                                                                                <  2:1708700/150‑1 (MQ=255)
                                                                            ccAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACgg                                                                           >  2:3608987/1‑150 (MQ=255)
                                                                            ccAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACgg                                                                           <  2:1996306/150‑1 (MQ=255)
                                                                            ccAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACgg                                                                           >  2:4565/1‑150 (MQ=255)
                                                                              aGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGaa                                                                         <  1:3338337/150‑1 (MQ=255)
                                                                              aGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGaa                                                                         <  1:3791948/150‑1 (MQ=255)
                                                                               gCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAg                                                                        <  1:2763801/150‑1 (MQ=255)
                                                                                 accacTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTc                                                                      <  1:51168/150‑1 (MQ=255)
                                                                                   cacTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAg                                                                    >  1:2285466/1‑150 (MQ=255)
                                                                                   cacTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAg                                                                    >  1:576571/1‑150 (MQ=255)
                                                                                   cacTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTAGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAg                                                                    >  1:1298733/1‑150 (MQ=255)
                                                                                      ttcttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGAt                                                                 <  1:2700208/150‑1 (MQ=255)
                                                                                       tcttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATc                                                                >  1:3692545/1‑150 (MQ=255)
                                                                                        cttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTAGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCa                                                               <  2:1346485/150‑1 (MQ=255)
                                                                                          tcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAgg                                                             <  2:1734459/150‑1 (MQ=255)
                                                                                           cAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGt                                                            >  2:2738380/1‑150 (MQ=255)
                                                                                            aGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGtt                                                           <  2:2555414/150‑1 (MQ=255)
                                                                                            aGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTAAAGCCGATAACGACGAAGGACGCCAGCGCCATACAATAAAACTCCACCGAGCCGAACTTAACCATCGCGACACACACGCCGATGATCAAAAACACGTAAGTCAGTATCacgat                                                           >  2:657379/1‑146 (MQ=255)
                                                                                             gggCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTc                                                          >  2:3664511/1‑150 (MQ=255)
                                                                                             gggCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTAGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTc                                                          >  1:41953/1‑150 (MQ=255)
                                                                                                cgcAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCACGATCAGGTTCtgt                                                       >  1:1787062/1‑150 (MQ=255)
                                                                                                  cagcaCACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTAGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGc                                                     >  2:3232336/1‑150 (MQ=255)
                                                                                                   agcaCACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGGTCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCt                                                    >  2:1500792/1‑150 (MQ=255)
                                                                                                   agcaCACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCt                                                    >  1:134375/1‑150 (MQ=255)
                                                                                                   agcaCACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCt                                                    <  2:3885369/150‑1 (MQ=255)
                                                                                                         cTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTttgttg                                              <  1:582702/150‑1 (MQ=255)
                                                                                                           gATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTa                                            <  2:1618561/150‑1 (MQ=255)
                                                                                                           gATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTa                                            <  2:2402288/150‑1 (MQ=255)
                                                                                                            aTCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTaa                                           <  2:2359431/150‑1 (MQ=255)
                                                                                                                 tttGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCAc                                      <  2:1511015/150‑1 (MQ=255)
                                                                                                                    gAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACttt                                   >  2:3122638/1‑150 (MQ=255)
                                                                                                                          cAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCACAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTttcgattc                             <  2:191187/150‑1 (MQ=255)
                                                                                                                          cAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTttcgattc                             <  1:791568/150‑1 (MQ=255)
                                                                                                                            aaCTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACAGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTttcgattcga                           >  2:3515903/1‑150 (MQ=255)
                                                                                                                             aCTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTtcgattcgat                          >  1:2622630/1‑150 (MQ=255)
                                                                                                                               ttAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCa                        >  1:3840795/1‑150 (MQ=255)
                                                                                                                                    ccGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAaca                   >  1:3131775/1‑150 (MQ=255)
                                                                                                                                    ccGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAaca                   >  1:2403679/1‑150 (MQ=255)
                                                                                                                                      gATACCGACGATGGTAGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACg                 >  2:3271724/1‑150 (MQ=255)
                                                                                                                                        tACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGaa               >  1:1612259/1‑150 (MQ=255)
                                                                                                                                        tACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGaa               >  1:1612284/1‑150 (MQ=255)
                                                                                                                                        tACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGaa               >  1:2890909/1‑150 (MQ=255)
                                                                                                                                        tACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGaa               <  2:2296352/150‑1 (MQ=255)
                                                                                                                                         aCCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAAt              >  2:116594/1‑150 (MQ=255)
                                                                                                                                         aCCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAAt              >  2:116625/1‑150 (MQ=255)
                                                                                                                                         aCCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAAt              <  1:3114861/150‑1 (MQ=255)
                                                                                                                                         aCCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAAt              >  1:3361542/1‑150 (MQ=255)
                                                                                                                                          ccGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATa             >  1:682821/1‑150 (MQ=255)
                                                                                                                                           cgacgaTGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATAc            <  2:4119971/150‑1 (MQ=255)
                                                                                                                                            gacgaTGGTCGCCAGCGCCATACCTTTTAACTCCGCCCCGCCGATGTTTACCTTCGCGCCACTGACGACGATGATCAAAATCACGGAAGTAAGGATCAGGTTCTGTGCTTTGTTGAAATCCACTTTCGATTCGATCAAAACACGAAAAcc           >  2:575330/1‑150 (MQ=255)
                                                                                                                                             acgaTGGTAGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCg          <  2:2920147/150‑1 (MQ=255)
                                                                                                                                              cgaTGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCgg         <  1:1920985/150‑1 (MQ=255)
                                                                                                                                                    tCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCAc   <  1:1936247/150‑1 (MQ=255)
                                                                                                                                                     cGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCAcc  <  2:536322/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACC  >  CP009273/2612186‑2612484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑