Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 232,860 G→T 100% D103Y (GAT→TAT)  dnaQ → DNA polymerase III epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273232,8600GT99.2% 841.0 / ‑1.4 237D103Y (GAT→TAT) dnaQDNA polymerase III epsilon subunit
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (103/132);  total (104/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 238 total aligned reads displayed.

CCCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCG  >  CP009273/232713‑233005
                                                                                                                                                   |                                                                                                                                                 
 ccGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCtata                                                                                                                                                >  2:3240613/1‑150 (MQ=255)
  cGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTatat                                                                                                                                               <  1:3055531/150‑1 (MQ=255)
  cGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTatat                                                                                                                                               <  1:2662261/150‑1 (MQ=255)
   taTCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGTTGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATc                                                                                                                                              <  2:636904/149‑1 (MQ=255)
   gATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATc                                                                                                                                              >  2:1664016/1‑150 (MQ=255)
   gATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATc                                                                                                                                              >  2:106184/1‑150 (MQ=255)
       ggCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCt                                                                                                                                          >  2:198422/1‑150 (MQ=255)
       ggCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCt                                                                                                                                          >  2:872107/1‑150 (MQ=255)
       ggCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCt                                                                                                                                          >  2:3215829/1‑150 (MQ=255)
       ggCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCt                                                                                                                                          >  2:1151884/1‑150 (MQ=255)
        gCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTAATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCtt                                                                                                                                         >  1:3366082/1‑150 (MQ=255)
         cTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCttt                                                                                                                                        <  2:2369360/150‑1 (MQ=255)
             tggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATgg                                                                                                                                    <  2:886485/150‑1 (MQ=255)
                aTCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACt                                                                                                                                 <  2:464599/150‑1 (MQ=255)
                  ccGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTAc                                                                                                                               <  1:2001953/150‑1 (MQ=255)
                   cGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTGATGGACTACg                                                                                                                              >  2:933334/1‑150 (MQ=255)
                    ggAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGa                                                                                                                             <  1:2693871/150‑1 (MQ=255)
                       aGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGtt                                                                                                                          <  2:2079082/150‑1 (MQ=255)
                          cTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTc                                                                                                                       >  1:640562/1‑150 (MQ=255)
                            ttGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGt                                                                                                                     <  1:1341464/150‑1 (MQ=255)
                             tGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGtt                                                                                                                    <  2:2537252/150‑1 (MQ=255)
                              ggCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTg                                                                                                                   >  2:2613475/1‑150 (MQ=255)
                              ggCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTg                                                                                                                   >  2:3320442/1‑150 (MQ=255)
                                 gTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCtt                                                                                                                >  2:436042/1‑150 (MQ=255)
                                  tACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTa                                                                                                               >  2:1847413/1‑150 (MQ=255)
                                   aCATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTaa                                                                                                              <  1:3103779/150‑1 (MQ=255)
                                      tGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcg                                                                                                           <  1:1612133/150‑1 (MQ=255)
                                       ggTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                                                                                                          >  2:2608180/1‑150 (MQ=255)
                                        gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGcgcg                                                                                                         <  1:1442167/150‑1 (MQ=255)
                                          aTTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGat                                                                                                       <  1:626794/150‑1 (MQ=255)
                                           ttGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGata                                                                                                      <  2:3749025/150‑1 (MQ=255)
                                             gCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt                                                                                                    >  1:2357559/1‑150 (MQ=255)
                                             gCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt                                                                                                    <  1:2462460/150‑1 (MQ=255)
                                             gCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt                                                                                                    >  1:360372/1‑150 (MQ=255)
                                              ccGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTc                                                                                                   >  1:941659/1‑150 (MQ=255)
                                                 aTGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa                                                                                                >  1:2840193/1‑150 (MQ=255)
                                                  tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGaa                                                                                               <  2:2701271/150‑1 (MQ=255)
                                                   gAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAg                                                                                              >  2:3918978/1‑150 (MQ=255)
                                                     aTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGAc                                                                                            <  1:509850/150‑1 (MQ=255)
                                                     aTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGAc                                                                                            <  1:534146/150‑1 (MQ=255)
                                                       ttttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCa                                                                                          <  2:317204/150‑1 (MQ=255)
                                                       ttttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCa                                                                                          <  1:1380244/150‑1 (MQ=255)
                                                          tGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATa                                                                                       >  1:3293924/1‑150 (MQ=255)
                                                           gCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATAc                                                                                      <  2:407907/150‑1 (MQ=255)
                                                           gCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATAc                                                                                      <  2:1257800/150‑1 (MQ=255)
                                                             tCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACtt                                                                                    <  1:3869286/150‑1 (MQ=255)
                                                               gATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTc                                                                                  >  1:2924895/1‑150 (MQ=255)
                                                               gATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTc                                                                                  >  2:1531729/1‑150 (MQ=255)
                                                               gATAAGCCCACGTTTGCAGAAGTAGCCGATGAGTTCATGGAATATATTAGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCAGAAGACCAATACTTTc                                                                                  >  1:3552669/1‑150 (MQ=255)
                                                                aTAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCt                                                                                 <  2:4099862/150‑1 (MQ=255)
                                                                aTAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCt                                                                                 >  2:1591270/1‑150 (MQ=255)
                                                                aTAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCt                                                                                 <  2:2696338/150‑1 (MQ=255)
                                                                  aaGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATActgtctgt                                                                               >  2:2098056/1‑150 (MQ=255)
                                                                  aaGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGt                                                                               >  1:2643796/1‑150 (MQ=255)
                                                                        aCGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTc                                                                         <  2:3567776/150‑1 (MQ=255)
                                                                          gTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCAc                                                                       >  2:3900179/1‑150 (MQ=255)
                                                                            ttGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCg                                                                     <  2:1450637/150‑1 (MQ=255)
                                                                             tGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGa                                                                    <  1:3165543/150‑1 (MQ=255)
                                                                              gCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGAt                                                                   <  2:2014237/150‑1 (MQ=255)
                                                                              gCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGAt                                                                   <  1:1273133/150‑1 (MQ=255)
                                                                               ccGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATa                                                                  <  2:2501077/150‑1 (MQ=255)
                                                                                cGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAg                                                                 <  1:295458/150‑1 (MQ=255)
                                                                                cGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAg                                                                 <  2:716370/150‑1 (MQ=255)
                                                                                  aaGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGcc                                                               >  1:1842184/1‑150 (MQ=255)
                                                                                    gTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCtt                                                             <  1:446994/150‑1 (MQ=255)
                                                                                       gCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCg                                                          >  1:1212800/1‑150 (MQ=255)
                                                                                         cGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGt                                                        >  1:2141433/1‑150 (MQ=255)
                                                                                          gATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTg                                                       <  1:2846069/150‑1 (MQ=255)
                                                                                              aGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGa                                                   >  1:4023512/1‑150 (MQ=255)
                                                                                              aGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGa                                                   >  2:1731624/1‑150 (MQ=255)
                                                                                               gTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAg                                                  >  1:4117616/1‑150 (MQ=255)
                                                                                                  cATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGaa                                               >  2:3054149/1‑150 (MQ=255)
                                                                                                  cATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGaa                                               <  1:533776/150‑1 (MQ=255)
                                                                                                   aTGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGaaa                                              >  1:2593981/1‑150 (MQ=255)
                                                                                                     ggACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAAt                                            >  2:3751384/1‑150 (MQ=255)
                                                                                                        cTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGtt                                         >  2:3415082/1‑150 (MQ=255)
                                                                                                         tataTTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGttt                                        >  2:2632542/1‑150 (MQ=255)
                                                                                                          atatTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTc                                       <  2:378492/150‑1 (MQ=255)
                                                                                                          atagTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTc                                       <  2:35939/150‑1 (MQ=255)
                                                                                                           tatTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGCAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTcc                                      <  2:1643664/150‑1 (MQ=255)
                                                                                                           tatTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTcc                                      <  2:1810375/150‑1 (MQ=255)
                                                                                                            atTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTccc                                     >  2:2739442/1‑150 (MQ=255)
                                                                                                            atTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTccc                                     >  2:532704/1‑150 (MQ=255)
                                                                                                            atTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTccc                                     <  1:705887/150‑1 (MQ=255)
                                                                                                               cgcggcgcggAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGt                                  >  2:1323150/1‑150 (MQ=255)
                                                                                                                 cggcgcggAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTaa                                <  2:2207194/150‑1 (MQ=255)
                                                                                                                  ggcgcggAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAg                               >  2:1390669/1‑150 (MQ=255)
                                                                                                                         aGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACa                        <  1:142119/150‑1 (MQ=255)
                                                                                                                               tGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCg                  >  1:3482210/1‑150 (MQ=255)
                                                                                                                                 aTCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGAt                <  1:1111750/150‑1 (MQ=255)
                                                                                                                                 aTCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGAt                <  2:773331/150‑1 (MQ=255)
                                                                                                                                  tCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAATATGTTTCCCGGTAAGCGCAACAGCCTCGATg               >  2:2803203/1‑150 (MQ=255)
                                                                                                                                  tCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATg               >  1:2253293/1‑150 (MQ=255)
                                                                                                                                  tCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATg               <  1:1914140/150‑1 (MQ=255)
                                                                                                                                    cATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCg             <  2:3978219/150‑1 (MQ=255)
                                                                                                                                      tAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGtt           >  2:1878261/1‑150 (MQ=255)
                                                                                                                                       aaCGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTa          <  1:1507730/150‑1 (MQ=255)
                                                                                                                                        aCGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTAt         <  2:997143/150‑1 (MQ=255)
                                                                                                                                              cGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTc   <  1:426264/150‑1 (MQ=255)
                                                                                                                                              cGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTc   >  2:3558629/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCG  >  CP009273/232713‑233005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑