Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,651,665 G→T 100% G210G (GGC→GGA)  hscA ← DnaK‑like molecular chaperone specific for IscU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,651,6650GT99.8% 975.9 / ‑6.1 271G210G (GGC→GGA) hscADnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (155/115);  minor base A (1/0);  total (156/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 271 total aligned reads displayed.

GCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCA  >  CP009273/2651518‑2651809
                                                                                                                                                   |                                                                                                                                                
gCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTcc                                                                                                                                                >  1:2894111/1‑150 (MQ=255)
  tACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCAc                                                                                                                                              <  1:1693213/150‑1 (MQ=255)
   aCGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCAcc                                                                                                                                             >  1:3579115/1‑150 (MQ=255)
    cGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCg                                                                                                                                            <  2:1205033/150‑1 (MQ=255)
     gATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAACTCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGa                                                                                                                                           <  2:2031360/150‑1 (MQ=255)
       tCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAgg                                                                                                                                         <  1:3765057/150‑1 (MQ=255)
         aGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTc                                                                                                                                       >  2:1991306/1‑150 (MQ=255)
            aaTGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATa                                                                                                                                    <  2:2584556/150‑1 (MQ=255)
               gCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAAc                                                                                                                                 >  1:2977070/1‑150 (MQ=255)
                    ccTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGa                                                                                                                            >  2:3914/1‑150 (MQ=255)
                    ccTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGa                                                                                                                            >  2:1785249/1‑150 (MQ=255)
                     cTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGAt                                                                                                                           >  1:2786212/1‑150 (MQ=255)
                      tGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATc                                                                                                                          <  1:2493148/150‑1 (MQ=255)
                       gCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGACTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCa                                                                                                                         >  2:2134982/1‑150 (MQ=255)
                       gCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCa                                                                                                                         >  2:168466/1‑150 (MQ=255)
                       gCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCa                                                                                                                         >  1:889147/1‑150 (MQ=255)
                       gCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCa                                                                                                                         >  2:3425655/1‑150 (MQ=255)
                            cgAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCt                                                                                                                    <  2:3781583/150‑1 (MQ=255)
                             gAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCtt                                                                                                                   >  2:813618/1‑150 (MQ=255)
                               aTGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTcc                                                                                                                 >  1:1864291/1‑150 (MQ=255)
                                   aaTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGTAATCACCGCCGGTTGCCAGCACTACAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAACCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGAc                                                                                                             <  1:1039925/150‑1 (MQ=255)
                                   aaTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGAc                                                                                                             >  1:3715466/1‑150 (MQ=255)
                                   aaTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGAc                                                                                                             >  1:3840651/1‑150 (MQ=255)
                                   aaTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGAc                                                                                                             <  2:1000042/150‑1 (MQ=255)
                                   aaTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGAc                                                                                                             <  1:3396316/150‑1 (MQ=255)
                                    aTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGANGTCATAAACGGCGATCACGCCTTCCTGAcc                                                                                                            <  1:3853615/150‑1 (MQ=255)
                                    aTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGAcc                                                                                                            >  1:1307175/1‑150 (MQ=255)
                                    aTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGAcc                                                                                                            <  2:323948/150‑1 (MQ=255)
                                    aTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTAAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGAcc                                                                                                            <  2:4105255/150‑1 (MQ=255)
                                       cGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGa                                                                                                         <  1:4107678/150‑1 (MQ=255)
                                         ccAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAAt                                                                                                       >  1:2858464/1‑150 (MQ=255)
                                          cagcagATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATc                                                                                                      >  2:3657658/1‑150 (MQ=255)
                                           agcagATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATcc                                                                                                     >  1:1499248/1‑150 (MQ=255)
                                              agATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTGCAAACACGCCCCGAATAAAGCGCATAATGGAAAAATCAAACGACCCACAACCGAGGTCAAAAACGGCTAGCACGCCTACCTGACCGGAAACAAGc                                                                                                  >  1:635965/1‑150 (MQ=255)
                                               gATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGcc                                                                                                 <  2:4023605/150‑1 (MQ=255)
                                                aTGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGccc                                                                                                >  2:309283/1‑150 (MQ=255)
                                                 tGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCg                                                                                               <  2:2350854/150‑1 (MQ=255)
                                                    tCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAg                                                                                            >  1:310282/1‑150 (MQ=255)
                                                     cGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAgg                                                                                           >  1:228464/1‑150 (MQ=255)
                                                      gAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGc                                                                                          >  2:4080355/1‑150 (MQ=255)
                                                       aaaTCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCg                                                                                         >  2:3593194/1‑150 (MQ=255)
                                                       aaaTCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCg                                                                                         >  2:1765400/1‑150 (MQ=255)
                                                        aaTCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAATCACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGa                                                                                        >  2:2294253/1‑150 (MQ=255)
                                                        aaTCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGa                                                                                        <  1:3025592/150‑1 (MQ=255)
                                                           catcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAg                                                                                     <  1:193775/150‑1 (MQ=255)
                                                            atcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGc                                                                                    >  1:1110487/1‑150 (MQ=255)
                                                              cgccgcCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCg                                                                                  >  1:2297248/1‑150 (MQ=255)
                                                                ccgccgAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCa                                                                                >  2:1261659/1‑150 (MQ=255)
                                                                     gAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggt                                                                           >  1:23743/1‑150 (MQ=255)
                                                                      aGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggtc                                                                          >  2:2762172/1‑150 (MQ=255)
                                                                      aGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggtc                                                                          <  2:2734871/150‑1 (MQ=255)
                                                                            gAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCg                                                                    <  2:3199997/150‑1 (MQ=255)
                                                                             aaTCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGt                                                                   >  1:16215/1‑150 (MQ=255)
                                                                              aTCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGtt                                                                  <  2:2689962/150‑1 (MQ=255)
                                                                               tCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTta                                                                 >  2:619473/1‑150 (MQ=255)
                                                                                cACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaa                                                                >  1:3271623/1‑150 (MQ=255)
                                                                                   cgccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaagta                                                             <  2:148491/150‑1 (MQ=255)
                                                                                     ccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaagtaag                                                           <  1:3365363/150‑1 (MQ=255)
                                                                                     ccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaagtaag                                                           <  2:554742/150‑1 (MQ=255)
                                                                                     ccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaagtaag                                                           >  1:2831208/1‑150 (MQ=255)
                                                                                     ccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTGCGGtaagtaag                                                           >  1:2830928/1‑150 (MQ=255)
                                                                                           gCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAgg                                                     >  1:2602106/1‑150 (MQ=255)
                                                                                             cAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGAc                                                   >  2:97058/1‑150 (MQ=255)
                                                                                              aGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACg                                                  <  2:60041/150‑1 (MQ=255)
                                                                                                cACTTCAACCCCGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTg                                                <  1:402266/150‑1 (MQ=255)
                                                                                                 aCTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGa                                               >  2:1029916/1‑150 (MQ=255)
                                                                                                     cAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGc                                           <  2:2469611/150‑1 (MQ=255)
                                                                                                       aaCACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTACGCGCAGGACGTGAAGGccc                                         >  2:3034943/1‑150 (MQ=255)
                                                                                                         cacGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGc                                       >  1:2220083/1‑150 (MQ=255)
                                                                                                           cgccgcGACTTAAGCGCAGAATGGAAATATCCAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCa                                     <  2:3374573/150‑1 (MQ=255)
                                                                                                             ccgcGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGa                                   <  2:1613999/150‑1 (MQ=255)
                                                                                                             ccgcGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGa                                   <  1:372252/150‑1 (MQ=255)
                                                                                                               gcgACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGAcg                                 <  1:513574/150‑1 (MQ=255)
                                                                                                                cgACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGAcgc                                >  1:3247785/1‑150 (MQ=255)
                                                                                                                cgACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGAcgc                                >  1:1991628/1‑150 (MQ=255)
                                                                                                                 gACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGAcgcg                               >  2:4134920/1‑150 (MQ=255)
                                                                                                                 gACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGAcgcg                               <  1:1054382/150‑1 (MQ=255)
                                                                                                                  aCTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACgcgc                              >  1:94831/1‑150 (MQ=255)
                                                                                                                  aCTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACgcgc                              >  1:3936873/1‑150 (MQ=255)
                                                                                                                          gcAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCtt                      >  2:2958741/1‑150 (MQ=255)
                                                                                                                            aGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTg                    >  1:487421/1‑150 (MQ=255)
                                                                                                                            aGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTg                    >  2:19205/1‑150 (MQ=255)
                                                                                                                               aTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTg                 >  1:3314452/1‑150 (MQ=255)
                                                                                                                                tGGAAATATCATCCGTCCCTCCACCGAGGTCATACACGGCGATCACGCCGTCCTGACCGGAATCCCGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGc                <  2:352133/150‑1 (MQ=255)
                                                                                                                                tGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGc                <  1:733691/150‑1 (MQ=255)
                                                                                                                                 ggAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGcc               >  2:2539003/1‑150 (MQ=255)
                                                                                                                                    aaTATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTg            >  1:3659138/1‑150 (MQ=255)
                                                                                                                                      tatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGAc          <  1:675617/150‑1 (MQ=255)
                                                                                                                                      tatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGAc          >  2:1611111/1‑150 (MQ=255)
                                                                                                                                         cAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCt       >  1:575249/1‑150 (MQ=255)
                                                                                                                                          aaaCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTg      >  1:2106019/1‑150 (MQ=255)
                                                                                                                                          aaaCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTg      >  1:2103211/1‑150 (MQ=255)
                                                                                                                                          aaaCGTCCCTACACCGAGGTCATAAACGGCGATCACGCCTTCCTGAACGGAATCCATCCCGTAGGCGATAGCCGCAGCGCTCGGTACGGTAAGAAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCTCGTCTTTGGTGCCCTGACGCTg      >  2:1109155/1‑150 (MQ=255)
                                                                                                                                           taCGTCCCTCCACCGAGGTCATCCACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCTTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTgg     <  2:2221093/149‑1 (MQ=255)
                                                                                                                                             cGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGc   <  2:1287881/150‑1 (MQ=255)
                                                                                                                                              gTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCCGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCa  <  2:1426108/150‑1 (MQ=255)
                                                                                                                                              gTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCa  >  2:3679310/1‑150 (MQ=255)
                                                                                                                                              gTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCa  >  2:660374/1‑150 (MQ=255)
                                                                                                                                              gTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCa  >  1:1299933/1‑150 (MQ=255)
                                                                                                                                              gTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCa  >  2:1710791/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCA  >  CP009273/2651518‑2651809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑