Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,778,281 C→T 7.8% pseudogene (90/1374 nt) ypjC ← pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,778,2810CT 7.8% 887.7 / 42.1 255pseudogene (90/1374 nt)ypjCpseudogene
Reads supporting (aligned to +/- strand):  ref base C (109/126);  new base T (16/4);  total (125/130)
Fisher's exact test for biased strand distribution p-value = 4.55e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

AATTGTTCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCA  >  CP009273/2778132‑2778427
                                                                                                                                                      |                                                                                                                                                  
aaTTGTTCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGc                                                                                                                                                    >  2:470757/1‑150 (MQ=255)
   tGTTCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACCAAATTAATTTTAAAATACTATGAGATAGTAGCTc                                                                                                                                                  <  2:2684635/150‑1 (MQ=255)
   tGTTCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTcc                                                                                                                                                 >  1:3577945/1‑150 (MQ=255)
   tGTTCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTcc                                                                                                                                                 >  2:3231464/1‑150 (MQ=255)
    gTTCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCa                                                                                                                                                >  1:1143544/1‑150 (MQ=255)
     ttCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCAt                                                                                                                                               <  1:1023645/150‑1 (MQ=255)
     ttCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCAt                                                                                                                                               >  1:3598695/1‑150 (MQ=255)
     ttCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCAt                                                                                                                                               >  2:2884974/1‑150 (MQ=255)
      tctcCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCAtg                                                                                                                                              <  2:3441637/150‑1 (MQ=255)
      tctcCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCAtg                                                                                                                                              >  1:486727/1‑150 (MQ=255)
       ctcCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCAtgt                                                                                                                                             >  2:1360360/1‑150 (MQ=255)
          cAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGtt                                                                                                                                          >  1:13093/1‑150 (MQ=255)
           aGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGttt                                                                                                                                         <  2:3012306/150‑1 (MQ=255)
           aGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGttt                                                                                                                                         <  1:2530367/150‑1 (MQ=255)
           aGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGttt                                                                                                                                         >  1:2268501/1‑150 (MQ=255)
            gCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTa                                                                                                                                        <  1:2537084/150‑1 (MQ=255)
             cTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTag                                                                                                                                       <  2:2157483/150‑1 (MQ=255)
             cTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTag                                                                                                                                       >  1:2366152/1‑150 (MQ=255)
             cTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTag                                                                                                                                       <  2:2755820/150‑1 (MQ=255)
               cccATACTGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTagaa                                                                                                                                     <  2:223920/150‑1 (MQ=255)
                  aTAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGat                                                                                                                                  >  1:178142/1‑150 (MQ=255)
                   tAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGatt                                                                                                                                 >  1:2801273/1‑150 (MQ=255)
                   tAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGatt                                                                                                                                 <  2:3284842/150‑1 (MQ=255)
                     aTGACAAAGGCGTTGCCTTCTTGATATATACCTTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGattta                                                                                                                               <  2:403644/150‑1 (MQ=255)
                     aTGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGattta                                                                                                                               >  2:515263/1‑150 (MQ=255)
                       gACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGatttatt                                                                                                                             <  2:3917986/150‑1 (MQ=255)
                       gACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGatttatt                                                                                                                             <  2:2739518/150‑1 (MQ=255)
                         cAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTAtttt                                                                                                                           <  1:2466184/150‑1 (MQ=255)
                         cAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTAtttt                                                                                                                           >  2:3276/1‑150 (MQ=255)
                         cAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTAtttt                                                                                                                           <  2:3956867/150‑1 (MQ=255)
                         cAAAGGCGGTGCCTTCTTGATATATACCTGCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCGTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTAtttt                                                                                                                           <  2:4021818/150‑1 (MQ=255)
                          aaaGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTc                                                                                                                          >  1:4017166/1‑150 (MQ=255)
                           aaGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCa                                                                                                                         <  1:1245275/150‑1 (MQ=255)
                            aGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCAt                                                                                                                        <  1:2300441/150‑1 (MQ=255)
                            aGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCAt                                                                                                                        >  2:1490823/1‑150 (MQ=255)
                               cGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATtat                                                                                                                     >  1:1380438/1‑150 (MQ=255)
                               cGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATtat                                                                                                                     <  1:3158004/150‑1 (MQ=255)
                                gTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATtata                                                                                                                    <  2:2067872/150‑1 (MQ=255)
                                  tGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAAc                                                                                                                  >  1:2483433/1‑150 (MQ=255)
                                    ccTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACtt                                                                                                                >  1:2894683/1‑150 (MQ=255)
                                     cttcttGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTc                                                                                                               <  2:3356009/150‑1 (MQ=255)
                                     cttcttGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACAGCGATACGTCTCTGATTCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTc                                                                                                               <  1:2673047/150‑1 (MQ=255)
                                     cttcttGATATATACATTCAGACATCATTTTTTCTAAAGTTTCAAGAGCACCGCGATACGTCTCAGATGCAGCTTCCTTA‑CAAATTAATATTAAAATACAATGAGCTAGTAGCTCCAAGTGATTAGAAGATTTATATTCATTATAACTTc                                                                                                               >  2:375989/1‑150 (MQ=255)
                                         ttGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACt                                                                                                           <  2:1117361/150‑1 (MQ=255)
                                         ttGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACt                                                                                                           <  2:1873012/150‑1 (MQ=255)
                                          tGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAATAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTACATTATAACTTCCACTa                                                                                                          >  1:1604835/1‑150 (MQ=255)
                                             tatataCATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTAccc                                                                                                       >  2:1536217/1‑150 (MQ=255)
                                               tataCATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCaa                                                                                                     >  1:2945534/1‑150 (MQ=255)
                                                  aCATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAAc                                                                                                  <  1:983653/150‑1 (MQ=255)
                                                  aCATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAAc                                                                                                  >  1:3013500/1‑150 (MQ=255)
                                                        aGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCt                                                                                            >  2:3900190/1‑150 (MQ=255)
                                                          aCATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAg                                                                                          <  2:2443325/150‑1 (MQ=255)
                                                           catcatTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGc                                                                                         <  1:3202254/150‑1 (MQ=255)
                                                              catTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGtt                                                                                      >  2:2552690/1‑150 (MQ=255)
                                                              catTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGtt                                                                                      >  2:1653685/1‑150 (MQ=255)
                                                               atTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTa                                                                                     >  1:2187055/1‑150 (MQ=255)
                                                               atTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTa                                                                                     <  2:2927664/150‑1 (MQ=255)
                                                                 tttttGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTaaa                                                                                   <  2:3862448/150‑1 (MQ=255)
                                                                      gTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGt                                                                              >  1:3154565/1‑150 (MQ=255)
                                                                        aaaGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTg                                                                            >  1:3347880/1‑150 (MQ=255)
                                                                          aGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCt                                                                          >  1:316179/1‑150 (MQ=255)
                                                                           gTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCtt                                                                         >  2:636911/1‑150 (MQ=255)
                                                                            tttCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCttt                                                                        >  2:3514600/1‑150 (MQ=255)
                                                                               cAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTac                                                                     <  1:927566/150‑1 (MQ=255)
                                                                                aaGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTact                                                                    <  1:2907707/150‑1 (MQ=255)
                                                                                aaGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTact                                                                    <  1:725707/150‑1 (MQ=255)
                                                                                  gagCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTATAAAATACTATGATCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTAATAATAACTAACACTACCCAAAACTGCGCTAGCGTTAAATAAGTTGCTTTTactaa                                                                  >  1:638175/1‑150 (MQ=255)
                                                                                      aCCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTActaactaa                                                              >  1:2870187/1‑150 (MQ=255)
                                                                                         gcgATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACAt                                                           <  1:545980/150‑1 (MQ=255)
                                                                                            aTACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGt                                                        <  2:953714/150‑1 (MQ=255)
                                                                                            aTACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGt                                                        >  2:2017091/1‑150 (MQ=255)
                                                                                             tACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTc                                                       >  2:2299819/1‑150 (MQ=255)
                                                                                               cGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTa                                                     <  1:3326975/150‑1 (MQ=255)
                                                                                               cGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTa                                                     <  2:3066036/150‑1 (MQ=255)
                                                                                                gTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTAt                                                    <  2:3731516/150‑1 (MQ=255)
                                                                                                 tctcTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAATTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTAtt                                                   <  1:1651316/150‑1 (MQ=255)
                                                                                                 tctcTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTAtt                                                   <  2:3163168/150‑1 (MQ=255)
                                                                                                 tctcTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTAtt                                                   <  2:3305314/150‑1 (MQ=255)
                                                                                                          cAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTa                                          <  1:2147731/150‑1 (MQ=255)
                                                                                                            gCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAAt                                        >  2:3703210/1‑150 (MQ=255)
                                                                                                            gCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAAt                                        <  2:3644106/150‑1 (MQ=255)
                                                                                                                ccTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCt                                    <  1:833640/150‑1 (MQ=255)
                                                                                                                 cTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTa                                   >  2:2816867/1‑150 (MQ=255)
                                                                                                                      cAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATg                               <  2:803679/150‑1 (MQ=255)
                                                                                                                      cAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATg                               >  2:11544/1‑150 (MQ=255)
                                                                                                                       aaaTTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATgg                              <  1:2042012/150‑1 (MQ=255)
                                                                                                                            aattTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTTTATTCCTCAATTAATGTCTACATGGCTAtt                         <  1:3860633/150‑1 (MQ=255)
                                                                                                                             attTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTAttt                        >  1:884845/1‑150 (MQ=255)
                                                                                                                                  aaaaTACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAAt                   <  2:2147493/150‑1 (MQ=255)
                                                                                                                                  aaaaTACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAAt                   >  1:1692557/1‑150 (MQ=255)
                                                                                                                                  aaaaTACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAAt                   <  1:2772086/150‑1 (MQ=255)
                                                                                                                                   aaaTACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATg                  >  1:3696338/1‑150 (MQ=255)
                                                                                                                                   aaaTACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATg                  >  2:2570915/1‑150 (MQ=255)
                                                                                                                                   aaaTACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATg                  >  1:3696595/1‑150 (MQ=255)
                                                                                                                                      tACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGtta               >  2:382176/1‑150 (MQ=255)
                                                                                                                                      tACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGtta               >  1:1989964/1‑150 (MQ=255)
                                                                                                                                               cTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGttt      >  1:1527016/1‑150 (MQ=255)
                                                                                                                                                 agtagCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTgtgtgt    >  1:1746673/1‑150 (MQ=255)
                                                                                                                                                 agtagCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGt    >  1:3422925/1‑150 (MQ=255)
                                                                                                                                                   tagCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCa  >  2:2655915/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
AATTGTTCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTA‑CAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCA  >  CP009273/2778132‑2778427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑