Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,890,009 C→A 5.9% A407A (GCG→GCT)  ygcS ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,890,0090CA 5.9% 918.0 / 23.6 236A407A (GCG→GCT) ygcSputative transporter
Reads supporting (aligned to +/- strand):  ref base C (81/141);  new base A (10/4);  total (91/145)
Fisher's exact test for biased strand distribution p-value = 1.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

Only 100 of 238 total aligned reads displayed.

TTTCCGCCAGCATTCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAA  >  CP009273/2889860‑2890158
                                                                                                                                                     |                                                                                                                                                     
tttCCGCCAGCATTCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTc                                                                                                                                                       <  2:807169/150‑1 (MQ=255)
 ttCCGCCAGCATTCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTCCATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCg                                                                                                                                                      <  1:3768534/150‑1 (MQ=255)
 ttCCGCCAGCATTCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCg                                                                                                                                                      >  2:3166938/1‑150 (MQ=255)
   ccGCCAGCATTCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGcc                                                                                                                                                    <  2:2221015/150‑1 (MQ=255)
    cGCCAGCATTCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACCCTGTCGCCa                                                                                                                                                   <  2:3453427/150‑1 (MQ=255)
     gCCAGCATTCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAg                                                                                                                                                  <  1:1510717/150‑1 (MQ=255)
     gCCAGCATTCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAg                                                                                                                                                  <  2:1963447/150‑1 (MQ=255)
      ccAGCATTCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAgg                                                                                                                                                 <  1:2486705/150‑1 (MQ=255)
        aGCATTCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAg                                                                                                                                               <  1:357977/150‑1 (MQ=255)
        aGCATTCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAg                                                                                                                                               >  1:3674730/1‑150 (MQ=255)
        aGCATTCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAg                                                                                                                                               >  1:107639/1‑150 (MQ=255)
          cATTCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCCCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCa                                                                                                                                             <  1:2539073/150‑1 (MQ=255)
           aTTCCGGCATCCGGTTCGGCTTTAAACGCGAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCaa                                                                                                                                            >  1:891756/1‑150 (MQ=255)
            ttCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAAt                                                                                                                                           <  2:1433666/150‑1 (MQ=255)
            ttCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAAt                                                                                                                                           >  1:2177252/1‑150 (MQ=255)
             tCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCAAGGAGCAATa                                                                                                                                          <  1:1324303/150‑1 (MQ=255)
                ggCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAgg                                                                                                                                       <  2:1757735/150‑1 (MQ=255)
                   aTCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGa                                                                                                                                    <  1:3884336/150‑1 (MQ=255)
                      cGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACtt                                                                                                                                 <  1:2938821/150‑1 (MQ=255)
                         ttCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCa                                                                                                                              >  1:1905852/1‑150 (MQ=255)
                         ttCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCa                                                                                                                              >  1:1678374/1‑150 (MQ=255)
                          tCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCAt                                                                                                                             <  2:3850216/150‑1 (MQ=255)
                          tCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCAt                                                                                                                             <  1:1876503/150‑1 (MQ=255)
                           cGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCAtt                                                                                                                            >  2:2900431/1‑150 (MQ=255)
                             gCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTcc                                                                                                                          <  2:1621948/150‑1 (MQ=255)
                              cTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTccc                                                                                                                         <  1:3763418/150‑1 (MQ=255)
                              cTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTccc                                                                                                                         <  2:3393223/150‑1 (MQ=255)
                                ttAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCa                                                                                                                       <  2:2657233/150‑1 (MQ=255)
                                ttAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCa                                                                                                                       >  2:943383/1‑150 (MQ=255)
                                 tAAACGGTTACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACACCACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCAc                                                                                                                      <  2:146699/150‑1 (MQ=255)
                                 tAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCAc                                                                                                                      <  1:2742605/150‑1 (MQ=255)
                                   aaCGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTg                                                                                                                    >  2:1851213/1‑150 (MQ=255)
                                    aCGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTgc                                                                                                                   <  1:3473738/150‑1 (MQ=255)
                                        tAACAGAATGTTCATTCGCACCTCCTCCATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCTCCa                                                                                                               <  2:4045741/150‑1 (MQ=255)
                                           cAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCa                                                                                                            <  1:2639652/150‑1 (MQ=255)
                                               aTGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCa                                                                                                        <  2:227309/150‑1 (MQ=255)
                                               aTGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCa                                                                                                        <  2:1373661/150‑1 (MQ=255)
                                               aTGTTCATTCGCACCTCCTACAATTCCCGACGACACCAGCGGGATGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCa                                                                                                        >  2:1094679/1‑150 (MQ=255)
                                                tGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCAc                                                                                                       <  2:1496998/150‑1 (MQ=255)
                                                tGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCAc                                                                                                       <  1:2375589/150‑1 (MQ=255)
                                                tGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTAGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCAc                                                                                                       >  1:3582606/1‑150 (MQ=255)
                                                 gTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTAGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACg                                                                                                      <  1:1922036/150‑1 (MQ=255)
                                                    cATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGgca                                                                                                   >  2:893466/1‑150 (MQ=255)
                                                    cATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGgca                                                                                                   >  2:1037355/1‑150 (MQ=255)
                                                         gCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTAGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAgg                                                                                              >  1:2030319/1‑150 (MQ=255)
                                                          cACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGc                                                                                             >  2:3672959/1‑150 (MQ=255)
                                                                   cATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTa                                                                                    >  2:3099872/1‑150 (MQ=255)
                                                                    aTTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTAc                                                                                   <  1:158622/150‑1 (MQ=255)
                                                                    aTTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTAc                                                                                   >  2:740358/1‑150 (MQ=255)
                                                                     tttCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTAcc                                                                                  <  1:44089/150‑1 (MQ=255)
                                                                     tttCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTAcc                                                                                  >  1:1234506/1‑150 (MQ=255)
                                                                      ttCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTAccg                                                                                 <  1:3109129/150‑1 (MQ=255)
                                                                          cgccgcCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACcgccgc                                                                             <  1:943878/150‑1 (MQ=255)
                                                                           gccgccACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCcgcg                                                                            >  1:3268493/1‑150 (MQ=255)
                                                                              gccACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCa                                                                         <  1:650314/150‑1 (MQ=255)
                                                                               ccaccaGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCaa                                                                        >  1:4038194/1‑150 (MQ=255)
                                                                               ccaccaGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCaa                                                                        >  1:127621/1‑150 (MQ=255)
                                                                                 accaGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGt                                                                      <  1:288456/150‑1 (MQ=255)
                                                                                 accaGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGt                                                                      <  1:410214/150‑1 (MQ=255)
                                                                                   caGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCg                                                                    >  2:2747307/1‑150 (MQ=255)
                                                                                          aGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATg                                                             >  1:1219803/1‑150 (MQ=255)
                                                                                             gCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCa                                                          <  2:3526198/150‑1 (MQ=255)
                                                                                               tttAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGt                                                        >  1:3249814/1‑150 (MQ=255)
                                                                                                ttAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTg                                                       <  1:2412617/150‑1 (MQ=255)
                                                                                                ttAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTg                                                       <  1:567522/150‑1 (MQ=255)
                                                                                                ttAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTg                                                       <  1:2227154/150‑1 (MQ=255)
                                                                                                 tAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTgg                                                      >  2:450336/1‑150 (MQ=255)
                                                                                                  aGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTAGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGc                                                     >  2:1193036/1‑150 (MQ=255)
                                                                                                   gTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCa                                                    <  1:3173202/150‑1 (MQ=255)
                                                                                                          gTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGa                                             <  2:2596597/150‑1 (MQ=255)
                                                                                                          gTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGa                                             <  2:1649127/150‑1 (MQ=255)
                                                                                                           tGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGAc                                            <  1:230037/150‑1 (MQ=255)
                                                                                                            gCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACg                                           >  1:3566382/1‑150 (MQ=255)
                                                                                                              ccATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGcc                                         <  1:259450/150‑1 (MQ=255)
                                                                                                               cATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGccc                                        <  1:1311898/150‑1 (MQ=255)
                                                                                                               cATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGccc                                        <  1:3986990/150‑1 (MQ=255)
                                                                                                                aTAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCa                                       <  2:2381388/150‑1 (MQ=255)
                                                                                                                aTAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCa                                       <  2:2170285/150‑1 (MQ=255)
                                                                                                                      aGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCagcgagc                                 >  2:2738978/1‑150 (MQ=255)
                                                                                                                      aGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCagcgagc                                 <  2:2963422/150‑1 (MQ=255)
                                                                                                                          cacaTCTAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCTCCAGCACCCACGGCAGCAGGACAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCTGCGAGCGAAt                             <  2:550557/150‑1 (MQ=255)
                                                                                                                          cacaACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAAt                             <  1:2556571/150‑1 (MQ=255)
                                                                                                                          cacaACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAAt                             >  2:2323903/1‑150 (MQ=255)
                                                                                                                              acaaAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCa                         >  1:556110/1‑150 (MQ=255)
                                                                                                                                 aaaCCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTa                      <  1:2206085/150‑1 (MQ=255)
                                                                                                                                  aaccaaccAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAg                     >  2:4087692/1‑150 (MQ=255)
                                                                                                                                     caaccaacAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGaa                  <  1:1836844/150‑1 (MQ=255)
                                                                                                                                      aaccaacAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGaaa                 >  2:3042429/1‑150 (MQ=255)
                                                                                                                                       accaacAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGaaaa                <  2:962968/150‑1 (MQ=255)
                                                                                                                                         caacaaCACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACt              >  1:1598399/1‑150 (MQ=255)
                                                                                                                                         caacaaCACTGTAGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACt              >  1:3305485/1‑150 (MQ=255)
                                                                                                                                           acaacaCTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACttt            <  2:320923/150‑1 (MQ=255)
                                                                                                                                           acaacaCTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACttt            <  1:3700622/150‑1 (MQ=255)
                                                                                                                                            caacaCTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTc           >  2:3768111/1‑150 (MQ=255)
                                                                                                                                                acTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCa       >  1:1751245/1‑150 (MQ=255)
                                                                                                                                                acTGTAGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCa       >  2:3333131/1‑150 (MQ=255)
                                                                                                                                                 cTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAg      <  1:2339920/150‑1 (MQ=255)
                                                                                                                                                 cTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAg      <  1:2295285/150‑1 (MQ=255)
                                                                                                                                                  tGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAgg     >  2:3970206/1‑150 (MQ=255)
                                                                                                                                                  tGTAGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGACAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAgg     <  2:52768/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTCCGCCAGCATTCCGGCATCCGGTTCGGCTTTAAACGCTAACAGAATGTTCATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAA  >  CP009273/2889860‑2890158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑