Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,895,997 C→A 32.0% A248E (GCG→GAG)  ygcE → putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,895,9970CA32.0% 467.0 / 223.7 253A248E (GCG→GAG) ygcEputative kinase
Reads supporting (aligned to +/- strand):  ref base C (78/94);  new base A (37/44);  total (115/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 254 total aligned reads displayed.

GGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGGCGCAGGATCTTTCACCGGA  >  CP009273/2895848‑2896144
                                                                                                                                                     |                                                                                                                                                   
ggCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGc                                                                                                                                                     >  2:780685/1‑150 (MQ=255)
    ggACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTAc                                                                                                                                                 <  1:407664/150‑1 (MQ=255)
     gACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACt                                                                                                                                                <  1:2061967/150‑1 (MQ=255)
     gACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACt                                                                                                                                                >  1:1630005/1‑150 (MQ=255)
     gACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACt                                                                                                                                                <  1:3728452/150‑1 (MQ=255)
      aCTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTg                                                                                                                                               <  2:919939/150‑1 (MQ=255)
       cTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTgg                                                                                                                                              <  1:1796643/150‑1 (MQ=255)
        tGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGc                                                                                                                                             <  2:1839241/150‑1 (MQ=255)
           cGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGa                                                                                                                                          >  2:1032087/1‑150 (MQ=255)
           cGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGa                                                                                                                                          >  1:1423738/1‑150 (MQ=255)
            ggTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGAt                                                                                                                                         <  2:3984741/150‑1 (MQ=255)
            ggTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGAt                                                                                                                                         <  2:3148286/150‑1 (MQ=255)
              ttgtttgtACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTa                                                                                                                                       <  1:416999/150‑1 (MQ=255)
              ttgtttgtACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATNa                                                                                                                                       <  2:2584628/150‑1 (MQ=255)
               tgtttgtACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTAt                                                                                                                                      <  1:3071887/150‑1 (MQ=255)
                gtttgtACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATg                                                                                                                                     <  1:2669707/150‑1 (MQ=255)
                gtttgtACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATg                                                                                                                                     >  1:2341803/1‑150 (MQ=255)
                   tgtACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGtct                                                                                                                                  >  1:2185272/1‑150 (MQ=255)
                    gtACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGtctt                                                                                                                                 <  2:1147669/150‑1 (MQ=255)
                         accaGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTAtt                                                                                                                            <  1:3247864/150‑1 (MQ=255)
                           caGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTcc                                                                                                                          <  1:3923620/150‑1 (MQ=255)
                            aGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCg                                                                                                                         <  2:3673231/150‑1 (MQ=255)
                              tGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCa                                                                                                                       <  2:4171034/150‑1 (MQ=255)
                                aTAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCaaa                                                                                                                     >  1:3216558/1‑150 (MQ=255)
                                 tAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAAc                                                                                                                    <  1:1997178/150‑1 (MQ=255)
                                 tAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAAc                                                                                                                    >  1:3604613/1‑150 (MQ=255)
                                  aaaCCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACa                                                                                                                   <  2:628284/150‑1 (MQ=255)
                                   aaCCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACAt                                                                                                                  <  1:525521/150‑1 (MQ=255)
                                   aaCCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACAt                                                                                                                  <  2:2937107/150‑1 (MQ=255)
                                    aCCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACAtt                                                                                                                 >  2:1259323/1‑150 (MQ=255)
                                       ggTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCt                                                                                                              <  1:1717671/150‑1 (MQ=255)
                                          aGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAAATTCTCTTGGCACTTACATCGCAATGATGATGAAAGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCATCTATTCCGCAAACATTGCTgga                                                                                                           >  2:2692602/1‑148 (MQ=255)
                                            aaGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATg                                                                                                         >  1:1003477/1‑150 (MQ=255)
                                            aaGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATg                                                                                                         <  2:2618325/150‑1 (MQ=255)
                                            aaGCTCGGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATg                                                                                                         <  2:3160534/150‑1 (MQ=255)
                                             aGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGa                                                                                                        >  1:212077/1‑150 (MQ=255)
                                              gCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGaa                                                                                                       <  1:396572/150‑1 (MQ=255)
                                                 ctGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGt                                                                                                    <  1:3405634/150‑1 (MQ=255)
                                                    ggggCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTAc                                                                                                 <  2:1525190/150‑1 (MQ=255)
                                                       gCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGt                                                                                              <  1:2610917/150‑1 (MQ=255)
                                                       gCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGt                                                                                              <  1:2245253/150‑1 (MQ=255)
                                                         cGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTAt                                                                                            >  1:3188023/1‑150 (MQ=255)
                                                         cGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTAt                                                                                            <  2:3713796/150‑1 (MQ=255)
                                                         cGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTAt                                                                                            >  1:1208484/1‑150 (MQ=255)
                                                           gATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTc                                                                                          <  2:4003085/150‑1 (MQ=255)
                                                           gATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTc                                                                                          <  1:3125832/150‑1 (MQ=255)
                                                           gATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTc                                                                                          >  2:3357239/1‑150 (MQ=255)
                                                            aTTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCg                                                                                         >  2:414565/1‑150 (MQ=255)
                                                              tACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCa                                                                                       >  2:1642848/1‑150 (MQ=255)
                                                                     tgatgaAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCAt                                                                                >  2:459078/1‑150 (MQ=255)
                                                                      gatgaAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCAtg                                                                               <  1:2478258/150‑1 (MQ=255)
                                                                        tgaAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCAtgtg                                                                             <  2:2806044/150‑1 (MQ=255)
                                                                        tgaAAAGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATTATTAACGGCAAAGCACTGCCGAAAGATCCAGTATCGTACTGGCCGATTATGTCTTCTATTACGCAAACATTGCTTTATGAATGTTACGGTATTCGCAAAGGCAtgtg                                                                             <  1:1128606/150‑1 (MQ=255)
                                                                          aaaCGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGa                                                                           >  1:1905405/1‑150 (MQ=255)
                                                                             cggcggTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACgg                                                                        >  2:3877578/1‑150 (MQ=255)
                                                                                cggTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGa                                                                     <  2:4046534/150‑1 (MQ=255)
                                                                                    aaTTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAgctg                                                                 >  1:3765779/1‑150 (MQ=255)
                                                                                    aaTTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAgctg                                                                 <  1:1789148/150‑1 (MQ=255)
                                                                                     aTTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAgctgg                                                                >  1:4125716/1‑150 (MQ=255)
                                                                                       ttCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAgctggct                                                              >  2:1796748/1‑150 (MQ=255)
                                                                                       ttCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAgctggct                                                              >  1:2216011/1‑150 (MQ=255)
                                                                                           ctTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTgcgc                                                          >  2:3257228/1‑150 (MQ=255)
                                                                                             tGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGa                                                        >  2:935899/1‑150 (MQ=255)
                                                                                                         cGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGa                                            >  1:1078047/1‑150 (MQ=255)
                                                                                                          gCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAg                                           <  1:636544/150‑1 (MQ=255)
                                                                                                          gCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAg                                           <  1:2821327/150‑1 (MQ=255)
                                                                                                            aTTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTc                                         <  2:3899209/150‑1 (MQ=255)
                                                                                                            aTTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTAATCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAATGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTc                                         >  2:1018578/1‑150 (MQ=255)
                                                                                                              tgatgatgaACGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGt                                       >  1:3319134/1‑150 (MQ=255)
                                                                                                                atgatgaACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTa                                     <  1:2160357/150‑1 (MQ=255)
                                                                                                                atgatgaACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTa                                     <  2:3526265/150‑1 (MQ=255)
                                                                                                                atgatgaACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTa                                     <  2:3526277/150‑1 (MQ=255)
                                                                                                                  gatgaACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAAt                                   <  2:3071940/150‑1 (MQ=255)
                                                                                                                   atgaACGGCAAAGCACTGCCGAAAGATCCATGAGCGTACTGGCCGATTATGTCTTCTATTCCGCTAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGTGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAAtt                                  <  2:575038/150‑1 (MQ=255)
                                                                                                                        cGGCAAAGCACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGa                             >  2:1365773/1‑150 (MQ=255)
                                                                                                                            aaaGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGcc                         <  1:851336/150‑1 (MQ=255)
                                                                                                                             aaGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCa                        <  2:1965343/150‑1 (MQ=255)
                                                                                                                             aaGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCa                        >  2:3013413/1‑150 (MQ=255)
                                                                                                                             aaGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCa                        <  1:157697/150‑1 (MQ=255)
                                                                                                                               gCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAgg                      <  1:780685/150‑1 (MQ=255)
                                                                                                                                cACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAggg                     <  1:1606846/150‑1 (MQ=255)
                                                                                                                                cACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAAGTCAGGATGCCAggg                     >  2:86793/1‑150 (MQ=255)
                                                                                                                                cACTGCCGAAAGATCCAGTAGCGTACTGGCAGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAggg                     <  1:521058/150‑1 (MQ=255)
                                                                                                                                cACTGCCGAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAggg                     >  1:2950596/1‑150 (MQ=255)
                                                                                                                                 aCTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGgc                    >  2:2165899/1‑150 (MQ=255)
                                                                                                                                 aCTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGgc                    >  2:728017/1‑150 (MQ=255)
                                                                                                                                   tGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGgcgc                  >  1:3501764/1‑150 (MQ=255)
                                                                                                                                    gCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGGCGCa                 >  1:1261474/1‑150 (MQ=255)
                                                                                                                                    gCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGGCGCa                 <  2:3389237/150‑1 (MQ=255)
                                                                                                                                      ataaaGATCAATTAGCGTACTGTCCGATTATGTCTTAAATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTAGCAAAGGCATGTGGAAGGTGATCTTTCTGCGCGACATGCTAGGCGATTCGTTAATTAAGGATGCCAGGGCGCAgg               >  2:744082/3‑150 (MQ=255)
                                                                                                                                       gAAAGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGGCGCAGGa              <  2:2523305/150‑1 (MQ=255)
                                                                                                                                         aaGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGGCGCAGGATc            <  2:1920890/150‑1 (MQ=255)
                                                                                                                                          aGATCCAGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGGCGCAGGATCt           >  2:3971974/1‑150 (MQ=255)
                                                                                                                                           gATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGGCGCAGGATCtt          <  1:1142681/150‑1 (MQ=255)
                                                                                                                                           gATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGGCGCAGGATCtt          >  1:3177787/1‑150 (MQ=255)
                                                                                                                                            aTCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGGCGCAGGATCttt         <  1:3251009/150‑1 (MQ=255)
                                                                                                                                            aTCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGGCGCAGGATCttt         >  2:1095337/1‑150 (MQ=255)
                                                                                                                                              ccAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGGCGCAGGATCTTTCa       >  1:3475911/1‑150 (MQ=255)
                                                                                                                                              ccAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGGCGCAGGATCTTTCa       >  1:4100893/1‑150 (MQ=255)
                                                                                                                                                aGTAGAGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGGCGCAGGATCTTTCAcc     <  2:970507/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTGCTGTATGAAGGTTACGGTATTCGCAAAGGCATGTGGACGGTGAGCTGGCTGCGCGACATGCTAGGCGAGTCGTTAATTCAGGATGCCAGGGCGCAGGATCTTTCACCGGA  >  CP009273/2895848‑2896144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑