Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,931,717 C→A 100% N307K (AAC→AAA)  fucK → L‑fuculokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,931,7170CA100.0% 827.7 / NA 237N307K (AAC→AAA) fucKL‑fuculokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (105/132);  total (105/132)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 242 total aligned reads displayed.

TGGCGCTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATT  >  CP009273/2931570‑2931863
                                                                                                                                                   |                                                                                                                                                  
tGGCGCTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAacc                                                                                                                                                  <  1:1914232/150‑1 (MQ=255)
tGGCGCTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAacc                                                                                                                                                  >  2:2091768/1‑150 (MQ=255)
 ggCGCTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCa                                                                                                                                                 >  1:1358602/1‑150 (MQ=255)
  gcgcTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAg                                                                                                                                                >  1:3866177/1‑150 (MQ=255)
   cgcTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAgg                                                                                                                                               <  2:2415896/150‑1 (MQ=255)
   cgcTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAgg                                                                                                                                               >  2:3618485/1‑150 (MQ=255)
    gcTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGt                                                                                                                                              <  1:388418/150‑1 (MQ=255)
      tGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTAt                                                                                                                                            >  1:1420880/1‑150 (MQ=255)
       ggTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATg                                                                                                                                           >  2:3957328/1‑150 (MQ=255)
         tGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCa                                                                                                                                         >  1:454172/1‑150 (MQ=255)
            tGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAAtg                                                                                                                                      >  1:3947258/1‑150 (MQ=255)
             gAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAAtgg                                                                                                                                     >  1:3271199/1‑150 (MQ=255)
              aaCAAAATGAACCCGTGCTCTCTTCCGGTACAGGGGAAATTTTAATGGTTCGCAGCGCCGAGGGTGATCCTTCGCTGGTCAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTTTAAACCAGGTATGCAAtggc                                                                                                                                    >  2:3072160/1‑150 (MQ=255)
                     tGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATc                                                                                                                             >  1:3771278/1‑150 (MQ=255)
                        aCCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCgg                                                                                                                          <  2:4077404/150‑1 (MQ=255)
                            gTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATGCTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTg                                                                                                                      >  1:116940/1‑150 (MQ=255)
                            gTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTg                                                                                                                      <  2:512628/150‑1 (MQ=255)
                             tGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGc                                                                                                                     <  2:3810196/150‑1 (MQ=255)
                                tctctTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTgg                                                                                                                  >  2:2452215/1‑150 (MQ=255)
                                 ctctTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGa                                                                                                                 >  1:641894/1‑150 (MQ=255)
                                   ctTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAAt                                                                                                               <  2:669463/150‑1 (MQ=255)
                                    ttCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATg                                                                                                              <  1:3384604/150‑1 (MQ=255)
                                    ttCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATg                                                                                                              <  2:1557373/150‑1 (MQ=255)
                                     tCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATgg                                                                                                             >  1:3199728/1‑150 (MQ=255)
                                      ccGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATggg                                                                                                            <  1:3707479/150‑1 (MQ=255)
                                        ggTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTg                                                                                                          >  2:1865286/1‑150 (MQ=255)
                                        ggTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTg                                                                                                          >  2:2555204/1‑150 (MQ=255)
                                        ggTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTg                                                                                                          >  2:3691384/1‑150 (MQ=255)
                                          tACATGGGAAATTTTAATGGTTCGCAGCGCACAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTgag                                                                                                        >  1:3851184/1‑150 (MQ=255)
                                           aCATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTgaga                                                                                                       <  2:1028428/150‑1 (MQ=255)
                                            cATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGaa                                                                                                      <  2:2999482/150‑1 (MQ=255)
                                             aTGGGAAATTTTAATGGGTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGaaa                                                                                                     <  2:63494/150‑1 (MQ=255)
                                              tGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGaaaa                                                                                                    >  2:3629519/1‑150 (MQ=255)
                                                   aaTTTTAATGGTTCGCAGCGCCCCGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGCTTGTATAAACCAGGTATGCACTGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGtt                                                                                               >  2:545821/1‑150 (MQ=255)
                                                    aTTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTc                                                                                              >  1:3914006/1‑150 (MQ=255)
                                                    aTTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTc                                                                                              >  1:489469/1‑150 (MQ=255)
                                                        tAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGa                                                                                          >  2:3980865/1‑150 (MQ=255)
                                                        tAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGa                                                                                          >  2:1449284/1‑150 (MQ=255)
                                                         aaTGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGAc                                                                                         >  1:2934958/1‑150 (MQ=255)
                                                         aaTGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGAc                                                                                         <  2:244221/150‑1 (MQ=255)
                                                             gTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCt                                                                                     <  2:2834645/150‑1 (MQ=255)
                                                              ttCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTg                                                                                    <  1:424868/150‑1 (MQ=255)
                                                              ttCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTg                                                                                    <  1:777998/150‑1 (MQ=255)
                                                               tCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGa                                                                                   <  1:2009519/150‑1 (MQ=255)
                                                                  cAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAac                                                                                >  2:1633136/1‑150 (MQ=255)
                                                                  cAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAac                                                                                <  1:1679922/150‑1 (MQ=255)
                                                                    gcgcCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAacac                                                                              <  1:4001281/150‑1 (MQ=255)
                                                                     cgcCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACAcc                                                                             >  2:722024/1‑150 (MQ=255)
                                                                      gcCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACAccc                                                                            >  2:2317530/1‑150 (MQ=255)
                                                                      gcCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACAccc                                                                            <  2:3286252/150‑1 (MQ=255)
                                                                       cccAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCt                                                                           >  1:1102530/1‑150 (MQ=255)
                                                                       cccAGGTTGATACTTCGCTGTTAAGTCAGTACGCAGGTTCCACCTGCGATCTGGATTGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAACCTGTTCTGGTCGGCTGAAACACCCt                                                                           <  2:1708404/150‑1 (MQ=255)
                                                                         cAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTgg                                                                         <  2:921615/150‑1 (MQ=255)
                                                                         cAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTgg                                                                         >  1:3132281/1‑150 (MQ=255)
                                                                           ggTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCa                                                                       <  2:3394404/150‑1 (MQ=255)
                                                                              tGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAAt                                                                    >  1:1284874/1‑150 (MQ=255)
                                                                               gATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATg                                                                   >  2:429086/1‑150 (MQ=255)
                                                                                 tACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGtt                                                                 >  2:1243708/1‑150 (MQ=255)
                                                                                  aCTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGttg                                                                >  2:1478221/1‑150 (MQ=255)
                                                                                       gCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGttgattga                                                           <  1:2147923/150‑1 (MQ=255)
                                                                                        cTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTgaa                                                          <  2:370413/150‑1 (MQ=255)
                                                                                        cTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTgaa                                                          >  2:4048105/1‑150 (MQ=255)
                                                                                        cTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTgaa                                                          >  2:2185701/1‑150 (MQ=255)
                                                                                         tGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTgaag                                                         <  1:2468665/150‑1 (MQ=255)
                                                                                            tAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCTAATGTTGATTGaagaag                                                      >  2:1933778/1‑150 (MQ=255)
                                                                                             aaGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGc                                                     <  2:133276/150‑1 (MQ=255)
                                                                                               gTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTc                                                   <  2:2712064/150‑1 (MQ=255)
                                                                                               gTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTc                                                   <  1:3481290/150‑1 (MQ=255)
                                                                                               gTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTc                                                   <  1:243341/150‑1 (MQ=255)
                                                                                                tCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCg                                                  <  1:3824382/150‑1 (MQ=255)
                                                                                                 cAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGt                                                 <  2:2617096/150‑1 (MQ=255)
                                                                                                 cAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGt                                                 >  1:1003355/1‑150 (MQ=255)
                                                                                                  aGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTc                                                >  1:3630398/1‑150 (MQ=255)
                                                                                                  aGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTc                                                >  1:2587817/1‑150 (MQ=255)
                                                                                                   gTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCt                                               <  1:2990890/150‑1 (MQ=255)
                                                                                                      cGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGAt                                            <  2:882497/150‑1 (MQ=255)
                                                                                                       gCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATc                                           >  1:98725/1‑150 (MQ=255)
                                                                                                       gCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATACAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATc                                           <  2:3990983/150‑1 (MQ=255)
                                                                                                           gTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCgcgc                                       >  2:459578/1‑150 (MQ=255)
                                                                                                             tCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCt                                     <  1:2378445/150‑1 (MQ=255)
                                                                                                             tCCACCTGCGAACTGGAGAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCt                                     <  2:2860330/150‑1 (MQ=255)
                                                                                                                  cTGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGgcgc                                >  2:323679/1‑150 (MQ=255)
                                                                                                                   tGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGcgcg                               <  2:2471258/150‑1 (MQ=255)
                                                                                                                   tGCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGcgcg                               <  2:272228/150‑1 (MQ=255)
                                                                                                                     cGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGa                             <  2:3222873/150‑1 (MQ=255)
                                                                                                                       aaCTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATg                           >  2:3501876/1‑150 (MQ=255)
                                                                                                                       aaCTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATg                           <  1:3604708/150‑1 (MQ=255)
                                                                                                                       aaCTGGATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATg                           >  1:2810469/1‑150 (MQ=255)
                                                                                                                           ggATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGt                       >  2:934487/1‑150 (MQ=255)
                                                                                                                            gATAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTa                      >  1:608970/1‑150 (MQ=255)
                                                                                                                             aTAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTaa                     >  1:227009/1‑150 (MQ=255)
                                                                                                                             aTAGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTaa                     >  2:751134/1‑150 (MQ=255)
                                                                                                                               aGCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTaaaa                   <  2:2779216/150‑1 (MQ=255)
                                                                                                                                gCCAGGCAGGGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTaaaaa                  >  2:1922549/1‑150 (MQ=255)
                                                                                                                                    ggcaggGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCa              <  1:2014526/150‑1 (MQ=255)
                                                                                                                                    ggcaggGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCa              <  1:2996762/150‑1 (MQ=255)
                                                                                                                                      caggGTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAgt            <  1:3088255/150‑1 (MQ=255)
                                                                                                                                         ggTTGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGtgtg         >  2:2107802/1‑150 (MQ=255)
                                                                                                                                            tGTATAAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGAtt      <  2:204029/150‑1 (MQ=255)
                                                                                                                                                taAACCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTAtt  <  1:3222409/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGGCGCTGGTGCTGAACAAAATGAACCCGTGCTCTCTTCCGGTACATGGGAAATTTTAATGGTTCGCAGCGCCCAGGTTGATACTTCGCTGTTAAGTCAGTACGCCGGTTCCACCTGCGAACTGGATAGCCAGGCAGGGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATT  >  CP009273/2931570‑2931863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑