BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,983,700	T→G	7.8%	Q7P (CAG→CCG)	yqeK ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,983,700	0	T	G	7.8%	712.2 / 50.2	239	Q7P (CAG→CCG)	yqeK	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (109/111); new base G (14/5); total (123/116)
Fisher's exact test for biased strand distribution p-value = 5.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

AATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAGTCATTCTACGTAACGTCTCCATAAGGTGA  >  CP009273/2983551‑2983849

aaTTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCt                                                                                                                                                       >  2:1706844/1‑150 (MQ=255)

     aCATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGaa                                                                                                                                                  <  1:4107245/150‑1 (MQ=255)

        ttCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAAt                                                                                                                                               >  2:2965457/1‑150 (MQ=255)

          cacaTATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTc                                                                                                                                             <  2:833803/150‑1 (MQ=255)

              tatCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATg                                                                                                                                         <  2:1421783/150‑1 (MQ=255)

              tatCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCGGCGAAAATTCAATg                                                                                                                                         >  2:2146854/1‑150 (MQ=255)

                tCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTc                                                                                                                                       <  2:1118393/150‑1 (MQ=255)

                      aTTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGt                                                                                                                                 <  2:2663402/150‑1 (MQ=255)

                       tttAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGta                                                                                                                                <  1:1841844/150‑1 (MQ=255)

                         ttaTGTACATGTTGTTGTTTGCGTGAGAACATTCTATGCATTAAGGTTTTTTTTTTACGGAATCCTTGGTTTCTTTTTAGTGTCCGAATTTACTTTATCAGGCATATTTACCATTTCTTGAATCTGCGTAAATTCTTTGTCCTTTGtata                                                                                                                              <  2:2757406/148‑1 (MQ=255)

                           aTGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATAcc                                                                                                                            >  1:3095584/1‑150 (MQ=255)

                            tGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCt                                                                                                                           <  1:23881/150‑1 (MQ=255)

                             gTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTc                                                                                                                          <  2:3169972/150‑1 (MQ=255)

                                 atgatgTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACAt                                                                                                                      <  2:3670320/150‑1 (MQ=255)

                                  tgatgTTGTTTGCGTGAGAACATTCTCAGCATTAAGGACTTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACAtt                                                                                                                     >  2:1313036/1‑150 (MQ=255)

                                  tgatgTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACAtt                                                                                                                     >  2:21490/1‑150 (MQ=255)

                                  tgatgTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACAtt                                                                                                                     >  2:1198659/1‑150 (MQ=255)

                                  tgatgTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACAtt                                                                                                                     <  2:1405137/150‑1 (MQ=255)

                                     tgttgtTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACAttttt                                                                                                                  <  1:513609/150‑1 (MQ=255)

                                     tgttgtTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCGGCGAAAATTCAATGTCCATTGTATACCTCACAttttt                                                                                                                  >  1:3167925/1‑150 (MQ=255)

                                       ttgttTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTAc                                                                                                                <  2:3141546/150‑1 (MQ=255)

                                       ttgttTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCGGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTAc                                                                                                                >  2:3475047/1‑150 (MQ=255)

                                         gttTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCg                                                                                                              >  1:2261905/1‑150 (MQ=255)

                                          tttGCGTGAGAACATTCTCAGCATTAAGTAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGt                                                                                                             <  2:3756652/150‑1 (MQ=255)

                                              cGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACt                                                                                                         <  2:4073540/150‑1 (MQ=255)

                                               gTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGACAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTc                                                                                                        >  1:856945/1‑150 (MQ=255)

                                               gTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTc                                                                                                        >  2:2841511/1‑150 (MQ=255)

                                               gTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTc                                                                                                        <  1:1613540/150‑1 (MQ=255)

                                               gTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTc                                                                                                        >  1:4115657/1‑150 (MQ=255)

                                               gTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTc                                                                                                        <  2:4100211/150‑1 (MQ=255)

                                                 gagaACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGa                                                                                                      >  1:2608057/1‑150 (MQ=255)

                                                   gaACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATg                                                                                                    >  1:2290916/1‑150 (MQ=255)

                                                   gaACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATg                                                                                                    <  1:1655670/150‑1 (MQ=255)

                                                    aaCATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGt                                                                                                   >  2:4000407/1‑150 (MQ=255)

                                                    aaCATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGt                                                                                                   <  1:673734/150‑1 (MQ=255)

                                                       aTTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCTAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTAc                                                                                                >  1:624014/1‑150 (MQ=255)

                                                        ttCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACt                                                                                               <  1:1721832/150‑1 (MQ=255)

                                                        ttCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACt                                                                                               <  1:1695183/150‑1 (MQ=255)

                                                          ctcAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGt                                                                                             >  2:557343/1‑150 (MQ=255)

                                                           tcAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGtt                                                                                            >  1:292859/1‑150 (MQ=255)

                                                           tcAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGtt                                                                                            <  2:2306992/150‑1 (MQ=255)

                                                           tcAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGtt                                                                                            <  2:3731358/150‑1 (MQ=255)

                                                            cAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTc                                                                                           <  1:809164/150‑1 (MQ=255)

                                                               cATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCaat                                                                                        >  1:414503/1‑150 (MQ=255)

                                                                aTTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCaata                                                                                       >  2:892708/1‑150 (MQ=255)

                                                                aTTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCaata                                                                                       >  1:3624755/1‑150 (MQ=255)

                                                                 ttAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCaataa                                                                                      >  2:2466497/1‑150 (MQ=255)

                                                                  tAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCaataat                                                                                     <  1:3500040/150‑1 (MQ=255)

                                                                   aaGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATc                                                                                    >  1:1340472/1‑150 (MQ=255)

                                                                     ggAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCAc                                                                                  <  2:1448144/150‑1 (MQ=255)

                                                                      gAATTTTTTTAACGGAATCCGTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCAcc                                                                                 >  1:2570669/1‑150 (MQ=255)

                                                                        aTTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCtt                                                                               <  1:2425023/150‑1 (MQ=255)

                                                                          ttttttAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTcc                                                                             <  1:944350/150‑1 (MQ=255)

                                                                            ttttAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCAt                                                                           <  1:2118283/150‑1 (MQ=255)

                                                                            ttttAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCAt                                                                           >  1:3001367/1‑150 (MQ=255)

                                                                            ttttAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCAt                                                                           >  1:148700/1‑150 (MQ=255)

                                                                            ttttAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCAt                                                                           <  2:2043889/150‑1 (MQ=255)

                                                                               tAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCaa                                                                        >  2:1704076/1‑150 (MQ=255)

                                                                                aaCGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAAt                                                                       >  1:4130093/1‑150 (MQ=255)

                                                                                  cGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATAc                                                                     >  1:105999/1‑150 (MQ=255)

                                                                                   ggAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACt                                                                    >  1:2201804/1‑150 (MQ=255)

                                                                                    gAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTa                                                                   >  2:4039133/1‑150 (MQ=255)

                                                                                     aaTCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTaa                                                                  >  2:3123793/1‑150 (MQ=255)

                                                                                     aaTCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTaa                                                                  >  2:3602778/1‑150 (MQ=255)

                                                                                        ccTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAAt                                                               >  2:140685/1‑150 (MQ=255)

                                                                                         cTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAAtt                                                              <  2:3505864/150‑1 (MQ=255)

                                                                                         cTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTACATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAAtt                                                              <  1:2369778/150‑1 (MQ=255)

                                                                                           tGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCGGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTaa                                                            >  2:565851/1‑150 (MQ=255)

                                                                                            ggTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAAt                                                           <  1:714966/150‑1 (MQ=255)

                                                                                              tttCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATAc                                                         >  2:1916014/1‑150 (MQ=255)

                                                                                              tttCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCGGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATAc                                                         >  1:3273715/1‑150 (MQ=255)

                                                                                               ttCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATAcc                                                        >  1:3754987/1‑150 (MQ=255)

                                                                                                     ttCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAAt                                                  <  2:1972348/150‑1 (MQ=255)

                                                                                                         aGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGc                                              >  2:1623174/1‑150 (MQ=255)

                                                                                                         aGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCGGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGc                                              >  1:1149009/1‑150 (MQ=255)

                                                                                                          gTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGcc                                             >  2:1573226/1‑150 (MQ=255)

                                                                                                            ccGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGa                                           >  1:2791320/1‑150 (MQ=255)

                                                                                                             cGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGAt                                          >  2:196053/1‑150 (MQ=255)

                                                                                                              gAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATa                                         >  2:3866757/1‑150 (MQ=255)

                                                                                                              gAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATa                                         >  1:1004014/1‑150 (MQ=255)

                                                                                                              gAATTTACAATATCATGCATATAGACCATTTCATGAATCGGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATa                                         <  1:2146931/150‑1 (MQ=255)

                                                                                                               aaTTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATaa                                        <  1:1523287/150‑1 (MQ=255)

                                                                                                                aTTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAAc                                       <  2:264504/150‑1 (MQ=255)

                                                                                                                  ttACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACaa                                     >  1:3868201/1‑150 (MQ=255)

                                                                                                                    aCAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAat                                   <  1:3315064/150‑1 (MQ=255)

                                                                                                                     cAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAata                                  >  1:203907/1‑150 (MQ=255)

                                                                                                                       atatCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAAtata                                <  2:3670148/150‑1 (MQ=255)

                                                                                                                        tatCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAg                               <  2:1218426/150‑1 (MQ=255)

                                                                                                                            aTGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAGTCAt                           <  1:2626445/150‑1 (MQ=255)

                                                                                                                            aTGAATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAGTCAt                           <  1:3074559/150‑1 (MQ=255)

                                                                                                                             tGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAGTCAtt                          <  1:3950876/150‑1 (MQ=255)

                                                                                                                             tGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAGTCAtt                          >  2:281753/1‑150 (MQ=255)

                                                                                                                              gCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAGTCATTc                         <  1:2303590/150‑1 (MQ=255)

                                                                                                                                 tataGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAGTCATTCTAc                      >  2:3285522/1‑150 (MQ=255)

                                                                                                                                         aTTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAGTCATTCTACGTAACGtc              >  2:3359446/1‑150 (MQ=255)

                                                                                                                                               tGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAGTCATTCTACGTAACGTCTCCATa        <  1:2788148/150‑1 (MQ=255)

                                                                                                                                                  aTCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAGTCATTCTACGTAACGTCTCCATAAgg     >  2:1843336/1‑150 (MQ=255)

                                                                                                                                                  aTCGGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAGTCATTCTACGTAACGTCTCCATAAgg     >  2:85026/1‑150 (MQ=255)

                                                                                                                                                    cTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAGTCATTCTACGTAACGTCTCCATAAGGTg   >  2:2958200/1‑150 (MQ=255)

                                                                                                                                                    cTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAGTCATTCTACGTAACGTCTCCATAAGGTg   >  2:869512/1‑150 (MQ=255)

AATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAATTAATACCCCTAATGTGCCGATAACAAATATAGTCATTCTACGTAACGTCTCCATAAGGTGA  >  CP009273/2983551‑2983849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`