Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,211,421 T→A 8.3% K339* (AAG→TAG)  aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,211,4210TA 8.3% 702.4 / 43.9 216K339* (AAG→TAG) aerfused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base T (119/79);  new base A (8/10);  total (127/89)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 223 total aligned reads displayed.

CACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCA  >  CP009273/3211272‑3211569
                                                                                                                                                     |                                                                                                                                                    
cacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCtt                                                                                                                                                      <  2:2016218/150‑1 (MQ=255)
cacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCtt                                                                                                                                                      >  2:3193307/1‑150 (MQ=255)
cacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCtt                                                                                                                                                      <  1:438661/150‑1 (MQ=255)
cacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCtt                                                                                                                                                      <  1:3796203/150‑1 (MQ=255)
  cTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGa                                                                                                                                                    >  2:236848/1‑150 (MQ=255)
       aaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCGGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCAcc                                                                                                                                               <  2:2641648/150‑1 (MQ=255)
         aCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGt                                                                                                                                             >  2:2085795/1‑150 (MQ=255)
            tgtGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGt                                                                                                                                          <  2:1890902/148‑1 (MQ=255)
                  cTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGc                                                                                                                                    >  1:2998855/1‑150 (MQ=255)
                   tGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGcc                                                                                                                                   <  1:146181/150‑1 (MQ=255)
                   tGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGcc                                                                                                                                   >  1:3476728/1‑150 (MQ=255)
                    gTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTAGATCACCGTGGTCATCGCCt                                                                                                                                  >  1:1847313/1‑150 (MQ=255)
                     ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTc                                                                                                                                 >  1:1763294/1‑150 (MQ=255)
                      tCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTcc                                                                                                                                <  1:2530721/150‑1 (MQ=255)
                       cGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTccc                                                                                                                               >  1:1034920/1‑150 (MQ=255)
                       cGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTccc                                                                                                                               >  1:2440946/1‑150 (MQ=255)
                       cGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTccc                                                                                                                               >  1:1628585/1‑150 (MQ=255)
                         ccGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCa                                                                                                                             <  1:2908574/150‑1 (MQ=255)
                          cGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCAc                                                                                                                            <  2:1475439/150‑1 (MQ=255)
                              aCGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTg                                                                                                                        >  1:2709951/1‑150 (MQ=255)
                               cgcgCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGc                                                                                                                       >  2:2143457/1‑150 (MQ=255)
                                  gccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACt                                                                                                                    <  1:2781533/150‑1 (MQ=255)
                                  gccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACt                                                                                                                    <  1:195784/150‑1 (MQ=255)
                                      cTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCg                                                                                                                >  2:598256/1‑150 (MQ=255)
                                          cACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACTGTGGTCATCGCCTCCCCACCCTGCACTGCCGCAtt                                                                                                            >  2:2343685/1‑150 (MQ=255)
                                              gccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTg                                                                                                        <  2:1296294/150‑1 (MQ=255)
                                              gccgcAATCAGGGCCAGAATATTGTTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTg                                                                                                        <  2:3741142/150‑1 (MQ=255)
                                                cgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGc                                                                                                      >  2:401710/1‑150 (MQ=255)
                                                  cATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAg                                                                                                    >  1:1661232/1‑150 (MQ=255)
                                                  cATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAg                                                                                                    >  2:1257565/1‑150 (MQ=255)
                                                   aTTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGt                                                                                                   >  1:3095604/1‑150 (MQ=255)
                                                   aTTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGt                                                                                                   >  1:414162/1‑150 (MQ=255)
                                                      cAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGAt                                                                                                <  1:610775/150‑1 (MQ=255)
                                                        gggCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTg                                                                                              <  2:2328469/150‑1 (MQ=255)
                                                         ggCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGa                                                                                             >  2:1383766/1‑150 (MQ=255)
                                                           ccAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACa                                                                                           <  1:485020/150‑1 (MQ=255)
                                                             aGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGt                                                                                         <  2:1936271/150‑1 (MQ=255)
                                                                  atTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATc                                                                                    <  1:4133312/150‑1 (MQ=255)
                                                                      gTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTAGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCa                                                                                <  1:3152284/150‑1 (MQ=255)
                                                                       tCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAg                                                                               >  2:2575052/1‑150 (MQ=255)
                                                                       tCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAg                                                                               <  2:2552508/150‑1 (MQ=255)
                                                                        cTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGc                                                                              >  1:2337653/1‑150 (MQ=255)
                                                                         tGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGcc                                                                             >  2:31288/1‑150 (MQ=255)
                                                                          gAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCg                                                                            >  2:564840/1‑150 (MQ=255)
                                                                          gAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTAGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCg                                                                            <  2:1407665/150‑1 (MQ=255)
                                                                           aaaCGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGa                                                                           >  2:2198441/1‑150 (MQ=255)
                                                                           aaaCGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGa                                                                           <  2:2067444/150‑1 (MQ=255)
                                                                           aaaCGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGa                                                                           >  1:4123902/1‑150 (MQ=255)
                                                                           aaaCGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGa                                                                           >  1:3987194/1‑150 (MQ=255)
                                                                            aaCGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGAc                                                                          <  1:831212/150‑1 (MQ=255)
                                                                            aaCGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTAGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGAc                                                                          <  1:1209550/150‑1 (MQ=255)
                                                                            aaCGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTAGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGAc                                                                          <  1:1789308/150‑1 (MQ=255)
                                                                             aCGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACg                                                                         >  1:660475/1‑150 (MQ=255)
                                                                               gCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGcc                                                                       <  2:1236678/150‑1 (MQ=255)
                                                                                     tCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTAGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCa                                                                 <  2:2701572/150‑1 (MQ=255)
                                                                                      cGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTGGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCag                                                                >  1:3354451/1‑150 (MQ=255)
                                                                                      cGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCag                                                                >  2:573383/1‑150 (MQ=255)
                                                                                       gTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCaga                                                               <  2:2642858/150‑1 (MQ=255)
                                                                                        ttAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCAGTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCagag                                                              >  2:1738640/1‑150 (MQ=255)
                                                                                         tAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGt                                                             >  2:3966183/1‑150 (MQ=255)
                                                                                          aaTCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGtt                                                            >  1:1889212/1‑150 (MQ=255)
                                                                                          aaTCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGtt                                                            >  2:1653547/1‑150 (MQ=255)
                                                                                           aTCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTc                                                           >  1:1190572/1‑150 (MQ=255)
                                                                                            tCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCt                                                          >  2:2843083/1‑150 (MQ=255)
                                                                                             cAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTAGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTg                                                         >  1:1243985/1‑150 (MQ=255)
                                                                                                 gAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTc                                                     <  2:1748411/150‑1 (MQ=255)
                                                                                                 gAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTc                                                     >  1:3254277/1‑150 (MQ=255)
                                                                                                 gAAGTAATGGTCCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCACCACCCTGCACTGCCGCCTTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGAGGCAGAGTTCTGTTTc                                                     >  2:2471201/1‑150 (MQ=255)
                                                                                                   aGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCAc                                                   >  2:2252956/1‑150 (MQ=255)
                                                                                                     tAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCg                                                 >  1:3866485/1‑150 (MQ=255)
                                                                                                      aaTGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGa                                                >  2:2479040/1‑150 (MQ=255)
                                                                                                           tGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGAcgccg                                           <  1:2673179/150‑1 (MQ=255)
                                                                                                             ccAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACgccgcc                                         >  2:3601913/1‑150 (MQ=255)
                                                                                                              cAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCCGCGTTCTGTTTCACCGACgccgccc                                        >  2:3230011/1‑149 (MQ=255)
                                                                                                              cAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCa                                        >  1:960467/1‑150 (MQ=255)
                                                                                                              cAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGGTTATCGGCAGCCGACGCCGTGGCAGAGGTCTGGTTCACCGACGCCGCCa                                        >  2:4139896/1‑150 (MQ=255)
                                                                                                               aaTGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCAt                                       >  2:367075/1‑150 (MQ=255)
                                                                                                                 tGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCAACGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCAttt                                     <  1:1081609/150‑1 (MQ=255)
                                                                                                                        ggTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCAt                              <  1:429720/150‑1 (MQ=255)
                                                                                                                         gTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATg                             <  1:3739669/150‑1 (MQ=255)
                                                                                                                          tACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATgg                            >  1:1597076/1‑150 (MQ=255)
                                                                                                                           aCTGTCGGCGATATCGTCCATTGTCTAGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGt                           <  2:1444630/150‑1 (MQ=255)
                                                                                                                            cTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTc                          <  2:361603/150‑1 (MQ=255)
                                                                                                                                 ggCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACAGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCAc                     >  2:3993745/1‑150 (MQ=255)
                                                                                                                                  gCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCAcc                    <  2:2709951/150‑1 (MQ=255)
                                                                                                                                  gCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCAGCCATTTGGTTCATGGTCGCCAcc                    >  2:1420556/1‑150 (MQ=255)
                                                                                                                                   cGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCg                   >  2:98593/1‑150 (MQ=255)
                                                                                                                                   cGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACCCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCg                   >  2:98736/1‑150 (MQ=255)
                                                                                                                                    gATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGt                  >  1:4157950/1‑150 (MQ=255)
                                                                                                                                    gATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGt                  >  2:1126292/1‑150 (MQ=255)
                                                                                                                                      tatCGTCCATTGTCTAGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGttt                >  2:687578/1‑150 (MQ=255)
                                                                                                                                       atCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTg               <  2:644723/150‑1 (MQ=255)
                                                                                                                                           tCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGa           >  2:3273497/1‑150 (MQ=255)
                                                                                                                                             cATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAAc         >  1:2150916/1‑150 (MQ=255)
                                                                                                                                                tGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGtt      <  1:861155/150‑1 (MQ=255)
                                                                                                                                                tGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGtt      >  2:354701/1‑150 (MQ=255)
                                                                                                                                                tGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGtt      >  2:500740/1‑150 (MQ=255)
                                                                                                                                                tGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGAAGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGtt      <  1:534745/150‑1 (MQ=255)
                                                                                                                                                  tCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTAt    >  1:4054851/1‑150 (MQ=255)
                                                                                                                                                    ttGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCa  <  1:2070269/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAATGCGCTGGGTACTGTCGGCGATATCGTCCATTGTCTTGATCACCGTGGTCATCGCCTCCCCACCCTGCACTGCCGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCA  >  CP009273/3211272‑3211569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑