Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,237,920 T→A 10.1% K61* (AAA→TAA)  uxaC ← uronate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,237,9200TA10.1% 797.5 / 62.7 247K61* (AAA→TAA) uxaCuronate isomerase
Reads supporting (aligned to +/- strand):  ref base T (89/133);  new base A (13/12);  total (102/145)
Fisher's exact test for biased strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 253 total aligned reads displayed.

GGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTCGGTATCTA  >  CP009273/3237773‑3238069
                                                                                                                                                   |                                                                                                                                                     
ggCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGt                                                                                                                                                     >  1:3183049/1‑150 (MQ=255)
     aGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGa                                                                                                                                                >  2:3677514/1‑150 (MQ=255)
      gTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGAt                                                                                                                                               >  2:1425160/1‑150 (MQ=255)
       ttCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATc                                                                                                                                              >  2:784759/1‑150 (MQ=255)
            ggTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCtt                                                                                                                                         <  2:3639429/150‑1 (MQ=255)
             gTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCttt                                                                                                                                        <  1:2468534/150‑1 (MQ=255)
               gCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCa                                                                                                                                      <  1:3605122/150‑1 (MQ=255)
                cGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAg                                                                                                                                     <  2:4062791/150‑1 (MQ=255)
                cGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTAGTAGTGATCGCCTTTCAg                                                                                                                                     >  2:3373183/1‑150 (MQ=255)
                  tCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGcc                                                                                                                                   >  1:4125501/1‑150 (MQ=255)
                    cAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAg                                                                                                                                 <  1:1042715/150‑1 (MQ=255)
                     aGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGa                                                                                                                                >  2:3272628/1‑150 (MQ=255)
                      gTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGAt                                                                                                                               <  1:3745504/150‑1 (MQ=255)
                      gTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGAt                                                                                                                               <  1:4159954/150‑1 (MQ=255)
                       tGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATg                                                                                                                              <  1:1819501/150‑1 (MQ=255)
                         gTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTAGTAGTGATCGCCTTTCAGCCAGATGTc                                                                                                                            >  1:1453469/1‑150 (MQ=255)
                           ataACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCAt                                                                                                                          >  1:1294110/1‑150 (MQ=255)
                            taACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATa                                                                                                                         >  2:2747777/1‑150 (MQ=255)
                            taACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATa                                                                                                                         <  1:2244101/150‑1 (MQ=255)
                               cGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAg                                                                                                                      <  1:528543/150‑1 (MQ=255)
                                 ggTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGt                                                                                                                    <  2:3444775/150‑1 (MQ=255)
                                  gTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGtt                                                                                                                   <  1:2946115/150‑1 (MQ=255)
                                   ttGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGTTCATACTCATCACCTGTAAACATACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTTTAGTGATCGCCTTTCAGCCAGATGTCATACAGGttt                                                                                                                  >  1:1778020/1‑150 (MQ=255)
                                   ttGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTAGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGttt                                                                                                                  <  1:3627310/150‑1 (MQ=255)
                                      ccGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTa                                                                                                               <  1:1110754/150‑1 (MQ=255)
                                      ccGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTa                                                                                                               <  1:1110769/150‑1 (MQ=255)
                                        gATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTAGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTaaa                                                                                                             >  2:1433617/1‑150 (MQ=255)
                                          tAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATCGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAAc                                                                                                           >  1:2872085/1‑150 (MQ=255)
                                          tAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAAc                                                                                                           >  1:4136716/1‑150 (MQ=255)
                                          tAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAAc                                                                                                           <  1:2649439/150‑1 (MQ=255)
                                           aGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACg                                                                                                          <  1:1929219/150‑1 (MQ=255)
                                             tgtgCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGAt                                                                                                        <  2:100398/150‑1 (MQ=255)
                                             tgtgCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGAt                                                                                                        >  1:2036781/1‑150 (MQ=255)
                                                gCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGt                                                                                                     <  1:2040944/150‑1 (MQ=255)
                                                 cGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATCGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTc                                                                                                    >  2:3729830/1‑150 (MQ=255)
                                                 cGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTc                                                                                                    >  2:2151355/1‑150 (MQ=255)
                                                  ggAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCt                                                                                                   >  1:172193/1‑150 (MQ=255)
                                                    aaCAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTc                                                                                                 <  2:1412661/150‑1 (MQ=255)
                                                    aaCAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTc                                                                                                 >  2:2314893/1‑150 (MQ=255)
                                                     aCAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTcc                                                                                                <  2:3928544/150‑1 (MQ=255)
                                                       aGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGc                                                                                              >  1:1137988/1‑150 (MQ=255)
                                                       aGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGACATACAGGTTTTTAAAAAGAAAGTCTTCCga                                                                                              >  2:463027/1‑149 (MQ=255)
                                                          cgccgcCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAAt                                                                                           <  2:2133388/150‑1 (MQ=255)
                                                            ccgccCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAACCGATAGTCTTCCGCAATct                                                                                         <  2:1806835/150‑1 (MQ=255)
                                                            ccgccCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATct                                                                                         <  2:1143131/150‑1 (MQ=255)
                                                            ccgccCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATct                                                                                         >  1:765400/1‑150 (MQ=255)
                                                              gccCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATctgc                                                                                       >  2:2726972/1‑150 (MQ=255)
                                                               cccAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATctgct                                                                                      <  2:3197898/150‑1 (MQ=255)
                                                               cccAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATctgct                                                                                      <  1:882022/150‑1 (MQ=255)
                                                               cccAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATctgct                                                                                      <  2:3762608/150‑1 (MQ=255)
                                                                ccAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATctgctg                                                                                     >  2:2634838/1‑150 (MQ=255)
                                                                  aGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTAGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTgcg                                                                                   <  1:728970/150‑1 (MQ=255)
                                                                     cGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTgcggcg                                                                                <  1:2807247/150‑1 (MQ=255)
                                                                                tCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCaa                                                                     >  2:1182875/1‑150 (MQ=255)
                                                                                 cACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAAt                                                                    >  1:441988/1‑150 (MQ=255)
                                                                                 cACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAAt                                                                    <  2:2937815/150‑1 (MQ=255)
                                                                                    ggTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGt                                                                 >  1:1760210/1‑150 (MQ=255)
                                                                                    ggTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGt                                                                 <  1:786633/150‑1 (MQ=255)
                                                                                     gTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTa                                                                <  2:3088384/150‑1 (MQ=255)
                                                                                      tCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTaa                                                               >  2:3171756/1‑150 (MQ=255)
                                                                                      tCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTaa                                                               <  1:3996218/150‑1 (MQ=255)
                                                                                       cAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAAt                                                              <  2:524034/150‑1 (MQ=255)
                                                                                        aGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATc                                                             >  1:1365817/1‑150 (MQ=255)
                                                                                        aGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATc                                                             <  1:2437767/150‑1 (MQ=255)
                                                                                        aGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATc                                                             <  2:2509031/150‑1 (MQ=255)
                                                                                        aGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATc                                                             <  2:2508336/150‑1 (MQ=255)
                                                                                         gACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCg                                                            >  1:3915383/1‑150 (MQ=255)
                                                                                          aCGCATCACCGGTACACAGACGCTCGACCACACCGTTGGTACGCATAGCGCGCCATTAGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGa                                                           >  1:3948602/1‑150 (MQ=255)
                                                                                              aTCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAAt                                                       >  2:522935/1‑150 (MQ=255)
                                                                                               tCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATc                                                      <  1:3587817/150‑1 (MQ=255)
                                                                                               tCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATc                                                      <  2:1605890/150‑1 (MQ=255)
                                                                                                   cGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCt                                                  <  1:628566/150‑1 (MQ=255)
                                                                                                     gTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTgg                                                <  1:2351969/150‑1 (MQ=255)
                                                                                                       acacAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTc                                              <  1:1615753/150‑1 (MQ=255)
                                                                                                         acaGACGCTCGGCCACACCGTTGTTACGCATTGCGCGCAATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATAGGCTGGTCtt                                            <  2:2428425/150‑1 (MQ=255)
                                                                                                          caGACGCTCGGCCACACAGTTGGTACGCTTAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTATAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCttt                                           <  2:1733085/150‑1 (MQ=255)
                                                                                                              cGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCg                                       >  2:203385/1‑150 (MQ=255)
                                                                                                              cGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCg                                       >  2:390758/1‑150 (MQ=255)
                                                                                                              cGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCg                                       <  1:564905/150‑1 (MQ=255)
                                                                                                                  cGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGt                                   <  1:2511791/150‑1 (MQ=255)
                                                                                                                   ggCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTc                                  <  1:1424978/150‑1 (MQ=255)
                                                                                                                    gCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCg                                 >  1:306553/1‑150 (MQ=255)
                                                                                                                       acacCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGa                              >  1:255072/1‑150 (MQ=255)
                                                                                                                           cGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACa                          <  2:2388788/150‑1 (MQ=255)
                                                                                                                            gTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAg                         <  1:1646030/150‑1 (MQ=255)
                                                                                                                             ttGGTACGCATAGCGCGCCATTAGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGa                        <  1:3316076/150‑1 (MQ=255)
                                                                                                                                gTACGCATAGCGCGCCATTAGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGAcgg                     <  2:1279432/150‑1 (MQ=255)
                                                                                                                                    gCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCggg                 >  1:1975065/1‑150 (MQ=255)
                                                                                                                                      aTAGCGCGCCATTAGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCa               >  1:2235230/1‑150 (MQ=255)
                                                                                                                                         gcgcgcCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAAt            <  2:1520892/150‑1 (MQ=255)
                                                                                                                                           gcgcCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTc          <  1:23577/150‑1 (MQ=255)
                                                                                                                                           gcgcCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTc          <  2:2197215/150‑1 (MQ=255)
                                                                                                                                           gcgcCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTCCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTc          <  2:3412030/150‑1 (MQ=255)
                                                                                                                                              ccATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTCGGt       >  2:1055520/1‑150 (MQ=255)
                                                                                                                                              ccATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTCGGt       <  1:1186285/150‑1 (MQ=255)
                                                                                                                                               cATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTCGGTa      <  2:767495/150‑1 (MQ=255)
                                                                                                                                                aTTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTCGGTAt     >  1:1008225/1‑150 (MQ=255)
                                                                                                                                                 tttGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTCGGTATc    <  2:939450/150‑1 (MQ=255)
                                                                                                                                                 tttGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTCGGTATc    >  1:3467159/1‑150 (MQ=255)
                                                                                                                                                   tGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTCGGTATCTa  <  1:1727973/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGCGCAGTTCGAGGTGCGTCCAGTGGTATAACGGGTTGCCGATAGTGTGCGGAACAGTCGCCGCCCAGGCGTCAAATTTTTCACGGTCAGACGCATCACCGGTACACAGACGCTCGGCCACACCGTTGGTACGCATAGCGCGCCATTTGTAGTGATCGCCTTTCAGCCAGATGTCATACAGGTTTTTAAAACGATAGTCTTCCGCAATCTGCTGCGGCGGCAAATGGCAATGGTAATCGAAAATCGGCTGGTCTTTTGCGTAGTCGTGATACAGACGGCGGGCAAATTCGGTATCTA  >  CP009273/3237773‑3238069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑