Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,252,258 C→A 100% D251Y (GAT→TAT)  tdcG ← L‑serine dehydratase 3, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,252,2580CA99.6% 848.0 / ‑3.9 237D251Y (GAT→TAT) tdcGL‑serine dehydratase 3, anaerobic
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (132/104);  total (133/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 239 total aligned reads displayed.

AATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTT  >  CP009273/3252111‑3252404
                                                                                                                                                   |                                                                                                                                                  
aataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATATGATAGTTAGAGATGTTATAGc                                                                                                                                                  >  1:3676648/1‑150 (MQ=255)
aataaGACAGTACTGCCGGAATAATGCCGCACGCACCGTTAGACGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTATCTTCACAAACCGCCAGCGCGAACATGTTGATCCAGTCGATGACATACATCGGATCGTTAGAGAGGTTATAGc                                                                                                                                                  >  2:77444/1‑150 (MQ=255)
  taaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTg                                                                                                                                                >  1:2501410/1‑150 (MQ=255)
   aaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTgg                                                                                                                                               <  2:1980311/150‑1 (MQ=255)
       cAGTACTGCCGGAATAATGCCGCACGCTCCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAc                                                                                                                                           <  1:3815937/150‑1 (MQ=255)
       cAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAc                                                                                                                                           >  2:2817071/1‑150 (MQ=255)
       cAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAc                                                                                                                                           <  1:1902469/150‑1 (MQ=255)
        aGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAcc                                                                                                                                          <  2:2129896/150‑1 (MQ=255)
          tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAg                                                                                                                                        <  1:636140/150‑1 (MQ=255)
          tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAg                                                                                                                                        <  1:403838/150‑1 (MQ=255)
           aCTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGc                                                                                                                                       >  1:1003071/1‑150 (MQ=255)
             tGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTg                                                                                                                                     <  1:2459061/150‑1 (MQ=255)
              gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTga                                                                                                                                    >  1:2656209/1‑150 (MQ=255)
                cGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTgacg                                                                                                                                  <  1:2042083/150‑1 (MQ=255)
                  gAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTgacgac                                                                                                                                <  1:3110484/150‑1 (MQ=255)
                   aataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGacgacg                                                                                                                               >  2:2976467/1‑150 (MQ=255)
                   aataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGacgacg                                                                                                                               >  1:235567/1‑150 (MQ=255)
                        tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAgcg                                                                                                                          >  1:3349289/1‑150 (MQ=255)
                        tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAgcg                                                                                                                          >  1:3110572/1‑150 (MQ=255)
                          ccGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCt                                                                                                                        <  2:3149181/150‑1 (MQ=255)
                          ccGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCt                                                                                                                        >  2:3025872/1‑150 (MQ=255)
                             cacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCAAGCGCGTACAGGTAGATCCAGACGATGACATTCAACGGATCGTTAGAGAAGTTATAGCTGGAAACCAGCAGACGACGCAGCGCTacg                                                                                                                     >  1:1874767/1‑150 (MQ=255)
                                gcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacggca                                                                                                                  >  1:3889949/1‑150 (MQ=255)
                                gcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacggca                                                                                                                  >  1:2501685/1‑150 (MQ=255)
                                 cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacggcac                                                                                                                 >  1:1081134/1‑150 (MQ=255)
                                 cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacggcac                                                                                                                 >  1:3392442/1‑150 (MQ=255)
                                     gTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGgcg                                                                                                             >  1:4083736/1‑150 (MQ=255)
                                      ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGgcgc                                                                                                            >  1:2989069/1‑150 (MQ=255)
                                       tAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGcgcg                                                                                                           <  2:1014266/150‑1 (MQ=255)
                                        aGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCgg                                                                                                          >  2:392161/1‑150 (MQ=255)
                                             gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACAt                                                                                                     <  2:2064082/150‑1 (MQ=255)
                                                 cgatACCACGCGCCCGCCAGCTGCGTTTTATTCACTAACCGCCAGCGCGTACATGTTGAACCAGTCGATGACATTCATCGGATCGTTAGATAAGTTATAGCTGGAAACCAGCTGACGACGCATCCCTACGGCACGGCGCGGCACATTGAg                                                                                                 >  1:3110702/4‑150 (MQ=37)
                                                   ttACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCg                                                                                               <  1:2876106/150‑1 (MQ=255)
                                                      ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGAc                                                                                            <  1:2746357/150‑1 (MQ=255)
                                                        aCGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCa                                                                                          <  1:1617769/150‑1 (MQ=255)
                                                         cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAg                                                                                         <  2:3409619/150‑1 (MQ=255)
                                                          gcgcCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAgg                                                                                        <  2:2577734/150‑1 (MQ=255)
                                                            gcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgca                                                                                      <  2:2011906/150‑1 (MQ=255)
                                                            gcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgca                                                                                      <  2:368962/150‑1 (MQ=255)
                                                            gcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgca                                                                                      <  1:619519/150‑1 (MQ=255)
                                                            gcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgca                                                                                      <  2:3210056/150‑1 (MQ=255)
                                                                   aGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCCGGCAGCACGcc                                                                               >  1:700367/1‑150 (MQ=255)
                                                                    gCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCt                                                                              >  2:1346775/1‑150 (MQ=255)
                                                                         gTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAgt                                                                         >  2:2680846/1‑150 (MQ=255)
                                                                            ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGtt                                                                      <  1:1525268/150‑1 (MQ=255)
                                                                             tcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTc                                                                     >  1:3864015/1‑150 (MQ=255)
                                                                             tcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTc                                                                     >  1:40454/1‑150 (MQ=255)
                                                                               ttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCAt                                                                   <  1:2527866/150‑1 (MQ=255)
                                                                                tcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATc                                                                  >  2:1806299/1‑150 (MQ=255)
                                                                                 cACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATcc                                                                 >  2:2333688/1‑150 (MQ=255)
                                                                                 cACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATcc                                                                 <  2:897569/150‑1 (MQ=255)
                                                                                  aCTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATccc                                                                >  2:385323/1‑150 (MQ=255)
                                                                                  aCTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATccc                                                                >  2:1614796/1‑150 (MQ=255)
                                                                                   cTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCa                                                               <  1:1434062/150‑1 (MQ=255)
                                                                                        cGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTc                                                          >  1:1548941/1‑150 (MQ=255)
                                                                                        cGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTc                                                          >  2:3535729/1‑150 (MQ=255)
                                                                                           cAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAAt                                                       <  2:2813321/150‑1 (MQ=255)
                                                                                           cAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAAt                                                       >  2:4040693/1‑150 (MQ=255)
                                                                                              cgcgTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCCGTGTTCATCCCACGTTCAATAcc                                                    <  2:2902781/150‑1 (MQ=255)
                                                                                              cgcgTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATAcc                                                    <  2:2053569/150‑1 (MQ=255)
                                                                                              cgcgTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATAcc                                                    <  2:1259167/150‑1 (MQ=255)
                                                                                              cgcgTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATAcc                                                    <  2:1042663/150‑1 (MQ=255)
                                                                                              cgcgTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGAAGACGCAGAGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGATCATCCCACGTTCAATAcc                                                    >  2:1553517/1‑150 (MQ=255)
                                                                                                 gTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTc                                                 >  2:1412915/1‑150 (MQ=255)
                                                                                                 gTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTc                                                 >  2:3703230/1‑150 (MQ=255)
                                                                                                  tACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCa                                                <  1:779629/150‑1 (MQ=255)
                                                                                                    cATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATg                                              >  1:1797169/1‑150 (MQ=255)
                                                                                                    cATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATg                                              >  1:938451/1‑150 (MQ=255)
                                                                                                     aTGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGc                                             >  1:328623/1‑150 (MQ=255)
                                                                                                        ttGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATc                                          <  2:1712313/150‑1 (MQ=255)
                                                                                                        ttGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATc                                          <  2:594204/150‑1 (MQ=255)
                                                                                                          gATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCAc                                        >  1:2270515/1‑150 (MQ=255)
                                                                                                           aTCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACt                                       >  2:3228543/1‑150 (MQ=255)
                                                                                                             ccAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTg                                     >  1:4169461/1‑150 (MQ=255)
                                                                                                               aGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGcc                                   >  1:1213122/1‑150 (MQ=255)
                                                                                                                   gATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGAt                               >  2:376252/1‑150 (MQ=255)
                                                                                                                      gACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACg                            <  1:455744/150‑1 (MQ=255)
                                                                                                                      gACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACg                            >  1:2135711/1‑150 (MQ=255)
                                                                                                                      gACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACg                            >  1:2935202/1‑150 (MQ=255)
                                                                                                                        cATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACggg                          >  2:3666129/1‑150 (MQ=255)
                                                                                                                         aTTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGc                         >  1:830816/1‑150 (MQ=255)
                                                                                                                                ggATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCg                  <  1:2510960/150‑1 (MQ=255)
                                                                                                                                 gATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCgg                 <  2:230851/150‑1 (MQ=255)
                                                                                                                                 gATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCgg                 >  2:1116655/1‑150 (MQ=255)
                                                                                                                                  aTCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGc                >  2:874759/1‑150 (MQ=255)
                                                                                                                                  aTCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGc                >  2:362446/1‑150 (MQ=255)
                                                                                                                                  aTCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGc                >  1:2615430/1‑150 (MQ=255)
                                                                                                                                    cGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGt              <  1:1683952/150‑1 (MQ=255)
                                                                                                                                    cGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGt              >  1:914689/1‑150 (MQ=255)
                                                                                                                                     gTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTc             <  1:3784948/150‑1 (MQ=255)
                                                                                                                                     gTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTc             >  1:1168115/1‑150 (MQ=255)
                                                                                                                                       tAGAGATGTTATAGCTGGAAACCATCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCaa           >  1:1041070/1‑150 (MQ=255)
                                                                                                                                       tAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCaa           >  2:3336528/1‑150 (MQ=255)
                                                                                                                                       tAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCaa           >  1:3476177/1‑150 (MQ=255)
                                                                                                                                        agagATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAt          >  1:2468327/1‑150 (MQ=255)
                                                                                                                                        agagATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAt          <  2:2424324/150‑1 (MQ=255)
                                                                                                                                         gagaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAtt         >  2:1867654/1‑150 (MQ=255)
                                                                                                                                          agaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAtct        <  1:2641265/150‑3 (MQ=255)
                                                                                                                                           gaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTc       <  1:2805243/150‑1 (MQ=255)
                                                                                                                                                tataGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCtt  >  1:3837979/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTT  >  CP009273/3252111‑3252404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑