Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,334,892 G→T 25.2% D90Y (GAT→TAT)  kdsD → D‑arabinose 5‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,334,8920GT25.2% 510.4 / 137.3 210D90Y (GAT→TAT) kdsDD‑arabinose 5‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (80/77);  new base T (25/28);  total (105/105)
Fisher's exact test for biased strand distribution p-value = 7.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

TTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGGCGCGC  >  CP009273/3334743‑3335041
                                                                                                                                                     |                                                                                                                                                     
ttGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTg                                                                                                                                                       <  1:3605341/150‑1 (MQ=255)
ttGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTg                                                                                                                                                       >  2:1070948/1‑150 (MQ=255)
 tGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACAGCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGa                                                                                                                                                      >  2:2246961/1‑150 (MQ=255)
    cTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGAttt                                                                                                                                                   <  2:1154543/150‑1 (MQ=255)
      gtgAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAg                                                                                                                                                 <  2:1641392/150‑1 (MQ=255)
        gAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGc                                                                                                                                               >  1:38715/1‑150 (MQ=255)
           aaGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATg                                                                                                                                            >  1:3246592/1‑150 (MQ=255)
           aaGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATg                                                                                                                                            >  2:2514079/1‑150 (MQ=255)
             gATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGt                                                                                                                                          >  2:378196/1‑150 (MQ=255)
               tGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTa                                                                                                                                        <  1:2944837/150‑1 (MQ=255)
                gTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTAc                                                                                                                                       >  1:1449200/1‑150 (MQ=255)
                    tGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCca                                                                                                                                   <  2:2275095/150‑1 (MQ=255)
                       tgtAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAg                                                                                                                                <  2:2717734/150‑1 (MQ=255)
                        gtAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTATTCGTCCATCCTGGTGAATCCGAGCATGGTGATTTAGGCATGGTTACCCCACAgg                                                                                                                               >  1:2565514/1‑150 (MQ=255)
                         tAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGa                                                                                                                              <  2:2018020/150‑1 (MQ=255)
                           aaGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGAtg                                                                                                                            <  2:1976961/150‑1 (MQ=255)
                           aaGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGAtg                                                                                                                            >  1:578511/1‑150 (MQ=255)
                           aaGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGAtg                                                                                                                            >  1:1255505/1‑150 (MQ=255)
                            aGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGAtgt                                                                                                                           <  2:420104/150‑1 (MQ=255)
                             gggAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGAtgtg                                                                                                                          <  2:3629983/150‑1 (MQ=255)
                                  aGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGAt                                                                                                                     <  2:1482741/150‑1 (MQ=255)
                                  aGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGAt                                                                                                                     <  1:981234/150‑1 (MQ=255)
                                  aGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGAt                                                                                                                     >  2:3860255/1‑150 (MQ=255)
                                   gTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGAtt                                                                                                                    >  1:81983/1‑150 (MQ=255)
                                        cgtcATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTAt                                                                                                               <  2:4118557/150‑1 (MQ=255)
                                        cgtcATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTAt                                                                                                               <  1:3646645/150‑1 (MQ=255)
                                             tGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTa                                                                                                          <  2:1280201/150‑1 (MQ=255)
                                                ggATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAAct                                                                                                       <  1:2188122/150‑1 (MQ=255)
                                                  aTGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAActct                                                                                                     >  1:2658437/1‑150 (MQ=255)
                                                  aTGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAActct                                                                                                     >  1:3058989/1‑150 (MQ=255)
                                                   tGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCACCGTTTGCCAGCACCGGTACACCGTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTg                                                                                                    >  2:3521270/1‑150 (MQ=255)
                                                       aaaaTCGGGGCATAGTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGa                                                                                                <  1:3407492/150‑1 (MQ=255)
                                                          aTCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATc                                                                                             <  1:1186601/150‑1 (MQ=255)
                                                          aTCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATc                                                                                             <  2:2593799/150‑1 (MQ=255)
                                                           tCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATcc                                                                                            <  1:3908690/150‑1 (MQ=255)
                                                            cGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCa                                                                                           <  2:459040/150‑1 (MQ=255)
                                                            cGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCa                                                                                           >  2:3524443/1‑150 (MQ=255)
                                                             ggggCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAg                                                                                          <  1:621444/150‑1 (MQ=255)
                                                               ggCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCg                                                                                        <  1:1859891/150‑1 (MQ=255)
                                                               ggCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCg                                                                                        <  1:3032746/150‑1 (MQ=255)
                                                                gCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGa                                                                                       >  1:234047/1‑150 (MQ=255)
                                                                 cATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGaa                                                                                      >  2:2614262/1‑150 (MQ=255)
                                                                   tatTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAAt                                                                                    <  2:3713823/150‑1 (MQ=255)
                                                                      tGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCAc                                                                                 >  2:1581384/1‑150 (MQ=255)
                                                                       gggCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACg                                                                                <  1:2233079/150‑1 (MQ=255)
                                                                       gggCGAAAAATGGCGGCAACGTGTGCCAGCACCGGTACACCTTCCTTTTTCGTCCATCCTGGTGTAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACg                                                                                <  2:3832939/150‑1 (MQ=255)
                                                                           gAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTATGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCt                                                                            <  1:3194432/150‑1 (MQ=255)
                                                                           gAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCt                                                                            <  1:916115/150‑1 (MQ=255)
                                                                            aaaaaTGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCtt                                                                           <  1:1716161/150‑1 (MQ=255)
                                                                            aaaaaTGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCtg                                                                           >  1:2311150/1‑149 (MQ=255)
                                                                             aaaaTGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTa                                                                          >  1:2056098/1‑150 (MQ=255)
                                                                              aaaTGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTaa                                                                         >  2:1379006/1‑150 (MQ=255)
                                                                               aaTGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAAt                                                                        >  2:744236/1‑150 (MQ=255)
                                                                               aaTGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAAt                                                                        >  1:1659412/1‑150 (MQ=255)
                                                                                aTGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAAtt                                                                       <  2:3109326/150‑1 (MQ=255)
                                                                                 tGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTc                                                                      >  1:2369867/1‑150 (MQ=255)
                                                                                 tGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTc                                                                      <  2:2349271/150‑1 (MQ=255)
                                                                                 tGGCGGCAACGTTTACCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTc                                                                      >  1:3719282/1‑150 (MQ=255)
                                                                                  ggcggcAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTcc                                                                     >  2:3972330/1‑150 (MQ=255)
                                                                                   gcggcAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCa                                                                    <  2:698243/150‑1 (MQ=255)
                                                                                    cggcAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAg                                                                   <  1:3521961/150‑1 (MQ=255)
                                                                                      gcAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTg                                                                 >  2:2252288/1‑150 (MQ=255)
                                                                                      gcAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTg                                                                 <  1:67388/150‑1 (MQ=255)
                                                                                       cAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCCGTGc                                                                >  2:3609908/1‑150 (MQ=255)
                                                                                        aaCGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCt                                                               <  2:1123279/150‑1 (MQ=255)
                                                                                        aaCGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCt                                                               <  2:4016309/150‑1 (MQ=255)
                                                                                          cGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTa                                                             >  1:3460330/1‑150 (MQ=255)
                                                                                           gTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCGTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTaa                                                            >  1:537408/1‑150 (MQ=255)
                                                                                           gTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTaa                                                            <  1:1281044/150‑1 (MQ=255)
                                                                                            tttGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAg                                                           <  2:1016862/150‑1 (MQ=255)
                                                                                                 cAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCt                                                      >  1:2999050/1‑150 (MQ=255)
                                                                                                 cAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCt                                                      <  1:1418455/150‑1 (MQ=255)
                                                                                                  aGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCtt                                                     >  2:2452255/1‑150 (MQ=255)
                                                                                                   gCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTc                                                    <  1:2889482/150‑1 (MQ=255)
                                                                                                       cGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGt                                                >  2:1639979/1‑150 (MQ=255)
                                                                                                        ggTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTa                                               >  2:2899043/1‑150 (MQ=255)
                                                                                                        ggTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTa                                               >  2:3095673/1‑150 (MQ=255)
                                                                                                              ccTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTa                                         <  2:1850258/150‑1 (MQ=255)
                                                                                                              ccTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTa                                         <  2:2396905/150‑1 (MQ=255)
                                                                                                               cTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTaa                                        <  1:1969669/150‑1 (MQ=255)
                                                                                                               cTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTaa                                        >  1:334608/1‑150 (MQ=255)
                                                                                                                ttCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAAt                                       <  2:3938718/150‑1 (MQ=255)
                                                                                                                 tCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATc                                      >  2:3016283/1‑150 (MQ=255)
                                                                                                                   aTTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTg                                    >  2:2336393/1‑150 (MQ=255)
                                                                                                                      tttCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCAt                                 <  2:3060752/150‑1 (MQ=255)
                                                                                                                      tttCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCAt                                 <  2:2796023/150‑1 (MQ=255)
                                                                                                                         cGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCAc                              >  2:3354315/1‑150 (MQ=255)
                                                                                                                            ccATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCgg                           >  2:1813053/1‑150 (MQ=255)
                                                                                                                            ccATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCgg                           >  1:70185/1‑150 (MQ=255)
                                                                                                                                  tGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGccc                     <  2:2182655/150‑1 (MQ=255)
                                                                                                                                       aaGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGgaga                >  1:3746599/1‑150 (MQ=255)
                                                                                                                                       aaGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGgaga                >  2:1953557/1‑150 (MQ=255)
                                                                                                                                        aGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGagag               >  2:3915015/1‑150 (MQ=255)
                                                                                                                                         gCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGagc              <  1:1070948/150‑1 (MQ=255)
                                                                                                                                           cgcgCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGagcag            >  1:3625910/1‑150 (MQ=255)
                                                                                                                                             cgcATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAgcagca          >  2:2030354/1‑150 (MQ=255)
                                                                                                                                               cATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATg        >  1:2498083/1‑150 (MQ=255)
                                                                                                                                               cATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATg        <  2:4926/150‑1 (MQ=255)
                                                                                                                                                aTGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATgg       >  1:4108879/1‑150 (MQ=255)
                                                                                                                                                     gATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGgcgcgc  >  1:1329527/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGGCGCGC  >  CP009273/3334743‑3335041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑