Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,365,607 G→T 100% I109I (ATC→ATA)  nanT ← sialic acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,365,6070GT98.9% 944.3 / 1.1 273I109I (ATC→ATA) nanTsialic acid transporter
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (144/126);  total (147/126)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 273 total aligned reads displayed.

CGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAGAGAT  >  CP009273/3365459‑3365753
                                                                                                                                                    |                                                                                                                                                  
cgacgGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGa                                                                                                                                                   >  1:3511334/1‑150 (MQ=255)
 gacgGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTAt                                                                                                                                                  <  1:4162244/150‑1 (MQ=255)
  acgGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATg                                                                                                                                                 <  1:2991822/150‑1 (MQ=255)
        cccACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCt                                                                                                                                           <  2:679899/150‑1 (MQ=255)
        cccACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCt                                                                                                                                           <  2:3231374/150‑1 (MQ=255)
           acaGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGgc                                                                                                                                        <  2:3451670/150‑1 (MQ=255)
             agagAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGgcgc                                                                                                                                      >  2:119470/1‑150 (MQ=255)
               agaAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCaa                                                                                                                                    >  2:4014726/1‑150 (MQ=255)
                 aaGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAg                                                                                                                                  >  2:123743/1‑150 (MQ=255)
                 aaGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAg                                                                                                                                  >  1:2616927/1‑150 (MQ=255)
                      tGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCa                                                                                                                             <  1:3138065/150‑1 (MQ=255)
                       gAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAg                                                                                                                            <  1:2617336/150‑1 (MQ=255)
                           tCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCa                                                                                                                        <  1:1127614/150‑1 (MQ=255)
                            cAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAg                                                                                                                       <  2:2985010/150‑1 (MQ=255)
                            cAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAg                                                                                                                       >  2:2428574/1‑150 (MQ=255)
                              aaaaaCCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCg                                                                                                                     >  1:687598/1‑150 (MQ=255)
                                  aCCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTccc                                                                                                                 >  2:177445/1‑150 (MQ=255)
                                  aCCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTccc                                                                                                                 >  2:3077805/1‑150 (MQ=255)
                                  aCCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTccc                                                                                                                 >  2:2800020/1‑150 (MQ=255)
                                      cTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGcc                                                                                                             >  2:3358045/1‑150 (MQ=255)
                                        ggCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCga                                                                                                           >  2:1681279/1‑150 (MQ=255)
                                          cTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgaga                                                                                                         >  1:1477753/1‑150 (MQ=255)
                                           tttGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaa                                                                                                        <  2:592002/150‑1 (MQ=255)
                                            ttGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaag                                                                                                       <  1:2064605/150‑1 (MQ=255)
                                              gTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagag                                                                                                     <  1:2257660/150‑1 (MQ=255)
                                               ttACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCCGCGTCCCGGCCgagaagaga                                                                                                    >  1:2635940/1‑150 (MQ=255)
                                               ttACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagaga                                                                                                    <  2:2345096/150‑1 (MQ=255)
                                               ttACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagaga                                                                                                    <  2:2182094/150‑1 (MQ=255)
                                               ttACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagaga                                                                                                    >  1:1043942/1‑150 (MQ=255)
                                               ttACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagaga                                                                                                    >  2:882181/1‑150 (MQ=255)
                                               ttACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagaga                                                                                                    >  1:2960218/1‑150 (MQ=255)
                                                tACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagagaa                                                                                                   >  1:430031/1‑150 (MQ=255)
                                                     aGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATg                                                                                              <  2:2629570/150‑1 (MQ=255)
                                                     aGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATg                                                                                              >  2:2774685/1‑150 (MQ=255)
                                                     aGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATg                                                                                              >  1:3123259/1‑150 (MQ=255)
                                                       aTGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCt                                                                                            <  1:3557493/150‑1 (MQ=255)
                                                                   gCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGACGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGc                                                                                <  1:817143/150‑1 (MQ=255)
                                                                    cTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGcc                                                                               >  2:2486200/1‑150 (MQ=255)
                                                                    cTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGcc                                                                               >  2:2486238/1‑150 (MQ=255)
                                                                      ttCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAg                                                                             <  1:1656181/150‑1 (MQ=255)
                                                                        cAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAgac                                                                           <  1:493222/150‑1 (MQ=255)
                                                                         aaTGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAgacg                                                                          >  2:1283389/1‑150 (MQ=255)
                                                                           tGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAgacgac                                                                        <  1:907097/150‑1 (MQ=255)
                                                                            gACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGacgacg                                                                       >  1:3473597/1‑150 (MQ=255)
                                                                               aTAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGccc                                                                    <  1:4097360/150‑1 (MQ=255)
                                                                                   gTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAg                                                                >  1:2642927/1‑150 (MQ=255)
                                                                                     ggCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCg                                                              >  2:363084/1‑150 (MQ=255)
                                                                                       cgcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGt                                                            <  1:3446883/150‑1 (MQ=255)
                                                                                          tGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCAc                                                         >  1:2121301/1‑150 (MQ=255)
                                                                                          tGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCAc                                                         <  1:93030/150‑1 (MQ=255)
                                                                                          tGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCAc                                                         >  2:2908460/1‑150 (MQ=255)
                                                                                           ggAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCAcc                                                        >  1:885093/1‑150 (MQ=255)
                                                                                             aaCCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCa                                                      >  2:2519127/1‑150 (MQ=255)
                                                                                              aCCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCAt                                                     >  1:3878871/1‑150 (MQ=255)
                                                                                               ccGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATa                                                    <  2:2001272/150‑1 (MQ=255)
                                                                                                cGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAg                                                   <  2:3505676/150‑1 (MQ=255)
                                                                                                cGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAg                                                   >  1:3369926/1‑150 (MQ=255)
                                                                                                 gTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAgc                                                  >  2:2721414/1‑150 (MQ=255)
                                                                                                 gTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAgc                                                  >  2:631534/1‑150 (MQ=255)
                                                                                                 gTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAgc                                                  >  2:2203150/1‑150 (MQ=255)
                                                                                                 gTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAgc                                                  >  1:3163447/1‑150 (MQ=255)
                                                                                                  tATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAgcg                                                 <  1:900343/150‑1 (MQ=255)
                                                                                                    ttCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGcc                                               <  2:4172921/150‑1 (MQ=255)
                                                                                                     tCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCg                                              <  1:247488/150‑1 (MQ=255)
                                                                                                     tCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCg                                              >  2:530085/1‑150 (MQ=255)
                                                                                                       aCCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAg                                            >  2:2667356/1‑150 (MQ=255)
                                                                                                           gCCACCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATc                                        >  2:1516626/1‑150 (MQ=255)
                                                                                                            ccATCCCCCTGCCGATGCCCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGCCGCCCGTAGCGGTCACCCATAGCGCCGAGCATCa                                       <  2:3900215/150‑1 (MQ=255)
                                                                                                            ccATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCa                                       >  2:3811738/1‑150 (MQ=255)
                                                                                                               tCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgc                                    >  1:2395913/1‑150 (MQ=255)
                                                                                                               tCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgc                                    >  1:1015953/1‑150 (MQ=255)
                                                                                                                ccccATGCCGATGACCAGACGAGCGATAAACCTGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgcc                                   <  2:525926/150‑1 (MQ=255)
                                                                                                                  ccATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgccgc                                 >  1:2808894/1‑150 (MQ=255)
                                                                                                                   cATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgccgcc                                <  1:1871612/150‑1 (MQ=255)
                                                                                                                   cATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgccgcc                                >  2:1491451/1‑150 (MQ=255)
                                                                                                                    aTGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGccgccg                               <  1:3313633/150‑1 (MQ=255)
                                                                                                                        cGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAAcc                           <  2:1812796/150‑1 (MQ=255)
                                                                                                                           tGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGc                        >  1:3538937/1‑150 (MQ=255)
                                                                                                                           tGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGc                        <  1:4128539/150‑1 (MQ=255)
                                                                                                                            gACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCg                       <  1:874613/150‑1 (MQ=255)
                                                                                                                             aCCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCga                      <  1:2547944/150‑1 (MQ=255)
                                                                                                                               cAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgaga                    <  1:1117031/150‑1 (MQ=255)
                                                                                                                               cAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgaga                    >  2:3959646/1‑150 (MQ=255)
                                                                                                                                aGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgagag                   <  2:4022068/150‑1 (MQ=255)
                                                                                                                                  aCGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGAt                 >  1:595016/1‑150 (MQ=255)
                                                                                                                                   cGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATa                >  2:276609/1‑150 (MQ=255)
                                                                                                                                     aGCGATATACATGGTTATGGAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACTATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCTCCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGATATaaa              <  2:190461/150‑1 (MQ=255)
                                                                                                                                     aGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATaaa              >  1:2994880/1‑150 (MQ=255)
                                                                                                                                       cGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAgg            >  1:3974368/1‑150 (MQ=255)
                                                                                                                                       cGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAgg            <  1:3956704/150‑1 (MQ=255)
                                                                                                                                       cGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAgg            <  1:2734554/150‑1 (MQ=255)
                                                                                                                                         aTAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCt          <  1:1936824/150‑1 (MQ=255)
                                                                                                                                          tAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTg         <  2:2125992/150‑1 (MQ=255)
                                                                                                                                          tAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTg         <  2:3142779/150‑1 (MQ=255)
                                                                                                                                          tAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTg         <  1:3681991/150‑1 (MQ=255)
                                                                                                                                           aaaCATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGc        >  1:37628/1‑150 (MQ=255)
                                                                                                                                             aCATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCag      >  1:544751/1‑150 (MQ=255)
                                                                                                                                              cATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCaga     >  2:1883638/1‑150 (MQ=255)
                                                                                                                                                 ggTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAGAGAt  <  1:2945494/150‑1 (MQ=255)
                                                                                                                                                 ggTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAGAGAt  <  1:2941135/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAGAGAT  >  CP009273/3365459‑3365753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑