Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,425,514 C→A 6.3% E104* (GAA→TAA)  smg ← DUF494 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,425,5140CA 6.3% 981.9 / 28.2 252E104* (GAA→TAA) smgDUF494 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (120/116);  new base A (10/6);  total (130/122)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 255 total aligned reads displayed.

TTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCT  >  CP009273/3425365‑3425663
                                                                                                                                                     |                                                                                                                                                     
ttcattcaCTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTc                                                                                                                                                       >  2:1110784/1‑150 (MQ=255)
  cattcaCTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTAAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCAc                                                                                                                                                     >  1:3601191/1‑150 (MQ=255)
   attcaCTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACg                                                                                                                                                    >  1:3765496/1‑150 (MQ=255)
   attcaCTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACg                                                                                                                                                    <  2:2154391/150‑1 (MQ=255)
     tcaCTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAg                                                                                                                                                  <  2:1796575/150‑1 (MQ=255)
        cTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGttt                                                                                                                                               >  1:657607/1‑150 (MQ=255)
        cTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGttt                                                                                                                                               <  2:904253/150‑1 (MQ=255)
         ttCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTc                                                                                                                                              <  2:2580422/150‑1 (MQ=255)
            aaaGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAg                                                                                                                                           >  1:161782/1‑150 (MQ=255)
             aaGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAgg                                                                                                                                          >  2:2629603/1‑150 (MQ=255)
               gagTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGtt                                                                                                                                        <  2:2279533/150‑1 (MQ=255)
                agTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTg                                                                                                                                       <  2:2850128/150‑1 (MQ=255)
                   aaTTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGc                                                                                                                                    >  1:2847354/1‑150 (MQ=255)
                   aaTTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGc                                                                                                                                    <  1:3231990/150‑1 (MQ=255)
                    aTTCTTCCTTTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCTCCCATTTCAGATCTTCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCa                                                                                                                                   <  1:487195/150‑1 (MQ=255)
                    aTTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCa                                                                                                                                   >  2:624006/1‑150 (MQ=255)
                      tcttcCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCGGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCAcc                                                                                                                                 >  2:2078321/1‑150 (MQ=255)
                       cttcCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCt                                                                                                                                <  1:83290/150‑1 (MQ=255)
                                gCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTAACGAGTTTCAAGGTTGAGCACCTGAATCTGCt                                                                                                                       >  2:80278/1‑150 (MQ=255)
                                   ggTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCaa                                                                                                                    >  2:2394266/1‑150 (MQ=255)
                                   ggTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCaa                                                                                                                    >  1:2282188/1‑150 (MQ=255)
                                    gTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAg                                                                                                                   <  2:444875/150‑1 (MQ=255)
                                            tttCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCa                                                                                                           >  1:3322138/1‑150 (MQ=255)
                                             ttCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATATCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAg                                                                                                          >  2:3601846/1‑150 (MQ=255)
                                              tCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGa                                                                                                         >  1:1225344/1‑150 (MQ=255)
                                               cGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGaa                                                                                                        >  2:3492535/1‑150 (MQ=255)
                                                 cAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAAc                                                                                                      >  1:3372545/1‑150 (MQ=255)
                                                 cAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAAc                                                                                                      <  1:4067538/150‑1 (MQ=255)
                                                 cAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAAc                                                                                                      <  2:3092877/150‑1 (MQ=255)
                                                  aGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAccc                                                                                                     >  2:150530/1‑150 (MQ=255)
                                                  aGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAAcc                                                                                                     >  1:473622/1‑150 (MQ=255)
                                                  aGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAAcc                                                                                                     >  1:2556334/1‑150 (MQ=255)
                                                   gCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAAccc                                                                                                    <  1:272569/150‑1 (MQ=255)
                                                        gAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGc                                                                                               >  2:1642722/1‑150 (MQ=255)
                                                         aaTATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCa                                                                                              <  2:766716/150‑1 (MQ=255)
                                                          atatTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAg                                                                                             >  2:3824394/1‑150 (MQ=255)
                                                          atatTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAg                                                                                             >  2:659/1‑150 (MQ=255)
                                                           tatTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGc                                                                                            >  2:2127638/1‑150 (MQ=255)
                                                            atTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCt                                                                                           <  2:1339499/150‑1 (MQ=255)
                                                            atTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCt                                                                                           >  1:79259/1‑150 (MQ=255)
                                                               gAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGc                                                                                        <  2:4125412/150‑1 (MQ=255)
                                                                   gacacCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATcc                                                                                    >  2:2342834/2‑150 (MQ=255)
                                                                     cacCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAg                                                                                  <  1:2758487/150‑1 (MQ=255)
                                                                       ccATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTc                                                                                >  1:3631819/1‑150 (MQ=255)
                                                                       ccATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTc                                                                                <  2:2635710/150‑1 (MQ=255)
                                                                         aTCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGt                                                                              >  1:1092185/1‑150 (MQ=255)
                                                                          tCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGtt                                                                             <  2:2447218/150‑1 (MQ=255)
                                                                           cAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTc                                                                            <  1:2021370/150‑1 (MQ=255)
                                                                           cAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTc                                                                            <  2:1940921/150‑1 (MQ=255)
                                                                            aGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTca                                                                           <  2:735213/150‑1 (MQ=255)
                                                                            aGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTca                                                                           >  2:299359/1‑150 (MQ=255)
                                                                                tCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCacaca                                                                       >  1:2463674/1‑149 (MQ=255)
                                                                                  aCCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACActct                                                                     >  1:1683547/1‑150 (MQ=255)
                                                                                   cccATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCtt                                                                    <  1:4074218/150‑1 (MQ=255)
                                                                                     cATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTcc                                                                  <  1:3545478/150‑1 (MQ=255)
                                                                                     cATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTcc                                                                  >  2:3103013/1‑150 (MQ=255)
                                                                                     cATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTcc                                                                  <  1:1482582/150‑1 (MQ=255)
                                                                                        ttCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGgt                                                               >  1:1244006/1‑150 (MQ=255)
                                                                                             atcatcCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGtata                                                          >  1:2342391/1‑150 (MQ=255)
                                                                                              tcatcCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATaa                                                         <  1:3074095/150‑1 (MQ=255)
                                                                                              tcatcCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATaa                                                         >  1:1652354/1‑150 (MQ=255)
                                                                                                 tcCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATa                                                      <  2:3589200/150‑1 (MQ=255)
                                                                                                   cAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACACATACGGAGTATAAATACg                                                    >  1:4154357/1‑150 (MQ=255)
                                                                                                       tCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATg                                                <  1:3038479/150‑1 (MQ=255)
                                                                                                       tCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATg                                                <  2:633726/150‑1 (MQ=255)
                                                                                                        cGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATgg                                               <  2:917495/150‑1 (MQ=255)
                                                                                                          aaCTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGgag                                             >  2:3953622/1‑150 (MQ=255)
                                                                                                           aCTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACACTTACGGTGTATAAATACGCATGgaga                                            >  1:2234312/1‑150 (MQ=255)
                                                                                                            ctctGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGgagag                                           <  1:1420460/150‑1 (MQ=255)
                                                                                                                gCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGa                                       <  2:1544628/150‑1 (MQ=255)
                                                                                                                gCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGa                                       <  2:1544654/150‑1 (MQ=255)
                                                                                                                 cGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGAt                                      <  2:1953755/150‑1 (MQ=255)
                                                                                                                  gTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGTGGCTc                                     >  1:1545285/1‑150 (MQ=255)
                                                                                                                     ctCCAGCGCGAGCACTCGCTCTATCACCATTTCCCGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGTCATCCAGGCGTTCACACTCTTCCGGTGTCTAAATACGCATGGAGTGAGGATCCGa                                  <  1:2106319/149‑1 (MQ=255)
                                                                                                                       ccAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAgg                                <  1:1804244/150‑1 (MQ=255)
                                                                                                                         aGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGcc                              <  1:4026370/150‑1 (MQ=255)
                                                                                                                              gAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTg                         >  2:1429171/1‑150 (MQ=255)
                                                                                                                               aGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGc                        >  1:4109488/1‑150 (MQ=255)
                                                                                                                               aGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGc                        >  1:250512/1‑150 (MQ=255)
                                                                                                                                 cACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCAt                      >  2:378775/1‑150 (MQ=255)
                                                                                                                                 cACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCAt                      >  2:964496/1‑150 (MQ=255)
                                                                                                                                   cTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCg                    <  2:3267511/150‑1 (MQ=255)
                                                                                                                                   cTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTACGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCg                    >  2:330459/1‑150 (MQ=255)
                                                                                                                                    tCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCgg                   <  2:1358757/150‑1 (MQ=255)
                                                                                                                                     cGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGt                  >  2:2204490/1‑150 (MQ=255)
                                                                                                                                     cGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGt                  >  2:1669590/1‑150 (MQ=255)
                                                                                                                                       ctctATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTc                <  1:680958/150‑1 (MQ=255)
                                                                                                                                       ctctATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTc                >  1:1207314/1‑150 (MQ=255)
                                                                                                                                       ctctATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTc                <  1:680994/150‑1 (MQ=255)
                                                                                                                                         ctATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTg              >  1:2914535/1‑150 (MQ=255)
                                                                                                                                          tATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGc             <  2:3869134/150‑1 (MQ=255)
                                                                                                                                           aTCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGcc            >  2:3699853/1‑150 (MQ=255)
                                                                                                                                           aTCACCATTTAACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACACTTACGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGcc            >  1:3440771/1‑150 (MQ=255)
                                                                                                                                            tCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCa           <  1:3802203/150‑1 (MQ=255)
                                                                                                                                            tCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCa           <  1:2808774/150‑1 (MQ=255)
                                                                                                                                            tCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCa           <  1:2293195/150‑1 (MQ=255)
                                                                                                                                                 aTTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAAcccc      <  2:3650069/150‑2 (MQ=255)
                                                                                                                                                  tttCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCtt     <  1:540581/150‑1 (MQ=255)
                                                                                                                                                  tttCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCtt     <  2:770265/150‑1 (MQ=255)
                                                                                                                                                   ttCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTc    <  1:2885618/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCT  >  CP009273/3425365‑3425663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑