Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,440,151 G→T 100% I31I (ATC→ATA)  rpsN ← 30S ribosomal subunit protein S14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,440,1510GT99.2% 910.4 / ‑1.6 262I31I (ATC→ATA) rpsN30S ribosomal subunit protein S14
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (114/144);  total (118/144)
Fisher's exact test for biased strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 272 total aligned reads displayed.

GCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCG  >  CP009273/3440004‑3440297
                                                                                                                                                   |                                                                                                                                                  
 cTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAtatg                                                                                                                                                 <  2:3732416/150‑1 (MQ=255)
  tCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCAGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAtatga                                                                                                                                                >  2:1908906/1‑150 (MQ=255)
   cAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAtatgat                                                                                                                                               >  2:602267/1‑150 (MQ=255)
    aaCCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATc                                                                                                                                              >  1:2052083/1‑150 (MQ=255)
    aaCCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATc                                                                                                                                              >  2:1042927/1‑150 (MQ=255)
    aaCCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATc                                                                                                                                              <  2:791020/150‑1 (MQ=255)
    aaCCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATc                                                                                                                                              <  2:1625309/150‑1 (MQ=255)
      cccGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGAAGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTACGTCGGAAGCGTTCACTTCAGAAATGATCGc                                                                                                                                            >  2:1428707/1‑150 (MQ=255)
        cGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATAGGATCGCtt                                                                                                                                          <  2:2353422/150‑1 (MQ=255)
         gAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGTTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCttt                                                                                                                                         <  1:4018146/150‑1 (MQ=255)
         gAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCttt                                                                                                                                         <  2:2566151/150‑1 (MQ=255)
         gAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCttt                                                                                                                                         <  2:16961/150‑1 (MQ=255)
          aaCTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTttc                                                                                                                                        <  2:939597/150‑1 (MQ=255)
          aaCTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTttc                                                                                                                                        >  1:2207869/1‑150 (MQ=255)
                cGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTttcagttca                                                                                                                                  <  2:1472267/150‑1 (MQ=255)
                cGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTttcagttca                                                                                                                                  <  1:187849/150‑1 (MQ=255)
                 gCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTttcagttcag                                                                                                                                 >  1:289545/1‑150 (MQ=255)
                     gAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGcgcg                                                                                                                             <  2:135350/150‑1 (MQ=255)
                         ccATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTc                                                                                                                         >  1:2291367/1‑150 (MQ=255)
                         ccATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTc                                                                                                                         <  2:352311/150‑1 (MQ=255)
                          cATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTcg                                                                                                                        <  1:2617950/150‑1 (MQ=255)
                           aTGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTctc                                                                                                                       >  1:2129595/1‑148 (MQ=255)
                            tGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTcgcg                                                                                                                      <  1:3032177/150‑1 (MQ=255)
                            tGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTcgcg                                                                                                                      >  1:1588723/1‑150 (MQ=255)
                               ggACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAg                                                                                                                   >  1:81927/1‑150 (MQ=255)
                               ggACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAg                                                                                                                   >  1:2097071/1‑150 (MQ=255)
                                 acgacCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTa                                                                                                                 >  1:237253/1‑150 (MQ=255)
                                 acgacCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTa                                                                                                                 >  1:1083021/1‑150 (MQ=255)
                                    acCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTAttt                                                                                                              >  1:3941788/1‑150 (MQ=255)
                                    acCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTAttt                                                                                                              <  1:258601/150‑1 (MQ=255)
                                    acCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTAttt                                                                                                              >  2:3606671/1‑150 (MQ=255)
                                          ttGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGc                                                                                                        <  2:2256478/150‑1 (MQ=255)
                                            gACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTa                                                                                                      <  1:1515817/150‑1 (MQ=255)
                                                gCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGc                                                                                                  <  1:2033741/150‑1 (MQ=255)
                                                 cAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCt                                                                                                 >  1:239370/1‑150 (MQ=255)
                                                 cAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCt                                                                                                 >  2:2130209/1‑150 (MQ=255)
                                                 cAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCt                                                                                                 >  2:2245647/1‑150 (MQ=255)
                                                  aGCGGTGACGCTGACGAGACGGGCTGGAATCACGCGACAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTa                                                                                                <  2:1256993/150‑1 (MQ=255)
                                                    cGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTAcg                                                                                              <  1:2902567/150‑1 (MQ=255)
                                                     ggTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTAcgc                                                                                             >  1:139530/1‑150 (MQ=255)
                                                       ttACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGt                                                                                           >  1:1248403/1‑150 (MQ=255)
                                                         aCGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGttt                                                                                         <  1:168638/150‑1 (MQ=255)
                                                          cGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGtttt                                                                                        <  2:339134/150‑1 (MQ=255)
                                                           gCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTa                                                                                       <  1:2418311/150‑1 (MQ=255)
                                                               aCGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTc                                                                                   <  2:2017846/150‑1 (MQ=255)
                                                                cGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTcg                                                                                  >  2:1062982/1‑150 (MQ=255)
                                                                cGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTcg                                                                                  <  1:1376383/150‑1 (MQ=255)
                                                                 gagaCGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTcgc                                                                                 >  2:126379/1‑150 (MQ=255)
                                                                  agaCGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTcgcg                                                                                >  1:499379/1‑150 (MQ=255)
                                                                  agaCGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTcgcg                                                                                >  1:3408589/1‑150 (MQ=255)
                                                                      gggCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCt                                                                            >  1:348375/1‑150 (MQ=255)
                                                                          tGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCa                                                                        <  1:4068686/150‑1 (MQ=255)
                                                                          tGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCa                                                                        <  1:2511813/150‑1 (MQ=255)
                                                                           ggAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCat                                                                       >  1:1304937/1‑150 (MQ=255)
                                                                            gAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCatt                                                                      >  2:1140003/1‑150 (MQ=255)
                                                                             aaTCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCattg                                                                     >  2:3734020/1‑150 (MQ=255)
                                                                             aaTCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCattg                                                                     <  1:4052208/150‑1 (MQ=255)
                                                                             aaTCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCattg                                                                     <  1:2036876/150‑1 (MQ=255)
                                                                              aTCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCattga                                                                    <  1:1017275/150‑1 (MQ=255)
                                                                               tCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCTTGCTTTCattgat                                                                   <  2:3721558/150‑1 (MQ=255)
                                                                               tCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCattgat                                                                   <  1:3995237/150‑1 (MQ=255)
                                                                                 aCGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACCTCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCattgattg                                                                 <  1:1238280/150‑1 (MQ=255)
                                                                                      gCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAg                                                            <  2:435914/150‑1 (MQ=255)
                                                                                       cAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGc                                                           <  1:1009662/150‑1 (MQ=255)
                                                                                       cAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGc                                                           <  1:1642051/150‑1 (MQ=255)
                                                                                        agagTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGcc                                                          >  1:4152773/1‑150 (MQ=255)
                                                                                          agTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAt                                                        <  1:3206921/150‑1 (MQ=255)
                                                                                           gTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAtt                                                       <  1:3518337/150‑1 (MQ=255)
                                                                                             cTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTa                                                     <  1:3796206/150‑1 (MQ=255)
                                                                                             cTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTa                                                     <  1:952155/150‑1 (MQ=255)
                                                                                              tGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTANCCATTTAg                                                    <  1:3661135/150‑1 (MQ=255)
                                                                                                cAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTa                                                  >  1:3260172/1‑150 (MQ=255)
                                                                                                cAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTa                                                  >  1:1740405/1‑150 (MQ=255)
                                                                                                  tcTTGAGAACAGATTTCCTACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTGATCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACTCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCTTTTAGTTAc                                                <  2:2185923/149‑1 (MQ=255)
                                                                                                      gagaACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTa                                            >  2:2747891/1‑150 (MQ=255)
                                                                                                        gaACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTAcc                                          <  1:2732981/150‑1 (MQ=255)
                                                                                                            aGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTAc                                      <  2:3323387/150‑1 (MQ=255)
                                                                                                             gCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACt                                     <  1:1625644/150‑1 (MQ=255)
                                                                                                             gCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACt                                     <  2:3188342/150‑1 (MQ=255)
                                                                                                             gCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACt                                     <  2:3188185/150‑1 (MQ=255)
                                                                                                               gTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTg                                   >  1:3715596/1‑150 (MQ=255)
                                                                                                               gTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTg                                   <  2:2442445/150‑1 (MQ=255)
                                                                                                                  ccAACGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCgg                                >  2:1420409/1‑150 (MQ=255)
                                                                                                                      cGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACg                            >  2:2814574/1‑150 (MQ=255)
                                                                                                                      cGATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACg                            <  1:687726/150‑1 (MQ=255)
                                                                                                                       gATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACgg                           >  1:3109278/1‑150 (MQ=255)
                                                                                                                       gATCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACgg                           >  2:1110242/1‑150 (MQ=255)
                                                                                                                        aTCTTCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACggg                          >  1:2725028/1‑150 (MQ=255)
                                                                                                                           ttCGTCGGAAGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAg                       <  2:2754915/150‑1 (MQ=255)
                                                                                                                             cgtcgGAAGCGTTCACATCAGCTATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTc                     <  1:1784997/150‑1 (MQ=255)
                                                                                                                                   aaGCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGc               <  2:3092693/150‑1 (MQ=255)
                                                                                                                                     gCGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcag             <  2:4107019/150‑1 (MQ=255)
                                                                                                                                      cGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagc            <  2:254148/150‑1 (MQ=255)
                                                                                                                                      cGTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagc            >  2:4044836/1‑150 (MQ=255)
                                                                                                                                       gTTCACATCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagcc           <  2:2976749/150‑1 (MQ=255)
                                                                                                                                           acaTCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCcagca       >  2:1925636/1‑150 (MQ=255)
                                                                                                                                           acaTCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCcagca       <  1:530527/150‑1 (MQ=255)
                                                                                                                                            caTCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCcagcag      <  1:1210667/150‑1 (MQ=255)
                                                                                                                                             aTCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCaga     >  2:1212358/1‑150 (MQ=255)
                                                                                                                                              tCAGATATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCagag    <  2:1733534/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCG  >  CP009273/3440004‑3440297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑