Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,501,571 C→A 100% G127C (GGC→TGC)  php ← phosphotriesterase homology protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,501,5710CA97.0% 777.1 / 12.8 232G127C (GGC→TGC) phpphosphotriesterase homology protein
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base A (95/130);  total (97/135)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 232 total aligned reads displayed.

GTGGGCTTGTAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCA  >  CP009273/3501425‑3501717
                                                                                                                                                  |                                                                                                                                                  
gTGGGCTTGTAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCTGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGAt                                                                                                                                                 <  1:3807902/150‑1 (MQ=255)
  gggCTTGTAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGAtct                                                                                                                                               <  1:2315051/150‑1 (MQ=255)
    gCTTGTAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTcc                                                                                                                                             <  1:2174861/150‑1 (MQ=255)
       tGTAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCcgcg                                                                                                                                          >  2:720443/1‑150 (MQ=255)
       tGTAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCcgcg                                                                                                                                          <  2:2774427/150‑1 (MQ=255)
       tGTAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCcgcg                                                                                                                                          >  1:2801182/1‑150 (MQ=255)
         tAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCgat                                                                                                                                        <  1:1336946/150‑1 (MQ=255)
            cAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCgatgat                                                                                                                                     <  2:2482291/150‑1 (MQ=255)
             aaCGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATc                                                                                                                                    >  1:1164878/1‑150 (MQ=255)
                  cAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGc                                                                                                                               <  2:475569/150‑1 (MQ=255)
                   aGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCt                                                                                                                              <  1:3873947/150‑1 (MQ=255)
                    gTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCtt                                                                                                                             <  2:586461/150‑1 (MQ=255)
                      tGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTc                                                                                                                           <  1:1152737/150‑1 (MQ=255)
                      tGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTc                                                                                                                           <  1:3411484/150‑1 (MQ=255)
                       gCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCa                                                                                                                          >  1:1886885/1‑150 (MQ=255)
                       gCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCa                                                                                                                          <  1:1737996/150‑1 (MQ=255)
                        cTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGTTTTCAg                                                                                                                         <  2:2381371/150‑1 (MQ=255)
                        cTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAg                                                                                                                         <  1:3078321/150‑1 (MQ=255)
                        cTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAg                                                                                                                         >  2:1717374/1‑150 (MQ=255)
                           cAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTc                                                                                                                      <  2:2397901/150‑1 (MQ=255)
                            aggcccATCGTGCTGATCGACGTATGGGTGGAGATCGGGCGTCCGGTCGGGTTATGCTCCAGGGCAGCGGCTTTTTCTTACTTCTCTTCGTGCGGCTTTATCTTTCCTTGGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCTGCTcc                                                                                                                     <  2:3492252/147‑1 (MQ=255)
                            aGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTcc                                                                                                                     <  2:3295841/150‑1 (MQ=255)
                            aGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTcc                                                                                                                     >  1:4038523/1‑150 (MQ=255)
                            aGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTcc                                                                                                                     <  1:3341571/150‑1 (MQ=255)
                            aGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTcc                                                                                                                     <  1:3687937/150‑1 (MQ=255)
                             gCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCg                                                                                                                    <  1:494021/150‑1 (MQ=255)
                              ccccATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGt                                                                                                                   >  2:1399528/1‑150 (MQ=255)
                                ccATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGc                                                                                                                 <  2:2317934/150‑1 (MQ=255)
                                ccATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGc                                                                                                                 <  2:1406494/150‑1 (MQ=255)
                                 cATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGcc                                                                                                                >  1:3559078/1‑150 (MQ=255)
                                 cATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGcc                                                                                                                <  1:796952/150‑1 (MQ=255)
                                    cGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATc                                                                                                             >  1:1642229/1‑150 (MQ=255)
                                     gTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCg                                                                                                            <  1:4163694/150‑1 (MQ=255)
                                      tGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGa                                                                                                           >  2:4151580/1‑150 (MQ=255)
                                           aaCGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATAccc                                                                                                      <  2:1868925/150‑1 (MQ=255)
                                            acgacgTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGATCCGAGCCATCGATACCCt                                                                                                     >  1:851871/1‑150 (MQ=255)
                                                 gTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCa                                                                                                <  2:3200242/150‑1 (MQ=255)
                                                 gTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCa                                                                                                >  2:3301416/1‑150 (MQ=255)
                                                  tATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCaa                                                                                               >  2:2214356/1‑150 (MQ=255)
                                                   aTGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAAt                                                                                              >  2:1064544/1‑150 (MQ=255)
                                                    tGCGTGGAGATCGGGCGTCCGGTCTGTTTATGCGCCAGAGCAGCGGCAATTAATACCTTCTATTCCAGCGTCGTAATCTTTCATTCGGTGGTGCAGATCTCCGCGATGATCCAGGCTTTCAGCTCCGTGCCATCGATTCCCTGTTCAAtt                                                                                             <  1:3131160/150‑1 (MQ=255)
                                                        tGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCAt                                                                                         <  1:2866553/150‑1 (MQ=255)
                                                         ggAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATc                                                                                        <  1:1295094/150‑1 (MQ=255)
                                                          gagaTCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCg                                                                                       <  2:293012/150‑1 (MQ=255)
                                                          gagaTCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCg                                                                                       <  2:380401/150‑1 (MQ=255)
                                                          gagaTCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCg                                                                                       >  2:2503747/1‑150 (MQ=255)
                                                              tCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCa                                                                                   <  2:176450/150‑1 (MQ=255)
                                                              tCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCa                                                                                   >  1:2038994/1‑150 (MQ=255)
                                                                  gCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCAtctc                                                                               <  2:1544629/150‑1 (MQ=255)
                                                                  gCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCAtctc                                                                               <  2:3778602/150‑1 (MQ=255)
                                                                  gCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCAtctc                                                                               <  1:1950311/150‑1 (MQ=255)
                                                                    gTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATATTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCt                                                                             >  1:2676420/1‑150 (MQ=255)
                                                                     tCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTg                                                                            <  2:1123619/150‑1 (MQ=255)
                                                                     tCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTg                                                                            >  2:3922264/1‑150 (MQ=255)
                                                                            tGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGt                                                                     >  2:3388756/1‑150 (MQ=255)
                                                                             ggTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGtt                                                                    >  2:1707944/1‑150 (MQ=255)
                                                                              gTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTc                                                                   >  2:3572933/1‑150 (MQ=255)
                                                                               ttATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATCCCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTcc                                                                  >  2:386530/1‑150 (MQ=255)
                                                                               ttATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTcc                                                                  <  2:3597973/150‑1 (MQ=255)
                                                                                     gcCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACg                                                            <  2:4017700/150‑1 (MQ=255)
                                                                                          cgcagcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCg                                                       <  1:623993/150‑1 (MQ=255)
                                                                                             agcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCCTCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGt                                                    <  2:622296/150‑1 (MQ=255)
                                                                                             agcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGt                                                    >  1:1093309/1‑150 (MQ=255)
                                                                                             agcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGt                                                    >  2:2362575/1‑150 (MQ=255)
                                                                                             agcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGt                                                    >  2:2491038/1‑150 (MQ=255)
                                                                                             agcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGt                                                    >  2:2491074/1‑150 (MQ=255)
                                                                                              gcgGCAATAAATACCGTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTc                                                   <  2:3655608/150‑1 (MQ=255)
                                                                                                 gCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGcc                                                <  2:3060035/150‑1 (MQ=255)
                                                                                                   aataaataCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCcac                                              <  2:312875/150‑1 (MQ=255)
                                                                                                   aataaataCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCcac                                              >  2:2015904/1‑150 (MQ=255)
                                                                                                     taaataCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACAt                                            >  2:1958404/1‑150 (MQ=255)
                                                                                                     taaataCCTTCTCTTCCAGCGGCGTAATATGTCCGTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACAt                                            <  1:2647692/150‑1 (MQ=255)
                                                                                                         taCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTc                                        >  2:2287104/1‑150 (MQ=255)
                                                                                                           ccTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCg                                      <  1:774051/150‑1 (MQ=255)
                                                                                                             ttCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCggg                                    >  1:3276585/1‑150 (MQ=255)
                                                                                                             ttCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCggg                                    >  2:663945/1‑150 (MQ=255)
                                                                                                               ctctTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGaa                                  <  2:314080/150‑1 (MQ=255)
                                                                                                                 ctTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGaaaa                                >  1:1813091/1‑150 (MQ=255)
                                                                                                                   tCCAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAAc                              >  2:1944275/1‑150 (MQ=255)
                                                                                                                    ccAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAAcg                             <  1:90423/150‑1 (MQ=255)
                                                                                                                    ccAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAAcg                             >  2:1942000/1‑150 (MQ=255)
                                                                                                                    ccAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAAcg                             <  1:418101/150‑1 (MQ=255)
                                                                                                                     cAGCGGCGTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAAcgc                            >  1:390849/1‑150 (MQ=255)
                                                                                                                       gcggcgTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGt                          <  1:3507298/150‑1 (MQ=255)
                                                                                                                        cggcgTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTc                         <  1:3158467/150‑1 (MQ=255)
                                                                                                                         ggcgTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTcc                        <  1:598512/150‑1 (MQ=255)
                                                                                                                          gcgTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCt                       <  2:4102215/150‑1 (MQ=255)
                                                                                                                           cgTAATCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTg                      <  1:1219209/150‑1 (MQ=255)
                                                                                                                              aaTCTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGta                   >  2:1399894/1‑150 (MQ=255)
                                                                                                                                 cTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGtaata                >  2:347624/1‑150 (MQ=255)
                                                                                                                                 cTTTCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGtaata                <  1:1497459/150‑1 (MQ=255)
                                                                                                                                   ttCCTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAAc              >  2:1845858/1‑150 (MQ=255)
                                                                                                                                     ccTTCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCg            <  2:2947498/150‑1 (MQ=255)
                                                                                                                                       ttCGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGt          <  1:2336865/150‑1 (MQ=255)
                                                                                                                                         cGCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTAc        <  2:93890/150‑1 (MQ=255)
                                                                                                                                          gCTGGTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACa       <  2:2520559/150‑1 (MQ=255)
                                                                                                                                            tGGTGAAGATCTCCGAGATGAACCCGGCTTTCAGCTCCGTGCCATCGATACCCGGTTCAATTTCAGCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTCATAACCGGTACAgg     >  2:495213/1‑150 (MQ=255)
                                                                                                                                             ggTGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGc    >  2:292341/1‑150 (MQ=255)
                                                                                                                                              gTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGcc   <  2:771180/150‑1 (MQ=255)
                                                                                                                                               tGCAGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGcca  <  2:524844/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTGGGCTTGTAGCAACGCCAGTTGCTCCAGCCCCATCGTGCTGAACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCA  >  CP009273/3501425‑3501717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑