Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,544,521 G→T 24.8% H438N (CAC→AAC)  malP ← maltodextrin phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,544,5210GT24.8% 644.3 / 168.1 258H438N (CAC→AAC) malPmaltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (120/73);  new base T (35/29);  total (156/102)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

CAGATTGATCAGCTGATCGAGATCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTTTTCATCGCCCG  >  CP009273/3544372‑3544670
                                                                                                                                                     |                                                                                                                                                     
cAGATTGATCAGCTGATCGAGATCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTg                                                                                                                                                       >  1:2310593/1‑150 (MQ=255)
 aGATTGATCAGCTGATCGAGATCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGa                                                                                                                                                      >  1:333730/1‑150 (MQ=255)
     tGATCAGCTGATCGAGATCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATAtt                                                                                                                                                  <  2:1003512/150‑1 (MQ=255)
       aTCAGCTGATCGAGATCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTcc                                                                                                                                                <  1:2128474/150‑1 (MQ=255)
       aTCAGCTGATCGAGATCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTcc                                                                                                                                                <  1:2767484/150‑1 (MQ=255)
       aTCAGCTGATCGAGATCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTcc                                                                                                                                                >  2:4046775/1‑150 (MQ=255)
        tCAGCTGATCGAGATCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCg                                                                                                                                               <  1:4090297/150‑1 (MQ=255)
        tCAGCTGATCGAGATCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCg                                                                                                                                               <  1:4089782/150‑1 (MQ=255)
         cAGCTGATCGAGATCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCgg                                                                                                                                              >  2:3889579/1‑150 (MQ=255)
          aGCTGATCGAGATCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCggg                                                                                                                                             >  1:2586933/1‑150 (MQ=255)
             tGATCGAGATCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAAc                                                                                                                                          >  2:2041218/1‑150 (MQ=255)
              gATCGAGATCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACa                                                                                                                                         >  1:125496/1‑150 (MQ=255)
                  gagaTCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATc                                                                                                                                     >  1:3018966/1‑150 (MQ=255)
                    gaTCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCtt                                                                                                                                   <  2:659242/150‑1 (MQ=255)
                    gaTCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCtt                                                                                                                                   <  2:2867173/150‑1 (MQ=255)
                    gaTCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCtt                                                                                                                                   <  1:2845935/150‑1 (MQ=255)
                     aTCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCttt                                                                                                                                  <  1:1072453/150‑1 (MQ=255)
                       cGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTca                                                                                                                                >  2:3711373/1‑150 (MQ=255)
                       cGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTca                                                                                                                                >  1:2921825/1‑150 (MQ=255)
                         ttAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTcacc                                                                                                                              >  1:2513863/1‑150 (MQ=255)
                           aGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTcaccac                                                                                                                            >  1:3257194/1‑150 (MQ=255)
                           aGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTcaccac                                                                                                                            >  2:1806264/1‑150 (MQ=255)
                           aGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAAGTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTcaccac                                                                                                                            >  1:551201/1‑150 (MQ=255)
                              ccACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAg                                                                                                                         >  1:947845/1‑150 (MQ=255)
                                 ctctTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATc                                                                                                                      >  2:2324690/1‑150 (MQ=255)
                                     ttttGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAg                                                                                                                  >  2:623454/1‑150 (MQ=255)
                                       ttGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTg                                                                                                                >  1:3642439/1‑150 (MQ=255)
                                           aGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAgc                                                                                                            >  2:208154/1‑150 (MQ=255)
                                             tGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAgcgc                                                                                                          >  1:1580967/1‑150 (MQ=255)
                                              gATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGcgcc                                                                                                         >  1:37083/1‑150 (MQ=255)
                                               aTTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGcgccg                                                                                                        <  1:3940696/150‑1 (MQ=255)
                                               aTTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGcgccg                                                                                                        <  2:3584369/150‑1 (MQ=255)
                                                 ttATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCa                                                                                                      <  1:2643426/150‑1 (MQ=255)
                                                   aTCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAac                                                                                                    >  2:3050493/1‑150 (MQ=255)
                                                      cAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACAcc                                                                                                 <  2:3528214/150‑1 (MQ=255)
                                                       aaCAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCg                                                                                                >  2:1310062/1‑150 (MQ=255)
                                                          agagCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTc                                                                                             >  1:1428273/1‑150 (MQ=255)
                                                          agagCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTc                                                                                             >  2:3208338/1‑150 (MQ=255)
                                                           gagCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCa                                                                                            >  1:2993569/1‑150 (MQ=255)
                                                           gagCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCa                                                                                            >  1:2199587/1‑150 (MQ=255)
                                                            agCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGACTTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCAc                                                                                           >  2:3688940/1‑150 (MQ=255)
                                                             gccgccAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCAcc                                                                                          <  1:2540739/150‑1 (MQ=255)
                                                               cgccAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACcgc                                                                                        >  1:1891450/1‑150 (MQ=255)
                                                               cgccAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACcgc                                                                                        >  2:1896659/1‑150 (MQ=255)
                                                                gccAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACcgcg                                                                                       >  2:3988192/1‑150 (MQ=255)
                                                                 ccAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGa                                                                                      >  1:3226220/1‑150 (MQ=255)
                                                                   aGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGaaa                                                                                    >  2:277721/1‑150 (MQ=255)
                                                                     tGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAAcc                                                                                  <  1:160519/150‑1 (MQ=255)
                                                                       ccGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGc                                                                                >  2:1943107/1‑150 (MQ=255)
                                                                        cGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGACTTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGcc                                                                               >  2:3366328/1‑150 (MQ=255)
                                                                        cGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGcc                                                                               >  2:3366346/1‑150 (MQ=255)
                                                                         gggTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCa                                                                              <  1:1514823/150‑1 (MQ=255)
                                                                         gggTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCa                                                                              >  2:3608134/1‑150 (MQ=255)
                                                                          ggTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCaa                                                                             >  1:3069106/1‑150 (MQ=255)
                                                                             tGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACca                                                                          >  1:1655045/1‑150 (MQ=255)
                                                                              gCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACcac                                                                         <  1:1028318/150‑1 (MQ=255)
                                                                                aCTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACcacac                                                                       <  1:439062/150‑1 (MQ=255)
                                                                                 cTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACcacaca                                                                      >  2:2367138/1‑150 (MQ=255)
                                                                                   gTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACcacacaca                                                                    >  2:777767/1‑150 (MQ=255)
                                                                                   gTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACcacacaca                                                                    <  2:3902333/150‑1 (MQ=255)
                                                                                   gTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACcacacaca                                                                    <  2:1818628/150‑1 (MQ=255)
                                                                                        aTCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTc                                                               >  1:1182039/1‑150 (MQ=255)
                                                                                          ccAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGc                                                             >  1:653405/1‑150 (MQ=255)
                                                                                             gCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCat                                                          <  2:3524427/150‑1 (MQ=255)
                                                                                              cGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCata                                                         >  1:1218947/1‑150 (MQ=255)
                                                                                                   tGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCAc                                                    <  2:263967/150‑1 (MQ=255)
                                                                                                     gggTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACtt                                                  <  2:3291694/150‑1 (MQ=255)
                                                                                                      ggTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACttg                                                 >  1:3190036/1‑150 (MQ=255)
                                                                                                        tAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACttgtt                                               <  1:836599/150‑1 (MQ=255)
                                                                                                             ccGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCg                                          <  1:1702894/150‑1 (MQ=255)
                                                                                                              cGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGt                                         >  2:2728569/1‑150 (MQ=255)
                                                                                                               tttGGTGACTTTAATTAAATTGATCGGCCATAGCTGGTCAAAAAACGTGAAAACATCTATCACCACAATATCCGAGAGAAGAGCCTCAACACCATTAACCGCTAAACCGCCAACCACACACAGGATCGCCATATTCACTTGTTTGTCgag                                        >  2:934958/2‑148 (MQ=255)
                                                                                                               gTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTg                                        >  1:3786062/1‑150 (MQ=255)
                                                                                                                     gACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGcaccac                                  >  2:1259300/1‑150 (MQ=255)
                                                                                                                        gTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGc                               >  1:2436419/1‑150 (MQ=255)
                                                                                                                        gTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGc                               >  1:2437854/1‑150 (MQ=255)
                                                                                                                        gTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGc                               >  1:3396070/1‑150 (MQ=255)
                                                                                                                           aTGGAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAg                            >  2:3029679/1‑150 (MQ=255)
                                                                                                                              gAATTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGttt                         <  1:3405453/150‑1 (MQ=255)
                                                                                                                               aaTTTGTTCGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTg                        >  2:2948955/1‑150 (MQ=255)
                                                                                                                               aaTTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTg                        <  1:2237937/150‑1 (MQ=255)
                                                                                                                                aTTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTgg                       >  2:1165895/1‑150 (MQ=255)
                                                                                                                                    gTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCca                   >  1:2004201/1‑150 (MQ=255)
                                                                                                                                    gTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCca                   >  2:2047515/1‑150 (MQ=255)
                                                                                                                                       cGGCCATAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCacac                <  2:172758/150‑1 (MQ=255)
                                                                                                                                       cGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCacac                >  2:282426/1‑150 (MQ=255)
                                                                                                                                        ggCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACt               <  2:4123791/150‑1 (MQ=255)
                                                                                                                                          ccATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACttt             <  2:1074845/150‑1 (MQ=255)
                                                                                                                                          ccATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACttt             >  2:3114107/1‑150 (MQ=255)
                                                                                                                                             tAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTTTTc          >  1:3909854/1‑150 (MQ=255)
                                                                                                                                             tAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTTTTc          >  1:2963144/1‑150 (MQ=255)
                                                                                                                                             tAGCTGGTTATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTTTTc          >  2:4083874/1‑150 (MQ=255)
                                                                                                                                             tAGCTGGTGATAGTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTTTTc          <  2:150735/150‑1 (MQ=255)
                                                                                                                                               gCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTTTTCAt        <  1:341877/150‑1 (MQ=255)
                                                                                                                                               gCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTTTTCAt        <  1:3328608/150‑1 (MQ=255)
                                                                                                                                                 tGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATGTGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTTTTCATCg      >  2:119019/1‑150 (MQ=255)
                                                                                                                                                   gTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTTTTCATCGcc    >  1:898901/1‑150 (MQ=255)
                                                                                                                                                    tGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTTTTCATCGccc   <  2:2205215/150‑1 (MQ=255)
                                                                                                                                                     gATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTTTTCATCGCCCg  >  2:436907/1‑150 (MQ=255)
                                                                                                                                                     gATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTTTTCATCGCCCg  <  1:1863319/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGATTGATCAGCTGATCGAGATCGTTAGCCCACTCTTTTTGCAGTGATTTATCCAACAGAGCCGCCAGTGCCGGGTTGCACTGTTTGATCCAGCGACGTGGGGTAATACCGTTGGTGACGTTATGGAATTTGTTCGGCCATAGCTGGTGATATTCCGGGAACAGATCTTTCACCACCAGATCCGAGTGCAGCGCCGCAACACCGTTCACCGCGAAACCGCCAACCACACACAGGTTCGCCATATGCACTTGTTTGTCGTGCACCACCGCCAGTTTGGCCCACACTTTTTCATCGCCCG  >  CP009273/3544372‑3544670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑