Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,566,544 C→A 5.1% E107* (GAA→TAA)  glgB ← 1,4‑alpha‑glucan branching enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,566,5440CA 5.1% 960.1 / 18.7 244E107* (GAA→TAA) glgB1,4‑alpha‑glucan branching enzyme
Reads supporting (aligned to +/- strand):  ref base C (94/137);  new base A (4/9);  total (98/146)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

ATTGCCCAACCACCGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGACACTCCAGTTTTGCG  >  CP009273/3566397‑3566692
                                                                                                                                                   |                                                                                                                                                    
 ttGCCCAACCACCGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTg                                                                                                                                                   <  1:365099/150‑1 (MQ=255)
     ccAACCACCGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGAtt                                                                                                                                               <  1:523261/150‑1 (MQ=255)
     ccAACCACCGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGAtt                                                                                                                                               <  1:1636191/150‑1 (MQ=255)
       aaCCACCGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAg                                                                                                                                             >  1:200292/1‑150 (MQ=255)
        accaccGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGc                                                                                                                                            >  2:3345972/1‑150 (MQ=255)
           accGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCTTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTACTGGATTAGCGGa                                                                                                                                         <  2:3247594/150‑1 (MQ=255)
           accGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCTTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTACTGGATTAGCGGa                                                                                                                                         <  2:3888952/150‑1 (MQ=255)
             cGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGAcc                                                                                                                                       <  2:1934282/150‑1 (MQ=255)
              gagaCCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCa                                                                                                                                      >  1:141291/1‑150 (MQ=255)
              gagaCCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCa                                                                                                                                      <  2:1419213/150‑1 (MQ=255)
                   ccGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACg                                                                                                                                 <  2:3071781/150‑1 (MQ=255)
                     gACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGt                                                                                                                               >  1:3025054/1‑150 (MQ=255)
                       cGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTaa                                                                                                                             <  2:3969202/150‑1 (MQ=255)
                            gTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGatc                                                                                                                        <  2:3584291/150‑1 (MQ=255)
                            gTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGatc                                                                                                                        <  2:3114641/150‑1 (MQ=255)
                               tGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGatcatc                                                                                                                     >  1:3390294/1‑150 (MQ=255)
                               tGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGatcatc                                                                                                                     <  2:2844337/150‑1 (MQ=255)
                                ggAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGAtcatca                                                                                                                    >  2:3494615/1‑150 (MQ=255)
                                ggAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGAtcatca                                                                                                                    >  2:1800130/1‑150 (MQ=255)
                                    cccAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCatcaatca                                                                                                                <  2:294270/150‑1 (MQ=255)
                                     ccAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAg                                                                                                               >  1:1157027/1‑150 (MQ=255)
                                      cagacagaGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAgg                                                                                                              >  2:1492143/1‑150 (MQ=255)
                                      cagacagaGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAgg                                                                                                              <  1:3897371/150‑1 (MQ=255)
                                       agacagaGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGt                                                                                                             <  1:3288195/150‑1 (MQ=255)
                                       agacagaGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGt                                                                                                             >  2:4038100/1‑150 (MQ=255)
                                          cagaGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGtttt                                                                                                          <  1:2548183/150‑1 (MQ=255)
                                          cagaGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGtttt                                                                                                          <  1:1323848/150‑1 (MQ=255)
                                             agaAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCt                                                                                                       >  1:110920/1‑150 (MQ=255)
                                                 aCGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGAcc                                                                                                   >  2:831048/1‑150 (MQ=255)
                                                  cgcgTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCa                                                                                                  >  1:3681940/1‑150 (MQ=255)
                                                  cgcgTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCa                                                                                                  >  1:3481156/1‑150 (MQ=255)
                                                   gcgTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCAt                                                                                                 <  2:214924/150‑1 (MQ=255)
                                                   gcgTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCAt                                                                                                 <  2:2495478/150‑1 (MQ=255)
                                                      tACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGcc                                                                                              <  2:974025/150‑1 (MQ=255)
                                                        ccTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAg                                                                                            >  2:469321/1‑150 (MQ=255)
                                                        ccTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCATGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAg                                                                                            <  1:834353/150‑1 (MQ=255)
                                                          tgtgACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTACTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGac                                                                                          <  1:1986824/150‑1 (MQ=255)
                                                             gACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGacaac                                                                                       >  1:3938294/1‑150 (MQ=255)
                                                             gACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGacaac                                                                                       >  1:2142609/1‑150 (MQ=255)
                                                                gCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGc                                                                                    >  2:1332442/1‑150 (MQ=255)
                                                                      catAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTg                                                                              >  1:4101564/1‑150 (MQ=255)
                                                                        tAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGAt                                                                            <  2:2580240/150‑1 (MQ=255)
                                                                         aGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATa                                                                           >  1:1707924/1‑150 (MQ=255)
                                                                         aGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATa                                                                           <  2:2552568/150‑1 (MQ=255)
                                                                          gTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAg                                                                          <  1:2943416/150‑1 (MQ=255)
                                                                          gTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAg                                                                          <  1:932304/150‑1 (MQ=255)
                                                                             tCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCgg                                                                       <  2:371712/150‑1 (MQ=255)
                                                                             tCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCgg                                                                       <  2:1032308/150‑1 (MQ=255)
                                                                              cTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGa                                                                      <  1:3834321/150‑1 (MQ=255)
                                                                               tGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGaa                                                                     >  1:671859/1‑150 (MQ=255)
                                                                               tGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGaa                                                                     >  1:2783216/1‑150 (MQ=255)
                                                                                  aTGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGaaaaa                                                                  <  2:2317089/150‑1 (MQ=255)
                                                                                     cgcgcCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAAtt                                                               >  2:3516129/1‑150 (MQ=255)
                                                                                      gcgcCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTc                                                              >  1:3563726/1‑150 (MQ=255)
                                                                                         ccTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGTTTTTGCTGACCATGCCAGACAACATCCAACTGATAGCGGAAAAAATTCTTa                                                           >  1:1533906/1‑150 (MQ=255)
                                                                                         ccTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTa                                                           <  1:2384400/150‑1 (MQ=255)
                                                                                         ccTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTa                                                           >  2:1098606/1‑150 (MQ=255)
                                                                                         ccTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTa                                                           <  2:569911/150‑1 (MQ=255)
                                                                                         ccTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTa                                                           <  1:1975236/150‑1 (MQ=255)
                                                                                          cTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTAc                                                          >  2:11196/1‑150 (MQ=255)
                                                                                          cTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTAc                                                          <  1:1276705/150‑1 (MQ=255)
                                                                                           tAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACg                                                         >  1:70431/1‑150 (MQ=255)
                                                                                            aaGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGt                                                        >  2:2947522/1‑150 (MQ=255)
                                                                                               gTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTcgc                                                     >  2:4131803/1‑150 (MQ=255)
                                                                                                tttCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTACTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTcgcg                                                    >  1:1930333/1‑150 (MQ=255)
                                                                                                   cATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGac                                                 >  2:2076605/1‑149 (MQ=255)
                                                                                                    aTACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAAt                                                >  2:304250/1‑150 (MQ=255)
                                                                                                       cGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGAc                                             <  1:1293944/150‑1 (MQ=255)
                                                                                                       cGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGAc                                             >  2:1678956/1‑150 (MQ=255)
                                                                                                          gcgcAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGAcgcc                                          <  2:4067718/150‑1 (MQ=255)
                                                                                                          gcgcAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGAcgcc                                          <  2:576487/150‑1 (MQ=255)
                                                                                                          gcgcAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGAcgcc                                          <  2:1221329/150‑1 (MQ=255)
                                                                                                           cgcAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGAcgccg                                         <  1:3087/150‑1 (MQ=255)
                                                                                                             cAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCt                                       >  1:4150890/1‑150 (MQ=255)
                                                                                                                gTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTaaa                                    <  1:1307835/150‑1 (MQ=255)
                                                                                                                 tGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAg                                   <  1:781277/150‑1 (MQ=255)
                                                                                                                   aGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGaa                                 <  2:1364120/150‑1 (MQ=255)
                                                                                                                   aGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGaa                                 >  1:957812/1‑150 (MQ=255)
                                                                                                                         ttCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCg                           <  1:2230711/150‑1 (MQ=255)
                                                                                                                            aGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGa                        <  2:487335/150‑1 (MQ=255)
                                                                                                                            aGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGa                        >  1:2999372/1‑150 (MQ=255)
                                                                                                                            aGATAATAGCCAGGCATCCATTTACTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGa                        <  1:3164026/150‑1 (MQ=255)
                                                                                                                             gATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAg                       >  2:2802323/1‑150 (MQ=255)
                                                                                                                               taataGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTc                     >  2:3267099/1‑150 (MQ=255)
                                                                                                                                aataGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCg                    >  1:2767244/1‑150 (MQ=255)
                                                                                                                                   aGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCgaga                 <  1:901810/150‑1 (MQ=255)
                                                                                                                                   aGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCgaga                 <  2:2484943/150‑1 (MQ=255)
                                                                                                                                    gCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGac                <  1:1456217/150‑1 (MQ=255)
                                                                                                                                      cAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGacac              >  1:1292578/1‑150 (MQ=255)
                                                                                                                                         gCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGACACTcc           <  1:3974982/150‑1 (MQ=255)
                                                                                                                                         gCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGACACTca           <  2:2904562/150‑2 (MQ=255)
                                                                                                                                          cATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGACACTCCa          >  1:4035288/1‑150 (MQ=255)
                                                                                                                                          cATCCATTTACTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGACACTCCa          <  1:601636/150‑1 (MQ=255)
                                                                                                                                           aTCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGACACTCCAg         >  2:2236279/1‑150 (MQ=255)
                                                                                                                                             ccATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGACACTCCAGtt       >  1:178282/1‑150 (MQ=255)
                                                                                                                                              cATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGACACTCCAGttt      <  1:2081952/150‑1 (MQ=255)
                                                                                                                                               aTTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGACACTCCAGtttt     >  2:3139436/1‑150 (MQ=255)
                                                                                                                                               aTTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGACACTCCAGtttt     <  1:482945/150‑1 (MQ=255)
                                                                                                                                                tttCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGACACTCCAGTTTTg    <  1:1739093/150‑1 (MQ=255)
                                                                                                                                                 ttCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGACACTCCAGTTTTGc   >  1:913220/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATTGCCCAACCACCGAGACCCGACGGGCGTTTGGAGCCCAGACAGAGAAACGCGTACCTGTGACGCCATCCATAGTATCTGCATGCGCGCCTAAGGTTTCATACGGGCGCAGGTGAGTACCTTCAGATAATAGCCAGGCATCCATTTCCTGGATTAGCGGACCAAAACGGTAAGGATCATCAATCAGGTTTTGCTGACCATGCCAGACAACAGCCAACTGATAGCGGAAAAAATTCTTACGTCGCGGAATGACGCCGCTAAAGAATCCCCGTGAGTCGAGACACTCCAGTTTTGCG  >  CP009273/3566397‑3566692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑