Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,646,763 C→A 7.9% pseudogene (173/483 nt) yhiS → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,646,7630CA 7.9% 979.8 / 37.4 260pseudogene (173/483 nt)yhiSpseudogene
Reads supporting (aligned to +/- strand):  ref base C (133/106);  new base A (11/10);  total (144/116)
Fisher's exact test for biased strand distribution p-value = 8.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

TAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGA  >  CP009273/3646614‑3646910
                                                                                                                                                     |                                                                                                                                                   
tAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGc                                                                                                                                                     >  2:490407/1‑150 (MQ=255)
 aaaGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGcc                                                                                                                                                    <  1:2394672/150‑1 (MQ=255)
   aGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAg                                                                                                                                                  <  1:3151808/150‑1 (MQ=255)
       cacCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATa                                                                                                                                              <  1:3096473/150‑1 (MQ=255)
          ccTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAAcc                                                                                                                                           >  2:3136635/1‑150 (MQ=255)
               ccccAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCt                                                                                                                                      <  1:3115328/150‑1 (MQ=255)
                cccAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGACAGGATAACCAATCtt                                                                                                                                     <  1:3317789/150‑1 (MQ=255)
                    aaTTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACACTAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCAt                                                                                                                                 >  1:463973/1‑150 (MQ=255)
                    aaTTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCAt                                                                                                                                 >  1:2049945/1‑150 (MQ=255)
                     aTTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATa                                                                                                                                >  2:3714976/1‑150 (MQ=255)
                     aTTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATa                                                                                                                                >  2:3714851/1‑150 (MQ=255)
                      ttATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATAc                                                                                                                               >  1:153582/1‑150 (MQ=255)
                       tatataAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACa                                                                                                                              >  2:2093735/1‑150 (MQ=255)
                        atataAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACaa                                                                                                                             <  1:1975144/150‑1 (MQ=255)
                          ataAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATg                                                                                                                           >  2:2770707/1‑150 (MQ=255)
                          ataAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGACAGGATAACCAATCTTTCATACAATg                                                                                                                           <  1:1618071/150‑1 (MQ=255)
                          ataAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGACAGGATAACCAATCTTTCATACAATg                                                                                                                           >  1:3294877/1‑150 (MQ=255)
                              aGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCACAAGCGGAAATTGCTTCGACAATAATTCAATGTTGAGAGGCCAGGATAACCAATCTTTCATACAATGACat                                                                                                                       >  2:3415376/1‑150 (MQ=255)
                                aTGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACatat                                                                                                                     >  1:834007/1‑150 (MQ=255)
                                aTGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACatat                                                                                                                     >  2:3355610/1‑150 (MQ=255)
                                aTGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGACAGGATAACCAATCTTTCATACAATGACatat                                                                                                                     >  1:3974158/1‑150 (MQ=255)
                                   gACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTaa                                                                                                                  >  2:3533526/1‑150 (MQ=255)
                                   gACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTaa                                                                                                                  <  1:3538899/150‑1 (MQ=255)
                                   gACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTaa                                                                                                                  <  1:3784427/150‑1 (MQ=255)
                                    aCAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTaaa                                                                                                                 >  1:846252/1‑150 (MQ=255)
                                    aCAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTaaa                                                                                                                 <  1:4044701/150‑1 (MQ=255)
                                    aCAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGATTCGACAATAATTAAATTTTTAGAGGACAGGATAACCAATCTTTCATACAATGACATATTaaa                                                                                                                 >  2:884342/1‑150 (MQ=255)
                                      aaTATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAat                                                                                                               >  1:2982387/1‑150 (MQ=255)
                                      aaTATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAat                                                                                                               >  2:4059243/1‑150 (MQ=255)
                                        tatCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAatat                                                                                                             <  2:1788730/150‑1 (MQ=255)
                                           cTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCACAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAg                                                                                                          >  2:1621725/1‑150 (MQ=255)
                                            ttGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGc                                                                                                         <  1:3058753/150‑1 (MQ=255)
                                            ttGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGc                                                                                                         <  1:913578/150‑1 (MQ=255)
                                            ttGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGACAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGc                                                                                                         >  1:284910/1‑150 (MQ=255)
                                              gACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATAGCTTCGACAATAATTAAATTTTTAGAGGACAGGATAACCAATCTTTCATACAATGACATATTAAAATATCATCaa                                                                                                       >  1:1680952/1‑150 (MQ=255)
                                                caaacaaaTCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGACAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGa                                                                                                     >  2:1063773/1‑150 (MQ=255)
                                                 aaacaaaTCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGaa                                                                                                    >  2:2004362/1‑150 (MQ=255)
                                                 aaacaaaTCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGaa                                                                                                    >  1:169514/1‑150 (MQ=255)
                                                  aacaaaTCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAAt                                                                                                   <  1:3525175/150‑1 (MQ=255)
                                                  aacaaaTCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAAt                                                                                                   >  1:2114410/1‑150 (MQ=255)
                                                    caaaTCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTc                                                                                                 >  1:773633/1‑150 (MQ=255)
                                                      aaTCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCa                                                                                               <  2:3303850/150‑1 (MQ=255)
                                                       aTCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCaa                                                                                              <  2:557622/150‑1 (MQ=255)
                                                            aGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACa                                                                                         <  2:3829604/150‑1 (MQ=255)
                                                            aGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACa                                                                                         <  1:494297/150‑1 (MQ=255)
                                                                ttATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGt                                                                                     >  1:1040837/1‑150 (MQ=255)
                                                                ttATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGt                                                                                     >  1:605717/1‑150 (MQ=255)
                                                                ttATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGt                                                                                     >  1:1364071/1‑150 (MQ=255)
                                                                     ttCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATaa                                                                                >  2:2342821/1‑150 (MQ=255)
                                                                      tCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAg                                                                               <  1:1016672/150‑1 (MQ=255)
                                                                      tCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAg                                                                               <  1:1063309/150‑1 (MQ=255)
                                                                        aaCATTATTAACCAATAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCa                                                                             <  2:1666334/150‑1 (MQ=255)
                                                                         aCATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCaa                                                                            <  2:1893325/150‑1 (MQ=255)
                                                                          cATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAg                                                                           >  2:1246300/1‑150 (MQ=255)
                                                                             tattaACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTa                                                                        <  1:1726470/150‑1 (MQ=255)
                                                                             tattaACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTa                                                                        >  1:1092822/1‑150 (MQ=255)
                                                                             tattaACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGACAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTa                                                                        <  1:3934249/150‑1 (MQ=255)
                                                                             tattaAACATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTACATACAATGACATATTAAAATAACAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTa                                                                        >  1:3940219/1‑150 (MQ=255)
                                                                              attaACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTaa                                                                       >  1:2536742/1‑150 (MQ=255)
                                                                                 aaCCATTAATGACAGCCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGc                                                                    >  2:3885764/1‑150 (MQ=255)
                                                                                  aCCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGcc                                                                   <  2:3544290/150‑1 (MQ=255)
                                                                                  aCCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGcc                                                                   >  1:2312341/1‑150 (MQ=255)
                                                                                     aTTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCttt                                                                >  1:4175518/1‑150 (MQ=255)
                                                                                      ttAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTa                                                               <  1:1775893/150‑1 (MQ=255)
                                                                                          tGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGac                                                           >  1:465872/1‑150 (MQ=255)
                                                                                                  ttttACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATNAGCAAGTTAAAGCCTTTACGACACTCTccc                                                   <  1:3854192/150‑1 (MQ=255)
                                                                                                  ttttACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTccc                                                   <  1:1756776/150‑1 (MQ=255)
                                                                                                      aCGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATa                                               <  2:1336166/150‑1 (MQ=255)
                                                                                                       cGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATAc                                              >  2:2294881/1‑150 (MQ=255)
                                                                                                          gCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAAt                                           >  2:712253/1‑150 (MQ=255)
                                                                                                            aaaaGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATaa                                         >  2:2425834/1‑150 (MQ=255)
                                                                                                              aaGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATaaaa                                       >  2:1197046/1‑150 (MQ=255)
                                                                                                                  ggAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCa                                   <  1:2861998/150‑1 (MQ=255)
                                                                                                                   gAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCACGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCaa                                  >  2:3849152/1‑150 (MQ=255)
                                                                                                                    aaaTTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCaaa                                 <  1:2126806/150‑1 (MQ=255)
                                                                                                                     aaTTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAAc                                <  1:2255831/150‑1 (MQ=255)
                                                                                                                      aTTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACa                               >  2:3861827/1‑150 (MQ=255)
                                                                                                                      aTTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACa                               >  2:861868/1‑150 (MQ=255)
                                                                                                                      aTTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACa                               >  2:935297/1‑150 (MQ=255)
                                                                                                                      aTTGCTTCGACAATAATTAAATTTTTAGAGGACAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACa                               >  2:393773/1‑150 (MQ=255)
                                                                                                                        tGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATg                             >  1:1893428/1‑150 (MQ=255)
                                                                                                                        tGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATg                             >  2:643415/1‑150 (MQ=255)
                                                                                                                            tCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCa                         <  1:2768721/150‑1 (MQ=255)
                                                                                                                             cGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAg                        >  1:3888848/1‑150 (MQ=255)
                                                                                                                              gACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGa                       >  2:3550808/1‑150 (MQ=255)
                                                                                                                                  ataatTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATg                   >  2:4003898/1‑150 (MQ=255)
                                                                                                                                    aatTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTc                 >  2:2144887/1‑150 (MQ=255)
                                                                                                                                       tAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGa              >  2:2998616/1‑150 (MQ=255)
                                                                                                                                        aaaTTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGaa             <  1:693444/150‑1 (MQ=255)
                                                                                                                                          aTTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAata           >  1:1761479/1‑150 (MQ=255)
                                                                                                                                          aTTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAata           <  1:1076334/150‑1 (MQ=255)
                                                                                                                                          aTTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAata           <  2:661446/150‑1 (MQ=255)
                                                                                                                                               tAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAAt      >  2:2708293/1‑150 (MQ=255)
                                                                                                                                               tAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAAt      <  2:322713/150‑1 (MQ=255)
                                                                                                                                                agagGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAAtt     >  1:2700566/1‑150 (MQ=255)
                                                                                                                                                agagGACAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAAtt     >  2:463751/1‑150 (MQ=255)
                                                                                                                                                 gagGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATCAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTc    >  2:1132652/1‑150 (MQ=255)
                                                                                                                                                 gagGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTc    <  2:137739/150‑1 (MQ=255)
                                                                                                                                                  agGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCg   <  1:3877606/150‑1 (MQ=255)
                                                                                                                                                   ggCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGa  >  2:1931994/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGGAAATTGCTTCGACAATAATTAAATTTTTAGAGGCCAGGATAACCAATCTTTCATACAATGACATATTAAAATATCAGCAAGAATTCCAAAGACAATGTTATAAGCAAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGA  >  CP009273/3646614‑3646910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑