BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,777,313	A→T	5.3%	V59E (GTA→GAA)	secB ←	protein export chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,777,313	0	A	T	5.3%	965.0 / 32.4	281	V59E (GTA→GAA)	secB	protein export chaperone
Reads supporting (aligned to +/- strand): ref base A (130/136); new base T (7/8); total (137/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 285 total aligned reads displayed.

CGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGTC  >  CP009273/3777165‑3777462

cGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTAc                                                                                                                                                      >  1:795245/1‑150 (MQ=255)

cGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTAc                                                                                                                                                      <  2:3903024/150‑1 (MQ=255)

  ggAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCa                                                                                                                                                    <  2:2594711/150‑1 (MQ=255)

       aGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCg                                                                                                                                               >  1:4011092/1‑150 (MQ=255)

         aaTGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTTCCACTTCGta                                                                                                                                             >  1:3957191/1‑150 (MQ=255)

           tGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGtata                                                                                                                                           >  1:1022877/1‑150 (MQ=255)

            gTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATAc                                                                                                                                          <  1:2145548/150‑1 (MQ=255)

                gggCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCa                                                                                                                                      >  1:1836476/1‑150 (MQ=255)

                   cAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCt                                                                                                                                   <  1:2612030/150‑1 (MQ=255)

                    aGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTg                                                                                                                                  <  2:509505/150‑1 (MQ=255)

                    aGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTg                                                                                                                                  >  2:1682101/1‑150 (MQ=255)

                    aGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTg                                                                                                                                  <  1:3885976/150‑1 (MQ=255)

                         gCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGt                                                                                                                             >  2:3023860/1‑150 (MQ=255)

                           tCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGACCGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTCACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTg                                                                                                                           >  2:1187613/1‑150 (MQ=255)

                           tCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTg                                                                                                                           <  2:2892198/150‑1 (MQ=255)

                           tCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTg                                                                                                                           >  2:187356/1‑150 (MQ=255)

                            cccAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTgg                                                                                                                          <  2:3209789/150‑1 (MQ=255)

                             ccAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTggg                                                                                                                         >  1:420165/1‑150 (MQ=255)

                                ggCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAg                                                                                                                      <  1:2690788/150‑1 (MQ=255)

                                  cAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGAt                                                                                                                    <  2:2578929/150‑1 (MQ=255)

                                   aaTGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTTCCACTTCGTATACGTCATCTGCCAGTTGGGAAGATg                                                                                                                   <  2:1030088/150‑1 (MQ=255)

                                       cgcCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGt                                                                                                               >  2:2628490/1‑150 (MQ=255)

                                         ccATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTAt                                                                                                             >  2:3523644/1‑150 (MQ=255)

                                         ccATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTAt                                                                                                             <  1:1617019/150‑1 (MQ=255)

                                          cATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATc                                                                                                            >  2:1367784/1‑150 (MQ=255)

                                           aTCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTTCCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATcc                                                                                                           >  2:2384463/1‑150 (MQ=255)

                                           aTCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATcc                                                                                                           >  2:2770051/1‑150 (MQ=255)

                                             cTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAg                                                                                                         >  2:1322581/1‑150 (MQ=255)

                                              tGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGa                                                                                                        >  1:4053519/1‑150 (MQ=255)

                                               gggTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGAt                                                                                                       <  1:2791818/150‑1 (MQ=255)

                                               gggTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTGACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGAt                                                                                                       <  1:612944/150‑1 (MQ=255)

                                                ggTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATc                                                                                                      <  1:443040/150‑1 (MQ=255)

                                                ggTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATc                                                                                                      <  2:1336167/150‑1 (MQ=255)

                                                 gTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCa                                                                                                     <  1:847842/150‑1 (MQ=255)

                                                      ttCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGttt                                                                                                <  1:1269772/150‑1 (MQ=255)

                                                           tACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACtt                                                                                           >  1:334593/1‑150 (MQ=255)

                                                           tACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACtt                                                                                           <  1:1919327/150‑1 (MQ=255)

                                                            aCCCGCGATGGAGAAAATACCGCCCTGCTGATCTTCTCACAGGATCGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTATCACGCAGTACCACTTCGTATTCGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATAAAGTTTAACTTc                                                                                          <  1:977262/150‑1 (MQ=255)

                                                             cccGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCt                                                                                         <  1:250909/150‑1 (MQ=255)

                                                                 cgATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGtt                                                                                     >  2:952778/1‑150 (MQ=255)

                                                                  gATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTg                                                                                    <  2:130000/150‑1 (MQ=255)

                                                                  gATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTg                                                                                    <  2:1808315/150‑1 (MQ=255)

                                                                   aTGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGc                                                                                   >  1:3784193/1‑150 (MQ=255)

                                                                    tGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGcc                                                                                  >  2:1164567/1‑150 (MQ=255)

                                                                    tGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGcc                                                                                  <  1:1817170/150‑1 (MQ=255)

                                                                          aaaTACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCtt                                                                            >  1:517608/1‑150 (MQ=255)

                                                                                cGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGa                                                                      >  2:3602323/1‑150 (MQ=255)

                                                                                cGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGa                                                                      >  1:720420/1‑150 (MQ=255)

                                                                                  cccTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGaaa                                                                    >  2:3090481/1‑150 (MQ=255)

                                                                                     tgctgAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACg                                                                 >  1:3063154/1‑150 (MQ=255)

                                                                                      gctgAACGTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGt                                                                <  2:1017468/150‑1 (MQ=255)

                                                                                          aaCTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTgcgg                                                            <  1:1544405/150‑1 (MQ=255)

                                                                                              tCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGcgcg                                                        >  1:3564861/1‑150 (MQ=255)

                                                                                              tCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGcgcg                                                        >  2:370003/1‑150 (MQ=255)

                                                                                               cacacaGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGt                                                       <  1:3231184/150‑1 (MQ=255)

                                                                                                acacaGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGcgcggt                                                      >  2:356848/1‑148 (MQ=255)

                                                                                                acacaGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGtt                                                      >  1:2492188/1‑150 (MQ=255)

                                                                                                    aGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGgc                                                  >  1:1690235/1‑150 (MQ=255)

                                                                                                    aGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGgc                                                  >  1:919545/1‑150 (MQ=255)

                                                                                                      gAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGgcgc                                                <  1:4129202/150‑1 (MQ=255)

                                                                                                      gAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGgcgc                                                >  2:2509594/1‑150 (MQ=255)

                                                                                                         cgcgGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTc                                             <  1:1127118/150‑1 (MQ=255)

                                                                                                          gcgGTTTCTTCGCCCAAAGATGCCGTTACGGTAACAAGCATTACCACTTCTTATACGTCATCTGCCATTTTGGAAGATTCCGTATCCAGATCAAGTTTAACTTCTTGTTGCCAATCTTTATGGATAACGTGCGGCGCGTTCGGCGCTTCg                                            <  1:142660/150‑1 (MQ=255)

                                                                                                          gcgGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTTCCACTTCGTATCCGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTNCg                                            <  1:882990/150‑1 (MQ=255)

                                                                                                           cgGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGa                                           <  1:3102597/150‑1 (MQ=255)

                                                                                                             gTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGaaa                                         <  1:2966932/150‑1 (MQ=255)

                                                                                                               ttcttcGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAaga                                       <  2:1881475/150‑1 (MQ=255)

                                                                                                                tcttcGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAagag                                      <  2:2091390/150‑1 (MQ=255)

                                                                                                                tcttcGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAagag                                      <  1:2411635/150‑1 (MQ=255)

                                                                                                                  ttcGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGat                                    <  1:1947609/150‑1 (MQ=255)

                                                                                                                    cGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGatat                                  >  2:595635/1‑150 (MQ=255)

                                                                                                                    cGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGatat                                  >  2:2450844/1‑150 (MQ=255)

                                                                                                                    cGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGatat                                  >  2:904176/1‑150 (MQ=255)

                                                                                                                    cGCCCAAAGAGGCCGTTACGGTAACACGCAGAACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGatat                                  >  1:2654547/1‑150 (MQ=255)

                                                                                                                     gCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATc                                 >  2:3714765/1‑150 (MQ=255)

                                                                                                                     gCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATc                                 >  2:4161116/1‑150 (MQ=255)

                                                                                                                     gCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATc                                 >  2:3852012/1‑150 (MQ=255)

                                                                                                                     gCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCAGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATc                                 >  2:2961973/1‑150 (MQ=255)

                                                                                                                       ccAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCt                               >  1:301209/1‑150 (MQ=255)

                                                                                                                         aaaGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTg                             >  2:2796178/1‑150 (MQ=255)

                                                                                                                             agGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGtat                         >  1:1231442/1‑150 (MQ=255)

                                                                                                                              ggCCGGTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTACCTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGtata                        <  1:1408887/150‑1 (MQ=255)

                                                                                                                                ccGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATaaa                      >  2:3017528/1‑150 (MQ=255)

                                                                                                                                ccGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATaaa                      <  1:3380698/150‑1 (MQ=255)

                                                                                                                                 cGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAAt                     >  1:2921102/1‑150 (MQ=255)

                                                                                                                                    tACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACg                  >  1:14615/1‑150 (MQ=255)

                                                                                                                                     aCGGTAACACGCAGTTCCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGt                 <  2:3996083/150‑1 (MQ=255)

                                                                                                                                       ggTAACACGCAGTTCCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTg               >  1:1360282/1‑150 (MQ=255)

                                                                                                                                       ggTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTg               <  2:667288/150‑1 (MQ=255)

                                                                                                                                           acacGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATc           <  2:2140804/150‑1 (MQ=255)

                                                                                                                                           acacGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATc           <  1:3509715/150‑1 (MQ=255)

                                                                                                                                           acacGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATc           <  1:2852029/150‑1 (MQ=255)

                                                                                                                                            cacGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCt          >  1:226016/1‑150 (MQ=255)

                                                                                                                                            cacGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCt          >  1:2307386/1‑150 (MQ=255)

                                                                                                                                            cacGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCt          <  1:3624812/150‑1 (MQ=255)

                                                                                                                                                cAGTACCACTTCGTATACGTCATCTGCCAGTTTGTAAGATTCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGaa      <  1:3703551/150‑1 (MQ=255)

                                                                                                                                                 aGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGaaa     >  2:798819/1‑150 (MQ=255)

                                                                                                                                                 aGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGaaa     <  1:3392388/150‑1 (MQ=255)

                                                                                                                                                   tACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGt   <  2:2295490/150‑1 (MQ=255)

                                                                                                                                                    aCCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGTc  <  1:105004/150‑1 (MQ=255)

CGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGTC  >  CP009273/3777165‑3777462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`