Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,819,936 C→A 44.3% T456K (ACG→AAG)  recG → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,819,9360CA44.3% 227.2 / 301.5 241T456K (ACG→AAG) recGATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base C (66/68);  new base A (60/47);  total (126/115)
Fisher's exact test for biased strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

GCGTCTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAA  >  CP009273/3819787‑3820084
                                                                                                                                                     |                                                                                                                                                    
gCGTCTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAc                                                                                                                                                      <  1:1725915/150‑1 (MQ=255)
  gTCTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGc                                                                                                                                                    >  2:434325/1‑150 (MQ=255)
    cTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCa                                                                                                                                                  <  2:825948/150‑1 (MQ=255)
       gCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTg                                                                                                                                               >  1:2825369/1‑150 (MQ=255)
             tgGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACg                                                                                                                                         <  2:3345243/150‑1 (MQ=255)
              gggAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACgg                                                                                                                                        <  2:376440/150‑1 (MQ=255)
               ggAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGt                                                                                                                                       <  2:1451793/150‑1 (MQ=255)
               ggAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGt                                                                                                                                       <  2:833583/150‑1 (MQ=255)
                gagtAAGGCCTGGAGCAGGGATTCCATCCGCTTCAGTTGATCTTGATGGCCACGCCGATCAACGGCTCGATGTGTATGACTGGGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGTTTACTGTc                                                                                                                                      <  2:1337349/150‑1 (MQ=255)
                gagaAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTc                                                                                                                                      <  2:618988/150‑1 (MQ=255)
                 agaAAGGCCAGCAGCAGGGCTTCCATCCGCATAAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCg                                                                                                                                     >  2:2466978/1‑150 (MQ=255)
                  gaAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGc                                                                                                                                    >  1:2971526/1‑150 (MQ=255)
                   aaaGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCt                                                                                                                                   >  2:3793696/1‑150 (MQ=255)
                    aaGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTa                                                                                                                                  <  1:2516511/150‑1 (MQ=255)
                    aaGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTa                                                                                                                                  >  1:3513922/1‑150 (MQ=255)
                      ggCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTAtt                                                                                                                                >  1:3109922/1‑150 (MQ=255)
                       gCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTc                                                                                                                               <  2:52811/150‑1 (MQ=255)
                       gCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTc                                                                                                                               <  2:3266789/150‑1 (MQ=255)
                        ccAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTcc                                                                                                                              <  1:392551/150‑1 (MQ=255)
                        ccAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTcc                                                                                                                              >  1:2500515/1‑150 (MQ=255)
                         cagcagcagGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCt                                                                                                                             <  1:154995/150‑1 (MQ=255)
                           gcagcagGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGa                                                                                                                           <  2:3868004/150‑1 (MQ=255)
                            cagcagGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGAt                                                                                                                          <  2:3630356/150‑1 (MQ=255)
                                    cTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACccgccg                                                                                                                  <  2:2372365/150‑1 (MQ=255)
                                    cTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACccgccg                                                                                                                  <  2:3929248/150‑1 (MQ=255)
                                     ttCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGt                                                                                                                 >  2:2643902/1‑150 (MQ=255)
                                         aTCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCg                                                                                                             >  1:3639623/1‑150 (MQ=255)
                                          tCCGCATCAGTTGATCATGACAGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGa                                                                                                            <  1:3458299/150‑1 (MQ=255)
                                              cATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGAcatc                                                                                                        >  1:2342541/1‑150 (MQ=255)
                                              cATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGAcatc                                                                                                        <  2:3629283/150‑1 (MQ=255)
                                                  aGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTg                                                                                                    <  2:3795467/150‑1 (MQ=255)
                                                   gTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGa                                                                                                   <  1:306281/150‑1 (MQ=255)
                                                    ttGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGAc                                                                                                  <  2:2565740/150‑1 (MQ=255)
                                                      gATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACcg                                                                                                <  2:3450650/150‑1 (MQ=255)
                                                           tGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGc                                                                                           >  2:3718499/1‑150 (MQ=255)
                                                           tGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGc                                                                                           >  1:2446468/1‑150 (MQ=255)
                                                              ccGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGcacc                                                                                        >  2:2260874/1‑150 (MQ=255)
                                                               cGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGcacca                                                                                       <  2:3103767/150‑1 (MQ=255)
                                                                 ccACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACg                                                                                     <  2:1938833/150‑1 (MQ=255)
                                                                  cACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGc                                                                                    >  2:860475/1‑150 (MQ=255)
                                                                  cACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGc                                                                                    >  2:860390/1‑150 (MQ=255)
                                                                    cGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCt                                                                                  >  2:2273960/1‑150 (MQ=255)
                                                                    cGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCt                                                                                  <  1:924074/150‑1 (MQ=255)
                                                                    cGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCt                                                                                  >  2:2380052/1‑150 (MQ=255)
                                                                    cGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCt                                                                                  <  2:697368/150‑1 (MQ=255)
                                                                      ccGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGc                                                                                >  2:3017588/1‑150 (MQ=255)
                                                                       cGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCa                                                                               >  2:2524768/1‑150 (MQ=255)
                                                                        gATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCAt                                                                              >  1:736390/1‑150 (MQ=255)
                                                                        gATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCCGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCAt                                                                              >  2:1584579/1‑150 (MQ=255)
                                                                         aTCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATa                                                                             >  2:1419644/1‑150 (MQ=255)
                                                                          tCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATaa                                                                            <  1:3246035/150‑1 (MQ=255)
                                                                            ccccGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACt                                                                          <  1:1818142/150‑1 (MQ=255)
                                                                             cccGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTg                                                                         <  1:1863994/150‑1 (MQ=255)
                                                                                 cACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAgg                                                                     >  1:3404337/1‑150 (MQ=255)
                                                                                   cGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGgtc                                                                   <  1:1793762/150‑1 (MQ=255)
                                                                                    gCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGgtcg                                                                  <  2:3525807/150‑1 (MQ=255)
                                                                                     cTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGgtcgt                                                                 >  2:510287/1‑150 (MQ=255)
                                                                                      tGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGgtcgtc                                                                >  1:1614955/1‑150 (MQ=255)
                                                                                       ggCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCa                                                               <  2:2964567/150‑1 (MQ=255)
                                                                                       ggCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCa                                                               <  1:3020449/150‑1 (MQ=255)
                                                                                         cAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAgg                                                             >  1:1843870/1‑150 (MQ=255)
                                                                                         cAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAgg                                                             >  2:3573959/1‑150 (MQ=255)
                                                                                            tGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCAt                                                          >  1:643222/1‑150 (MQ=255)
                                                                                            tGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCAt                                                          <  1:554094/150‑1 (MQ=255)
                                                                                            tGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTACTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCAt                                                          >  1:384723/1‑150 (MQ=255)
                                                                                             gACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATa                                                         >  1:2954339/1‑150 (MQ=255)
                                                                                             gACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATa                                                         <  1:2117006/150‑1 (MQ=255)
                                                                                               cTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACt                                                       >  1:1024904/1‑150 (MQ=255)
                                                                                               cTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACt                                                       <  1:1069765/150‑1 (MQ=255)
                                                                                                  cGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTggg                                                    <  2:1059759/150‑1 (MQ=255)
                                                                                                  cGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCGATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCCTAACTGAAGGTCGTCAGGCATACTggg                                                    >  1:1127262/1‑150 (MQ=255)
                                                                                                   gTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGt                                                   >  2:943913/1‑150 (MQ=255)
                                                                                                         cGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTAc                                             <  1:1030525/150‑1 (MQ=255)
                                                                                                           aTCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGt                                           >  2:2871866/1‑150 (MQ=255)
                                                                                                              tcGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttga                                        >  1:3119046/1‑150 (MQ=255)
                                                                                                               cGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATATCTGAAGGTCGTCAGGCATACTGGGTTTGTACGttgat                                       <  1:82057/150‑1 (MQ=255)
                                                                                                                gATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttgatt                                      >  2:1341385/1‑150 (MQ=255)
                                                                                                                gATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttgatt                                      <  1:586359/150‑1 (MQ=255)
                                                                                                                    ccTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAag                                  <  2:4041887/150‑1 (MQ=255)
                                                                                                                    ccTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGGTTGTACGTTGATTGAag                                  >  2:2170137/1‑150 (MQ=255)
                                                                                                                     cTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAaga                                 >  1:1339975/1‑150 (MQ=255)
                                                                                                                             tAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGCAt                         >  1:720756/1‑150 (MQ=255)
                                                                                                                              aGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAAtt                        >  1:2559782/1‑150 (MQ=255)
                                                                                                                              aGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCGGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACAGAAGGTCGTCAGGCATACTGGGGTTGTACGTTTATTTAAGAGTCGGAAtt                        >  1:690056/1‑150 (MQ=255)
                                                                                                                              aGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCCGGCATACTGGGGTTGTACGTTGATTGACGTGTCGGAAtt                        >  1:3748528/1‑150 (MQ=255)
                                                                                                                              aGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAAtt                        <  2:3149946/150‑1 (MQ=255)
                                                                                                                              aGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAAtt                        >  1:2711199/1‑150 (MQ=255)
                                                                                                                               gATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTg                       >  1:1663789/1‑150 (MQ=255)
                                                                                                                                   agatGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTgg                   >  1:3195053/4‑150 (MQ=255)
                                                                                                                                      tGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAg                >  1:4103223/1‑150 (MQ=255)
                                                                                                                                        ccgccAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATTCTTGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAgcg              <  1:1409806/150‑1 (MQ=255)
                                                                                                                                          gccAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCa            <  2:3783063/150‑1 (MQ=255)
                                                                                                                                            cAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAgg          >  2:1826837/1‑150 (MQ=255)
                                                                                                                                              ggCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAggcg        <  1:128179/150‑1 (MQ=255)
                                                                                                                                               gCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAggcgg       <  1:2606414/150‑1 (MQ=255)
                                                                                                                                               gCCGCACGCCAGTGACTACGGTCGCTATTCCAGATACCCGCCGTACCGACAACATTGAACGCGGGCACCCCGCCTGCATAACAGAAAGTCCTCAGGCATACTGGGTTTGTACGTTTATTGAAGGGTCGGAATTGCGGGAAGCGCAggtgg       >  1:1159786/1‑150 (MQ=255)
                                                                                                                                               accGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAggcgg       <  2:805461/149‑1 (MQ=255)
                                                                                                                                                  gCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGcggcgg    <  1:1495229/150‑1 (MQ=255)
                                                                                                                                                   cACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGa   <  1:2069135/150‑1 (MQ=255)
                                                                                                                                                    aCGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGaa  <  1:2019254/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCGTCTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAA  >  CP009273/3819787‑3820084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑