BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,858,274	A→C	35.6%	F454V (TTT→GTT)	yidE ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,858,274	0	A	C	35.6%	156.1 / 305.2	236	F454V (TTT→GTT)	yidE	putative transporter
Reads supporting (aligned to +/- strand): ref base A (92/60); new base C (40/44); total (132/104)
Fisher's exact test for biased strand distribution p-value = 7.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

Only 100 of 245 total aligned reads displayed.

GGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGC  >  CP009273/3858125‑3858423

 gAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACaaaa                                                                                                                                                      >  2:2289928/1‑150 (MQ=255)

 gAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACaaaa                                                                                                                                                      >  1:1437748/1‑150 (MQ=255)

 gAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACaaaa                                                                                                                                                      <  1:566198/150‑1 (MQ=255)

      tGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATcccc                                                                                                                                                 <  1:3768782/150‑1 (MQ=255)

       gCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCcca                                                                                                                                                <  2:3327885/150‑1 (MQ=255)

        ccAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCccac                                                                                                                                               <  1:3169905/150‑1 (MQ=255)

        ccAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCccac                                                                                                                                               >  1:3573476/1‑150 (MQ=255)

         cAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCccacc                                                                                                                                              >  1:1988793/1‑150 (MQ=255)

           gCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAg                                                                                                                                            <  2:3942200/150‑1 (MQ=255)

           gCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAg                                                                                                                                            <  2:2604509/150‑1 (MQ=255)

               cccGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGAttt                                                                                                                                        <  2:3890076/150‑1 (MQ=255)

                ccGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTc                                                                                                                                       >  1:1695218/1‑150 (MQ=255)

                   cacaTGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGa                                                                                                                                    >  2:3388109/1‑150 (MQ=255)

                       tGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCaa                                                                                                                                >  1:2558843/1‑150 (MQ=255)

                           cAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGAc                                                                                                                            <  1:1507330/150‑1 (MQ=255)

                           cAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCACCACCAGATTTCAGACCAACGAc                                                                                                                            >  2:1175201/1‑150 (MQ=255)

                            aGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGAcc                                                                                                                           <  1:1500115/150‑1 (MQ=255)

                            aGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGAcc                                                                                                                           <  1:3099783/150‑1 (MQ=255)

                                aaTTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCgaga                                                                                                                       <  2:3737164/150‑1 (MQ=255)

                                 attatttttGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGagag                                                                                                                      <  1:2778291/145‑1 (MQ=255)

                                 aTTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGagag                                                                                                                      >  1:3319148/1‑150 (MQ=255)

                                 aTTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGagag                                                                                                                      >  1:1681288/1‑150 (MQ=255)

                                     aTTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTCTTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAAc                                                                                                                  >  1:16988/1‑150 (MQ=255)

                                      ttttGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACa                                                                                                                 <  1:460219/150‑1 (MQ=255)

                                      ttttGGCTAACATCCGCGACAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACa                                                                                                                 <  2:1260079/150‑1 (MQ=255)

                                          ggCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCAc                                                                                                             <  1:3378230/150‑1 (MQ=255)

                                          ggCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCAc                                                                                                             <  2:1503191/150‑1 (MQ=255)

                                             tAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGAt                                                                                                          <  1:15878/150‑1 (MQ=255)

                                             tAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGAt                                                                                                          >  2:315860/1‑150 (MQ=255)

                                               aCATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATcc                                                                                                        <  2:2172482/150‑1 (MQ=255)

                                                cATCTGCGCCAGACTGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACTACCGAGAGGAACAGCACGATccc                                                                                                       <  2:1984098/150‑1 (MQ=255)

                                                     gcgcCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCTGCt                                                                                                  <  2:3268066/150‑1 (MQ=255)

                                                      cgcCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTc                                                                                                 >  1:1047206/1‑150 (MQ=255)

                                                       gcCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTcc                                                                                                <  1:3762125/150‑1 (MQ=255)

                                                        ccAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTccc                                                                                               <  2:673474/150‑1 (MQ=255)

                                                           gAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCa                                                                                            >  1:3765600/1‑150 (MQ=255)

                                                            aaTGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAg                                                                                           <  2:2681994/150‑1 (MQ=255)

                                                             aTGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAgc                                                                                          >  1:2045528/1‑150 (MQ=255)

                                                                  aaCAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAg                                                                                     >  2:3084557/1‑150 (MQ=255)

                                                                       tAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTgg                                                                                >  2:3431255/1‑150 (MQ=255)

                                                                       tAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTgg                                                                                >  2:1006377/1‑150 (MQ=255)

                                                                           cAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCt                                                                            >  2:1218784/1‑150 (MQ=255)

                                                                           cAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCt                                                                            <  2:2639288/150‑1 (MQ=255)

                                                                           cAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCt                                                                            >  2:3467394/1‑150 (MQ=255)

                                                                               ggAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTggc                                                                        <  1:2657300/150‑1 (MQ=255)

                                                                               ggAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTggc                                                                        <  2:3806106/150‑1 (MQ=255)

                                                                                 aaCGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTggcgg                                                                      >  2:1320355/1‑150 (MQ=255)

                                                                                   cggcggTTTTCAGGGCACCATAACCAATCCAGCTTTGCGTTTCTCGATTGACCAGGGTATTCACAACTTCCCCACAAGTTTTCAGACCAACGACCGAGAGGAAGAGCACGATCCCCAGCTCCCGCAGCTCTAGGTTGGCGCtttgaggtt                                                                    <  2:1046284/150‑8 (MQ=255)

                                                                                    ggcggTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCAt                                                                   >  2:2122302/1‑150 (MQ=255)

                                                                                          gATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGACCAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCa                                                             >  2:2712071/1‑150 (MQ=255)

                                                                                            tCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGt                                                           >  2:3038435/1‑150 (MQ=255)

                                                                                            tCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGt                                                           <  1:1809481/150‑1 (MQ=255)

                                                                                             cAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTa                                                          >  2:762429/1‑150 (MQ=255)

                                                                                              aGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTAc                                                         >  2:1563393/1‑150 (MQ=255)

                                                                                               gggCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACa                                                        <  1:4176413/150‑1 (MQ=255)

                                                                                                  cACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCt                                                     >  2:3661841/1‑150 (MQ=255)

                                                                                                  cACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCt                                                     <  2:352687/150‑1 (MQ=255)

                                                                                                   aCCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCtt                                                    >  1:2257245/1‑150 (MQ=255)

                                                                                                      aTAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgcc                                                 <  1:658123/150‑1 (MQ=255)

                                                                                                      aTAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgcc                                                 <  1:2793116/150‑1 (MQ=255)

                                                                                                      aTAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgcc                                                 <  1:2793038/150‑1 (MQ=255)

                                                                                                       tAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccg                                                <  1:1070363/150‑1 (MQ=255)

                                                                                                         aCCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccgat                                              >  2:2609431/1‑150 (MQ=255)

                                                                                                         aCCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccgat                                              <  2:2910522/150‑1 (MQ=255)

                                                                                                         aCCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccgat                                              >  2:64794/1‑150 (MQ=255)

                                                                                                         aCCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccgat                                              <  1:3070842/150‑1 (MQ=255)

                                                                                                         aCCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccgat                                              <  1:721063/150‑1 (MQ=255)

                                                                                                            aaTCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccgatact                                           >  1:3436752/1‑150 (MQ=255)

                                                                                                             aTCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccgatactg                                          <  2:1734400/150‑1 (MQ=255)

                                                                                                                cAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccgatactgccg                                       >  1:1289891/1‑150 (MQ=255)

                                                                                                                 aGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAGCCAGTACAGCTtgccgatactgccga                                      >  1:754919/1‑150 (MQ=255)

                                                                                                                 aGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccgatactgccga                                      >  1:3140614/1‑150 (MQ=255)

                                                                                                                  gCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACTAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccgatactgccgat                                     <  2:2841124/150‑1 (MQ=255)

                                                                                                                   cTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccgatactgccgata                                    <  1:1009710/150‑1 (MQ=255)

                                                                                                                   cTTAGCCCTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccgatactgccgata                                    <  1:3099840/150‑1 (MQ=255)

                                                                                                                   cTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccgatactgccgata                                    <  2:2529620/150‑1 (MQ=255)

                                                                                                                    ttAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGAAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTtgccgatactgccgatac                                   <  1:771102/150‑1 (MQ=255)

                                                                                                                       gCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGcc                                <  2:149830/150‑1 (MQ=255)

                                                                                                                         ccTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCg                              >  1:874668/1‑150 (MQ=255)

                                                                                                                         ccTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCg                              >  1:3588797/1‑150 (MQ=255)

                                                                                                                          cTTCGCCATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGa                             >  2:1358105/1‑150 (MQ=255)

                                                                                                                            tCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAgg                           <  1:4023892/150‑1 (MQ=255)

                                                                                                                            tCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAgg                           <  1:1605900/150‑1 (MQ=255)

                                                                                                                                cATTGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCa                       <  2:724366/150‑1 (MQ=255)

                                                                                                                                cATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCa                       >  1:320641/1‑150 (MQ=255)

                                                                                                                                 aTTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCaa                      >  2:3890888/1‑150 (MQ=255)

                                                                                                                                   tGACCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGAGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTCCCGATACTGCCGATACGCCCGAGGATCAACg                    >  1:2065051/1‑150 (MQ=255)

                                                                                                                                   tGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACg                    >  2:3612287/1‑150 (MQ=255)

                                                                                                                                     aCCAGGGTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGcc                  <  2:2789407/150‑1 (MQ=255)

                                                                                                                                      ccAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCa                 >  2:379167/1‑150 (MQ=255)

                                                                                                                                       cAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCAt                >  1:3334395/1‑150 (MQ=255)

                                                                                                                                        aGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATa               >  2:1277931/1‑150 (MQ=255)

                                                                                                                                        aGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATa               >  1:2927460/1‑150 (MQ=255)

                                                                                                                                          ggTATTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAAt             <  2:1882372/150‑1 (MQ=255)

                                                                                                                                          ggTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAAt             <  1:1371630/150‑1 (MQ=255)

                                                                                                                                            tATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCa           <  1:11656/150‑1 (MQ=255)

                                                                                                                                            tATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCa           >  2:512932/1‑150 (MQ=255)

                                                                                                                                             aTTCACAACATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAg          >  1:242282/1‑150 (MQ=255)

                                                                                                                                             aTTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAg          >  1:1770506/1‑150 (MQ=255)

                                                                                                                                                cacaAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCgg       >  1:2143596/1‑150 (MQ=255)

GGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGGAACGGCGGTGATCAGGGCACCATAACCAATCCAGCTTAGCCCTTCGCCATTGACCAGGGTATTCACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACAGCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCGCTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGC  >  CP009273/3858125‑3858423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`