Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,867,062 C→A 35.3% R258L (CGC→CTC)  dgoK ← 2‑oxo‑3‑deoxygalactonate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,867,0620CA35.3% 482.9 / 302.0 300R258L (CGC→CTC) dgoK2‑oxo‑3‑deoxygalactonate kinase
Reads supporting (aligned to +/- strand):  ref base C (101/93);  new base A (60/46);  total (161/139)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

Only 100 of 314 total aligned reads displayed.

CGTCGGGCGTAATACCGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAGTGTTCCCAGCACATGCGAGGCG  >  CP009273/3866915‑3867211
                                                                                                                                                   |                                                                                                                                                     
 gtcgGGCGTAATACCGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAgcgcg                                                                                                                                                    <  1:3880617/150‑1 (MQ=255)
 gtcgGGCGTAATACCGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAgagcg                                                                                                                                                    <  1:172359/150‑1 (MQ=255)
  tcgGGCGTAATACCGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAgagcgc                                                                                                                                                   <  2:2171474/150‑1 (MQ=255)
     ggCGTAATACCGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGt                                                                                                                                                <  2:746597/150‑1 (MQ=255)
     ggCGTAATACCGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGt                                                                                                                                                <  2:348794/150‑1 (MQ=255)
       cGTAATACCGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCa                                                                                                                                              >  2:2847781/1‑150 (MQ=255)
          aaTACCGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCg                                                                                                                                           >  2:3748539/1‑150 (MQ=255)
          aaTACCGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCg                                                                                                                                           >  2:1650737/1‑150 (MQ=255)
          aaTACCGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCg                                                                                                                                           >  2:2323956/1‑150 (MQ=255)
          aaTACCGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCg                                                                                                                                           >  2:2787523/1‑150 (MQ=255)
            tACCGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGAt                                                                                                                                         <  1:1604800/150‑1 (MQ=255)
            tACCGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGAt                                                                                                                                         <  1:1971683/150‑1 (MQ=255)
              ccGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGt                                                                                                                                       >  2:3058731/1‑150 (MQ=255)
                  gcAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCg                                                                                                                                   <  2:3948844/150‑1 (MQ=255)
                   cAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCgg                                                                                                                                  >  2:3002657/1‑150 (MQ=255)
                   cAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCgg                                                                                                                                  >  2:2090103/1‑150 (MQ=255)
                       aTGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGAc                                                                                                                              >  1:1337182/1‑150 (MQ=255)
                         ggCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACaa                                                                                                                            >  2:887307/1‑150 (MQ=255)
                           cGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAgg                                                                                                                          >  1:3278710/1‑150 (MQ=255)
                              tCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTg                                                                                                                       >  2:458501/1‑150 (MQ=255)
                               cAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGa                                                                                                                      >  2:3877835/1‑150 (MQ=255)
                               cAGCGGGAGGTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGa                                                                                                                      >  1:3925382/1‑150 (MQ=255)
                                aGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATCCCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGAt                                                                                                                     <  1:3490335/150‑1 (MQ=255)
                                aGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGAt                                                                                                                     >  2:1171402/1‑150 (MQ=255)
                                aGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGAt                                                                                                                     >  1:1478551/1‑150 (MQ=255)
                                   gggAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGc                                                                                                                  >  2:2645035/1‑150 (MQ=255)
                                     gAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCgt                                                                                                                <  1:3418445/150‑1 (MQ=255)
                                       gtttagtttGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCgtgt                                                                                                              <  1:2749170/150‑1 (MQ=255)
                                       gtttagtttGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCgtgt                                                                                                              <  2:1079927/150‑1 (MQ=255)
                                        tttagtttGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGtt                                                                                                             <  1:2854638/150‑1 (MQ=255)
                                           agtttGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGAt                                                                                                          >  1:2679919/1‑150 (MQ=255)
                                           agtttGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGAt                                                                                                          >  1:365502/1‑150 (MQ=255)
                                           agtttGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGAt                                                                                                          <  2:3550506/150‑1 (MQ=255)
                                            gtttGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATg                                                                                                         >  1:1795896/1‑150 (MQ=255)
                                              ttGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATggg                                                                                                       <  1:3446207/150‑1 (MQ=255)
                                              ttGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATggg                                                                                                       >  1:1889218/1‑150 (MQ=255)
                                                 ccACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCca                                                                                                    >  2:2141007/1‑150 (MQ=255)
                                                 ccACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCca                                                                                                    >  1:57309/1‑150 (MQ=255)
                                                 ccACTGCATGAGCGAGGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCca                                                                                                    <  1:1128731/150‑1 (MQ=255)
                                                     tGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATa                                                                                                <  1:878095/150‑1 (MQ=255)
                                                        aTGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTc                                                                                             >  2:4121300/1‑150 (MQ=255)
                                                             cGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCa                                                                                        <  2:2401041/150‑1 (MQ=255)
                                                              gATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCAt                                                                                       >  1:295596/1‑150 (MQ=255)
                                                              gATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCAt                                                                                       <  1:1776128/150‑1 (MQ=255)
                                                              gATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCAt                                                                                       >  2:572454/1‑150 (MQ=255)
                                                               aTGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATa                                                                                      >  1:4153507/1‑150 (MQ=255)
                                                                tGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATAc                                                                                     <  2:1404333/150‑1 (MQ=255)
                                                                tGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATAc                                                                                     <  1:987352/150‑1 (MQ=255)
                                                                tGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATAc                                                                                     <  1:3047538/150‑1 (MQ=255)
                                                                tGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATAc                                                                                     <  2:227901/150‑1 (MQ=255)
                                                                 gCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACt                                                                                    <  2:834840/150‑1 (MQ=255)
                                                                  cTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTg                                                                                   >  2:2444879/1‑150 (MQ=255)
                                                                  cTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTg                                                                                   >  2:1054942/1‑150 (MQ=255)
                                                                   tCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTgg                                                                                  >  1:838942/1‑150 (MQ=255)
                                                                      ttATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGa                                                                               >  1:1369643/1‑150 (MQ=255)
                                                                      ttATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGa                                                                               >  1:2379410/1‑150 (MQ=255)
                                                                       tataCCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGAc                                                                              >  2:1919419/1‑150 (MQ=255)
                                                                           ccAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACctct                                                                          <  2:707204/150‑1 (MQ=255)
                                                                            cAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTg                                                                         <  1:3110069/150‑1 (MQ=255)
                                                                                  gAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCaa                                                                   >  1:1021488/1‑150 (MQ=255)
                                                                                  gAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCaa                                                                   >  2:3325595/1‑150 (MQ=255)
                                                                                   aaaTGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAAt                                                                  <  2:3188685/150‑1 (MQ=255)
                                                                                     aTGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATca                                                                >  2:1589785/1‑150 (MQ=255)
                                                                                     aTGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATca                                                                >  2:2029631/1‑150 (MQ=255)
                                                                                       gCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATcaac                                                              >  2:254444/1‑150 (MQ=255)
                                                                                         cGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATcaacaa                                                            <  1:2041591/150‑1 (MQ=255)
                                                                                               gcccgccACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCaga                                                      >  2:2918701/1‑150 (MQ=255)
                                                                                               gcccgccACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCaga                                                      <  1:1534215/150‑1 (MQ=255)
                                                                                                  cgccACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCagagag                                                   >  1:2353743/1‑150 (MQ=255)
                                                                                                     cACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGaaa                                                <  2:1551102/150‑1 (MQ=255)
                                                                                                        cgccgTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTc                                             <  1:1425242/150‑1 (MQ=255)
                                                                                                         gccgTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCg                                            <  1:4104114/150‑1 (MQ=255)
                                                                                                         gccgTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCg                                            <  1:3312754/150‑1 (MQ=255)
                                                                                                          ccgTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGc                                           <  2:3254711/150‑1 (MQ=255)
                                                                                                            gTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTg                                         <  2:1983875/150‑1 (MQ=255)
                                                                                                             tCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGa                                        <  1:16508/150‑1 (MQ=255)
                                                                                                                cGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCt                                     >  1:1653334/1‑150 (MQ=255)
                                                                                                                cGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCt                                     >  1:3707387/1‑150 (MQ=255)
                                                                                                                  tCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGt                                   <  1:2323956/150‑1 (MQ=255)
                                                                                                                   cGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGtt                                  >  2:3139109/1‑150 (MQ=255)
                                                                                                                    gCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTc                                 <  2:3863025/150‑1 (MQ=255)
                                                                                                                          cATCGCCTGAAAGGCTTGTTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCgggg                           <  1:1691726/150‑1 (MQ=255)
                                                                                                                             cGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAg                        <  2:2889527/150‑1 (MQ=255)
                                                                                                                              gCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAgt                       <  1:3596324/150‑1 (MQ=255)
                                                                                                                              gCCTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAgt                       <  1:1991683/150‑1 (MQ=255)
                                                                                                                               ccTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAgtg                      <  1:1958801/150‑1 (MQ=255)
                                                                                                                                cTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAgtgt                     >  1:3249653/1‑150 (MQ=255)
                                                                                                                                cTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAgtgt                     <  1:491749/150‑1 (MQ=255)
                                                                                                                                cTGAAAGGCTTGCTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAgtgt                     >  1:725946/1‑150 (MQ=255)
                                                                                                                                 tGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAGTGtt                    >  1:431971/1‑150 (MQ=255)
                                                                                                                                 tGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAGTGtt                    >  1:3357200/1‑150 (MQ=255)
                                                                                                                                   aaaGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGTGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAGTGTTcc                  >  2:2939984/1‑150 (MQ=255)
                                                                                                                                    aaGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAGTGTTccc                 >  1:294799/1‑150 (MQ=255)
                                                                                                                                    aaGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAGTGTTccc                 <  2:2203336/150‑1 (MQ=255)
                                                                                                                                    aaGGCTTGCTGGTAGCGCGCGGTCAGCGATGGTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAGTGTTccc                 <  1:2586014/150‑1 (MQ=255)
                                                                                                                                            cTGGTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAGTGTTCCCAGCACATg         <  1:2644702/150‑1 (MQ=255)
                                                                                                                                              ggTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAGTGTTCCCAGCACATGCg       <  1:2847781/150‑1 (MQ=255)
                                                                                                                                               gTAGAGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAGTGTTCCCAGCACATGCGa      <  2:1808461/150‑1 (MQ=255)
                                                                                                                                                 aGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAGTGTTCCCAGCACATGCGAgg    <  1:3748539/150‑1 (MQ=255)
                                                                                                                                                   cgcgcgGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCCGAGAGAAATTCGCTGACCTGTTCGCGGGGAAGTGTTCCCAGCACATGCGAGgcg  <  2:651120/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGTCGGGCGTAATACCGCGCAAAATGGCGATCAGCGGGAGTTTAGTTTGCCACTGCATGAGCGATGCTCCTTATACCAGCCTGAAATGCCGTGTCGCCCGCCACCGCCGTCACGTCGCAACCCATCGCCTGAAAGGCTTGCTGGTAGCGCGCGGTCAGCGATGTTCCGGCGACAAGGGTGATGGCGTGTTGATGGGCCACATAGTCGCGCATACTGGCGACCTCTGCGCCAATCAACAAACCAGAGAGAAATTCGCTGACCTGTTCGCGGGGAAGTGTTCCCAGCACATGCGAGGCG  >  CP009273/3866915‑3867211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑