Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,910,894 C→A 100% M244I (ATG→ATT)  atpG ← F1 sector of membrane‑bound ATP synthase, gamma subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,910,8940CA99.1% 818.1 / ‑1.5 232M244I (ATG→ATT) atpGF1 sector of membrane‑bound ATP synthase, gamma subunit
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (141/89);  total (141/91)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

ACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCAT  >  CP009273/3910748‑3911042
                                                                                                                                                  |                                                                                                                                                    
aCGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATa                                                                                                                                                   >  2:2403552/1‑150 (MQ=255)
 cGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATAc                                                                                                                                                  >  2:1118616/1‑150 (MQ=255)
 cGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATAc                                                                                                                                                  >  1:1484206/1‑150 (MQ=255)
 cGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCAGTGTCGGTCGCGGCTTTCATCGCCACAATAc                                                                                                                                                  >  2:3411501/1‑150 (MQ=255)
  gAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACg                                                                                                                                                 >  1:3965869/1‑150 (MQ=255)
   acaTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACgg                                                                                                                                                <  2:3252191/148‑1 (MQ=255)
   aaaTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACgg                                                                                                                                                >  2:314594/1‑150 (MQ=255)
   aaaTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACgg                                                                                                                                                >  2:3373696/1‑150 (MQ=255)
   aaaTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACgg                                                                                                                                                <  2:790801/150‑1 (MQ=255)
     aTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGggc                                                                                                                                              >  1:1922554/1‑150 (MQ=255)
     aTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGggc                                                                                                                                              >  1:1492381/1‑150 (MQ=255)
     aTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCAGTGTCGGTCGCGGCTTTCATCGCCACAATACGggc                                                                                                                                              >  2:5473/1‑150 (MQ=255)
     aTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGATCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGggc                                                                                                                                              >  1:883520/1‑150 (MQ=255)
        aCCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGggcggc                                                                                                                                           >  1:533483/1‑150 (MQ=255)
         ccTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGcc                                                                                                                                          >  1:2593658/1‑150 (MQ=255)
             tttAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCt                                                                                                                                      >  1:1249688/1‑150 (MQ=255)
              ttAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTc                                                                                                                                     >  2:1340462/1‑150 (MQ=255)
               tAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACTAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCg                                                                                                                                    >  2:1642250/1‑150 (MQ=255)
               tAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCg                                                                                                                                    >  2:2988158/1‑150 (MQ=255)
                aaaCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGc                                                                                                                                   >  1:2472444/1‑150 (MQ=255)
                 aaCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTATTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCt                                                                                                                                  <  1:989414/150‑1 (MQ=255)
                 aaCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTAGACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCt                                                                                                                                  <  2:1570809/150‑1 (MQ=255)
                 aaCCGCGGCGGCCCCCGAGACGATCACGGTGAGTTCCTGAGTAAAGCTGGCCTGACGAGCTATGATCTATACCAACTCCAGCTCTTTAATCAGGCTGACGCCATGCTCGGGCGCGGCATTCATCCCCACAATAAGGGCGGCCTGCTCGCt                                                                                                                                  >  2:4039035/1‑150 (MQ=255)
                  aCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTg                                                                                                                                 <  2:2330683/150‑1 (MQ=255)
                  aCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTg                                                                                                                                 <  1:4093018/150‑1 (MQ=255)
                      cggcggCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCa                                                                                                                             >  1:184485/1‑150 (MQ=255)
                         cggcCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGt                                                                                                                          >  1:3508707/1‑150 (MQ=255)
                          ggcCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGtt                                                                                                                         <  2:2580671/150‑1 (MQ=255)
                          ggcCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGtt                                                                                                                         >  2:3051873/1‑150 (MQ=255)
                          ggcCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGtt                                                                                                                         >  1:1023690/1‑150 (MQ=255)
                          ggcCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTGCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGtt                                                                                                                         >  2:1936673/1‑150 (MQ=255)
                           gcCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGttt                                                                                                                        <  2:3120104/150‑1 (MQ=255)
                            cccccGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGtttt                                                                                                                       >  2:1509168/1‑150 (MQ=255)
                             ccccGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTc                                                                                                                      <  1:1183732/150‑1 (MQ=255)
                              cccGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCa                                                                                                                     >  1:3628395/1‑150 (MQ=255)
                              cccGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCa                                                                                                                     >  1:297656/1‑150 (MQ=255)
                                   gaCGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCAc                                                                                                                <  1:2180049/150‑1 (MQ=255)
                                        tctcGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCt                                                                                                           <  2:3753576/150‑1 (MQ=255)
                                        tctcGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCt                                                                                                           <  2:3448354/150‑1 (MQ=255)
                                           cGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGAt                                                                                                        >  1:578135/1‑150 (MQ=255)
                                            ggTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATa                                                                                                       <  1:3196099/150‑1 (MQ=255)
                                             gTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATaa                                                                                                      >  2:265889/1‑150 (MQ=255)
                                             gTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATaa                                                                                                      >  1:2272798/1‑150 (MQ=255)
                                             gTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATaa                                                                                                      >  2:3437162/1‑150 (MQ=255)
                                             gTGAGGTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATaa                                                                                                      <  1:2784890/150‑1 (MQ=255)
                                              tGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATaaa                                                                                                     >  1:2069694/1‑150 (MQ=255)
                                               gAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAAc                                                                                                    <  2:908294/150‑1 (MQ=255)
                                                 gTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCt                                                                                                  >  1:3050402/1‑150 (MQ=255)
                                                   tCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTga                                                                                                >  1:701094/1‑150 (MQ=255)
                                                   tCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTga                                                                                                <  2:2629620/150‑1 (MQ=255)
                                                    ccTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTgag                                                                                               >  2:3635101/1‑150 (MQ=255)
                                                    ccTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTgag                                                                                               <  1:3898140/150‑1 (MQ=255)
                                                     cTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTgaga                                                                                              >  1:1887962/1‑150 (MQ=255)
                                                      tGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGAt                                                                                             >  2:2359314/1‑150 (MQ=255)
                                                        aGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTc                                                                                           <  2:2802515/150‑1 (MQ=255)
                                                        aGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTc                                                                                           <  2:874332/150‑1 (MQ=255)
                                                        aGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTc                                                                                           <  2:2020553/150‑1 (MQ=255)
                                                           aaTGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGAc                                                                                        >  2:3977876/1‑150 (MQ=255)
                                                               cTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATaa                                                                                    >  2:3202527/1‑150 (MQ=255)
                                                                 ggCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAacg                                                                                  >  2:703815/1‑150 (MQ=255)
                                                                     tGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACgc                                                                              >  1:256934/1‑150 (MQ=255)
                                                                             tttGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCAGATAAACCTGAGATTCGACATAACGACGCAGCAGGGt                                                                      >  2:770544/1‑150 (MQ=255)
                                                                                 ttgtATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATcc                                                                  >  2:349287/1‑150 (MQ=255)
                                                                                   gtATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAg                                                                <  1:3421981/150‑1 (MQ=255)
                                                                                    tataCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGc                                                               >  1:3776943/1‑150 (MQ=255)
                                                                                    tataCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGc                                                               >  1:2847007/1‑150 (MQ=255)
                                                                                    tataCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGc                                                               >  2:30801/1‑150 (MQ=255)
                                                                                       aCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAAc                                                            <  1:3253825/150‑1 (MQ=255)
                                                                                        ccAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACg                                                           <  2:1682025/150‑1 (MQ=255)
                                                                                          aaCTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGcc                                                         >  1:4036185/1‑150 (MQ=255)
                                                                                           aCTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCt                                                        <  2:2199177/150‑1 (MQ=255)
                                                                                             tGCAGCTCTTTAATCAGGCTGCCGCCATTGTCTGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAAACACGCCCTGTTAAACCTGAGATTCGACATTACGACGCAGCAGGGTATCCAGCAACGCCTTc                                                      <  1:1458522/150‑1 (MQ=255)
                                                                                             tGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTc                                                      >  1:3118719/1‑150 (MQ=255)
                                                                                               cAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCgg                                                    >  2:1842270/1‑150 (MQ=255)
                                                                                                 gCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGAt                                                  <  1:2699549/150‑1 (MQ=255)
                                                                                                  ctctTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATc                                                 >  2:2890578/1‑150 (MQ=255)
                                                                                                   tctTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCg                                                <  1:3315608/150‑1 (MQ=255)
                                                                                                       tAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGtt                                            <  1:2534680/150‑1 (MQ=255)
                                                                                                       tAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGtt                                            >  2:3821949/1‑150 (MQ=255)
                                                                                                         aTCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCg                                          <  2:1424289/150‑1 (MQ=255)
                                                                                                         aTCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCg                                          <  2:3730295/150‑1 (MQ=255)
                                                                                                          tCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGt                                         <  1:1823484/150‑1 (MQ=255)
                                                                                                            aGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTAc                                       <  2:2239420/150‑1 (MQ=255)
                                                                                                              gCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAg                                     <  1:3120965/150‑1 (MQ=255)
                                                                                                                tGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGt                                   <  1:1780138/150‑1 (MQ=255)
                                                                                                                 gccgccATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTa                                  <  1:4028574/150‑1 (MQ=255)
                                                                                                                  ccgccATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTaa                                 >  1:2964086/1‑150 (MQ=255)
                                                                                                                   cgccATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAAt                                >  1:2400948/1‑150 (MQ=255)
                                                                                                                       aTTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCa                            <  1:2124613/150‑1 (MQ=255)
                                                                                                                         tGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAgg                          <  2:1349647/150‑1 (MQ=255)
                                                                                                                            cggtcgCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGAtt                       >  1:724786/1‑150 (MQ=255)
                                                                                                                             ggtcgCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGAttt                      >  2:3864783/1‑150 (MQ=255)
                                                                                                                                    gCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTc               <  2:4100334/150‑1 (MQ=255)
                                                                                                                                        tCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGat           >  2:1386644/1‑150 (MQ=255)
                                                                                                                                         cATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGatc          >  2:32634/1‑150 (MQ=255)
                                                                                                                                         cATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGatc          <  1:2548002/150‑1 (MQ=255)
                                                                                                                                          aTCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGatca         >  2:1677445/1‑150 (MQ=255)
                                                                                                                                           tCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGatcat        >  1:3890572/1‑150 (MQ=255)
                                                                                                                                            cGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGatcatc       >  1:978665/1‑150 (MQ=255)
                                                                                                                                              ccacaaTACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGatcatcat     <  2:2128677/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCATCAT  >  CP009273/3910748‑3911042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑