BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,921,331	C→A	6.6%	R273S (CGC→AGC)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,921,331	0	C	A	6.6%	1002.2 / 30.3	258	R273S (CGC→AGC)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base C (125/116); new base A (10/7); total (135/123)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 264 total aligned reads displayed.

CCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTT  >  CP009273/3921182‑3921480

ccccGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATc                                                                                                                                                       <  2:2216991/150‑1 (MQ=255)

  ccGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGc                                                                                                                                                     <  1:2521573/150‑1 (MQ=255)

    gTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCct                                                                                                                                                   >  2:832137/1‑150 (MQ=255)

    gTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCct                                                                                                                                                   <  1:3383510/150‑1 (MQ=255)

     tCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctg                                                                                                                                                  >  1:371632/1‑150 (MQ=255)

       agagCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctgga                                                                                                                                                >  2:2106403/1‑150 (MQ=255)

        gagCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctggag                                                                                                                                               <  1:3937575/150‑1 (MQ=255)

           cTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctggagctg                                                                                                                                            <  2:3989578/150‑1 (MQ=255)

           cTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctggagctg                                                                                                                                            <  2:1915753/150‑1 (MQ=255)

           cTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATAGCctggagctg                                                                                                                                            <  2:3564052/150‑1 (MQ=255)

            tGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctggagctgg                                                                                                                                           >  2:300424/1‑150 (MQ=255)

            tGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctggagctgg                                                                                                                                           >  2:2215335/1‑150 (MQ=255)

             gggCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctggagctgga                                                                                                                                          <  2:229619/150‑1 (MQ=255)

              ggCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGGCGTAGATCGCctggagctggag                                                                                                                                         >  2:3788479/1‑150 (MQ=255)

                ccttGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACAAGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTg                                                                                                                                       <  1:666137/147‑1 (MQ=255)

                   tGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCa                                                                                                                                    <  2:78713/150‑1 (MQ=255)

                       ggTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATAGCCTGGAGCTGGAGTGGCATCAg                                                                                                                                <  1:130821/150‑1 (MQ=255)

                         tCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGgc                                                                                                                              <  2:1927256/150‑1 (MQ=255)

                          cTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGgcg                                                                                                                             <  2:3910381/150‑1 (MQ=255)

                            gAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCgct                                                                                                                           >  2:3919786/1‑150 (MQ=255)

                            gAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCgct                                                                                                                           >  2:2245662/1‑150 (MQ=255)

                            gAACGGCGATATTCTGGTGTGGAAACAGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATACGTTTAGAAGCCTACACGCTGAAGCATCAACTGTCGCTCACCGGTGACGAAGATCGCCTGGAGATGGAGTGGCATCAGGCgct                                                                                                                           >  1:2150605/1‑150 (MQ=255)

                             aaCGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCgctg                                                                                                                          >  2:1806085/1‑150 (MQ=255)

                             aaCGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCgctg                                                                                                                          >  2:941790/1‑150 (MQ=255)

                             aaCGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCgctg                                                                                                                          >  1:2107382/1‑150 (MQ=255)

                             aaCGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCgctg                                                                                                                          >  1:168124/1‑150 (MQ=255)

                               cGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCgctgct                                                                                                                        <  2:1755403/150‑1 (MQ=255)

                                 gCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCtgctgc                                                                                                                      >  1:3561146/1‑150 (MQ=255)

                                  cGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTgcg                                                                                                                     <  2:3991116/150‑1 (MQ=255)

                                     tatTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCgg                                                                                                                  >  2:2866367/1‑150 (MQ=255)

                                     tatTCAGGTGTGGAACCAGGTACTGGAATATGCGTTAGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCTGTGAAGAAGATCTCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCgg                                                                                                                  >  2:197947/1‑150 (MQ=255)

                                         cTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGaa                                                                                                              >  1:1062530/1‑150 (MQ=255)

                                           ggTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAAt                                                                                                            >  1:1900945/1‑150 (MQ=255)

                                             tgtgGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATAGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGc                                                                                                          >  1:201917/1‑150 (MQ=255)

                                                ggAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGc                                                                                                       <  2:2078197/150‑1 (MQ=255)

                                                        gTACTGGAAGATGCGTTTGAGCTTTCCTCCAGGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATc                                                                                               >  2:3204831/1‑150 (MQ=255)

                                                         tACTTGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATcg                                                                                              <  2:458461/150‑1 (MQ=255)

                                                         tACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATcg                                                                                              >  1:3724158/1‑150 (MQ=255)

                                                           cTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATAGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATcggc                                                                                            >  1:3489679/1‑150 (MQ=255)

                                                            tGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATcggcg                                                                                           >  2:1469979/1‑150 (MQ=255)

                                                                 gATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGt                                                                                      >  2:132386/1‑150 (MQ=255)

                                                                 gATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGt                                                                                      >  2:1459491/1‑150 (MQ=255)

                                                                  aTGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTa                                                                                     >  2:2559011/1‑150 (MQ=255)

                                                                   tGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTAt                                                                                    <  2:3092305/150‑1 (MQ=255)

                                                                      gTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATAGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCgg                                                                                 <  1:890744/150‑1 (MQ=255)

                                                                         tGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCa                                                                              <  2:3364678/150‑1 (MQ=255)

                                                                         tGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCa                                                                              >  2:4030203/1‑150 (MQ=255)

                                                                          gAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAg                                                                             >  1:2776452/1‑150 (MQ=255)

                                                                          gAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAg                                                                             >  1:2951708/1‑150 (MQ=255)

                                                                           aGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGt                                                                            >  1:2305152/1‑150 (MQ=255)

                                                                             cTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGtct                                                                          <  2:1137921/150‑1 (MQ=255)

                                                                                 cctccATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGtt                                                                      <  2:1877172/150‑1 (MQ=255)

                                                                                    ccATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGa                                                                   >  1:709926/1‑150 (MQ=255)

                                                                                      aTGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACt                                                                 <  2:2989605/150‑1 (MQ=255)

                                                                                      aTGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACt                                                                 <  2:2610088/150‑1 (MQ=255)

                                                                                        ggggATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTAt                                                               >  1:1109902/1‑150 (MQ=255)

                                                                                          ggATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTAtgc                                                             >  1:916621/1‑150 (MQ=255)

                                                                                           gATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTAtgct                                                            >  1:3482849/1‑150 (MQ=255)

                                                                                              ccGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTAtgctgct                                                         <  2:1125825/150‑1 (MQ=255)

                                                                                                 tgtAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTAtgctgctgct                                                      <  2:100711/150‑1 (MQ=255)

                                                                                                  gtAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTAtgctgctgctg                                                     <  2:2837110/150‑1 (MQ=255)

                                                                                                   tAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTAtgctgctgctgc                                                    >  1:3176799/1‑150 (MQ=255)

                                                                                                   tAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTAtgctgctgctgc                                                    <  2:2258739/150‑1 (MQ=255)

                                                                                                    aGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCa                                                   >  1:1457159/1‑150 (MQ=255)

                                                                                                    aGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCa                                                   >  1:1456702/1‑150 (MQ=255)

                                                                                                    aGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCa                                                   <  1:416292/150‑1 (MQ=255)

                                                                                                    aGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCa                                                   <  1:1119454/150‑1 (MQ=255)

                                                                                                       tGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACt                                                >  1:287076/1‑150 (MQ=255)

                                                                                                       tGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACt                                                <  1:2496286/150‑1 (MQ=255)

                                                                                                         ccGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGc                                              >  1:1058720/1‑150 (MQ=255)

                                                                                                              acGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCat                                         >  2:1631262/1‑150 (MQ=255)

                                                                                                               cGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCata                                        <  2:3029323/150‑1 (MQ=255)

                                                                                                                gCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCatat                                       <  2:1652908/150‑1 (MQ=255)

                                                                                                                 cTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATc                                      <  2:2056893/150‑1 (MQ=255)

                                                                                                                  tGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTCTCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCCTATCg                                     >  2:1862826/1‑150 (MQ=255)

                                                                                                                       cATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAg                                >  2:2606708/1‑150 (MQ=255)

                                                                                                                        aTCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAgg                               >  2:3683869/1‑150 (MQ=255)

                                                                                                                          cAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGtt                             >  2:550690/1‑150 (MQ=255)

                                                                                                                            aCTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCa                           >  1:2342536/1‑150 (MQ=255)

                                                                                                                             cTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAg                          <  1:2053946/150‑1 (MQ=255)

                                                                                                                             cTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAg                          <  2:3601548/150‑1 (MQ=255)

                                                                                                                             cTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAg                          >  2:2140764/1‑150 (MQ=255)

                                                                                                                              tGGCGCTGACCGGTGACGAAGATAGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAgt                         >  1:1296478/1‑150 (MQ=255)

                                                                                                                               ggCGCTGAGCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAgtg                        <  2:4062815/150‑1 (MQ=255)

                                                                                                                                   cTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGa                    <  1:1644120/150‑1 (MQ=255)

                                                                                                                                   cTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGa                    <  1:1785949/150‑1 (MQ=255)

                                                                                                                                    tGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAg                   >  1:2622367/1‑150 (MQ=255)

                                                                                                                                     gACCGGTGACGACGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGt                  <  1:820211/150‑1 (MQ=255)

                                                                                                                                     gACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGt                  <  1:820041/150‑1 (MQ=255)

                                                                                                                                      aCCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTa                 <  1:616677/150‑1 (MQ=255)

                                                                                                                                        cGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATg               >  2:2328758/1‑150 (MQ=255)

                                                                                                                                         ggTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATgg              >  1:78438/1‑150 (MQ=255)

                                                                                                                                         ggTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATgg              <  1:841563/150‑1 (MQ=255)

                                                                                                                                          gTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGc             <  1:243561/150‑1 (MQ=255)

                                                                                                                                           tGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGcc            >  2:3871749/1‑150 (MQ=255)

                                                                                                                                               gAAGATCGCCTGGAGCTGGAGTTGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAgct        <  1:1266658/150‑1 (MQ=255)

                                                                                                                                               gAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAgct        <  1:1266393/150‑1 (MQ=255)

                                                                                                                                                  gATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAgctgct     >  2:2742416/1‑150 (MQ=255)

                                                                                                                                                   aTCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGctgctg    <  2:3833366/150‑1 (MQ=255)

                                                                                                                                                     cGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGtt  <  1:1208169/150‑1 (MQ=255)

CCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTT  >  CP009273/3921182‑3921480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`