BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,926,536	G→T	8.7%	E621* (GAA→TAA)	kup →	potassium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,926,536	0	G	T	8.7%	869.0 / 38.4	229	E621* (GAA→TAA)	kup	potassium transporter
Reads supporting (aligned to +/- strand): ref base G (86/123); new base T (5/15); total (91/138)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 234 total aligned reads displayed.

TGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCG  >  CP009273/3926387‑3926685

tGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCg                                                                                                                                                       <  1:487160/150‑1 (MQ=255)

  tCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGaa                                                                                                                                                     >  1:3047199/1‑150 (MQ=255)

    gCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAAt                                                                                                                                                   <  1:1876351/150‑1 (MQ=255)

      aTGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCt                                                                                                                                                 <  2:1724947/150‑1 (MQ=255)

          gTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACg                                                                                                                                             >  2:2049689/1‑150 (MQ=255)

                gATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGAc                                                                                                                                       <  1:3135430/150‑1 (MQ=255)

                gATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGAc                                                                                                                                       <  1:765516/150‑1 (MQ=255)

                gATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGAc                                                                                                                                       >  2:667482/1‑150 (MQ=255)

                   ccTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGcc                                                                                                                                    <  1:1639609/150‑1 (MQ=255)

                       ggCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCTAAATCTAACGCCAGACGcctcct                                                                                                                                >  1:167261/1‑150 (MQ=255)

                       ggCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGcctcct                                                                                                                                >  2:3087631/1‑150 (MQ=255)

                       ggCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGcctcct                                                                                                                                >  1:1165325/1‑150 (MQ=255)

                         cAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCttt                                                                                                                              <  2:2914753/150‑1 (MQ=255)

                          aaaCGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTttc                                                                                                                             <  2:2275107/150‑1 (MQ=255)

                           aaCGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTttct                                                                                                                            <  2:2690878/150‑1 (MQ=255)

                           aaCGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTttct                                                                                                                            >  2:2095677/1‑150 (MQ=255)

                              gCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCa                                                                                                                         <  2:321327/150‑1 (MQ=255)

                               cccGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCAt                                                                                                                        <  2:2949794/150‑1 (MQ=255)

                                ccGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATa                                                                                                                       <  2:986590/150‑1 (MQ=255)

                                ccGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATa                                                                                                                       <  2:1939894/150‑1 (MQ=255)

                                    ggTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAAAGCCAGACGCCTCCTTTCTTCATAAggg                                                                                                                   <  2:130334/150‑1 (MQ=255)

                                      tATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAggggg                                                                                                                 >  1:1359733/1‑150 (MQ=255)

                                      tATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAggggg                                                                                                                 >  2:739088/1‑150 (MQ=255)

                                        tttGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCg                                                                                                               >  2:3347272/1‑150 (MQ=255)

                                           gCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGttt                                                                                                            <  1:681564/150‑1 (MQ=255)

                                           gCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGttt                                                                                                            >  2:1972345/1‑150 (MQ=255)

                                             gTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGttttt                                                                                                          <  1:2684703/150‑1 (MQ=255)

                                             gTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGttttt                                                                                                          >  1:3153263/1‑150 (MQ=255)

                                             gTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGttttt                                                                                                          <  1:3430532/150‑1 (MQ=255)

                                              tCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCTAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTg                                                                                                         <  2:1958589/150‑1 (MQ=255)

                                              tCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTg                                                                                                         <  2:1992713/150‑1 (MQ=255)

                                                tGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCTAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGtt                                                                                                       <  1:63333/150‑1 (MQ=255)

                                                  cgcgGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACATGGTTATCGAACTGGGTACTCAGGTCTAAATCTAACTCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGtttt                                                                                                     <  2:1874726/150‑1 (MQ=255)

                                                  cgcgGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGtttt                                                                                                     >  1:1418418/1‑150 (MQ=255)

                                                    cgGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCa                                                                                                   <  1:4145273/150‑1 (MQ=255)

                                                     ggCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCAt                                                                                                  <  2:1108241/150‑1 (MQ=255)

                                                     ggCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCAt                                                                                                  <  2:1341984/150‑1 (MQ=255)

                                                     ggCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCAt                                                                                                  <  2:144281/150‑1 (MQ=255)

                                                     ggCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTAAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTGTCGTCATAAGGGGGCGTTTTTGTTTTCAt                                                                                                  >  1:995293/1‑150 (MQ=255)

                                                      gCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATg                                                                                                 >  2:1806665/1‑150 (MQ=255)

                                                       cAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATgg                                                                                                >  1:2756175/1‑150 (MQ=255)

                                                       cAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATgg                                                                                                <  2:3633269/150‑1 (MQ=255)

                                                        aaGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGt                                                                                               >  1:3650067/1‑150 (MQ=255)

                                                        aaGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGt                                                                                               >  1:2807546/1‑150 (MQ=255)

                                                        aaGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGt                                                                                               <  1:1577277/150‑1 (MQ=255)

                                                          gCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCTAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTa                                                                                             <  1:817376/150‑1 (MQ=255)

                                                            tGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAAt                                                                                           >  1:2372045/1‑150 (MQ=255)

                                                            tGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAAt                                                                                           <  2:1854869/150‑1 (MQ=255)

                                                            tGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAAt                                                                                           <  2:4034153/150‑1 (MQ=255)

                                                             gTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATc                                                                                          >  2:3301658/1‑150 (MQ=255)

                                                              tACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCa                                                                                         >  1:2051522/1‑150 (MQ=255)

                                                                 ttGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCa                                                                                      <  2:2828124/150‑1 (MQ=255)

                                                                      gctgcAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTaa                                                                                 <  2:1281557/150‑1 (MQ=255)

                                                                      gctgcAACGTAATGCGCTGCGTGCACCAGATCAATGTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGTTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTaa                                                                                 <  2:917175/150‑1 (MQ=255)

                                                                            aCGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGtt                                                                           <  2:2889900/150‑1 (MQ=255)

                                                                            aCGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGtt                                                                           <  1:2960942/150‑1 (MQ=255)

                                                                              gTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCTAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTc                                                                         <  1:5410/150‑1 (MQ=255)

                                                                              gTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTc                                                                         <  1:916598/150‑1 (MQ=255)

                                                                              gTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTc                                                                         <  2:2849815/150‑1 (MQ=255)

                                                                              gTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTc                                                                         <  1:3607839/150‑1 (MQ=255)

                                                                                aaTGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGa                                                                       >  1:581460/1‑150 (MQ=255)

                                                                                    cgcTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGtt                                                                   <  2:594490/150‑1 (MQ=255)

                                                                                     gcTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTg                                                                  <  1:2644162/150‑1 (MQ=255)

                                                                                           tGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATcaca                                                            <  1:274399/150‑1 (MQ=255)

                                                                                           tGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATcaca                                                            <  1:2891325/150‑1 (MQ=255)

                                                                                            gCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACAt                                                           <  2:710410/150‑1 (MQ=255)

                                                                                            gCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGGTGATCACAt                                                           >  1:3726452/1‑150 (MQ=255)

                                                                                             cACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCTAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACAtt                                                          >  1:1792006/1‑150 (MQ=255)

                                                                                             cACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACAtt                                                          >  1:2955264/1‑150 (MQ=255)

                                                                                             cACCAGATCAATTTGAAATCACGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTGTTTGTTTTAATGGTTAATCACCAAGTAAAACGTTTAGAGGTTGATcacaat                                                          >  1:2865204/1‑148 (MQ=255)

                                                                                              aCCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACAttt                                                         <  1:950392/150‑1 (MQ=255)

                                                                                               ccAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGGTTCGAGGTTGATCACATTTc                                                        >  2:3844575/1‑150 (MQ=255)

                                                                                                cAGATCAATTTGAAATCCCGCCACACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTcc                                                       <  2:2107559/150‑1 (MQ=255)

                                                                                                cAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTcc                                                       <  2:2709314/150‑1 (MQ=255)

                                                                                                   aTCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCTAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTGTTTGTTTTCATGGTTAATCACCATGTAAAACTTTTCGAGGTTGATCACATTTCCGTa                                                    >  1:1013103/1‑150 (MQ=255)

                                                                                                   aTCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTa                                                    >  1:1499002/1‑150 (MQ=255)

                                                                                                    tCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCTAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTaa                                                   <  2:2268260/150‑1 (MQ=255)

                                                                                                           gAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACg                                            <  2:2516101/150‑1 (MQ=255)

                                                                                                            aaaTCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGGTTCGAGGTTGATCACATTTCCGTAACGTCACGa                                           >  1:1271712/1‑150 (MQ=255)

                                                                                                             aaTCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATCAGGGGGCGTTTTTTTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTGGATCAAATTTCCGTAACGTCACGAt                                          >  2:162798/1‑150 (MQ=255)

                                                                                                             aaTCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGAt                                          >  1:1463401/1‑150 (MQ=255)

                                                                                                              aTCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATg                                         <  2:3783229/150‑1 (MQ=255)

                                                                                                                cccGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCTAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGt                                       >  2:4127797/1‑150 (MQ=255)

                                                                                                                cccGCCAAACAGGGTTATCGAACTGGGTACTCAGGTAGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGt                                       <  1:3960810/150‑1 (MQ=255)

                                                                                                                 ccGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGtt                                      <  2:1523582/150‑1 (MQ=255)

                                                                                                                 ccGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGtt                                      <  1:232792/150‑1 (MQ=255)

                                                                                                                   gCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGtttt                                    <  2:3948428/150‑1 (MQ=255)

                                                                                                                      aaaCAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTAATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTccc                                 >  1:886220/1‑150 (MQ=255)

                                                                                                                       aaCAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCa                                >  1:2442476/1‑150 (MQ=255)

                                                                                                                        aCAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCaa                               <  2:559209/150‑1 (MQ=255)

                                                                                                                         cAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAAc                              >  2:1827038/1‑150 (MQ=255)

                                                                                                                              ttATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACtcagt                         >  2:370803/1‑150 (MQ=255)

                                                                                                                                       tGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTaa                >  1:1283170/1‑150 (MQ=255)

                                                                                                                                          gTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACt             <  1:2845235/150‑1 (MQ=255)

                                                                                                                                                 ggTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTc      >  2:3411479/1‑150 (MQ=255)

                                                                                                                                                 ggTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTc      <  1:3194135/150‑1 (MQ=255)

                                                                                                                                                 ggTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTc      <  1:327541/150‑1 (MQ=255)

                                                                                                                                                  gTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCa     <  1:3742596/150‑1 (MQ=255)

                                                                                                                                                     gAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCg  >  1:2662511/1‑150 (MQ=255)

                                                                                                                                                     gAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTGTTTGTTTTCATGGTTAATCACCCTGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGGCAGCg  >  1:1084323/1‑150 (MQ=255)

TGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACGCCAGACGCCTCCTTTCTTCATAAGGGGGCGTTTTTGTTTTCATGGTTAATCACCATGTAAAACGTTTCGAGGTTGATCACATTTCCGTAACGTCACGATGGTTTTCCCAACTCAGTCAGGATTAAACTGTGGGTCAGCG  >  CP009273/3926387‑3926685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`