Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,000,333 G→T 6.9% A370A (GCG→GCT)  recQ → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,000,3330GT 6.9% 1241.2 / 36.5 322A370A (GCG→GCT) recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (158/140);  new base T (15/9);  total (173/149)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 329 total aligned reads displayed.

TATCAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCT  >  CP009273/4000184‑4000482
                                                                                                                                                     |                                                                                                                                                     
tatCAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCg                                                                                                                                                       <  1:2042173/150‑1 (MQ=255)
tatCAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCg                                                                                                                                                       >  1:1971569/1‑150 (MQ=255)
 atCAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGa                                                                                                                                                      >  2:3847848/1‑150 (MQ=255)
 atCAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGa                                                                                                                                                      >  2:3418987/1‑150 (MQ=255)
 atCAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGa                                                                                                                                                      >  1:1881937/1‑150 (MQ=255)
   cAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATg                                                                                                                                                    <  2:1525924/150‑1 (MQ=255)
    aGGAAACCGGACGCGCCGGGCGTGATGGCCTTCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATgg                                                                                                                                                   >  1:2938830/1‑150 (MQ=255)
    aGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATgg                                                                                                                                                   >  1:3063733/1‑150 (MQ=255)
       aaaCCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGgcg                                                                                                                                                <  1:3756684/150‑1 (MQ=255)
        aaCCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGgcgc                                                                                                                                               >  2:2763097/1‑150 (MQ=255)
        aaCCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGgcgc                                                                                                                                               >  1:195586/1‑150 (MQ=255)
         aCCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGcgcg                                                                                                                                              >  1:1550048/1‑150 (MQ=255)
           cGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCAGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCTATGCGATGGGCGCGtt                                                                                                                                            >  2:2802300/1‑150 (MQ=255)
            ggACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGttt                                                                                                                                           >  1:2793319/1‑150 (MQ=255)
            ggACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGttt                                                                                                                                           >  1:458389/1‑150 (MQ=255)
             gACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCTATGGGCGCGTTTg                                                                                                                                          <  2:1289959/150‑1 (MQ=255)
             gACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCTATGGGCGCGTTTg                                                                                                                                          >  1:2354391/1‑150 (MQ=255)
             gACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTg                                                                                                                                          <  1:653430/150‑1 (MQ=255)
                         gTGATGGCCTGCCCGCGGAAGCGGTGCTGTTTTACGATCCGGCTGTTATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCaaa                                                                                                                              >  1:2768804/1‑150 (MQ=255)
                         gTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCaaa                                                                                                                              >  2:2448430/1‑150 (MQ=255)
                            aTGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACtt                                                                                                                           >  2:734639/1‑150 (MQ=255)
                            aTGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACtt                                                                                                                           <  2:525782/150‑1 (MQ=255)
                                ccTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCcg                                                                                                                       <  1:3892194/150‑1 (MQ=255)
                                 cTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCcgt                                                                                                                      >  1:34233/1‑150 (MQ=255)
                                  tGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCcgtc                                                                                                                     >  1:4082258/1‑150 (MQ=255)
                                  tGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCcgtc                                                                                                                     <  2:3513651/150‑1 (MQ=255)
                                  tGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCcgtc                                                                                                                     <  2:4022360/150‑1 (MQ=255)
                                     ccGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCgtcgtc                                                                                                                  <  2:2425050/150‑1 (MQ=255)
                                       gcgGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCTATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTg                                                                                                                <  1:1036687/150‑1 (MQ=255)
                                       gcgGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTg                                                                                                                <  1:3221564/150‑1 (MQ=255)
                                         ggAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCTATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGt                                                                                                              >  1:1022793/1‑150 (MQ=255)
                                         ggAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCTATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGt                                                                                                              >  1:515473/1‑150 (MQ=255)
                                         ggAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGt                                                                                                              <  2:1713810/150‑1 (MQ=255)
                                          gAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTa                                                                                                             >  1:1299255/1‑150 (MQ=255)
                                          gAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTa                                                                                                             >  1:2670878/1‑150 (MQ=255)
                                            aGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTAtt                                                                                                           >  2:1006322/1‑150 (MQ=255)
                                              cGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCTATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGc                                                                                                         >  2:4096378/1‑150 (MQ=255)
                                               gATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCt                                                                                                        >  2:3274770/1‑150 (MQ=255)
                                                aTGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTg                                                                                                       <  1:107195/150‑1 (MQ=255)
                                                 tGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGa                                                                                                      >  2:1972717/1‑150 (MQ=255)
                                                 tGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGa                                                                                                      >  1:846834/1‑150 (MQ=255)
                                                    tGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACt                                                                                                   <  1:3591410/150‑1 (MQ=255)
                                                     gTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCTATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTa                                                                                                  >  2:1427185/1‑150 (MQ=255)
                                                        ttACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTAttt                                                                                               <  2:3450456/150‑1 (MQ=255)
                                                           cGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCTATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTgg                                                                                            >  2:3168414/1‑150 (MQ=255)
                                                             aTCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCg                                                                                          >  1:961423/1‑150 (MQ=255)
                                                              tCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGa                                                                                         <  2:2924312/150‑1 (MQ=255)
                                                              tCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGa                                                                                         >  1:1769612/1‑150 (MQ=255)
                                                                   cTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATAGCTGAACTATTGTGGCGACGGGc                                                                                    >  1:1059086/1‑150 (MQ=255)
                                                                    tGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCg                                                                                   >  2:3763276/1‑150 (MQ=255)
                                                                    tGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCg                                                                                   >  1:1739892/1‑150 (MQ=255)
                                                                    tGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCg                                                                                   >  1:2388878/1‑150 (MQ=255)
                                                                    tGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCg                                                                                   >  2:1599356/1‑150 (MQ=255)
                                                                    tGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCg                                                                                   >  1:3735109/1‑150 (MQ=255)
                                                                     gATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGt                                                                                  >  2:3585507/1‑150 (MQ=255)
                                                                     gATATGGCGTGGCTGCGCCGTTGTCGTGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGTGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCGGGTATTGCTGAACTATTTTGGCGAAGGGCGt                                                                                  >  2:2474264/1‑150 (MQ=255)
                                                                       tatGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCTATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCa                                                                                <  1:7767/150‑1 (MQ=255)
                                                                       tatGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCa                                                                                >  2:2499464/1‑150 (MQ=255)
                                                                         tGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCTATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAgg                                                                              <  2:2608432/150‑1 (MQ=255)
                                                                          ggCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGa                                                                             <  1:1142144/150‑1 (MQ=255)
                                                                          ggCGTGGCAGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGGGGTGTGGTATTGCTGAACTATTGTGGCGAAGGGCGTCAggt                                                                             >  2:2590110/1‑149 (MQ=255)
                                                                            cGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGc                                                                           >  2:2277493/1‑150 (MQ=255)
                                                                             gTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGcc                                                                          <  1:1354720/150‑1 (MQ=255)
                                                                               ggCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGt                                                                        >  1:3988884/1‑150 (MQ=255)
                                                                               ggCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGt                                                                        >  1:1222894/1‑150 (MQ=255)
                                                                                     gcCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGa                                                                  >  1:3467616/1‑150 (MQ=255)
                                                                                      ccGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGaa                                                                 <  2:2101616/150‑1 (MQ=255)
                                                                                       cGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAAc                                                                <  2:1551425/150‑1 (MQ=255)
                                                                                       cGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAAc                                                                >  2:2742526/1‑150 (MQ=255)
                                                                                        gTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCTATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACt                                                               >  2:3386288/1‑150 (MQ=255)
                                                                                          tGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGc                                                             <  1:2797613/150‑1 (MQ=255)
                                                                                            tCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGa                                                           >  2:769284/1‑150 (MQ=255)
                                                                                               ggAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGatat                                                        <  1:3680291/150‑1 (MQ=255)
                                                                                                 aaGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCt                                                      >  1:989609/1‑150 (MQ=255)
                                                                                                      aaGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTc                                                 <  2:3857606/150‑1 (MQ=255)
                                                                                                       aGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCg                                                <  2:683144/150‑1 (MQ=255)
                                                                                                          cGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATc                                             >  1:2152065/1‑150 (MQ=255)
                                                                                                          cGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATc                                             >  2:3009767/1‑150 (MQ=255)
                                                                                                                ggCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGa                                       >  1:4143461/1‑150 (MQ=255)
                                                                                                                   aGTTGCAGGATATCGAGCGCCACAAACTCACTGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAAc                                    <  2:3089696/150‑1 (MQ=255)
                                                                                                                      tGCAGGATATCGAGCGCCACAAACTCAATGNGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGt                                 >  1:3854158/1‑150 (MQ=255)
                                                                                                                       gCAGGATATCGAGCGCNACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGACCTGCGATATCTGCCTCGATCCGCCGAAACAGTa                                >  2:2253554/1‑150 (MQ=255)
                                                                                                                               tCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGtt                        <  2:2325528/150‑1 (MQ=255)
                                                                                                                               tCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGtt                        <  1:3462439/150‑1 (MQ=255)
                                                                                                                               tCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGtt                        >  1:3367286/1‑150 (MQ=255)
                                                                                                                               tCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCAGTAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTTCGACGttt                        <  2:875247/150‑1 (MQ=255)
                                                                                                                                  aGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCaa                     >  1:3575626/1‑150 (MQ=255)
                                                                                                                                    cgcCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAAcc                   <  1:435535/150‑1 (MQ=255)
                                                                                                                                     gcCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCg                  >  2:3646969/1‑150 (MQ=255)
                                                                                                                                      ccACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGa                 >  1:2453336/1‑150 (MQ=255)
                                                                                                                                      ccACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGa                 <  1:3747290/150‑1 (MQ=255)
                                                                                                                                       cacaAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAATCTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGAt                <  1:1455239/150‑1 (MQ=255)
                                                                                                                                       cacaAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGAt                >  1:3190227/1‑150 (MQ=255)
                                                                                                                                        acaAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATg               >  1:4018449/1‑150 (MQ=255)
                                                                                                                                          aaaCTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCt             >  2:2040609/1‑150 (MQ=255)
                                                                                                                                             cTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAg          >  1:1443043/1‑150 (MQ=255)
                                                                                                                                               cAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGAt        <  1:3479973/150‑1 (MQ=255)
                                                                                                                                                aaTGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGAtt       <  2:417659/150‑1 (MQ=255)
                                                                                                                                                     gATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCt  <  2:1128301/150‑1 (MQ=255)
                                                                                                                                                     gATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCt  >  1:3449884/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TATCAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCT  >  CP009273/4000184‑4000482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑