Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,068,208 C→A 100% G60G (GGC→GGA)  yihW → DeoR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,068,2080CA99.6% 854.7 / ‑4.6 238G60G (GGC→GGA) yihWDeoR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (121/116);  minor base T (0/1);  total (121/117)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 239 total aligned reads displayed.

GCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGA  >  CP009273/4068061‑4068354
                                                                                                                                                   |                                                                                                                                                  
   cGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCggg                                                                                                                                               <  1:372218/150‑1 (MQ=255)
    gACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGt                                                                                                                                              >  1:645239/1‑150 (MQ=255)
    gACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGt                                                                                                                                              >  1:2055417/1‑150 (MQ=255)
    gACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGt                                                                                                                                              >  1:1603565/1‑150 (MQ=255)
    gACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCAGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGt                                                                                                                                              >  2:2035208/1‑150 (MQ=255)
     aCCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTc                                                                                                                                             <  1:471577/150‑1 (MQ=255)
      ccAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCg                                                                                                                                            <  2:834873/150‑1 (MQ=255)
        aaCTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCggg                                                                                                                                          >  1:787210/1‑150 (MQ=255)
          ctcctcATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCt                                                                                                                                        <  2:1539720/150‑1 (MQ=255)
              tcATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCa                                                                                                                                    <  1:3998530/150‑1 (MQ=255)
              tcATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCa                                                                                                                                    <  2:1349196/150‑1 (MQ=255)
               cATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAg                                                                                                                                   <  1:1907795/150‑1 (MQ=255)
               cATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAg                                                                                                                                   >  2:3543506/1‑150 (MQ=255)
               cATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAg                                                                                                                                   <  2:1454084/150‑1 (MQ=255)
                aTGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGc                                                                                                                                  >  1:2133649/1‑150 (MQ=255)
                aTGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGc                                                                                                                                  >  2:1730154/1‑150 (MQ=255)
                aTGCTGATCGCCGAGAGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATTTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGTAGCGGGTCGGGCTTCCAGc                                                                                                                                  >  2:1729830/1‑150 (MQ=255)
                     gATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGcgtcgt                                                                                                                             >  2:2918570/1‑150 (MQ=255)
                        cGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTaa                                                                                                                          <  1:943389/150‑1 (MQ=255)
                          ccGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATa                                                                                                                        >  2:644436/1‑150 (MQ=255)
                            gAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACg                                                                                                                      >  1:3499889/1‑150 (MQ=255)
                             aGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACgg                                                                                                                     >  2:970013/1‑150 (MQ=255)
                                 tGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGtt                                                                                                                 <  2:3450317/150‑1 (MQ=255)
                                 tGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGtt                                                                                                                 >  1:3556600/1‑150 (MQ=255)
                                  gggTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTc                                                                                                                <  2:3637366/150‑1 (MQ=255)
                                   ggTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCg                                                                                                               >  1:397549/1‑150 (MQ=255)
                                    gTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGa                                                                                                              >  1:3067157/1‑150 (MQ=255)
                                      atatGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGagc                                                                                                            <  1:2058368/150‑1 (MQ=255)
                                       tatGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGagca                                                                                                           <  1:1141231/150‑1 (MQ=255)
                                          gAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCg                                                                                                        >  2:441461/1‑150 (MQ=255)
                                           aaTATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGt                                                                                                       <  2:4121582/150‑1 (MQ=255)
                                            atatTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTg                                                                                                      <  1:2509852/150‑1 (MQ=255)
                                               ttGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAgg                                                                                                   <  2:2496257/150‑1 (MQ=255)
                                                   tgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTc                                                                                               <  2:3516965/150‑1 (MQ=255)
                                                     aGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGc                                                                                             >  1:2864963/1‑150 (MQ=255)
                                                       cTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCaa                                                                                           >  2:2578223/1‑150 (MQ=255)
                                                        tGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCaaa                                                                                          >  1:3979042/1‑150 (MQ=255)
                                                        tGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCaaa                                                                                          >  1:2432427/1‑150 (MQ=255)
                                                             aaTCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAg                                                                                     >  2:1658978/1‑150 (MQ=255)
                                                             aaTCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAg                                                                                     >  2:1492193/1‑150 (MQ=255)
                                                                ctgctgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaa                                                                                  <  1:813026/150‑1 (MQ=255)
                                                                 tgctgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaaa                                                                                 <  2:3177595/150‑1 (MQ=255)
                                                                    tgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGacaaaa                                                                              >  1:3647694/1‑150 (MQ=255)
                                                                    tgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaaaaaa                                                                              >  2:2800364/1‑150 (MQ=255)
                                                                     ggATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCCAACCGAGGaaaaaaa                                                                             >  2:700558/1‑150 (MQ=255)
                                                                       aTGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAg                                                                           >  2:2865347/1‑150 (MQ=255)
                                                                        tGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGc                                                                          >  1:1000323/1‑150 (MQ=255)
                                                                         gTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCg                                                                         <  2:1804553/150‑1 (MQ=255)
                                                                           ctcCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGAt                                                                       <  1:3192022/150‑1 (MQ=255)
                                                                           ctcCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGAt                                                                       >  1:1212213/1‑150 (MQ=255)
                                                                            tcCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGAtt                                                                      <  2:1964139/150‑1 (MQ=255)
                                                                                   aGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAg                                                               <  2:2116017/150‑1 (MQ=255)
                                                                                   aGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAg                                                               >  2:4088690/1‑150 (MQ=255)
                                                                                   aGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAg                                                               <  1:2669792/150‑1 (MQ=255)
                                                                                     aCGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCg                                                             <  1:3765359/150‑1 (MQ=255)
                                                                                           cgccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCa                                                       >  2:86064/1‑150 (MQ=255)
                                                                                           cgccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCa                                                       >  2:3840490/1‑150 (MQ=255)
                                                                                           cgccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCa                                                       <  1:3476174/150‑1 (MQ=255)
                                                                                            gccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAg                                                      >  1:2964467/1‑150 (MQ=255)
                                                                                            gccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAg                                                      >  1:2964478/1‑150 (MQ=255)
                                                                                             ccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGa                                                     >  2:3917178/1‑150 (MQ=255)
                                                                                              cgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGAc                                                    >  2:2752377/1‑150 (MQ=255)
                                                                                              cgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGAc                                                    >  2:2474485/1‑150 (MQ=255)
                                                                                                ggATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACta                                                  >  2:4167968/1‑150 (MQ=255)
                                                                                                 gATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACtat                                                 <  1:1851692/150‑1 (MQ=255)
                                                                                                 gATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACtat                                                 >  1:156969/1‑150 (MQ=255)
                                                                                                 gATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACtat                                                 <  2:2651628/150‑1 (MQ=255)
                                                                                                   tatTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTatat                                               <  2:1287141/150‑1 (MQ=255)
                                                                                                     ttCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTc                                             <  2:2337803/150‑1 (MQ=255)
                                                                                                       cGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCt                                           <  1:2723598/150‑1 (MQ=255)
                                                                                                          aaaTTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGAt                                        >  1:3340766/1‑150 (MQ=255)
                                                                                                               aaGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTc                                   <  2:616912/150‑1 (MQ=255)
                                                                                                               aaGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTc                                   <  2:3343198/150‑1 (MQ=255)
                                                                                                                  cGAGCATGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCTTCTTTAATACGGCTTTCTTGATGCGTGAGGTTTCGCAAACCTAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaac                                <  2:3733750/150‑1 (MQ=255)
                                                                                                                  cGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaac                                <  1:2284728/150‑1 (MQ=255)
                                                                                                                  cGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGAGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGTAGCGGTGGCAGACTATATTCCTGATGGTTcaac                                <  2:1073729/150‑1 (MQ=255)
                                                                                                                   gAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaaca                               >  2:3967678/1‑150 (MQ=255)
                                                                                                                      cAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAata                            <  2:2964478/150‑1 (MQ=255)
                                                                                                                      cAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAata                            >  2:3282156/1‑150 (MQ=255)
                                                                                                                       aaGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAatat                           >  2:1120597/1‑150 (MQ=255)
                                                                                                                         ggCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATAttt                         <  2:928429/150‑1 (MQ=255)
                                                                                                                            cTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATc                      <  1:3913936/150‑1 (MQ=255)
                                                                                                                            cTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATc                      <  2:3034900/150‑1 (MQ=255)
                                                                                                                            cTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATc                      <  1:2206022/150‑1 (MQ=255)
                                                                                                                             tGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCa                     >  1:418347/1‑150 (MQ=255)
                                                                                                                             tGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCa                     >  1:3402242/1‑150 (MQ=255)
                                                                                                                             tGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCa                     >  1:2278668/1‑150 (MQ=255)
                                                                                                                               aTTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCAcc                   <  1:3129453/150‑1 (MQ=255)
                                                                                                                                  aCGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCAtt                <  2:213332/150‑1 (MQ=255)
                                                                                                                                  aCGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCAtt                <  1:1932753/150‑1 (MQ=255)
                                                                                                                                      gcCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTa            <  1:4116830/150‑1 (MQ=255)
                                                                                                                                      gcCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTa            >  1:1108145/1‑150 (MQ=255)
                                                                                                                                       ccATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTAc           >  1:1509810/1‑150 (MQ=255)
                                                                                                                                         aTCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGa         >  2:230922/1‑150 (MQ=255)
                                                                                                                                          tCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGAc        >  1:3238270/1‑150 (MQ=255)
                                                                                                                                          tCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGAc        <  1:1331695/150‑1 (MQ=255)
                                                                                                                                           cACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATGACCATTGGTACGACt       <  1:3749818/150‑1 (MQ=255)
                                                                                                                                           cACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACt       <  2:3820638/150‑1 (MQ=255)
                                                                                                                                            aCGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTg      <  1:2223977/150‑1 (MQ=255)
                                                                                                                                               gTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTg   <  1:2876205/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGA  >  CP009273/4068061‑4068354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑