Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP009273	3,841,409	+CAACCAG	14.9%	coding (576/1320 nt)	uhpC ←	membrane protein regulates uhpT expression

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 3841409	200 (0.820)	36 (0.140) +CAACCAG	9/244	NT	14.9%	coding (576/1320 nt)	uhpC	membrane protein regulates uhpT expression
	?	CP009273	3841410 =	217 (0.830)					coding (575/1320 nt)	uhpC	membrane protein regulates uhpT expression

GGATCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/3841261‑3841409 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGT  >  CP009273/3841410‑3841555                                                                                                                                                      |||||||                                                                                                                                                   GGATCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATAC                                                                                                                                                          <  1:4339563/150‑1  GATCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACA                                                                                                                                                         >  1:3659644/1‑150  GATCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACA                                                                                                                                                         <  1:340419/150‑1    TCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAAC                                                                                                                                                       >  2:189162/1‑150    TCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAAC                                                                                                                                                       >  1:4122402/1‑150    TCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAAC                                                                                                                                                       >  1:2427945/1‑150    TCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAAC                                                                                                                                                       >  2:189120/1‑150     CTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACC                                                                                                                                                      >  1:1949505/1‑150      TCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCA                                                                                                                                                     >  2:2432073/1‑150      TCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCA                                                                                                                                                     <  1:4308185/150‑1      TCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCA                                                                                                                                                     >  1:4058989/1‑150      TCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCA                                                                                                                                                     >  1:3426033/1‑150      TCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCA                                                                                                                                                     >  1:3426040/1‑150      TCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCA                                                                                                                                                     >  1:3426044/1‑150      TCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCA                                                                                                                                                     >  1:3426066/1‑150       CTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAG                                                                                                                                                    <  1:4507230/150‑1       CTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAG                                                                                                                                                    >  2:119755/1‑150       CTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAG                                                                                                                                                    >  2:1943066/1‑150       CTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAG                                                                                                                                                    >  2:1943088/1‑150       CTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAG                                                                                                                                                    >  2:1943148/1‑150        TTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGC                                                                                                                                                   <  1:4376857/150‑1         TTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCA                                                                                                                                                  >  1:704459/1‑150          TACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAA                                                                                                                                                 >  1:330692/1‑150          TACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAA                                                                                                                                                 >  2:4143463/1‑150          TACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAA                                                                                                                                                 <  2:966100/150‑1          TACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTAGCGCCATACAACCAGCca                                                                                                                                                 >  1:3596011/1‑148                               CTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCA                                                                                                                            <  1:2565370/150‑1                               CTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCA                                                                                                                            <  2:549219/150‑1                                            AATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAAT                                                                                                               <  2:2214531/150‑1                                                                                   TAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGC                                                                        >  1:1287306/1‑150                                                                                                       GATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTG                                                    >  1:4250531/1‑150                                                                                                              TAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATA                                             >  1:1760034/1‑150                                                                                                                           AGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACC                                >  2:2895720/1‑150                                                                                                                           AGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACC                                >  2:9471/1‑150                                                                                                                                    CCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACACTGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTC                       <  1:2264849/150‑1                                                                                                                                    CCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTC                       <  1:2530648/150‑1                                                                                                                                    CCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTC                       <  1:2264853/150‑1                                                                                                                                    CCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTC                       <  2:4060439/150‑1                                                                                                                                    CCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTC                       <  1:2264850/150‑1                                                                                                                                             ATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGA              >  2:2952557/1‑150                                                                                                                                                GCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATA           >  1:2822244/1‑150                                                                                                                                                         CCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGT  <  1:305350/150‑1                                                                                                                                                      |||||||                                                                                                                                                   GGATCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/3841261‑3841409 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGT  >  CP009273/3841410‑3841555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.