Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,225,300 C→A 45.0% Q93K (CAG→AAG)  fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,225,3000CA45.0% 212.6 / inf 253Q93K (CAG→AAG) fadH2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base C (59/80);  new base A (47/67);  total (106/147)
Fisher's exact test for biased strand distribution p-value = 8.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

TTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGAC  >  CP009273/3225151‑3225449
                                                                                                                                                     |                                                                                                                                                     
tttATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATc                                                                                                                                                       >  1:4472494/1‑150 (MQ=255)
  tATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAg                                                                                                                                                     <  2:702554/150‑1 (MQ=255)
   aTGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAgg                                                                                                                                                    <  2:1364768/150‑1 (MQ=255)
    tGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGa                                                                                                                                                   <  2:1492799/150‑1 (MQ=255)
        gAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAggc                                                                                                                                               >  2:4153045/1‑150 (MQ=255)
        gAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAggc                                                                                                                                               <  2:575049/150‑1 (MQ=255)
           agcgcCCGTCAAGGCGGGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCTGCCAGATCCCACCCCATCGCACCAGTTCCGTAGCGGTACATCAGGAAggcggc                                                                                                                                            >  2:2510060/2‑150 (MQ=255)
            gcgcCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCa                                                                                                                                           <  2:2114547/150‑1 (MQ=255)
             cgcCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCaa                                                                                                                                          >  1:3553979/1‑150 (MQ=255)
             cgcCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCaa                                                                                                                                          >  1:1312427/1‑150 (MQ=255)
                ccGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAAt                                                                                                                                       <  2:1138004/150‑1 (MQ=255)
                ccGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAAt                                                                                                                                       <  1:4359204/150‑1 (MQ=255)
                   tCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGc                                                                                                                                    <  2:3967434/150‑1 (MQ=255)
                    cACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCACAATAGcc                                                                                                                                   >  2:2579319/1‑150 (MQ=255)
                    cACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGcc                                                                                                                                   >  2:617931/1‑150 (MQ=255)
                    cACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGcc                                                                                                                                   <  2:3305798/150‑1 (MQ=255)
                     aCGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGccc                                                                                                                                  >  2:4091910/1‑150 (MQ=255)
                     aCGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGccc                                                                                                                                  >  1:2483403/1‑150 (MQ=255)
                      cGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCt                                                                                                                                 >  1:129087/1‑150 (MQ=255)
                       ggCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTg                                                                                                                                <  1:1205234/150‑1 (MQ=255)
                       ggCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTg                                                                                                                                <  2:1170403/150‑1 (MQ=255)
                          gTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCaa                                                                                                                             >  1:3869998/1‑150 (MQ=255)
                          gTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCCCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCaa                                                                                                                             <  2:1038491/150‑1 (MQ=255)
                            ggCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCACTGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAAt                                                                                                                           <  2:2386588/150‑1 (MQ=255)
                              cgcTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATATCCCTGCAAAttt                                                                                                                         <  2:3495389/150‑1 (MQ=255)
                              cgcTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAAttt                                                                                                                         <  2:3394222/150‑1 (MQ=255)
                              cgcTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAAttt                                                                                                                         >  2:784155/1‑150 (MQ=255)
                              cgcTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAAttt                                                                                                                         <  1:2124851/150‑1 (MQ=255)
                               gcTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAAtttt                                                                                                                        <  1:2877731/150‑1 (MQ=255)
                               gcTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAAtttt                                                                                                                        <  1:1905661/150‑1 (MQ=255)
                                cTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTg                                                                                                                       >  2:2426577/1‑150 (MQ=255)
                                   aTTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCAt                                                                                                                    >  2:3069673/1‑150 (MQ=255)
                                    ttGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATa                                                                                                                   >  1:1111133/1‑150 (MQ=255)
                                           cggcggCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGgcg                                                                                                            <  2:4011648/150‑1 (MQ=255)
                                            ggcggcATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGgcgc                                                                                                           >  1:185391/1‑150 (MQ=255)
                                             gcggcATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCt                                                                                                          <  1:4522411/150‑1 (MQ=255)
                                             gcggcATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCt                                                                                                          <  1:1822828/150‑1 (MQ=255)
                                             gcggcATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCt                                                                                                          <  2:4238885/150‑1 (MQ=255)
                                             gcggcATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCt                                                                                                          <  2:4300362/150‑1 (MQ=255)
                                               ggcATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTAc                                                                                                        >  2:3205529/1‑150 (MQ=255)
                                                  aTTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGc                                                                                                     >  1:1758495/1‑150 (MQ=255)
                                                    tGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTa                                                                                                   >  1:230764/1‑150 (MQ=255)
                                                     gCACCAGATTTAACAGGCGTTGGCATGGAANGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTAc                                                                                                  >  1:4275741/1‑150 (MQ=255)
                                                      cACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTAcc                                                                                                 >  2:1732160/1‑150 (MQ=255)
                                                       aCCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCa                                                                                                >  2:2269841/1‑150 (MQ=255)
                                                       aCCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCa                                                                                                <  2:2850502/150‑1 (MQ=255)
                                                           gATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCg                                                                                            <  2:1486254/150‑1 (MQ=255)
                                                                  cAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGTTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTgg                                                                                     <  1:2610594/150‑1 (MQ=255)
                                                                         tGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGcccc                                                                              <  1:4040516/150‑1 (MQ=255)
                                                                          ggCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCg                                                                             <  2:3719128/150‑1 (MQ=255)
                                                                            cATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTc                                                                           >  1:3707123/1‑150 (MQ=255)
                                                                            cATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTc                                                                           <  2:4472424/150‑1 (MQ=255)
                                                                             aTGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTcc                                                                          <  2:2632648/150‑1 (MQ=255)
                                                                             aTGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTcc                                                                          <  1:667590/150‑1 (MQ=255)
                                                                             aTGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTcc                                                                          >  2:3487367/1‑150 (MQ=255)
                                                                                  aGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATACGGTAGGCTGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCAtt                                                                     <  2:100940/150‑1 (MQ=255)
                                                                                   ggTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTg                                                                    <  1:547885/150‑1 (MQ=255)
                                                                                      ggCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAg                                                                 >  1:4112578/1‑150 (MQ=255)
                                                                                      ggCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAg                                                                 >  2:3985591/1‑150 (MQ=255)
                                                                                           aaTGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGcccc                                                            <  1:3912505/150‑1 (MQ=255)
                                                                                           aaTGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGcccc                                                            >  2:2455541/1‑150 (MQ=255)
                                                                                            aTGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGccccc                                                           >  1:3826664/1‑150 (MQ=255)
                                                                                                tCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCa                                                       >  2:2242597/1‑150 (MQ=255)
                                                                                                  aaCGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAAc                                                     >  2:995128/1‑150 (MQ=255)
                                                                                                     gacgCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGt                                                  >  1:3008778/1‑150 (MQ=255)
                                                                                                     gacgCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGt                                                  >  2:2608922/1‑150 (MQ=255)
                                                                                                     gacgCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGt                                                  >  1:2176599/1‑150 (MQ=255)
                                                                                                      acgCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACCTCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGtt                                                 <  2:1596365/150‑1 (MQ=255)
                                                                                                      acgCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGtt                                                 <  1:3158975/150‑1 (MQ=255)
                                                                                                        gccagccaGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTc                                               <  1:1184229/150‑1 (MQ=255)
                                                                                                        gccagccaGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTc                                               >  2:2492839/1‑150 (MQ=255)
                                                                                                         ccagccagATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCg                                              >  1:2572618/1‑150 (MQ=255)
                                                                                                          cagccagATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGt                                             <  2:4633496/150‑1 (MQ=255)
                                                                                                           agccagATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTg                                            >  2:2334435/1‑150 (MQ=255)
                                                                                                           agccagATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTg                                            >  1:782266/1‑150 (MQ=255)
                                                                                                                 aTCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCAt                                      <  1:1207344/150‑1 (MQ=255)
                                                                                                                   cccACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGTAGGCCCCCATCAACCGTTTCGTGCCCCATGa                                    >  2:288322/1‑150 (MQ=255)
                                                                                                                    ccACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAg                                   <  2:957551/150‑1 (MQ=255)
                                                                                                                     cacaCCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGt                                  <  2:2744981/150‑1 (MQ=255)
                                                                                                                          cATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGc                             >  2:3991605/1‑150 (MQ=255)
                                                                                                                           aTCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGcc                            >  2:3836525/1‑150 (MQ=255)
                                                                                                                            tCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCa                           <  1:1047541/150‑1 (MQ=255)
                                                                                                                              gCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATg                         >  1:2395629/1‑150 (MQ=255)
                                                                                                                                aCCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGaa                       >  1:2810134/1‑150 (MQ=255)
                                                                                                                                 ccATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAag                      <  1:1027564/150‑1 (MQ=255)
                                                                                                                                 ccATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAag                      >  1:1384448/1‑150 (MQ=255)
                                                                                                                                     tACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGAt                  <  2:199395/150‑1 (MQ=255)
                                                                                                                                       ccGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATcc                <  1:1571317/150‑1 (MQ=255)
                                                                                                                                       ccGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATcc                >  1:685315/1‑150 (MQ=255)
                                                                                                                                        cGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCt               <  2:3117613/150‑1 (MQ=255)
                                                                                                                                         gAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTg              <  1:1750107/150‑1 (MQ=255)
                                                                                                                                         gAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAAACGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTg              <  1:4430571/150‑1 (MQ=255)
                                                                                                                                          aaGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGc             >  2:1872430/1‑150 (MQ=255)
                                                                                                                                          aaGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGc             <  2:3337548/150‑1 (MQ=255)
                                                                                                                                            gCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCaa           <  1:1088075/150‑1 (MQ=255)
                                                                                                                                              ggTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACt         >  2:2033578/1‑150 (MQ=255)
                                                                                                                                              ggTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACt         <  1:1981627/150‑1 (MQ=255)
                                                                                                                                              ggTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACt         >  1:4561517/1‑150 (MQ=255)
                                                                                                                                                 aCATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGAt      <  1:3501712/150‑1 (MQ=255)
                                                                                                                                                 aCATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGAt      <  2:443554/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGAC  >  CP009273/3225151‑3225449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑