Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,237,654 C→A 14.0% M149I (ATG→ATT)  uxaC ← uronate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,237,6540CA14.0% 949.4 / 87.6 283M149I (ATG→ATT) uxaCuronate isomerase
Reads supporting (aligned to +/- strand):  ref base C (110/133);  new base A (29/11);  total (139/144)
Fisher's exact test for biased strand distribution p-value = 1.87e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

TCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTAT  >  CP009273/3237505‑3237801
                                                                                                                                                     |                                                                                                                                                   
tCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTc                                                                                                                                                     >  1:3328511/1‑150 (MQ=255)
tCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTc                                                                                                                                                     >  1:4022762/1‑150 (MQ=255)
  gTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCAt                                                                                                                                                   >  2:423060/1‑150 (MQ=255)
  gTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCAt                                                                                                                                                   <  2:282617/150‑1 (MQ=255)
       aGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATctgct                                                                                                                                              >  1:1644275/1‑150 (MQ=255)
         gTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCtgctgc                                                                                                                                            >  2:788946/1‑150 (MQ=255)
             ccTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCatga                                                                                                                                        <  1:2396900/150‑1 (MQ=255)
             ccTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCatga                                                                                                                                        <  1:2865220/150‑1 (MQ=255)
              cTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCatgat                                                                                                                                       >  1:3490377/1‑150 (MQ=255)
               tGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCatgatg                                                                                                                                      <  1:916458/150‑1 (MQ=255)
               tGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCatgatg                                                                                                                                      <  2:2974740/150‑1 (MQ=255)
               tGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCatgatg                                                                                                                                      <  1:3233243/150‑1 (MQ=255)
               tGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCatgatg                                                                                                                                      >  2:3434337/1‑150 (MQ=255)
                  tCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcg                                                                                                                                   >  1:3282693/1‑150 (MQ=255)
                  tCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcg                                                                                                                                   <  2:77535/150‑1 (MQ=255)
                  tCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcg                                                                                                                                   <  2:4178269/150‑1 (MQ=255)
                    gATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcgcg                                                                                                                                 <  2:3386526/150‑1 (MQ=255)
                               cTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGt                                                                                                                      >  1:2426205/1‑150 (MQ=255)
                                tttGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGtt                                                                                                                     <  2:1644748/150‑1 (MQ=255)
                                 ttGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTa                                                                                                                    <  2:985794/150‑1 (MQ=255)
                                  tGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTAt                                                                                                                   >  1:2444245/1‑150 (MQ=255)
                                   gTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATc                                                                                                                  >  1:3180371/1‑150 (MQ=255)
                                       ggACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTgc                                                                                                              >  1:1827883/1‑150 (MQ=255)
                                        gACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTgcg                                                                                                             >  1:1613089/1‑150 (MQ=255)
                                        gACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTgcg                                                                                                             <  1:3112154/150‑1 (MQ=255)
                                        gACGCCAGCTCGGCAGCACTTTGAGGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTgcg                                                                                                             <  2:560691/150‑1 (MQ=255)
                                            ccAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCcag                                                                                                         >  2:3460767/1‑150 (MQ=255)
                                             cAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCcagc                                                                                                        >  1:593710/1‑150 (MQ=255)
                                              aGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCcagca                                                                                                       >  1:1726139/1‑150 (MQ=255)
                                                  cGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTc                                                                                                   <  1:4246610/150‑1 (MQ=255)
                                                  cGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTc                                                                                                   <  1:1792431/150‑1 (MQ=255)
                                                   ggCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCg                                                                                                  >  2:3793175/1‑150 (MQ=255)
                                                   ggCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCg                                                                                                  <  2:1738871/150‑1 (MQ=255)
                                                     cagcaCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGtt                                                                                                <  1:181449/150‑1 (MQ=255)
                                                     cagcaCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGtt                                                                                                >  1:4556948/1‑150 (MQ=255)
                                                       gcaCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTAc                                                                                              >  1:2803144/1‑150 (MQ=255)
                                                        caCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACa                                                                                             >  2:1882017/1‑150 (MQ=255)
                                                         aCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACAt                                                                                            <  1:1979651/150‑1 (MQ=255)
                                                             tGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGt                                                                                        >  1:3804681/1‑150 (MQ=255)
                                                                tGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTcc                                                                                     >  2:4414733/1‑150 (MQ=255)
                                                                 ggTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCa                                                                                    <  1:3061871/150‑1 (MQ=255)
                                                                  gTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAg                                                                                   <  2:1002663/150‑1 (MQ=255)
                                                                    gAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGAt                                                                                 >  1:2991285/1‑150 (MQ=255)
                                                                     aaaGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGAtt                                                                                >  1:1532499/1‑150 (MQ=255)
                                                                      aaGAGCCGTCTTTTTCGATATCTAAGTGATTCTCCACAGAATAGATCGGGTCATCGGATGTGCCGACCATTATCACCATCATCTGCTGCATGATGCCGCGCGCGGAGAAATTATCCTGCGCCAGAATTTCGTTACATAAGTACCAGAtat                                                                               >  2:1417926/1‑148 (MQ=255)
                                                                         agCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCAt                                                                            <  1:3684429/150‑1 (MQ=255)
                                                                           ccGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCg                                                                          <  1:1030812/150‑1 (MQ=255)
                                                                               cTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAg                                                                      >  2:3141136/1‑150 (MQ=255)
                                                                               cTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAg                                                                      <  2:4581601/150‑1 (MQ=255)
                                                                                tttGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGt                                                                     <  2:1296944/150‑1 (MQ=255)
                                                                                tttGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGt                                                                     >  2:3708561/1‑150 (MQ=255)
                                                                                 ttGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGtt                                                                    <  2:4289063/150‑1 (MQ=255)
                                                                                   ggCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGa                                                                  <  2:427126/150‑1 (MQ=255)
                                                                                    gCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGAc                                                                 >  1:1308299/1‑150 (MQ=255)
                                                                                    gCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGAc                                                                 <  1:2361060/150‑1 (MQ=255)
                                                                                    gCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCACTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGAc                                                                 <  1:4070816/150‑1 (MQ=255)
                                                                                     cGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACg                                                                <  2:1996012/150‑1 (MQ=255)
                                                                                      gATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACgg                                                               >  2:3453822/1‑150 (MQ=255)
                                                                                      gATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACgg                                                               >  2:2752118/1‑150 (MQ=255)
                                                                                       aTCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGga                                                              <  2:304767/150‑1 (MQ=255)
                                                                                       aTCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGga                                                              <  1:4406697/150‑1 (MQ=255)
                                                                                       aTCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGga                                                              <  2:2182350/150‑1 (MQ=255)
                                                                                       aTCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTAGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGga                                                              <  2:116484/150‑1 (MQ=255)
                                                                                        tctcTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGgag                                                             <  1:1245582/150‑1 (MQ=255)
                                                                                          tctGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGaa                                                           <  2:4540498/150‑1 (MQ=255)
                                                                                          tctGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGaa                                                           >  2:3300586/1‑150 (MQ=255)
                                                                                            tGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAg                                                         >  2:4308620/1‑150 (MQ=255)
                                                                                            tGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAg                                                         >  1:4223527/1‑150 (MQ=255)
                                                                                              cGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCa                                                       >  2:2262500/1‑150 (MQ=255)
                                                                                               gTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCaa                                                      >  1:287878/1‑150 (MQ=255)
                                                                                                tGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAAt                                                     >  2:1614130/1‑150 (MQ=255)
                                                                                                 gATGCTCCAGAGAATCGATCGGGTCATGGGTGGTGCCTACCATTTTCACGTTCATATGCTGCATGATGCCGCGCGCGGTGAATTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAAtt                                                    <  2:3351129/150‑1 (MQ=255)
                                                                                                 gATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAAtt                                                    <  2:3505448/150‑1 (MQ=255)
                                                                                                  aTGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCCGATTTCATCGGCAGTTGACGGAGAAAGCAAttt                                                   >  2:888236/1‑150 (MQ=255)
                                                                                                  aTGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAAttt                                                   <  1:4301141/150‑1 (MQ=255)
                                                                                                  aTGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAAttt                                                   <  1:4182306/150‑1 (MQ=255)
                                                                                                   tGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTg                                                  <  1:1187545/150‑1 (MQ=255)
                                                                                                       ccAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAg                                              <  2:245586/150‑1 (MQ=255)
                                                                                                          gagaATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGa                                           <  2:4000703/150‑1 (MQ=255)
                                                                                                            gaATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATa                                         <  1:2295590/150‑1 (MQ=255)
                                                                                                             aaTCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATAc                                        >  2:378269/1‑150 (MQ=255)
                                                                                                               tcgatcgGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTAATCGGCCGTTTACGGAGAAAGCAATTTGCCAGTGATACCa                                      >  2:4139715/1‑150 (MQ=255)
                                                                                                                cgatcgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCaa                                     >  2:1205199/1‑150 (MQ=255)
                                                                                                                cgatcgGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCaa                                     >  1:2976958/1‑150 (MQ=255)
                                                                                                                    cgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacgg                                 <  2:987817/150‑1 (MQ=255)
                                                                                                                      ggTCATCGTTTGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCTCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGACAGTGATACCAAacggac                               <  2:2071359/150‑1 (MQ=255)
                                                                                                                       gTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacggacg                              >  1:655467/1‑150 (MQ=255)
                                                                                                                        tCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacggacgg                             <  2:2631671/150‑1 (MQ=255)
                                                                                                                         cATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGgc                            >  2:2660048/1‑150 (MQ=255)
                                                                                                                          aTCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGgcg                           >  1:1672964/1‑150 (MQ=255)
                                                                                                                               tggtgCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGtt                      >  1:865214/1‑150 (MQ=255)
                                                                                                                                ggtgCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTc                     <  2:4619065/150‑1 (MQ=255)
                                                                                                                                 gtgCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCg                    <  1:2653187/150‑1 (MQ=255)
                                                                                                                                   gCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAg                  >  2:3511917/1‑150 (MQ=255)
                                                                                                                                   gCCGACCAATTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACAAAACGGACGGCGCAGTTCGAg                  >  2:3186364/1‑150 (MQ=255)
                                                                                                                                      gACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTg               >  1:1870104/1‑150 (MQ=255)
                                                                                                                                         cATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGt            >  2:3781328/1‑150 (MQ=255)
                                                                                                                                          aTTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTc           >  2:2343909/1‑150 (MQ=255)
                                                                                                                                             ttCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGCTACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAg        <  1:4623847/150‑1 (MQ=255)
                                                                                                                                              tCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGt       <  1:3730602/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTAT  >  CP009273/3237505‑3237801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑