Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,251,070 C→A 17.4% P100P (CCG→CCT)  yhaO ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,251,0700CA17.4% 758.9 / 95.3 248P100P (CCG→CCT) yhaOputative transporter
Reads supporting (aligned to +/- strand):  ref base C (107/97);  new base A (28/16);  total (135/113)
Fisher's exact test for biased strand distribution p-value = 1.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

CCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGGAGAAAAACAATCCCCG  >  CP009273/3250921‑3251218
                                                                                                                                                     |                                                                                                                                                    
 cTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCg                                                                                                                                                     <  1:1690773/150‑1 (MQ=255)
    ccGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                                                                  <  2:1817643/150‑1 (MQ=255)
      gTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTaa                                                                                                                                                >  2:3580363/1‑150 (MQ=255)
      gTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTaa                                                                                                                                                >  1:1553737/1‑150 (MQ=255)
       tCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAAt                                                                                                                                               <  2:23577/150‑1 (MQ=255)
         aCGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCt                                                                                                                                             >  1:3500427/1‑150 (MQ=255)
         aCGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCt                                                                                                                                             >  2:2003699/1‑150 (MQ=255)
          cGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCtt                                                                                                                                            <  1:1059914/150‑1 (MQ=255)
          cGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCtt                                                                                                                                            >  1:3181830/1‑150 (MQ=255)
          cGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCtt                                                                                                                                            >  1:1800713/1‑150 (MQ=255)
           gCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCttt                                                                                                                                           >  2:2827865/1‑150 (MQ=255)
             cGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTAATCTTTAc                                                                                                                                         >  1:127875/1‑150 (MQ=255)
               aaGGTATGCAGGTAGGAAGCGCTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTAATCTTTACAt                                                                                                                                       >  1:2395307/1‑150 (MQ=255)
               aaGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACAt                                                                                                                                       <  2:2114642/150‑1 (MQ=255)
                 ggTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTc                                                                                                                                     >  2:3674191/1‑150 (MQ=255)
                  gTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCt                                                                                                                                    >  2:3158178/1‑150 (MQ=255)
                     tGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGt                                                                                                                                 >  1:1415064/1‑150 (MQ=255)
                     tGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGt                                                                                                                                 <  1:3905470/150‑1 (MQ=255)
                      gCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTg                                                                                                                                >  2:2763052/1‑150 (MQ=255)
                        aGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGAt                                                                                                                              <  2:3208491/150‑1 (MQ=255)
                        aGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGAt                                                                                                                              <  2:3598004/150‑1 (MQ=255)
                           tAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTAATCTTTACATTCTGGTGATTCt                                                                                                                           <  2:61158/150‑1 (MQ=255)
                            aGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTg                                                                                                                          >  1:2296772/1‑150 (MQ=255)
                            aGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTg                                                                                                                          >  2:791162/1‑150 (MQ=255)
                             ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGc                                                                                                                         >  2:2480744/1‑150 (MQ=255)
                             ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGc                                                                                                                         >  1:2499577/1‑150 (MQ=255)
                             ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGc                                                                                                                         <  1:2376752/150‑1 (MQ=255)
                               aaGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCa                                                                                                                       <  1:264333/150‑1 (MQ=255)
                                 gCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGc                                                                                                                     >  1:2437760/1‑150 (MQ=255)
                                     tATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTAt                                                                                                                 >  2:2665498/1‑150 (MQ=255)
                                      aTCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTagt                                                                                                                >  2:3348830/1‑148 (MQ=255)
                                       tCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATta                                                                                                               <  2:38080/150‑1 (MQ=255)
                                       tCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATta                                                                                                               <  2:4342652/150‑1 (MQ=255)
                                         gTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATtaat                                                                                                             <  2:2662530/150‑1 (MQ=255)
                                           tGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATtaataa                                                                                                           >  2:2198592/1‑150 (MQ=255)
                                             gTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAAc                                                                                                         <  1:4245795/150‑1 (MQ=255)
                                               gATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACaa                                                                                                       <  2:2964528/150‑1 (MQ=255)
                                                aTGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAAt                                                                                                      <  2:2791353/150‑1 (MQ=255)
                                                  ggCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCg                                                                                                    <  1:3460920/150‑1 (MQ=255)
                                                    cGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCt                                                                                                  <  1:2796847/150‑1 (MQ=255)
                                                     ggTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTg                                                                                                 <  2:2622598/150‑1 (MQ=255)
                                                     ggTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGACCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTg                                                                                                 <  2:2297642/150‑1 (MQ=255)
                                                       tGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGaa                                                                                               >  1:2008081/1‑150 (MQ=255)
                                                             aGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGAt                                                                                         >  1:1747787/1‑150 (MQ=255)
                                                              gACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATa                                                                                        <  2:1184389/150‑1 (MQ=255)
                                                              gACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATa                                                                                        >  1:3238018/1‑150 (MQ=255)
                                                              gACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATa                                                                                        >  1:920392/1‑150 (MQ=255)
                                                               aCGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATAc                                                                                       <  1:1057718/150‑1 (MQ=255)
                                                               aCGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATAc                                                                                       <  1:4533927/150‑1 (MQ=255)
                                                               aCGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATAc                                                                                       <  1:2883354/150‑1 (MQ=255)
                                                                 gAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACAt                                                                                     <  1:1602915/150‑1 (MQ=255)
                                                                  aaCATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACAtt                                                                                    >  2:501401/1‑150 (MQ=255)
                                                                    cATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGc                                                                                  >  2:3955385/1‑150 (MQ=255)
                                                                     aTCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGcc                                                                                 >  1:2444228/1‑150 (MQ=255)
                                                                     aTCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGcc                                                                                 <  2:1778238/150‑1 (MQ=255)
                                                                     aTCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGcc                                                                                 >  2:3387912/1‑150 (MQ=255)
                                                                     aTCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGcc                                                                                 >  2:3122653/1‑150 (MQ=255)
                                                                        cAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCggg                                                                              >  1:3712296/1‑150 (MQ=255)
                                                                          aaTCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTa                                                                            >  2:1957471/1‑150 (MQ=255)
                                                                          aaTCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGACGGGTa                                                                            >  2:4570602/1‑150 (MQ=255)
                                                                          aaTCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACAGTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTa                                                                            <  2:2798334/150‑1 (MQ=255)
                                                                           aTCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTaa                                                                           <  1:1942755/150‑1 (MQ=255)
                                                                            tCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAAc                                                                          >  1:85075/1‑150 (MQ=255)
                                                                             cACCAGCCTTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTACTCTTTACATTCTGGTGATTCTGCCAGCGTATTAATCAACAATCGCTGAAACAGATACATTGCCGGGTAAcc                                                                         <  2:2899484/150‑1 (MQ=255)
                                                                             cACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAAcc                                                                         <  2:4069849/150‑1 (MQ=255)
                                                                               ccAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCaa                                                                       >  2:3974580/1‑150 (MQ=255)
                                                                                            aTAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTACCCAATCACCGATGagag                                                          >  2:4075801/1‑150 (MQ=255)
                                                                                             tAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGc                                                         <  1:4622130/150‑1 (MQ=255)
                                                                                               gagaGCACCTAAAAGTAACCACCATTTATTACCTAAATAACCGCTAAACACCATCGGGAAAACTTTACAAACAACAGAATCAGCCCGCGTAATAATAAACAAACGCATAAACACATACAATGCCGCGTAAACACACACCGAAGCGAGaaa                                                       >  2:3666093/1‑150 (MQ=255)
                                                                                                agCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCaaa                                                      >  2:308172/1‑150 (MQ=255)
                                                                                                agCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGAATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCaaa                                                      >  2:3256577/1‑150 (MQ=255)
                                                                                                 gcgcACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCaaaa                                                     <  2:865001/150‑1 (MQ=255)
                                                                                                    cACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATa                                                  >  1:4039823/1‑150 (MQ=255)
                                                                                                            aGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAAt                                          <  2:3740511/150‑1 (MQ=255)
                                                                                                              gATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATcc                                        >  1:3679389/1‑150 (MQ=255)
                                                                                                              gATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTAGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATcc                                        >  2:2671656/1‑150 (MQ=255)
                                                                                                                tGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCa                                      >  2:2039576/1‑150 (MQ=255)
                                                                                                                 gCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCAt                                     <  1:1114158/150‑1 (MQ=255)
                                                                                                                  ccccAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATc                                    >  1:3076957/1‑150 (MQ=255)
                                                                                                                   cccAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCa                                   >  1:3923655/1‑150 (MQ=255)
                                                                                                                   cccAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCa                                   >  1:3929680/1‑150 (MQ=255)
                                                                                                                   cccAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCa                                   <  1:1341127/150‑1 (MQ=255)
                                                                                                                    ccAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCaa                                  >  2:2470427/1‑150 (MQ=255)
                                                                                                                       gTTTTTACCTAAATAACCGCTAATGACGCTCGGGTCATCTTTACATTCTGGTGATTCTGCCAGCGTATGAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAAGCACCGATGTGAGCAAAAATACCCACAATCCCATCAAAcc                               >  1:1115433/1‑150 (MQ=255)
                                                                                                                         ttttACCTAAATAACCGCTAATGACGCTAGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGa                             <  1:2060968/150‑1 (MQ=255)
                                                                                                                            tACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATACACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCCCCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCt                          <  2:1233052/150‑1 (MQ=255)
                                                                                                                               cTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCa                       >  2:4190956/1‑150 (MQ=255)
                                                                                                                                 aaaTAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCAcc                     >  2:4626025/1‑150 (MQ=255)
                                                                                                                                  aaTAACCGCTAATGACGCTAGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCg                    >  1:1504622/1‑150 (MQ=255)
                                                                                                                                     aaCCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGga                 >  1:414342/1‑150 (MQ=255)
                                                                                                                                      aCCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGgag                <  1:3285229/150‑1 (MQ=255)
                                                                                                                                       ccGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGgaga               >  1:4371175/1‑150 (MQ=255)
                                                                                                                                        cGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGGAGaa              >  2:88622/1‑150 (MQ=255)
                                                                                                                                          cTAATGACGCTCGGGTAATCTTTACATTCTGTTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAAACGACCTGCACCGGGAGaaaa            >  1:3158201/1‑150 (MQ=255)
                                                                                                                                           tAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGGAGaaaaa           >  2:1292722/1‑150 (MQ=255)
                                                                                                                                            aaTGACGCTAGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGGAGAAAAAc          <  1:2347020/150‑1 (MQ=255)
                                                                                                                                             aTGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGGAGAAAAACa         >  1:768811/1‑150 (MQ=255)
                                                                                                                                              tGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGGAGAAAAACaa        >  2:1694751/1‑150 (MQ=255)
                                                                                                                                              tGACGCTAGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGGAGAAAAACaa        <  1:3580369/150‑1 (MQ=255)
                                                                                                                                                    tCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGGAGAAAAACAATCCCcg  <  2:2767324/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAATACCCACAATCCCATCAAACCGACCTGCACCGGGAGAAAAACAATCCCCG  >  CP009273/3250921‑3251218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑