Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,266,646 C→A 100% G91C (GGT→TGT)  garL ← alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,266,6460CA97.7% 877.1 / 10.0 259G91C (GGT→TGT) garLalpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (126/127);  total (129/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 259 total aligned reads displayed.

GAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGT  >  CP009273/3266500‑3266792
                                                                                                                                                  |                                                                                                                                                  
gAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGat                                                                                                                                                 >  2:3482119/1‑150 (MQ=255)
gAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGat                                                                                                                                                 >  2:3000113/1‑150 (MQ=255)
 aaaTAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGata                                                                                                                                                <  2:777733/150‑1 (MQ=255)
  aataatCCGCCACGGTGCCAAACATCTTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGatat                                                                                                                                               >  2:1570126/1‑150 (MQ=255)
  aataatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGatat                                                                                                                                               <  2:1103095/150‑1 (MQ=255)
    taatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATcc                                                                                                                                             <  1:2469617/150‑1 (MQ=255)
     aatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCa                                                                                                                                            <  1:2045744/150‑1 (MQ=255)
     aatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCa                                                                                                                                            <  2:4575442/150‑1 (MQ=255)
     aatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCa                                                                                                                                            >  2:256359/1‑150 (MQ=255)
      atCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCag                                                                                                                                           <  2:4294931/150‑1 (MQ=255)
          gCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCagaaga                                                                                                                                       >  1:1739225/1‑150 (MQ=255)
             aCGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGc                                                                                                                                    >  1:915761/1‑150 (MQ=255)
                 tGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTtaa                                                                                                                                <  1:718701/150‑1 (MQ=255)
                    cAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTtaataa                                                                                                                             >  1:4333979/1‑150 (MQ=255)
                     aaaCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTaataat                                                                                                                            >  1:3794577/1‑150 (MQ=255)
                     aaaCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTaataat                                                                                                                            >  2:1394163/1‑150 (MQ=255)
                     aaaCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTaataat                                                                                                                            <  2:3410427/150‑1 (MQ=255)
                       aCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTa                                                                                                                          <  1:4579614/150‑1 (MQ=255)
                       aCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTa                                                                                                                          >  2:3842740/1‑150 (MQ=255)
                       aCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTa                                                                                                                          <  2:2914663/150‑1 (MQ=255)
                        cATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTAc                                                                                                                         >  1:4442990/1‑150 (MQ=255)
                             tGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCt                                                                                                                    >  2:3490559/1‑150 (MQ=255)
                             tGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCt                                                                                                                    >  2:1243437/1‑150 (MQ=255)
                                 gcgGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGtt                                                                                                                <  1:1917302/150‑1 (MQ=255)
                                   ggTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTgg                                                                                                              >  1:924775/1‑150 (MQ=255)
                                   ggTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTgg                                                                                                              >  1:3829479/1‑150 (MQ=255)
                                   ggTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTgg                                                                                                              <  1:1340863/150‑1 (MQ=255)
                                      gagaAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCg                                                                                                           >  2:1198690/1‑150 (MQ=255)
                                             ggAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCg                                                                                                    >  1:333935/1‑150 (MQ=255)
                                             ggAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCg                                                                                                    >  1:404320/1‑150 (MQ=255)
                                             ggAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGAAATCCAGAAGACTCTTAATAATTACCGGCTCGTTGGTCGGCACTCg                                                                                                    >  2:675824/1‑150 (MQ=255)
                                              gagaCGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGc                                                                                                   >  2:1809954/1‑150 (MQ=255)
                                               agaCGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCa                                                                                                  >  1:1220736/1‑150 (MQ=255)
                                               agaCGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCa                                                                                                  >  1:4536491/1‑150 (MQ=255)
                                                  cgccgcGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCacta                                                                                               <  2:2252287/150‑1 (MQ=255)
                                                      gcgAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTgg                                                                                           <  2:4622376/150‑1 (MQ=255)
                                                       cgAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGgc                                                                                          <  1:215214/150‑1 (MQ=255)
                                                       cgAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGgc                                                                                          <  2:226510/150‑1 (MQ=255)
                                                       cgAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGgc                                                                                          <  2:2710118/150‑1 (MQ=255)
                                                        gAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGgcg                                                                                         >  2:2513495/1‑150 (MQ=255)
                                                        gAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGgcg                                                                                         >  1:1629389/1‑150 (MQ=255)
                                                            gCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgct                                                                                     >  1:3688980/1‑150 (MQ=255)
                                                             ccTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctg                                                                                    >  2:1733056/1‑150 (MQ=255)
                                                                  cGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctggcgct                                                                               <  1:106194/150‑1 (MQ=255)
                                                                  cGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctggcgct                                                                               <  2:4232804/150‑1 (MQ=255)
                                                                   ggTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctggcgctg                                                                              <  1:3501711/150‑1 (MQ=255)
                                                                   ggTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctggcgctg                                                                              >  1:2371154/1‑150 (MQ=255)
                                                                     tGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGcc                                                                            <  2:4342478/150‑1 (MQ=255)
                                                                        gTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCttt                                                                         <  2:812513/150‑1 (MQ=255)
                                                                           aCGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGATGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCaa                                                                      <  1:68596/150‑1 (MQ=255)
                                                                           aCGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCaa                                                                      >  2:2869284/1‑150 (MQ=255)
                                                                            cGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAg                                                                     <  1:334654/150‑1 (MQ=255)
                                                                             gggTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAgg                                                                    <  1:853136/150‑1 (MQ=255)
                                                                              ggTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGc                                                                   <  1:2182021/150‑1 (MQ=255)
                                                                               gTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGcc                                                                  <  1:3422398/150‑1 (MQ=255)
                                                                                ttGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCa                                                                 <  1:3608503/150‑1 (MQ=255)
                                                                                      ccACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACt                                                           <  2:264698/150‑1 (MQ=255)
                                                                                      ccACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACt                                                           <  1:11015/150‑1 (MQ=255)
                                                                                       cACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTg                                                          <  2:844733/150‑1 (MQ=255)
                                                                                        aCCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGc                                                         <  2:3199892/150‑1 (MQ=255)
                                                                                           gCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGa                                                      >  2:3919936/1‑150 (MQ=255)
                                                                                           gCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGa                                                      <  2:3207094/150‑1 (MQ=255)
                                                                                           gCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGa                                                      >  2:4495253/1‑150 (MQ=255)
                                                                                              aGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATa                                                   >  2:561918/1‑150 (MQ=255)
                                                                                               gCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATaa                                                  <  2:1641475/150‑1 (MQ=255)
                                                                                                 tctGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAAc                                                <  1:2112267/150‑1 (MQ=255)
                                                                                                  ctGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGNCTTTCAAGGCCATTAACTGCGGAATAAACg                                               <  1:4282973/150‑1 (MQ=255)
                                                                                                       tCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGgag                                          <  1:1251976/150‑1 (MQ=255)
                                                                                                        ccTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGgaga                                         >  1:3559005/1‑150 (MQ=255)
                                                                                                        ccTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGgaga                                         <  2:1177599/150‑1 (MQ=255)
                                                                                                         ctctTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGat                                        <  1:2771101/150‑1 (MQ=255)
                                                                                                          tctTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGata                                       <  2:1986797/150‑1 (MQ=255)
                                                                                                           ctTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGatat                                      >  2:3848317/1‑150 (MQ=255)
                                                                                                            ttttttttCTACAAAAGGAATCAGGAAGTTTTAGAAACAGATATCCAGAAGCAGCTTTATAATTACCGGCTCGTTGGTCTGCACTCTCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATc                                     <  1:2533998/150‑1 (MQ=255)
                                                                                                            ttttGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATc                                     >  2:3712228/1‑150 (MQ=255)
                                                                                                             tttGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCg                                    >  2:1904270/1‑150 (MQ=255)
                                                                                                               tGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGtt                                  <  1:2275123/150‑1 (MQ=255)
                                                                                                                 tttCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTg                                >  2:651481/1‑150 (MQ=255)
                                                                                                                  ttCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTgg                               <  1:2947665/150‑1 (MQ=255)
                                                                                                                  ttCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTgg                               >  1:499345/1‑150 (MQ=255)
                                                                                                                      aCAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCa                           <  2:4012978/150‑1 (MQ=255)
                                                                                                                       cAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCAt                          <  2:3365656/150‑1 (MQ=255)
                                                                                                                         aaaGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGt                        >  2:3570176/1‑150 (MQ=255)
                                                                                                                         aaaGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGt                        >  2:376109/1‑150 (MQ=255)
                                                                                                                          aaGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGtt                       <  1:2458941/150‑1 (MQ=255)
                                                                                                                          aaGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGtt                       >  1:2231310/1‑150 (MQ=255)
                                                                                                                           aGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTc                      <  2:973063/150‑1 (MQ=255)
                                                                                                                           aGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTc                      >  2:4131739/1‑150 (MQ=255)
                                                                                                                            ggAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCCAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCg                     <  1:760376/150‑1 (MQ=255)
                                                                                                                            ggAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCg                     >  1:276322/1‑150 (MQ=255)
                                                                                                                            ggAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCg                     <  1:1724202/150‑1 (MQ=255)
                                                                                                                                tCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCAt                 <  2:1740733/150‑1 (MQ=255)
                                                                                                                                  aGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATcc               >  1:2655646/1‑150 (MQ=255)
                                                                                                                                  aGGAAGTTATAGAAACAGATATCCAGAAGACGCTAAATAAAAACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTACGCCATcc               >  2:1251176/1‑150 (MQ=255)
                                                                                                                                     aaGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAATGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGc            >  1:149601/1‑150 (MQ=255)
                                                                                                                                     aaGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGc            >  1:3922508/1‑150 (MQ=255)
                                                                                                                                        ttATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAcc         >  1:644401/1‑150 (MQ=255)
                                                                                                                                         tataGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAcca        <  1:4007276/150‑1 (MQ=255)
                                                                                                                                          ataGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAccag       >  1:501210/1‑150 (MQ=255)
                                                                                                                                               aaCAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGt  >  2:2442595/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGT  >  CP009273/3266500‑3266792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑