Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,334,892 G→T 41.7% D90Y (GAT→TAT)  kdsD → D‑arabinose 5‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,334,8920GT41.7% 336.9 / inf 299D90Y (GAT→TAT) kdsDD‑arabinose 5‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (83/91);  new base T (54/70);  total (138/161)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 300 total aligned reads displayed.

TTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGGCGCG  >  CP009273/3334743‑3335040
                                                                                                                                                     |                                                                                                                                                    
ttGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTg                                                                                                                                                      >  1:4263942/1‑150 (MQ=255)
 tGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGa                                                                                                                                                     >  2:3752603/1‑150 (MQ=255)
 tGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGa                                                                                                                                                     >  2:2179012/1‑150 (MQ=255)
    cTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTAttt                                                                                                                                                  <  2:3799096/150‑1 (MQ=255)
    cTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGAttt                                                                                                                                                  <  1:2684674/150‑1 (MQ=255)
     tgtgAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTa                                                                                                                                                 <  1:428861/150‑1 (MQ=255)
     tgtgAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTa                                                                                                                                                 >  1:1929804/1‑150 (MQ=255)
      gtgAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAg                                                                                                                                                >  2:4050107/1‑150 (MQ=255)
       tgAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAgg                                                                                                                                               >  2:1503898/1‑150 (MQ=255)
         aaaaGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCa                                                                                                                                             <  2:3325842/150‑1 (MQ=255)
               tGTTCTGGTGTAAAGGGATAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTa                                                                                                                                       <  1:1994371/150‑1 (MQ=255)
               tGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTa                                                                                                                                       <  2:2407170/150‑1 (MQ=255)
                gTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTAc                                                                                                                                      >  1:2341170/1‑150 (MQ=255)
                   cTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTAcccc                                                                                                                                   <  2:677618/150‑1 (MQ=255)
                    tGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCTCATGGTTATTTAGGCATGGTTACCCca                                                                                                                                  >  1:1291434/1‑150 (MQ=255)
                      gtgtAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCcaaa                                                                                                                                >  2:3882888/1‑148 (MQ=255)
                       tgtAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAg                                                                                                                               >  2:4624595/1‑150 (MQ=255)
                       tgtAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAg                                                                                                                               >  2:2638162/1‑150 (MQ=255)
                         tAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGa                                                                                                                             >  2:3769810/1‑150 (MQ=255)
                          aaaGGGAAAGTTGTCGTCATGGGGATGGGTAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGAt                                                                                                                            >  1:3552401/1‑150 (MQ=255)
                          aaaGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGAt                                                                                                                            >  1:1658847/1‑150 (MQ=255)
                          aaaGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGAt                                                                                                                            <  2:3138197/150‑1 (MQ=255)
                                aaaGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATgtggtg                                                                                                                      >  2:118403/1‑150 (MQ=255)
                                aaaGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATgtggtg                                                                                                                      >  1:2457596/1‑150 (MQ=255)
                                  aGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGAt                                                                                                                    >  1:3429448/1‑150 (MQ=255)
                                   gTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGAtt                                                                                                                   <  1:1353051/150‑1 (MQ=255)
                                   gTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGAtt                                                                                                                   <  1:292143/150‑1 (MQ=255)
                                     tGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGc                                                                                                                 <  1:36524/150‑1 (MQ=255)
                                      gtcgtcATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCt                                                                                                                >  2:2532980/1‑150 (MQ=255)
                                      gtcgtcATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCt                                                                                                                <  2:1219556/150‑1 (MQ=255)
                                        cgtcATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTAt                                                                                                              <  2:2143718/150‑1 (MQ=255)
                                        cgtcATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTAt                                                                                                              <  2:335844/150‑1 (MQ=255)
                                          tcATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTAtct                                                                                                            >  1:3979948/1‑150 (MQ=255)
                                           cATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTAtctc                                                                                                           >  2:3846782/1‑150 (MQ=255)
                                            aTGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATctct                                                                                                          <  1:2056213/150‑1 (MQ=255)
                                              ggggATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTaa                                                                                                        >  2:1285801/1‑150 (MQ=255)
                                                ggATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAAct                                                                                                      >  1:2839602/1‑150 (MQ=255)
                                                 gATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAActc                                                                                                     <  2:139535/150‑1 (MQ=255)
                                                  aTGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAActct                                                                                                    <  1:3924311/150‑1 (MQ=255)
                                                    gggAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTgg                                                                                                  <  2:4410474/150‑1 (MQ=255)
                                                      gAAAATCGGGGCATATTGGGCGAAAAATGGCTGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCACACAGGATGTGGTGATTGCTATCTCTAACTCTGGTg                                                                                                <  2:1119057/150‑1 (MQ=255)
                                                      gAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTg                                                                                                <  2:3449584/150‑1 (MQ=255)
                                                       aaaaTCGGGGCATATTGGGCGAAATATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCAAAGGATGTGGTGATTTCTATCTCTAACTCTGGTGa                                                                                               <  1:1720828/150‑1 (MQ=255)
                                                         aaTCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAAt                                                                                             <  1:797/150‑1 (MQ=255)
                                                          aTCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACAGTTACACCTTAATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATc                                                                                            <  2:1317393/150‑1 (MQ=255)
                                                           tCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATcc                                                                                           <  2:2727724/150‑1 (MQ=255)
                                                                 cATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGaa                                                                                     >  2:4087931/1‑150 (MQ=255)
                                                                  atatTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGaaa                                                                                    >  1:17818/1‑150 (MQ=255)
                                                                    atTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATc                                                                                  >  1:60525/1‑150 (MQ=255)
                                                                      tGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCAc                                                                                <  1:1435662/150‑1 (MQ=255)
                                                                      tGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCAc                                                                                <  1:3755591/150‑1 (MQ=255)
                                                                        ggCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGACTCCAGCGAAATCACgg                                                                              >  2:2692569/1‑150 (MQ=255)
                                                                            aaaaaTGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCGATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCtt                                                                          >  1:1184847/1‑150 (MQ=255)
                                                                               aaTGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAAt                                                                       >  2:4433765/1‑150 (MQ=255)
                                                                                aTGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAAtt                                                                      <  2:1899095/150‑1 (MQ=255)
                                                                                  ggcggcAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTcc                                                                    <  2:1448970/150‑1 (MQ=255)
                                                                                   gcggcAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCa                                                                   <  1:3084447/150‑1 (MQ=255)
                                                                                     ggcAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGt                                                                 >  1:3539436/1‑150 (MQ=255)
                                                                                       cAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGc                                                               >  1:2139709/1‑150 (MQ=255)
                                                                                       cAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGc                                                               <  1:4290746/150‑1 (MQ=255)
                                                                                        aaCGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCt                                                              <  2:4202802/150‑1 (MQ=255)
                                                                                            tttGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAg                                                          <  2:4537227/150‑1 (MQ=255)
                                                                                            tttGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAg                                                          <  1:1101459/150‑1 (MQ=255)
                                                                                             ttGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGc                                                         <  2:1618338/150‑1 (MQ=255)
                                                                                                 cAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCt                                                     >  2:1139062/1‑150 (MQ=255)
                                                                                                     aCCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCAc                                                 >  2:1976742/1‑150 (MQ=255)
                                                                                                       cGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGt                                               <  1:4095896/150‑1 (MQ=255)
                                                                                                        ggTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTa                                              <  1:4202228/150‑1 (MQ=255)
                                                                                                         gTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTAc                                             >  2:101326/1‑150 (MQ=255)
                                                                                                         gTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTAc                                             >  2:2585098/1‑150 (MQ=255)
                                                                                                          tACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTAcc                                            <  1:2462708/150‑1 (MQ=255)
                                                                                                          tACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTAcc                                            <  1:1503898/150‑1 (MQ=255)
                                                                                                          tACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTAcc                                            >  1:322226/1‑150 (MQ=255)
                                                                                                           acacCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCg                                           <  2:390245/150‑1 (MQ=255)
                                                                                                                  cATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCt                                    >  1:490177/1‑150 (MQ=255)
                                                                                                                   aTTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTg                                   >  1:1877539/1‑150 (MQ=255)
                                                                                                                       ttCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATc                               >  2:3570462/1‑150 (MQ=255)
                                                                                                                       ttCGTCCATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATc                               >  2:1682481/1‑150 (MQ=255)
                                                                                                                       ttCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATc                               >  2:321254/1‑150 (MQ=255)
                                                                                                                           tCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCg                           <  1:1634769/150‑1 (MQ=255)
                                                                                                                            ccATCCTGGTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCgg                          >  1:699100/1‑150 (MQ=255)
                                                                                                                              aTCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTc                        <  1:2349149/150‑1 (MQ=255)
                                                                                                                               tCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCg                       <  2:915368/150‑1 (MQ=255)
                                                                                                                                ccTGGTGAAGCCGCGCATGGTTATTTAGGCAGGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGc                      >  2:2236530/1‑150 (MQ=255)
                                                                                                                                ccTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGc                      >  1:3705333/1‑150 (MQ=255)
                                                                                                                                 cTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGcc                     <  2:4160010/150‑1 (MQ=255)
                                                                                                                                   ggTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCg                   <  1:2574127/150‑1 (MQ=255)
                                                                                                                                    gTGAAGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCgg                  >  2:1663405/1‑150 (MQ=255)
                                                                                                                                     tGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGga                 <  1:4384381/150‑1 (MQ=255)
                                                                                                                                       aaGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGgaga               <  2:2866078/150‑1 (MQ=255)
                                                                                                                                        aGCCGCGCATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGagag              >  2:784632/1‑150 (MQ=255)
                                                                                                                                             cgcATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAgcagca         <  1:2255089/150‑1 (MQ=255)
                                                                                                                                               cATGGTTATTTAGGCATGGTTACCCCTCAGGATGTGGTGATTGCTATCTCTAACTCTTGTGAATCCATCGAAATCACGGCCTTAATTCCAGTGCTTAAGAGTCTTCACGTACCGTTAATCTGCATCACCGGTCTCCCGGAGAGCAGCATg       <  1:168134/150‑1 (MQ=255)
                                                                                                                                               cATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATg       >  1:3169371/1‑150 (MQ=255)
                                                                                                                                               cATGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATg       <  1:665688/150‑1 (MQ=255)
                                                                                                                                                aTGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATgg      <  2:2573125/150‑1 (MQ=255)
                                                                                                                                                 tGGTTATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGgc     <  2:4491758/150‑1 (MQ=255)
                                                                                                                                                 tGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGgc     <  2:2355974/150‑1 (MQ=255)
                                                                                                                                                 tGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGgc     <  2:4277916/150‑1 (MQ=255)
                                                                                                                                                    tGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGgcgcg  <  2:3087645/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCAACGTTTGCCAGCACCGGTACACCTTCATTTTTCGTCCATCCTGGTGAAGCCGCGCATGGTGATTTAGGCATGGTTACCCCACAGGATGTGGTGATTGCTATCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAATTCCAGTGCTTAAGCGTCTTCACGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGGCGCG  >  CP009273/3334743‑3335040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑