BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,369,534	C→A	5.1%	A436E (GCG→GAG)	dcuD →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,369,534	0	C	A	5.1%	1046.8 / 22.3	271	A436E (GCG→GAG)	dcuD	putative transporter
Reads supporting (aligned to +/- strand): ref base C (118/138); new base A (5/9); total (123/148)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 279 total aligned reads displayed.

AGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGC  >  CP009273/3369385‑3369683

aGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACCGc                                                                                                                                                       >  1:4046121/1‑150 (MQ=255)

aGCCGGACTACATGTACCAGCGGTAGTAATGATTATGCCGATGCATTTAGCCACCACGCAAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGACGATACGTCAGGAATTGCAGGCGTTTCGCCTTATGCGGTGGTGAAGCGGACAGc                                                                                                                                                       >  2:3757525/1‑150 (MQ=255)

 gCCTGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTATCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCg                                                                                                                                                      <  2:4079082/150‑1 (MQ=255)

   cGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGAt                                                                                                                                                    >  1:1537030/1‑150 (MQ=255)

      aCTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATccc                                                                                                                                                 <  2:3526724/150‑1 (MQ=255)

          cATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATg                                                                                                                                             <  1:4437232/150‑1 (MQ=255)

          cATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATg                                                                                                                                             <  2:3236154/150‑1 (MQ=255)

           aTGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATgg                                                                                                                                            >  1:178326/1‑150 (MQ=255)

           aTGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGAGATCCCCATgg                                                                                                                                            <  2:2035203/150‑1 (MQ=255)

             gTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCTCCGAATACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCa                                                                                                                                          >  2:833208/1‑150 (MQ=255)

                ccAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTc                                                                                                                                       >  1:3683702/1‑150 (MQ=255)

                 cAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCg                                                                                                                                      >  1:1233954/1‑150 (MQ=255)

                  aGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCgg                                                                                                                                     >  2:3739974/1‑150 (MQ=255)

                  aGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCgg                                                                                                                                     <  2:3454541/150‑1 (MQ=255)

                     ggTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGttt                                                                                                                                  >  1:3048302/1‑150 (MQ=255)

                     ggTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGttt                                                                                                                                  >  2:591692/1‑150 (MQ=255)

                      gTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTc                                                                                                                                 <  2:806624/150‑1 (MQ=255)

                      gTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGAGATCCCCATGGCAGTCGGTTTc                                                                                                                                 <  1:1731639/150‑1 (MQ=255)

                        tGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCgt                                                                                                                               <  2:200045/150‑1 (MQ=255)

                          tAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCgtgg                                                                                                                             >  1:1158017/1‑150 (MQ=255)

                           aaTGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCgtggt                                                                                                                            >  2:1460724/1‑150 (MQ=255)

                           aaTGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCgtggt                                                                                                                            >  2:898378/1‑150 (MQ=255)

                            aTGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCgtggtg                                                                                                                           <  2:1616285/150‑1 (MQ=255)

                             tGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGa                                                                                                                          <  2:76256/150‑1 (MQ=255)

                              gATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGaa                                                                                                                         <  2:1412123/150‑1 (MQ=255)

                              gATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGaa                                                                                                                         <  1:2264924/150‑1 (MQ=255)

                               aTTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAat                                                                                                                        <  1:2482294/150‑1 (MQ=255)

                                  aTGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATg                                                                                                                     <  2:1028232/150‑1 (MQ=255)

                                   tGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGa                                                                                                                    <  2:2182232/150‑1 (MQ=255)

                                    gCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGAGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGAt                                                                                                                   >  1:265539/1‑150 (MQ=255)

                                      cGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTg                                                                                                                 >  2:2192952/1‑150 (MQ=255)

                                       gATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGc                                                                                                                >  1:35497/1‑150 (MQ=255)

                                       gATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGc                                                                                                                >  1:2162933/1‑150 (MQ=255)

                                       gATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGc                                                                                                                >  2:4518661/1‑150 (MQ=255)

                                        aTGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGcc                                                                                                               >  2:4154842/1‑150 (MQ=255)

                                          gCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGAGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCcac                                                                                                             <  1:3916946/150‑1 (MQ=255)

                                           cATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCcaca                                                                                                            >  1:3965952/1‑150 (MQ=255)

                                            aTTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACaa                                                                                                           >  2:514041/1‑150 (MQ=255)

                                            aTTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACaa                                                                                                           >  2:2144021/1‑150 (MQ=255)

                                             ttttGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAAt                                                                                                          >  1:2201555/1‑150 (MQ=255)

                                             ttttGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAAt                                                                                                          <  1:3173555/150‑1 (MQ=255)

                                             ttttGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAAt                                                                                                          <  2:3998224/150‑1 (MQ=255)

                                             ttttGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAAt                                                                                                          <  2:2462585/150‑1 (MQ=255)

                                              tttGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATc                                                                                                         <  2:376819/150‑1 (MQ=255)

                                               ttGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCa                                                                                                        <  1:1884912/150‑1 (MQ=255)

                                                tGCCACGACGCTAGCGCGCGCGGTTTCGCCGAGTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACACTCAc                                                                                                       <  2:3589388/150‑1 (MQ=255)

                                                   cACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCt                                                                                                    >  1:2461675/1‑150 (MQ=255)

                                                     cgacgCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTAt                                                                                                  <  1:4301142/150‑1 (MQ=255)

                                                     cgacgCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTAt                                                                                                  <  1:1516592/150‑1 (MQ=255)

                                                      gacgCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTAtt                                                                                                 <  1:3459793/150‑1 (MQ=255)

                                                       acgCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTAttt                                                                                                <  2:2989878/150‑1 (MQ=255)

                                                       acgCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTAttt                                                                                                <  2:2990036/150‑1 (MQ=255)

                                                       acgCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTAttt                                                                                                <  2:2119513/150‑1 (MQ=255)

                                                        cgCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTAtttt                                                                                               <  1:1104456/150‑1 (MQ=255)

                                                        cgCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTAtttt                                                                                               >  1:776774/1‑150 (MQ=255)

                                                         gCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGGGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTtta                                                                                              <  2:4626101/150‑1 (MQ=255)

                                                         gCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTtta                                                                                              <  2:3121034/150‑1 (MQ=255)

                                                             gcgcgcgcGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTaa                                                                                          <  2:4362090/150‑1 (MQ=255)

                                                                cgcgcgGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTc                                                                                       <  1:625811/150‑1 (MQ=255)

                                                                     ggTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTaa                                                                                  <  1:4414917/150‑1 (MQ=255)

                                                                     ggTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTaa                                                                                  >  1:861116/1‑150 (MQ=255)

                                                                      gTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTaaa                                                                                 <  1:724338/150‑1 (MQ=255)

                                                                        ttCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTaaaaa                                                                               <  1:98134/150‑1 (MQ=255)

                                                                         tCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAg                                                                              <  1:1348916/150‑1 (MQ=255)

                                                                          cGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGa                                                                             <  2:1714630/150‑1 (MQ=255)

                                                                            ccGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACa                                                                           >  1:85350/1‑150 (MQ=255)

                                                                              gATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACaaa                                                                         <  2:2969549/150‑1 (MQ=255)

                                                                              gATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACaaa                                                                         >  1:2756964/1‑150 (MQ=255)

                                                                                  aCTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAg                                                                     <  2:3277661/150‑1 (MQ=255)

                                                                                   cTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAgg                                                                    <  1:4451074/150‑1 (MQ=255)

                                                                                   cTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAgg                                                                    <  1:567454/150‑1 (MQ=255)

                                                                                    tGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGTCAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAATCAGgc                                                                   <  1:1530790/150‑1 (MQ=255)

                                                                                     gCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGgcc                                                                  <  2:3410442/150‑1 (MQ=255)

                                                                                     gCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGgcc                                                                  <  1:1443309/150‑1 (MQ=255)

                                                                                     gCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGgcc                                                                  <  1:1694579/150‑1 (MQ=255)

                                                                                      cGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGgccg                                                                 <  1:236284/150‑1 (MQ=255)

                                                                                      cGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGgccg                                                                 <  1:2106905/150‑1 (MQ=255)

                                                                                           gtggtCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTgg                                                            <  1:1804084/150‑1 (MQ=255)

                                                                                              gtCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGAGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGcc                                                         >  1:4248843/1‑150 (MQ=255)

                                                                                                cGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTg                                                       >  2:1869302/1‑150 (MQ=255)

                                                                                                  ttACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGtt                                                     >  1:2572454/1‑150 (MQ=255)

                                                                                                   tACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGttt                                                    <  2:2325596/150‑1 (MQ=255)

                                                                                                     cGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTg                                                  <  2:4592215/150‑1 (MQ=255)

                                                                                                     cGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAAACACGCTATTTTATTAAGTCATAAAAAAGACAAAACAGGCCGCCTGGGCCTGATTTg                                                  >  2:3062938/1‑150 (MQ=255)

                                                                                                           gAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTAc                                            >  2:1265848/1‑150 (MQ=255)

                                                                                                            aaTTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACt                                           <  1:3977407/150‑1 (MQ=255)

                                                                                                              ttGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACACAACAGGCCGCCTGGGCCTGTTTTGTATTACTTc                                         <  2:4253322/150‑1 (MQ=255)

                                                                                                                          cGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAAt                             >  2:3888977/1‑150 (MQ=255)

                                                                                                                          cGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAAt                             >  1:1654591/1‑150 (MQ=255)

                                                                                                                            ccTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGc                           <  1:349166/150‑1 (MQ=255)

                                                                                                                             cTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGcc                          <  2:2846720/150‑1 (MQ=255)

                                                                                                                              ttttGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCg                         >  1:4604721/1‑150 (MQ=255)

                                                                                                                               tttGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCgg                        >  2:672463/1‑150 (MQ=255)

                                                                                                                                ttGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGt                       <  1:591692/150‑1 (MQ=255)

                                                                                                                                      tggtgAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGacca                 >  1:331837/1‑150 (MQ=255)

                                                                                                                                            aGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCgt           >  2:2599327/1‑150 (MQ=255)

                                                                                                                                            aGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCgt           <  2:679062/150‑1 (MQ=255)

                                                                                                                                              cGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCgtgg         <  2:366043/150‑1 (MQ=255)

                                                                                                                                                  cAGAGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGc     >  2:3952092/1‑150 (MQ=255)

                                                                                                                                                     cGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGc  >  1:2061314/1‑150 (MQ=255)

AGCCGGACTACATGTACCAGCGGTTGTAATGATTATGCCGATGCATTTTGCCACGACGCTAGCGCGCGCGGTTTCGCCGATTACTGCGGTGGTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGC  >  CP009273/3369385‑3369683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`