Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,440,444 G→T 100% S118S (TCC→TCA)  rplE ← 50S ribosomal subunit protein L5

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,440,4440GT95.4% 830.2 / 27.0 258S118S (TCC→TCA) rplE50S ribosomal subunit protein L5
Reads supporting (aligned to +/- strand):  ref base G (6/6);  new base T (133/113);  total (139/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 258 total aligned reads displayed.

CGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTG  >  CP009273/3440298‑3440589
                                                                                                                                                  |                                                                                                                                                 
 ggCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCCCGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACa                                                                                                                                               <  2:1007276/150‑1 (MQ=255)
 ggCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACa                                                                                                                                               <  1:4253033/150‑1 (MQ=255)
 ggCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACa                                                                                                                                               >  2:4033022/1‑150 (MQ=255)
 ggCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACa                                                                                                                                               <  2:4052934/150‑1 (MQ=255)
 ggCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACa                                                                                                                                               <  2:4270087/150‑1 (MQ=255)
  gCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAg                                                                                                                                              <  1:3702208/150‑1 (MQ=255)
    cttcttCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGc                                                                                                                                            <  1:2229838/150‑1 (MQ=255)
         tcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACgg                                                                                                                                       >  1:1091440/1‑150 (MQ=255)
            tCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAg                                                                                                                                    >  2:3225705/1‑150 (MQ=255)
             cAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGt                                                                                                                                   >  2:4414694/1‑150 (MQ=255)
             cAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGATACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGt                                                                                                                                   >  2:3669102/1‑150 (MQ=255)
              aGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTc                                                                                                                                  <  2:3864792/150‑1 (MQ=255)
              aGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTc                                                                                                                                  >  2:1505652/1‑150 (MQ=255)
                aTTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCAc                                                                                                                                >  1:3183370/1‑150 (MQ=255)
                  ttCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACgg                                                                                                                              >  1:769974/1‑150 (MQ=255)
                     gCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATa                                                                                                                           >  1:542283/1‑150 (MQ=255)
                     gCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATa                                                                                                                           >  2:1832596/1‑150 (MQ=255)
                      cAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATAc                                                                                                                          <  1:687553/150‑1 (MQ=255)
                       agtagtGGTAATAGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACACATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATaag                                                                                                                         >  1:257874/1‑148 (MQ=255)
                        gtagtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGa                                                                                                                        >  1:3600275/1‑150 (MQ=255)
                         tagtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAg                                                                                                                       >  2:4519033/1‑150 (MQ=255)
                            tggtaatggtaaTATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTa                                                                                                                    <  2:1968848/150‑1 (MQ=255)
                            tggtaatggtaaTATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTa                                                                                                                    <  2:2488902/150‑1 (MQ=255)
                            tggtaatggtaaTATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTa                                                                                                                    >  1:2467616/1‑150 (MQ=255)
                            tggtaatggtaaTATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTa                                                                                                                    >  1:1496348/1‑150 (MQ=255)
                              gtaatggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACa                                                                                                                  >  1:1187146/1‑150 (MQ=255)
                                 atggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACATGCCACGGAAGTCACGGATACGAGGTACAGCa                                                                                                               >  2:231529/1‑150 (MQ=255)
                                   ggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAAt                                                                                                             <  1:3068939/150‑1 (MQ=255)
                                    gtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATa                                                                                                            >  1:1352521/1‑150 (MQ=255)
                                     taatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAg                                                                                                           <  2:2064639/150‑1 (MQ=255)
                                     taatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAg                                                                                                           >  2:1228356/1‑150 (MQ=255)
                                         atCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGAt                                                                                                       >  2:3403916/1‑150 (MQ=255)
                                              aaCCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGgc                                                                                                  >  1:2291426/1‑150 (MQ=255)
                                                 cACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCt                                                                                               <  1:1327400/150‑1 (MQ=255)
                                                      aCGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAg                                                                                          <  2:1087532/150‑1 (MQ=255)
                                                       cgcgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGa                                                                                         <  1:3997668/150‑1 (MQ=255)
                                                       cgcgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGa                                                                                         <  1:847017/150‑1 (MQ=255)
                                                        gcgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGaa                                                                                        <  2:2140039/150‑1 (MQ=255)
                                                          ggTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACt                                                                                      >  2:1562366/1‑150 (MQ=255)
                                                           gTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTc                                                                                     >  2:133092/1‑150 (MQ=255)
                                                            tCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTcc                                                                                    <  2:3136000/150‑1 (MQ=255)
                                                            tCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTcc                                                                                    >  1:4484097/1‑150 (MQ=255)
                                                             cGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTccc                                                                                   <  1:4171540/150‑1 (MQ=255)
                                                             cGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTccc                                                                                   <  1:1305902/150‑1 (MQ=255)
                                                              gACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCca                                                                                  >  2:881095/1‑150 (MQ=255)
                                                                 tttATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACAt                                                                               >  2:3324260/1‑150 (MQ=255)
                                                                 tttATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACAt                                                                               <  2:4584111/150‑1 (MQ=255)
                                                                  ttATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATg                                                                              <  2:1937920/150‑1 (MQ=255)
                                                                  ttATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATg                                                                              <  2:1209109/150‑1 (MQ=255)
                                                                   tATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGc                                                                             <  1:2875290/150‑1 (MQ=255)
                                                                    aTCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCg                                                                            <  2:2905985/150‑1 (MQ=255)
                                                                    aTCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCg                                                                            >  1:1790589/1‑150 (MQ=255)
                                                                        tAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCg                                                                        <  2:2804488/150‑1 (MQ=255)
                                                                         aGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGc                                                                       >  1:3413653/1‑150 (MQ=255)
                                                                            cGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCAc                                                                    >  2:4320704/1‑150 (MQ=255)
                                                                            cGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCAc                                                                    <  2:990272/150‑1 (MQ=255)
                                                                             gATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACg                                                                   >  1:4343165/1‑150 (MQ=255)
                                                                               tttCTTGGAAGATGAACTGCTCACGGACACCCATGCAGTAGTTACAACGACCGACTAAAGACTTAGCTGACAGTCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCACAAAGAACTCCCACATGCGTTCGCCACGCa                                                                 >  2:2601566/1‑150 (MQ=255)
                                                                               tttCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCa                                                                 <  2:949009/150‑1 (MQ=255)
                                                                                  cTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCagag                                                              <  1:2792768/150‑1 (MQ=255)
                                                                                      gAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATCCGAGGTACAGCAATAGTGATCAGGCGCTCCATGAACTCCCACATGCGTTCGCCACGCAGAGTTag                                                          >  2:3558481/1‑149 (MQ=255)
                                                                                      gAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTAc                                                          >  2:1361702/1‑150 (MQ=255)
                                                                                        aGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACtt                                                        >  1:3651551/1‑150 (MQ=255)
                                                                                         gatgatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACttt                                                       >  1:1534226/1‑150 (MQ=255)
                                                                                         gatgatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACttt                                                       >  1:32621/1‑150 (MQ=255)
                                                                                          atgatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACATTa                                                      >  2:83451/1‑150 (MQ=255)
                                                                                                 gCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGa                                               >  1:3538356/1‑150 (MQ=255)
                                                                                                   tCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATc                                             >  2:2994660/1‑150 (MQ=255)
                                                                                                      cGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGa                                          >  1:3239185/1‑150 (MQ=255)
                                                                                                      cGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGa                                          <  1:1395386/150‑1 (MQ=255)
                                                                                                       ggACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGAt                                         >  1:2680812/1‑150 (MQ=255)
                                                                                                        gACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATa                                        <  2:616776/150‑1 (MQ=255)
                                                                                                           acCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGcc                                     <  2:3088693/150‑1 (MQ=255)
                                                                                                           acCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGcc                                     <  2:3274955/150‑1 (MQ=255)
                                                                                                             ccATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCt                                   <  1:621795/150‑1 (MQ=255)
                                                                                                                   tGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGa                             <  1:1654826/150‑1 (MQ=255)
                                                                                                                    gTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAt                            <  1:303649/150‑1 (MQ=255)
                                                                                                                     tAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAtt                           <  1:4066717/150‑1 (MQ=255)
                                                                                                                     tAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAtt                           <  1:2013820/150‑1 (MQ=255)
                                                                                                                      aGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAttt                          <  2:1735660/150‑1 (MQ=255)
                                                                                                                      aGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAttt                          <  2:2835442/150‑1 (MQ=255)
                                                                                                                          aCCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGaa                      <  2:2241945/150‑1 (MQ=255)
                                                                                                                            cACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGc                    >  1:4478674/1‑150 (MQ=255)
                                                                                                                              cGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCt                  <  2:1791375/150‑1 (MQ=255)
                                                                                                                              cGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCt                  <  2:542794/150‑1 (MQ=255)
                                                                                                                              cGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCt                  <  2:1150867/150‑1 (MQ=255)
                                                                                                                                aCCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGc                >  2:4199185/1‑150 (MQ=255)
                                                                                                                                aCCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGc                >  2:1427/1‑150 (MQ=255)
                                                                                                                                   gTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAAc             >  1:2900028/1‑150 (MQ=255)
                                                                                                                                     cGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAg           <  1:3352834/150‑1 (MQ=255)
                                                                                                                                     cGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAg           <  1:3751454/150‑1 (MQ=255)
                                                                                                                                       aaaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAt         >  1:91863/1‑150 (MQ=255)
                                                                                                                                       aaaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAt         >  1:882726/1‑150 (MQ=255)
                                                                                                                                       aaaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAt         >  1:1728770/1‑150 (MQ=255)
                                                                                                                                        aaGACTTATCTGACATGCCACTGAAGTCACGGATATGATGTACATCTATAGTTATCAGGCGCTCAATTAACTCACACATGCGTTAGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGAAGGATTTTGAAGCCTGCAACAGAtt        <  2:506504/150‑1 (MQ=255)
                                                                                                                                        aaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAtt        <  1:2588591/150‑1 (MQ=255)
                                                                                                                                         aGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAttt       <  1:2521552/150‑1 (MQ=255)
                                                                                                                                          gACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTg      <  2:827959/150‑1 (MQ=255)
                                                                                                                                           aCTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGc     >  1:2450937/1‑150 (MQ=255)
                                                                                                                                              tAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTg  >  2:4406794/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTG  >  CP009273/3440298‑3440589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑