Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,507,749 G→T 100% H224N (CAT→AAT)  rpe ← D‑ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,507,7490GT96.8% 860.1 / 16.0 265H224N (CAT→AAT) rpeD‑ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (7/2);  new base T (118/138);  total (125/140)
Fisher's exact test for biased strand distribution p-value = 8.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 266 total aligned reads displayed.

CAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTT  >  CP009273/3507603‑3507895
                                                                                                                                                  |                                                                                                                                                  
 aGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCCAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACtt                                                                                                                                                >  2:157033/1‑150 (MQ=255)
  ggTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTa                                                                                                                                               >  2:1003575/1‑150 (MQ=255)
     aataaCGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCt                                                                                                                                            >  2:2987098/1‑150 (MQ=255)
      ataaCGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCtt                                                                                                                                           >  1:4045128/1‑150 (MQ=255)
        aaCGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTg                                                                                                                                         >  1:4216440/1‑150 (MQ=255)
          cgcgTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGcc                                                                                                                                       <  1:1198769/150‑1 (MQ=255)
           gcgTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCa                                                                                                                                      <  2:2536149/150‑1 (MQ=255)
               tcttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTc                                                                                                                                  >  2:223278/1‑150 (MQ=255)
                cttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCa                                                                                                                                 >  2:680090/1‑150 (MQ=255)
                cttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCa                                                                                                                                 >  1:1615035/1‑150 (MQ=255)
                  tcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGTGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACt                                                                                                                               <  1:3689882/150‑1 (MQ=255)
                  tcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACt                                                                                                                               >  1:2282037/1‑150 (MQ=255)
                   cACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTg                                                                                                                              <  2:3887625/150‑1 (MQ=255)
                     ccTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTgcg                                                                                                                            >  2:1900964/1‑150 (MQ=255)
                       tGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCa                                                                                                                          >  1:2732667/1‑150 (MQ=255)
                        gCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAt                                                                                                                         <  1:2199503/150‑1 (MQ=255)
                        gCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAt                                                                                                                         <  2:1327463/150‑1 (MQ=255)
                         cGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAtt                                                                                                                        >  1:2838839/1‑150 (MQ=255)
                          gACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAttt                                                                                                                       <  1:9425/150‑1 (MQ=255)
                          gACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAttt                                                                                                                       >  1:2776290/1‑150 (MQ=255)
                              gccAACTCAAGCGCATACAGAGACATATCTACAGAAGCAGCAAGACCAGGAGCACTGTCTACCATCGAACCATAAAGATCAAAAGCGACGCCGCGAATAACATCAAACTTATTCATTACTTACCTTTGCCAGTACAATGCGCATTtcata                                                                                                                   >  2:2022548/3‑149 (MQ=255)
                                 aaCTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAAt                                                                                                                >  2:3696548/1‑150 (MQ=255)
                                      cAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACtt                                                                                                           <  1:3183006/150‑1 (MQ=255)
                                          gcATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACtttttt                                                                                                       <  1:188282/150‑1 (MQ=255)
                                                aGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTc                                                                                                 <  2:1963791/150‑1 (MQ=255)
                                                 gcgcCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTct                                                                                                <  2:1561482/150‑1 (MQ=255)
                                                   gcCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctgg                                                                                              <  1:4548859/150‑1 (MQ=255)
                                                   gcCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctgg                                                                                              <  1:3432081/150‑1 (MQ=255)
                                                   gcCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctgg                                                                                              <  2:1696977/150‑1 (MQ=255)
                                                   gcCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctgg                                                                                              >  1:1887611/1‑150 (MQ=255)
                                                    ccATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggc                                                                                             <  2:4185460/150‑1 (MQ=255)
                                                    ccATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggc                                                                                             <  2:293571/150‑1 (MQ=255)
                                                     cATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggct                                                                                            <  2:2286400/150‑1 (MQ=255)
                                                      atatCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggctg                                                                                           >  1:3500287/1‑150 (MQ=255)
                                                      atatCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggctg                                                                                           >  1:2691001/1‑150 (MQ=255)
                                                        atCGACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGt                                                                                         <  1:4485638/150‑1 (MQ=255)
                                                         tCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTc                                                                                        <  1:4596581/150‑1 (MQ=255)
                                                              cGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGa                                                                                   >  1:1144520/1‑150 (MQ=255)
                                                               gcagcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGAt                                                                                  >  1:3076947/1‑150 (MQ=255)
                                                               gcagcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGAt                                                                                  <  1:1262267/150‑1 (MQ=255)
                                                                cagcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGAtt                                                                                 <  1:3462092/150‑1 (MQ=255)
                                                                cagcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGAtt                                                                                 <  1:593413/150‑1 (MQ=255)
                                                                 agcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTg                                                                                >  2:4542637/1‑150 (MQ=255)
                                                                 agcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTg                                                                                <  2:2870545/150‑1 (MQ=255)
                                                                    agcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCg                                                                             >  2:2389709/1‑150 (MQ=255)
                                                                     gcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGa                                                                            <  1:877161/150‑1 (MQ=255)
                                                                      caAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGaa                                                                           <  1:1669704/150‑1 (MQ=255)
                                                                      caAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTGTTTGTAGTCTGGCTGGTCGAAGATTGCCGaa                                                                           >  2:1513681/1‑150 (MQ=255)
                                                                       aaGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCAGCAATGACTTGTTTGTAGTCTGGCTGGTCGAAGATTGCCGAAc                                                                          >  1:3972094/1‑150 (MQ=255)
                                                                            cAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcg                                                                     <  1:313052/150‑1 (MQ=255)
                                                                             aGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCGTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcga                                                                    >  2:1024894/1‑150 (MQ=255)
                                                                             aGGAGCACTGTCGACCAGCGTAACATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcga                                                                    <  2:2583089/150‑1 (MQ=255)
                                                                              ggAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcgac                                                                   <  2:3203142/150‑1 (MQ=255)
                                                                                aGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcgacga                                                                 >  1:1563286/1‑150 (MQ=255)
                                                                                  cACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGAGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAAc                                                               >  1:3205803/1‑150 (MQ=255)
                                                                                   aCTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACa                                                              >  1:1859624/1‑150 (MQ=255)
                                                                                    cTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACat                                                             <  2:2203582/150‑1 (MQ=255)
                                                                                         gACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCcg                                                        <  2:662432/150‑1 (MQ=255)
                                                                                            cAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCgcgc                                                     <  2:4346103/150‑1 (MQ=255)
                                                                                             atcGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcc                                                    >  2:424084/3‑150 (MQ=255)
                                                                                             aGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTGGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcc                                                    <  2:1096661/150‑1 (MQ=255)
                                                                                             aGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcc                                                    >  1:2595960/1‑150 (MQ=255)
                                                                                              gCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGTCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgccc                                                   <  2:4450702/150‑1 (MQ=255)
                                                                                               cGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccg                                                  >  2:3348022/1‑150 (MQ=255)
                                                                                               cGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccg                                                  >  2:1263327/1‑150 (MQ=255)
                                                                                                gTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccgc                                                 >  2:3568342/1‑150 (MQ=255)
                                                                                                 tACCCTCATGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTTCTTACCTTTGCCAGTTCACTGCTGATTTCATCAATGTCTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccgcc                                                <  2:3935457/150‑1 (MQ=255)
                                                                                                 tACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccgcc                                                <  2:4529682/150‑1 (MQ=255)
                                                                                                    cATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCa                                             >  1:3351092/1‑150 (MQ=255)
                                                                                                       cAAGATCAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCg                                          <  1:4427772/150‑1 (MQ=255)
                                                                                                       cAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCg                                          <  1:3875643/150‑1 (MQ=255)
                                                                                                        aaGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGa                                         >  2:3490464/1‑150 (MQ=255)
                                                                                                             cAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCg                                    >  1:2415674/1‑150 (MQ=255)
                                                                                                             cAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCg                                    <  2:1770000/150‑1 (MQ=255)
                                                                                                               aaaGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGcc                                  >  2:1777015/1‑150 (MQ=255)
                                                                                                                aaGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCa                                 >  1:2087867/1‑150 (MQ=255)
                                                                                                                    gACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtgt                             <  2:4144344/150‑1 (MQ=255)
                                                                                                                    gACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtgt                             <  1:2245872/150‑1 (MQ=255)
                                                                                                                     aCGCCGCGAATATCTTCAAACTTTTTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGTTCGAAGATTGCCGATCCGGAGACGAACATATACGCGCCCGCCGCAGCGATTTCGACAAtgtt                            <  1:728013/150‑1 (MQ=255)
                                                                                                                         cgcgAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTc                        <  2:30516/150‑1 (MQ=255)
                                                                                                                         cgcgAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTc                        <  2:471276/150‑1 (MQ=255)
                                                                                                                          gcgAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCa                       >  2:3143515/1‑150 (MQ=255)
                                                                                                                            gAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCAcc                     <  1:1596197/150‑1 (MQ=255)
                                                                                                                            gAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCAcc                     >  2:396070/1‑150 (MQ=255)
                                                                                                                             aaTATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCt                    <  1:3595736/150‑1 (MQ=255)
                                                                                                                              atatCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCtt                   >  1:1751697/1‑150 (MQ=255)
                                                                                                                              atatCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCtt                   <  1:4369903/150‑1 (MQ=255)
                                                                                                                                atCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCa                 >  1:3825575/1‑150 (MQ=255)
                                                                                                                                atCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCa                 <  2:4084639/150‑1 (MQ=255)
                                                                                                                                 tCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCAc                <  1:3022826/150‑1 (MQ=255)
                                                                                                                                    tCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGcc             >  2:1604054/1‑150 (MQ=255)
                                                                                                                                     cAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCa            <  1:1013254/150‑1 (MQ=255)
                                                                                                                                     cAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCa            <  2:780643/150‑1 (MQ=255)
                                                                                                                                     cAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCa            >  2:2069313/1‑150 (MQ=255)
                                                                                                                                       aaCTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCAcc          <  2:1078262/150‑1 (MQ=255)
                                                                                                                                        aCTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCg         <  2:3826360/150‑1 (MQ=255)
                                                                                                                                             ttCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCAc    >  1:1796681/1‑150 (MQ=255)
                                                                                                                                             ttCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCAc    >  1:4122958/1‑150 (MQ=255)
                                                                                                                                              tCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACt   >  2:3632737/1‑150 (MQ=255)
                                                                                                                                              tCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACt   <  1:3509447/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTT  >  CP009273/3507603‑3507895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑