Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,568,708 C→A 12.2% S93* (TCA→TAA)  yhgN → UPF0056 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,568,7080CA12.2% 914.1 / 69.4 261S93* (TCA→TAA) yhgNUPF0056 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (116/113);  new base A (17/15);  total (133/128)
Fisher's exact test for biased strand distribution p-value = 8.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 261 total aligned reads displayed.

TGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGCTCTG  >  CP009273/3568561‑3568856
                                                                                                                                                   |                                                                                                                                                    
tGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATT‑AAATGATTTTCCCCAGCGCTTCAgg                                                                                                                                                   <  2:2224202/150‑1 (MQ=255)
 gCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAgg                                                                                                                                                   >  1:269918/1‑150 (MQ=255)
 gCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAgg                                                                                                                                                   >  2:1156558/1‑150 (MQ=255)
        gCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATagc                                                                                                                                            <  1:1900120/150‑1 (MQ=255)
         ctctCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATagca                                                                                                                                           <  1:3610560/150‑1 (MQ=255)
           ctcCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATagcagc                                                                                                                                         <  1:3230775/150‑1 (MQ=255)
             ccTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCgg                                                                                                                                       <  2:3319383/150‑1 (MQ=255)
                 gTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCtt                                                                                                                                   <  2:1357684/150‑1 (MQ=255)
                 gTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCtt                                                                                                                                   >  2:1802280/1‑150 (MQ=255)
                  tGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTc                                                                                                                                  >  1:47944/1‑150 (MQ=255)
                  tGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTc                                                                                                                                  <  2:2539605/150‑1 (MQ=255)
                  tGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTc                                                                                                                                  >  2:1290995/1‑150 (MQ=255)
                   gATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTcc                                                                                                                                 <  1:1924007/150‑1 (MQ=255)
                    aTGCTGGTGTTCCTGTTTGCGGGTGAGACAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCg                                                                                                                                <  2:256127/150‑1 (MQ=255)
                    aTGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCg                                                                                                                                >  2:2338070/1‑150 (MQ=255)
                      gCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGc                                                                                                                              >  2:157671/1‑150 (MQ=255)
                      gCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGc                                                                                                                              <  1:2408333/150‑1 (MQ=255)
                       cTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCa                                                                                                                             >  1:1617441/1‑150 (MQ=255)
                            gtTCCTGTTTGCTGGTGAGAAAATTCTGGCTTTTCTTAGCCTTCGAGCAGAAACCGTCTCCATTTCTGTCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGa                                                                                                                        <  1:2001894/150‑1 (MQ=255)
                                   tttGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCa                                                                                                                 <  2:1248502/150‑1 (MQ=255)
                                   tttGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCa                                                                                                                 <  2:488928/150‑1 (MQ=255)
                                    ttGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCAt                                                                                                                <  1:1939061/150‑1 (MQ=255)
                                       cGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTa                                                                                                             <  2:4291333/150‑1 (MQ=255)
                                        gggTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTAt                                                                                                            <  2:888537/150‑1 (MQ=255)
                                          gTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCg                                                                                                          <  1:4419797/150‑1 (MQ=255)
                                            gagaAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTg                                                                                                        <  2:397605/150‑1 (MQ=255)
                                                 aaTTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGtt                                                                                                   >  2:928088/1‑150 (MQ=255)
                                                  aTTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTg                                                                                                  <  2:343259/150‑1 (MQ=255)
                                                    tCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGc                                                                                                >  1:247383/1‑150 (MQ=255)
                                                           tttCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCg                                                                                         >  2:725918/1‑150 (MQ=255)
                                                             tCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGtt                                                                                       <  2:2994828/150‑1 (MQ=255)
                                                                 aGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTc                                                                                   <  1:1441169/150‑1 (MQ=255)
                                                                     tACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCg                                                                               >  1:100538/1‑150 (MQ=255)
                                                                        gAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGc                                                                            >  1:4258127/1‑150 (MQ=255)
                                                                            aGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGAc                                                                        >  2:4407765/1‑150 (MQ=255)
                                                                              aaaCCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTa                                                                      >  1:3304731/1‑150 (MQ=255)
                                                                               aaCCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTAt                                                                     >  2:86767/1‑150 (MQ=255)
                                                                                aCCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTAtt                                                                    <  1:2559114/150‑1 (MQ=255)
                                                                                 ccGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtc                                                                   <  1:1475739/150‑1 (MQ=255)
                                                                                  cGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtct                                                                  >  2:750790/1‑150 (MQ=255)
                                                                                   gTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtctc                                                                 <  2:1516196/150‑1 (MQ=255)
                                                                                   gTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtctc                                                                 >  1:1845819/1‑150 (MQ=255)
                                                                                   gTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtctc                                                                 >  2:2905605/1‑150 (MQ=255)
                                                                                   gTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtctc                                                                 >  1:564326/1‑150 (MQ=255)
                                                                                   gTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATtctc                                                                 <  2:3774313/150‑1 (MQ=255)
                                                                                    tctcCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCg                                                                <  1:3056910/150‑1 (MQ=255)
                                                                                    tctcCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCg                                                                >  1:506361/1‑150 (MQ=255)
                                                                                     ctcCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGc                                                               >  1:3691185/1‑150 (MQ=255)
                                                                                     ctcCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGc                                                               >  1:3040083/1‑150 (MQ=255)
                                                                                      tcCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGcc                                                              <  2:4519326/150‑1 (MQ=255)
                                                                                       ccATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCa                                                             >  1:1153794/1‑150 (MQ=255)
                                                                                         aTTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACg                                                           >  2:2503187/1‑150 (MQ=255)
                                                                                           ttCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCt                                                         <  1:2332061/150‑1 (MQ=255)
                                                                                            tCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTg                                                        <  1:4630206/150‑1 (MQ=255)
                                                                                            tCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTg                                                        <  1:3553047/150‑1 (MQ=255)
                                                                                               ggcggcATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtg                                                     <  2:3346708/150‑1 (MQ=255)
                                                                                               ggcggcATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtg                                                     >  2:2790215/1‑150 (MQ=255)
                                                                                                gcggcATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtgt                                                    <  2:785445/150‑1 (MQ=255)
                                                                                                gcggcATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtgt                                                    <  1:2194636/150‑1 (MQ=255)
                                                                                                   gcATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtgttgt                                                 >  2:3784357/1‑150 (MQ=255)
                                                                                                   gcATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtgttgt                                                 <  2:68241/150‑1 (MQ=255)
                                                                                                    catcatTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGAtgttgtt                                                <  1:1300299/150‑1 (MQ=255)
                                                                                                       catTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGtc                                             <  1:4017120/150‑1 (MQ=255)
                                                                                                          tCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCtca                                          >  1:2194831/1‑150 (MQ=255)
                                                                                                          tCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCtca                                          <  2:1077981/150‑1 (MQ=255)
                                                                                                           cTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCtcat                                         <  2:4033850/150‑1 (MQ=255)
                                                                                                           cTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCtcat                                         >  1:1833883/1‑150 (MQ=255)
                                                                                                             gTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCtcatca                                       <  1:2491712/150‑1 (MQ=255)
                                                                                                             gTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCtcatca                                       >  1:805631/1‑150 (MQ=255)
                                                                                                              tttCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAg                                      >  2:2658094/1‑150 (MQ=255)
                                                                                                                tCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTa                                    >  1:3800207/1‑150 (MQ=255)
                                                                                                                 cTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTAc                                   >  2:614470/1‑150 (MQ=255)
                                                                                                                  tGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTAcc                                  >  1:3508163/1‑150 (MQ=255)
                                                                                                                   gATCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTAccc                                 >  1:862551/1‑150 (MQ=255)
                                                                                                                    aTCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCg                                >  2:3521996/1‑150 (MQ=255)
                                                                                                                     tCGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGa                               <  2:3158033/150‑1 (MQ=255)
                                                                                                                      cGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGaa                              <  2:1569539/150‑1 (MQ=255)
                                                                                                                      cGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGaa                              >  2:3592425/1‑150 (MQ=255)
                                                                                                                      cGCCATTAAAATGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGaa                              >  1:208981/1‑150 (MQ=255)
                                                                                                                       gCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAAt                             >  1:4154456/1‑150 (MQ=255)
                                                                                                                        ccATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATc                            <  2:125817/150‑1 (MQ=255)
                                                                                                                           ttAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGa                         >  2:2431840/1‑150 (MQ=255)
                                                                                                                             aaaaTGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATg                       <  1:3324748/150‑1 (MQ=255)
                                                                                                                             aaaaTGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATg                       <  2:2024938/150‑1 (MQ=255)
                                                                                                                             aaaaTGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATg                       <  2:543640/150‑1 (MQ=255)
                                                                                                                              aaaTGATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATgg                      >  2:630932/1‑150 (MQ=255)
                                                                                                                               aaTGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATggg                     <  2:4159181/150‑1 (MQ=255)
                                                                                                                                aTGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATgggg                    >  2:1461855/1‑150 (MQ=255)
                                                                                                                                  gATTTTCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCa                  <  2:1687245/150‑1 (MQ=255)
                                                                                                                                       tCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTgg             <  1:66409/150‑1 (MQ=255)
                                                                                                                                       tCCCCAGCGCTTAAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTgg             >  1:3856833/1‑150 (MQ=255)
                                                                                                                                         cccAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTg           <  1:3356582/150‑1 (MQ=255)
                                                                                                                                          ccAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGa          >  2:4085899/1‑150 (MQ=255)
                                                                                                                                            aGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGAtt        <  1:2531838/150‑1 (MQ=255)
                                                                                                                                              cgcTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGc      >  1:3460588/1‑150 (MQ=255)
                                                                                                                                                cTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGctc    >  1:1576735/1‑150 (MQ=255)
                                                                                                                                                cTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGctc    <  2:3245329/150‑1 (MQ=255)
                                                                                                                                                  tCAGGAAATAGGAGCGGGCTTCCGGCAGGTGTAGAGCCTTTTATAGTGCCGTTGGCAATTCAGTTCTTGGCCGGGCCGACTATTCTCGCCAAGCTGATGTTTTTGTCTCATCAGTACCCTAATCAGATGGGGAATCTGGTGATTTCTctg  <  1:4361877/150‑1 (MQ=255)
                                                                                                                                                  tCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGCTctg  <  1:28631/150‑1 (MQ=255)
                                                                                                                                                  tCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGCTctg  >  2:3861203/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAGCAGCGGGCTTCCGGCAGGTGAAGAGCCATTTATCGTGCCGTTGGCAATTCCGTTAGTCGCCGGGCCGACTATTCTCGCCACGCTGATGTTGTTGTCTCATCAGTACCCGAATCAGATGGGGCATCTGGTGATTGCTCTG  >  CP009273/3568561‑3568856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑