Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,646,984 G→T 6.5% pseudogene (394/483 nt) yhiS → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,646,9840GT 6.5% 1061.6 / 28.2 281pseudogene (394/483 nt)yhiSpseudogene
Reads supporting (aligned to +/- strand):  ref base G (101/160);  new base T (7/13);  total (108/173)
Fisher's exact test for biased strand distribution p-value = 8.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

AAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTACTTTTAAAAC  >  CP009273/3646835‑3647133
                                                                                                                                                     |                                                                                                                                                     
    tAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGaaaa                                                                                                                                                   >  2:1184999/1‑150 (MQ=255)
    tAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGaaaa                                                                                                                                                   >  2:2188582/1‑150 (MQ=255)
        gCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATTAAAAAGat                                                                                                                                               <  1:922016/150‑1 (MQ=255)
        gCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGat                                                                                                                                               <  2:3097925/150‑1 (MQ=255)
        gCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGat                                                                                                                                               <  1:384033/150‑1 (MQ=255)
            ttACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATaa                                                                                                                                           >  2:3732984/1‑150 (MQ=255)
             tACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATaaa                                                                                                                                          <  1:4511593/150‑1 (MQ=255)
             tACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATaaa                                                                                                                                          >  1:744990/1‑150 (MQ=255)
              aCGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAAt                                                                                                                                         <  1:2643063/150‑1 (MQ=255)
              aCGACACTATCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATTTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAAt                                                                                                                                         >  2:1025753/1‑150 (MQ=255)
               cGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAAtt                                                                                                                                        <  2:3063759/150‑1 (MQ=255)
                 acacTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTAt                                                                                                                                      <  2:4212914/150‑1 (MQ=255)
                 acacTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTAt                                                                                                                                      >  1:2537724/1‑150 (MQ=255)
                   acTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATgg                                                                                                                                    <  2:489249/150‑1 (MQ=255)
                   acTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATgg                                                                                                                                    >  1:3709446/1‑150 (MQ=255)
                     tctcCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATgggg                                                                                                                                  >  1:3904810/1‑150 (MQ=255)
                       tcCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTg                                                                                                                                <  2:2489319/150‑1 (MQ=255)
                       tcCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTg                                                                                                                                <  1:3020170/150‑1 (MQ=255)
                         ccGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGga                                                                                                                              >  2:708585/1‑150 (MQ=255)
                            aTACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGgaaat                                                                                                                           <  2:684521/150‑1 (MQ=255)
                              aCAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGgaaatag                                                                                                                         <  1:1193801/150‑1 (MQ=255)
                                    aaaTTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATAc                                                                                                                   >  1:1473699/1‑150 (MQ=255)
                                     aaTTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATAcc                                                                                                                  <  2:3960229/150‑1 (MQ=255)
                                      aTTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCa                                                                                                                 >  2:1559001/1‑150 (MQ=255)
                                      aTTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCa                                                                                                                 >  1:1607856/1‑150 (MQ=255)
                                       ttCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCAt                                                                                                                <  1:3940122/150‑1 (MQ=255)
                                         cAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCt                                                                                                              <  1:2804187/150‑1 (MQ=255)
                                         cAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCt                                                                                                              <  1:4463791/150‑1 (MQ=255)
                                            aCATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGt                                                                                                           <  2:3800790/150‑1 (MQ=255)
                                                gggCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTaaaa                                                                                                       >  1:4423266/1‑150 (MQ=255)
                                                gggCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTaaaa                                                                                                       >  1:1635316/1‑150 (MQ=255)
                                                    aGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAAt                                                                                                   <  1:1650657/150‑1 (MQ=255)
                                                     gAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAAtt                                                                                                  <  2:4586417/150‑1 (MQ=255)
                                                      aaaTGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATTAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTc                                                                                                 >  2:1097427/1‑150 (MQ=255)
                                                      aaaTGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATTAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTc                                                                                                 >  2:3422479/1‑150 (MQ=255)
                                                      aaaTGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTc                                                                                                 <  1:1121030/150‑1 (MQ=255)
                                                           tCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTa                                                                                            >  2:1619916/1‑150 (MQ=255)
                                                              gAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCa                                                                                         <  2:2978924/150‑1 (MQ=255)
                                                              gAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCa                                                                                         <  1:3625144/150‑1 (MQ=255)
                                                                  atCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATc                                                                                     <  2:4502896/150‑1 (MQ=255)
                                                                  atCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATc                                                                                     >  2:1729291/1‑150 (MQ=255)
                                                                   tCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATTAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCa                                                                                    <  2:726403/150‑1 (MQ=255)
                                                                   tCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCa                                                                                    <  2:1674507/150‑1 (MQ=255)
                                                                     aaTTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCAtt                                                                                  <  1:886428/150‑1 (MQ=255)
                                                                      aTTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATTAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTa                                                                                 <  1:2125291/150‑1 (MQ=255)
                                                                        tCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTAcc                                                                               <  1:4267087/150‑1 (MQ=255)
                                                                         cGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCa                                                                              >  2:3511831/1‑150 (MQ=255)
                                                                         cGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCa                                                                              <  1:3456432/150‑1 (MQ=255)
                                                                           aaGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGa                                                                            <  1:1170379/150‑1 (MQ=255)
                                                                             gTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAat                                                                          <  1:1345492/150‑1 (MQ=255)
                                                                              tATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAata                                                                         <  2:283457/150‑1 (MQ=255)
                                                                                    aaTACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATAc                                                                   <  2:269937/150‑1 (MQ=255)
                                                                                    aaTACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATAc                                                                   <  1:948745/150‑1 (MQ=255)
                                                                                        cGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATTAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCata                                                               >  1:544624/1‑150 (MQ=255)
                                                                                        cGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCata                                                               <  2:587819/150‑1 (MQ=255)
                                                                                          agaCGCTTAACCCAAAAAAAATGTCACACTCTCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCataat                                                             <  2:1626617/150‑1 (MQ=255)
                                                                                          agaCGCTTAAACCAAAAAAAATGTCACTCACGCCTTATTTAAAGAGGCCACTGCATAATTAAAAATATATAAATTATGGGGTGTAAATAGAAATTCCATCTGTTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCataat                                                             <  2:237584/150‑1 (MQ=255)
                                                                                             cGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATAtt                                                          <  1:1744316/150‑1 (MQ=255)
                                                                                              gCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTg                                                         >  1:2701484/1‑150 (MQ=255)
                                                                                               cTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGa                                                        >  1:2047753/1‑150 (MQ=255)
                                                                                               cTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGa                                                        >  1:1900169/1‑150 (MQ=255)
                                                                                                     ccAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGAttatta                                                  >  2:596436/1‑150 (MQ=255)
                                                                                                      cAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAg                                                 >  2:317858/1‑150 (MQ=255)
                                                                                                        aaaaaaaTGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCa                                               >  1:50202/1‑150 (MQ=255)
                                                                                                           aaaaTGTCACACACGCCTTATTTAAAGAGGCCACTCCCTAATTAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACtt                                            <  1:4381200/150‑1 (MQ=255)
                                                                                                            aaaTGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACttt                                           <  2:3761515/150‑1 (MQ=255)
                                                                                                             aaTTTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATTAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTta                                          <  1:4337009/150‑1 (MQ=255)
                                                                                                              aTGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTATCAGAATATAATACCATAATATTGATTATTAGCACTTtat                                         <  1:896159/150‑1 (MQ=255)
                                                                                                              aTGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTtat                                         <  2:3581558/150‑1 (MQ=255)
                                                                                                               tGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTtata                                        <  2:3056260/150‑1 (MQ=255)
                                                                                                               tGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTtata                                        <  1:1723762/150‑1 (MQ=255)
                                                                                                                 tCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAAt                                      <  1:630190/150‑1 (MQ=255)
                                                                                                                   acacacGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCa                                    >  1:1228553/1‑150 (MQ=255)
                                                                                                                    cacacGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCAt                                   <  2:1794848/150‑1 (MQ=255)
                                                                                                                     acacGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCAGAATATTGATTATTAGCACTTTATAATCAtt                                  <  1:2461863/150‑1 (MQ=255)
                                                                                                                      cacGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTg                                 >  1:3785442/1‑150 (MQ=255)
                                                                                                                      cacGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTg                                 <  2:1418271/150‑1 (MQ=255)
                                                                                                                        cGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGaa                               <  2:3243621/150‑1 (MQ=255)
                                                                                                                             tATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATaaaa                          <  2:1488238/150‑1 (MQ=255)
                                                                                                                             tATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATaaaa                          <  1:3340653/150‑1 (MQ=255)
                                                                                                                                 tAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAg                      <  2:783230/150‑1 (MQ=255)
                                                                                                                                  aaaGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATGACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGa                     <  2:1160493/150‑1 (MQ=255)
                                                                                                                                    agagGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGAtt                   <  1:1486382/150‑1 (MQ=255)
                                                                                                                                       ggCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTa                <  1:791075/150‑1 (MQ=255)
                                                                                                                                       ggCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTa                <  1:2271490/150‑1 (MQ=255)
                                                                                                                                         ccACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATg              <  1:2913921/150‑1 (MQ=255)
                                                                                                                                         ccACTGCCTAATGAAAAAGATAGAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATg              <  2:2443115/150‑1 (MQ=255)
                                                                                                                                           aCTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTa            >  1:3095247/1‑150 (MQ=255)
                                                                                                                                              gCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTACtt         >  1:2391298/1‑150 (MQ=255)
                                                                                                                                               ccTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTACttt        <  1:708585/150‑1 (MQ=255)
                                                                                                                                                cTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTACtttt       <  2:3695875/150‑1 (MQ=255)
                                                                                                                                                cTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTACtttt       <  1:1354296/150‑1 (MQ=255)
                                                                                                                                                 tAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTACTTTTa      <  2:3468424/150‑1 (MQ=255)
                                                                                                                                                   aTGAAAAAGATATAAATTATGGGGTGTAAATAGAAATAACATCTGGTAAAAGAATTAGCTTATCAAATCATTACCAGAATATAATAACATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTACTTTTaaa    <  2:2088653/150‑1 (MQ=255)
                                                                                                                                                   aTGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTACTTTTaaa    >  1:3909574/1‑150 (MQ=255)
                                                                                                                                                   aTGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTACTTTTaaa    <  2:2088678/150‑1 (MQ=255)
                                                                                                                                                   aTGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTACTTTTaaa    >  2:1896068/1‑150 (MQ=255)
                                                                                                                                                    tGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTACTTGTaaaa   >  2:3485415/1‑150 (MQ=255)
                                                                                                                                                     gAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTACTTTTAAAAc  <  2:2179406/150‑1 (MQ=255)
                                                                                                                                                     gAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTACTTTTAAAAc  >  2:3551839/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGTTAAAGCCTTTACGACACTCTCCCGATACAATAAAATTCAAACATGGGCAGAAATGTCTGAATATCAATTCGAAGTATTTCAATACGAGACGCTTAACCCAAAAAAAATGTCACACACGCCTTATTTAAAGAGGCCACTGCCTAATGAAAAAGATATAAATTATGGGGTGGAAATAGAAATACCATCTGGTAAAAGAATTCGCTTATCAAATCATTACCAGAATATAATACCATAATATTGATTATTAGCACTTTATAATCATTGAATAAAAATAGATTTTATGTACTTTTAAAAC  >  CP009273/3646835‑3647133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑