BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,649,637	G→A	38.3%	L6F (CTC→TTC)	hdeB ←	acid‑resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,649,637	0	G	A	38.3%	225.9 / 258.5	222	L6F (CTC→TTC)	hdeB	acid‑resistance protein
Reads supporting (aligned to +/- strand): ref base G (74/63); new base A (36/49); total (110/112)
Fisher's exact test for biased strand distribution p-value = 9.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 236 total aligned reads displayed.

CCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATATCTTTCTTAATT  >  CP009273/3649488‑3649786

ccaTGCAACCGGGGTCATTGCTTTTTGATTCAGATCAATTAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCTACGCATATTGTGCGTTCACCAGTGACAATGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGgag                                                                                                                                                       <  2:3294704/150‑1 (MQ=255)

  aTGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGTCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGgagag                                                                                                                                                     >  1:1834309/1‑150 (MQ=255)

  aTGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGgagag                                                                                                                                                     <  2:4171350/150‑1 (MQ=255)

   tGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGgagaga                                                                                                                                                    >  1:3335459/1‑150 (MQ=255)

   tGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGgaaaga                                                                                                                                                    <  1:3149371/150‑1 (MQ=255)

    gCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGAt                                                                                                                                                   <  2:2632028/150‑1 (MQ=255)

     cAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATg                                                                                                                                                  >  2:1521896/1‑150 (MQ=255)

     cAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATg                                                                                                                                                  >  2:4424268/1‑150 (MQ=255)

     cAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATg                                                                                                                                                  <  1:815963/150‑1 (MQ=255)

     cAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATg                                                                                                                                                  >  1:4613665/1‑150 (MQ=255)

     cAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATg                                                                                                                                                  <  1:426360/150‑1 (MQ=255)

          ggggTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAata                                                                                                                                             >  1:1302943/1‑150 (MQ=255)

          ggggTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAata                                                                                                                                             >  2:2609595/1‑150 (MQ=255)

          ggggTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAata                                                                                                                                             >  2:4381572/1‑150 (MQ=255)

             gTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTc                                                                                                                                          >  1:2477788/1‑150 (MQ=255)

                aTTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCAtt                                                                                                                                       >  2:4315074/1‑150 (MQ=255)

                aTTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCAtt                                                                                                                                       >  2:3061864/1‑150 (MQ=255)

                  ttcTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCAtttt                                                                                                                                     <  1:4456513/148‑1 (MQ=255)

                  tGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCAtttt                                                                                                                                     <  1:1735477/150‑1 (MQ=255)

                    cTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGt                                                                                                                                   >  1:2150190/1‑150 (MQ=255)

                     ttttGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTa                                                                                                                                  >  1:1573155/1‑150 (MQ=255)

                     ttttGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTa                                                                                                                                  >  1:4076979/1‑150 (MQ=255)

                      tttGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTaa                                                                                                                                 <  2:2709724/150‑1 (MQ=255)

                         ggATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAAccc                                                                                                                              >  1:2767139/1‑150 (MQ=255)

                          gATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCa                                                                                                                             <  2:3925474/150‑1 (MQ=255)

                            ttCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCAtt                                                                                                                           >  1:357536/1‑150 (MQ=255)

                             tCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTGActggagt                                                                                                                          <  1:3815228/150‑7 (MQ=255)

                             tCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTc                                                                                                                          <  2:3287307/150‑1 (MQ=255)

                              cAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCa                                                                                                                         >  2:1121132/1‑150 (MQ=255)

                              cAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTGTACGGAGAGATGAAATATTCATTTTGTAACCCATTCa                                                                                                                         >  2:3716606/1‑150 (MQ=255)

                                gATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATCTTCATTTTGTAACCCATTCAat                                                                                                                       >  2:4326939/1‑150 (MQ=255)

                                  tCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAatat                                                                                                                     >  1:2948974/1‑150 (MQ=255)

                                    aaTAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATag                                                                                                                   <  2:3667830/150‑1 (MQ=255)

                                       aaaTTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAgaga                                                                                                                >  1:2939394/1‑150 (MQ=255)

                                         aTTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGAtt                                                                                                              <  1:1223662/150‑1 (MQ=255)

                                         aTTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGAtt                                                                                                              >  1:1682962/1‑150 (MQ=255)

                                          ttCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATta                                                                                                             >  2:2580760/1‑150 (MQ=255)

                                             cTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATtatat                                                                                                          <  1:3746163/150‑1 (MQ=255)

                                                 cAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGa                                                                                                      >  2:2140897/1‑150 (MQ=255)

                                                      cATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACagg                                                                                                 <  2:852338/150‑1 (MQ=255)

                                                      cATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACagg                                                                                                 >  2:1959975/1‑150 (MQ=255)

                                                       atatCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACagga                                                                                                >  1:2524938/1‑150 (MQ=255)

                                                        tatCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACaggag                                                                                               <  1:821083/150‑1 (MQ=255)

                                                             ttAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATa                                                                                          <  2:90654/150‑1 (MQ=255)

                                                              tAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAg                                                                                         >  1:2069791/1‑150 (MQ=255)

                                                               aGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAgc                                                                                        >  2:1010218/1‑150 (MQ=255)

                                                                gCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAgct                                                                                       <  2:3535125/150‑1 (MQ=255)

                                                                  ggATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAgctgc                                                                                     >  1:49529/1‑150 (MQ=255)

                                                                  ggATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAgctgc                                                                                     >  2:3410752/1‑150 (MQ=255)

                                                                  ggATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAgctgc                                                                                     >  2:3410459/1‑150 (MQ=255)

                                                                   gATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAgctgct                                                                                    <  1:3055907/150‑1 (MQ=255)

                                                                    aTTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCtt                                                                                   <  1:3924342/150‑1 (MQ=255)

                                                                       cATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTa                                                                                <  2:919810/150‑1 (MQ=255)

                                                                        aTTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGACTAGCTGCTTTTaa                                                                               >  2:1632960/1‑150 (MQ=255)

                                                                        aTTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTaa                                                                               <  1:3939304/150‑1 (MQ=255)

                                                                              tGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTACagaaga                                                                         <  2:2102722/150‑1 (MQ=255)

                                                                              tGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAagaaga                                                                         <  2:184591/150‑1 (MQ=255)

                                                                              tGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAagaaga                                                                         >  2:489349/1‑150 (MQ=255)

                                                                              tGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAagaaga                                                                         <  2:3884012/150‑1 (MQ=255)

                                                                               gCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGat                                                                        <  2:2726201/150‑1 (MQ=255)

                                                                                ccAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGata                                                                       <  1:517785/150‑1 (MQ=255)

                                                                                 cAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGatat                                                                      <  1:3805172/150‑1 (MQ=255)

                                                                                       aGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGc                                                                >  2:1796942/1‑150 (MQ=255)

                                                                                       aGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGc                                                                >  1:4485454/1‑150 (MQ=255)

                                                                                         aTTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCt                                                              <  2:1369689/150‑1 (MQ=255)

                                                                                         aTTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCt                                                              <  1:2596056/150‑1 (MQ=255)

                                                                                            gtgCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGACTAGCTGCTTTTAAAGAAGATATTCCGGCCTGtt                                                           >  2:4588016/1‑150 (MQ=255)

                                                                                            gagCGTACACCAGTCACAAAGCCGCTACAGCGCCCATAAAAATAAACGCATTACGCAGAGATGAAATATACATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGtt                                                           <  1:3306852/148‑1 (MQ=255)

                                                                                             tgCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTc                                                          <  2:124780/150‑1 (MQ=255)

                                                                                             tgCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTc                                                          >  1:1853988/1‑150 (MQ=255)

                                                                                                gTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAat                                                       >  2:1623081/1‑150 (MQ=255)

                                                                                                gTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAat                                                       >  2:2959401/1‑150 (MQ=255)

                                                                                                   cACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATg                                                    <  1:92169/150‑1 (MQ=255)

                                                                                                      cAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCaa                                                 >  1:1559189/1‑150 (MQ=255)

                                                                                                         tGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGa                                              <  1:633020/150‑1 (MQ=255)

                                                                                                           aCAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAg                                            >  1:3682403/1‑150 (MQ=255)

                                                                                                                 ccGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGAt                                      <  1:1110980/150‑1 (MQ=255)

                                                                                                                  cGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATg                                     >  1:1930494/1‑150 (MQ=255)

                                                                                                                  cGCTACAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATg                                     <  2:4210490/150‑1 (MQ=255)

                                                                                                                      aCAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAAc                                 <  1:3190897/150‑1 (MQ=255)

                                                                                                                       cAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACg                                >  2:3587329/1‑150 (MQ=255)

                                                                                                                       cAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACg                                <  2:3906698/150‑1 (MQ=255)

                                                                                                                       cAGCGCCCATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACg                                <  2:4136788/150‑1 (MQ=255)

                                                                                                                       cAGCGCACATAAAAATAAACGCTTTACGTAGATATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACg                                <  2:721259/150‑1 (MQ=255)

                                                                                                                           gcCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCAtt                            <  2:57393/150‑1 (MQ=255)

                                                                                                                           gcCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCAtt                            <  1:58315/150‑1 (MQ=255)

                                                                                                                             ccATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTcc                          >  1:300054/1‑150 (MQ=255)

                                                                                                                              cATAAAAATAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTccc                         <  1:721715/150‑1 (MQ=255)

                                                                                                                                  aaaaTAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGACTAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGaa                     >  2:4519731/1‑150 (MQ=255)

                                                                                                                                    aaTAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAAtt                   <  1:1233239/150‑1 (MQ=255)

                                                                                                                                     aTAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTa                  <  1:2956026/150‑1 (MQ=255)

                                                                                                                                      tAAACGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTAc                 >  2:2210633/1‑150 (MQ=255)

                                                                                                                                       aaaCGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGACTAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACa                >  1:2641224/1‑150 (MQ=255)

                                                                                                                                       aaaCGCTTTACGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACa                >  1:2510240/1‑150 (MQ=255)

                                                                                                                                          cGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACatat             <  2:946267/150‑1 (MQ=255)

                                                                                                                                           gCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATAtc            >  2:3075451/1‑150 (MQ=255)

                                                                                                                                                 cGGAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATAtatttctt      >  1:4131964/1‑150 (MQ=255)

                                                                                                                                                  ggAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATATCTTTCTTa     >  1:4401602/1‑150 (MQ=255)

                                                                                                                                                  ggAAAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATATCTTTCTTa     >  1:1616101/1‑150 (MQ=255)

                                                                                                                                                     gagaTGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATATCTTTCTTAAtt  <  2:2477788/150‑1 (MQ=255)

CCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTACGATGTAACGCATTCCCGGAATTACATATCTTTCTTAATT  >  CP009273/3649488‑3649786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`