Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,694,707 C→A 21.4% S262* (TCG→TAG)  yhjV → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,694,7070CA21.4% 744.2 / 141.8 275S262* (TCG→TAG) yhjVputative transporter
Reads supporting (aligned to +/- strand):  ref base C (126/89);  new base A (21/38);  total (148/127)
Fisher's exact test for biased strand distribution p-value = 1.95e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

TTCTGCAGTATTTATTCAGGTACTTAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGCTTAATATCTTTGCCGTACTGACCGCATTCT  >  CP009273/3694558‑3694856
                                                                                                                                                     |                                                                                                                                                     
ttCTGCAGTATTTATTCAGGTACTTAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTc                                                                                                                                                       >  2:4583974/1‑150 (MQ=255)
   tGCAGTATTTATTCAGGTACTTAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGtt                                                                                                                                                    <  2:1850190/150‑1 (MQ=255)
    gCAGTATTTATTCAGGTACTTAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGttt                                                                                                                                                   >  1:2723633/1‑150 (MQ=255)
        tatttattCAGGTACTTAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCt                                                                                                                                               >  1:548229/1‑150 (MQ=255)
        tatttattCAGGTACTTAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCt                                                                                                                                               <  1:317052/150‑1 (MQ=255)
        tatttattCAGGTACTTAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGGGATCCTGTTTTTTGCCTTTTCGTTTACCt                                                                                                                                               >  1:740089/1‑150 (MQ=255)
         atttattCAGGTACTTAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCtt                                                                                                                                              >  1:2162075/1‑150 (MQ=255)
             attCAGGTACTTAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCa                                                                                                                                          <  1:3649501/150‑1 (MQ=255)
               tCAGGTACTTAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAAt                                                                                                                                        >  2:3905906/1‑150 (MQ=255)
               tCAGGTACTTAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAAt                                                                                                                                        <  2:1373271/150‑1 (MQ=255)
                  ggTACTTAAACCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGAACTGGCAACCCGGCTCGCGATGCGAACCCACCGAATTAGTAATATCACGCTCATCGCGGTGATCATGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAg                                                                                                                                     >  2:249751/1‑150 (MQ=255)
                   gTACTTAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGc                                                                                                                                    >  1:3547440/1‑150 (MQ=255)
                    tACTTAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGcc                                                                                                                                   <  2:200436/150‑1 (MQ=255)
                        tAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACga                                                                                                                               >  1:2588216/1‑150 (MQ=255)
                           cccAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACgaaga                                                                                                                            <  2:2157798/150‑1 (MQ=255)
                            ccAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACgaagaa                                                                                                                           <  1:3082752/150‑1 (MQ=255)
                            ccAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACgaagaa                                                                                                                           <  1:4511556/150‑1 (MQ=255)
                             cAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGaagaag                                                                                                                          <  2:335145/150‑1 (MQ=255)
                             cAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGaagaag                                                                                                                          <  2:2804880/150‑1 (MQ=255)
                              aaTGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGc                                                                                                                         >  2:2678574/1‑150 (MQ=255)
                               aTGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGcc                                                                                                                        <  1:489477/150‑1 (MQ=255)
                                tGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCg                                                                                                                       >  2:2585207/1‑150 (MQ=255)
                                tGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCg                                                                                                                       <  1:2393152/150‑1 (MQ=255)
                                tGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCg                                                                                                                       <  1:1853017/150‑1 (MQ=255)
                                tGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCg                                                                                                                       <  1:3566480/150‑1 (MQ=255)
                                  aaTATTGCCTATCGTCAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGtt                                                                                                                     <  2:3024507/150‑1 (MQ=255)
                                  aaTATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGtt                                                                                                                     >  2:1974102/1‑150 (MQ=255)
                                  aaTATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGtt                                                                                                                     <  1:3414195/150‑1 (MQ=255)
                                   atatTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGttt                                                                                                                    >  1:4491983/1‑150 (MQ=255)
                                     atTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCt                                                                                                                  <  1:2866607/150‑1 (MQ=255)
                                      ttGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTg                                                                                                                 <  1:1425750/150‑1 (MQ=255)
                                       tGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGc                                                                                                                >  1:4533130/1‑150 (MQ=255)
                                         ccTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCt                                                                                                              <  1:2544371/150‑1 (MQ=255)
                                          cgaTCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGAGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAATCCGTTTCTGCAtt                                                                                                             >  2:2923287/3‑150 (MQ=255)
                                           tATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGACACGAAGAAGCCGTTTCTGCCttt                                                                                                            >  2:2042469/1‑150 (MQ=255)
                                               gTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAAc                                                                                                        <  1:2044903/150‑1 (MQ=255)
                                               gTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAAc                                                                                                        >  1:2978890/1‑150 (MQ=255)
                                                  aaCGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACaaa                                                                                                     >  1:1491425/1‑150 (MQ=255)
                                                       gAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATAtc                                                                                                >  2:501566/1‑150 (MQ=255)
                                                        aaGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATAtct                                                                                               >  2:2477068/1‑150 (MQ=255)
                                                        aaGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATAtct                                                                                               >  2:3322945/1‑150 (MQ=255)
                                                           cGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAg                                                                                            <  1:16079/150‑1 (MQ=255)
                                                           cGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAg                                                                                            >  1:3009894/1‑150 (MQ=255)
                                                              aTAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCAc                                                                                         <  2:1626972/150‑1 (MQ=255)
                                                               tAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACt                                                                                        <  1:3285485/150‑1 (MQ=255)
                                                                  aGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGgc                                                                                     <  1:4313679/150‑1 (MQ=255)
                                                                   gTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACATAATATCTCAGCACTGgcg                                                                                    <  2:4256145/150‑1 (MQ=255)
                                                                   gTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGgcg                                                                                    >  1:1019439/1‑150 (MQ=255)
                                                                      cTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTg                                                                                 >  2:3931054/1‑150 (MQ=255)
                                                                      cTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTg                                                                                 >  2:2327393/1‑150 (MQ=255)
                                                                        ggCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGc                                                                               <  1:1354538/150‑1 (MQ=255)
                                                                        ggCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGc                                                                               >  2:1466163/1‑150 (MQ=255)
                                                                           aaCCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCcgc                                                                            >  1:2323099/1‑150 (MQ=255)
                                                                           aaCCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCcgc                                                                            >  1:1695799/1‑150 (MQ=255)
                                                                              ccGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCa                                                                         <  1:1766133/150‑1 (MQ=255)
                                                                               cGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAg                                                                        <  1:4547323/150‑1 (MQ=255)
                                                                               cGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAg                                                                        <  1:1572546/150‑1 (MQ=255)
                                                                               cGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAg                                                                        <  2:2553165/150‑1 (MQ=255)
                                                                                  cTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTg                                                                     <  1:845406/150‑1 (MQ=255)
                                                                                   tCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGa                                                                    <  2:4538949/150‑1 (MQ=255)
                                                                                   tCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGa                                                                    >  2:2760689/1‑150 (MQ=255)
                                                                                    cgcgCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGAt                                                                   >  1:361544/1‑150 (MQ=255)
                                                                                    cgcgCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCAGGCCGCGCAGGTGAt                                                                   >  1:4047021/1‑150 (MQ=255)
                                                                                     gcgcTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCGATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATc                                                                  >  2:102326/1‑150 (MQ=255)
                                                                                      cgcTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATcc                                                                 >  1:1043835/1‑150 (MQ=255)
                                                                                        cTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCt                                                               >  2:3977976/1‑150 (MQ=255)
                                                                                        cTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCt                                                               >  2:4453965/1‑150 (MQ=255)
                                                                                         tGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTg                                                              >  1:3693260/1‑150 (MQ=255)
                                                                                         tGCGTACCCACCGAAGTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTg                                                              <  2:398791/150‑1 (MQ=255)
                                                                                              aCCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCat                                                         <  2:284086/150‑1 (MQ=255)
                                                                                               cccACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCAGGGCata                                                        >  1:410388/1‑150 (MQ=255)
                                                                                                  aCCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGTCATAtca                                                     >  2:3802518/1‑150 (MQ=255)
                                                                                                     gAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATAtcattc                                                  <  1:2281330/150‑1 (MQ=255)
                                                                                                     gAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATAtcattc                                                  <  2:4027785/150‑1 (MQ=255)
                                                                                                         tAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGTTGATCCCTGGGCATATCATTCatat                                              >  2:3080327/1‑150 (MQ=255)
                                                                                                            ttATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCAc                                           <  2:552405/150‑1 (MQ=255)
                                                                                                              atatCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACct                                         <  2:3142966/150‑1 (MQ=255)
                                                                                                              atatCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACct                                         <  2:4300807/150‑1 (MQ=255)
                                                                                                               tatCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACctc                                        >  2:3862706/1‑150 (MQ=255)
                                                                                                               tatCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACctc                                        >  2:3862581/1‑150 (MQ=255)
                                                                                                                atCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACctct                                       <  2:2972777/150‑1 (MQ=255)
                                                                                                                 tCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTa                                      <  1:1351384/150‑1 (MQ=255)
                                                                                                                 tCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTa                                      >  1:3023299/1‑150 (MQ=255)
                                                                                                                   aCGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACg                                    <  1:4112009/150‑1 (MQ=255)
                                                                                                                    cGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACgg                                   >  1:3599433/1‑150 (MQ=255)
                                                                                                                     gCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGt                                  <  2:339530/150‑1 (MQ=255)
                                                                                                                     gCTCATCGCGGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGt                                  <  2:3292815/150‑1 (MQ=255)
                                                                                                                       tCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGc                                >  1:3807749/1‑150 (MQ=255)
                                                                                                                           cgcgGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGCTTaa                            >  2:377062/1‑150 (MQ=255)
                                                                                                                           cgcgGTGATCCTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGCTTaa                            <  2:1087061/150‑1 (MQ=255)
                                                                                                                            gcgGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGCTTAat                           <  1:2059610/150‑1 (MQ=255)
                                                                                                                                  aTCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGCTTAATATCttt                     <  1:641361/150‑1 (MQ=255)
                                                                                                                                  aTCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGCTTAATATCttt                     >  2:1692730/1‑150 (MQ=255)
                                                                                                                                     cTGTTTTTTGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGCTTAATATCTTTGcc                  <  2:3595895/150‑1 (MQ=255)
                                                                                                                                       gTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGCTTAATATCTTTGCCGt                >  1:1166482/1‑150 (MQ=255)
                                                                                                                                             tGCCTTTTAGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGCTTAATATCTTTGCCGTACTGAc          <  1:3512799/150‑1 (MQ=255)
                                                                                                                                                   ttCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGCTTAATATCTTTGCCGTACTGACCGCAtt    <  2:1557921/150‑1 (MQ=255)
                                                                                                                                                     cGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGCTTAATATCTTTGCCGTACTGACCGCATTCt  >  1:1915975/1‑150 (MQ=255)
                                                                                                                                                     cGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGCTTAATATCTTTGCCGTACTGACCGCATTCt  >  1:3549720/1‑150 (MQ=255)
                                                                                                                                                     cGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGCTTAATATCTTTGCCGTACTGACCGCATTCt  <  2:821605/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCTGCAGTATTTATTCAGGTACTTAACCCAATGAATATTGCCTATCGTAAACGGGAAGCGGATAAAGTACTGGCAACCCGGCTCGCGCTGCGTACCCACCGAATTAGTTATATCACGCTCATCGCGGTGATCCTGTTTTTTGCCTTTTCGTTTACCTTCTCAATTAGCCACGAAGAAGCCGTTTCTGCCTTTGAACAAAATATCTCAGCACTGGCGCTGGCCGCGCAGGTGATCCCTGGGCATATCATTCATATCACCTCTACGGTGCTTAATATCTTTGCCGTACTGACCGCATTCT  >  CP009273/3694558‑3694856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑