Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,761,602 G→A 32.3% V22V (GTG→GTA)  yibG → TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,761,6020GA32.3% 340.7 / 226.3 239V22V (GTG→GTA) yibGTPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base G (91/70);  new base A (40/37);  total (131/108)
Fisher's exact test for biased strand distribution p-value = 5.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-02

Only 100 of 248 total aligned reads displayed.

CATATGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTACTAAAAA  >  CP009273/3761455‑3761751
                                                                                                                                                   |                                                                                                                                                     
cATATGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTaaa                                                                                                                                                     <  1:1775833/150‑1 (MQ=255)
 atatGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGaaa                                                                                                                                                    >  2:3444306/1‑150 (MQ=255)
      ggCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATaaa                                                                                                                                               >  2:3158237/1‑150 (MQ=255)
      ggCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATaaa                                                                                                                                               >  2:4010948/1‑150 (MQ=255)
        ccATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaac                                                                                                                                             <  1:102335/150‑1 (MQ=255)
        ccATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaac                                                                                                                                             >  2:1927240/1‑150 (MQ=255)
         cATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATaaaaca                                                                                                                                            >  2:820791/1‑150 (MQ=255)
          aTCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATaaaacaa                                                                                                                                           >  1:108682/1‑150 (MQ=255)
           tCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaacaaa                                                                                                                                          <  2:1122055/150‑1 (MQ=255)
           tCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaacaaa                                                                                                                                          <  2:4364541/150‑1 (MQ=255)
            cTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaacaaaa                                                                                                                                         >  2:3657635/1‑150 (MQ=255)
            cTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATaaaacaaaa                                                                                                                                         >  1:3169997/1‑150 (MQ=255)
                gCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGaa                                                                                                                                     <  2:3764251/150‑1 (MQ=255)
                gCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGaa                                                                                                                                     <  2:4128934/150‑1 (MQ=255)
                 cACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGaaa                                                                                                                                    >  1:3182914/1‑150 (MQ=255)
                 cACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGaaa                                                                                                                                    <  1:761376/150‑1 (MQ=255)
                    cccATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTa                                                                                                                                 >  1:858025/1‑150 (MQ=255)
                            tAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATgggg                                                                                                                         <  2:2535677/150‑1 (MQ=255)
                             aaTATGCATGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGt                                                                                                                        <  1:3304121/150‑1 (MQ=255)
                                   cAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAg                                                                                                                  >  2:4213185/1‑150 (MQ=255)
                                       gTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAAt                                                                                                              <  2:4312317/150‑1 (MQ=255)
                                          cGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGAc                                                                                                           <  2:2911766/150‑1 (MQ=255)
                                             cAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTa                                                                                                        >  1:397918/1‑150 (MQ=255)
                                               gAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATg                                                                                                      <  1:1905874/150‑1 (MQ=255)
                                                   aaCCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGAttt                                                                                                  >  2:1619725/1‑150 (MQ=255)
                                                     cccAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTaa                                                                                                <  2:404063/150‑1 (MQ=255)
                                                        aGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTg                                                                                             <  1:4325117/150‑1 (MQ=255)
                                                         gTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTga                                                                                            >  1:1255006/1‑150 (MQ=255)
                                                         gTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTga                                                                                            <  2:698405/150‑1 (MQ=255)
                                                           gATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTgaag                                                                                          >  2:1321918/1‑150 (MQ=255)
                                                            atatAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTgaaga                                                                                         <  1:1685691/150‑1 (MQ=255)
                                                            atatAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTgaaga                                                                                         >  2:1231145/1‑150 (MQ=255)
                                                              ataAGATAGGTTGTAGATGTATTAAAGAATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGaaa                                                                                       <  1:2706776/150‑1 (MQ=255)
                                                              ataAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGaaa                                                                                       >  1:2492332/1‑150 (MQ=255)
                                                                aaGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATTAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAg                                                                                     <  2:261998/150‑1 (MQ=255)
                                                                aaGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAg                                                                                     >  1:1878239/1‑150 (MQ=255)
                                                                  gATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGtt                                                                                   >  1:1570878/1‑150 (MQ=255)
                                                                    tAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCCATGACCTATGATTTAAGTGAAGAAAAGTtga                                                                                 >  2:3194850/1‑150 (MQ=255)
                                                                    tAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTtga                                                                                 >  2:559858/1‑150 (MQ=255)
                                                                     aGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTTCATGGTGCTGATGTGAAAATAAAACAAAAAGAAAGTATTATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTtgat                                                                                >  2:2226066/1‑150 (MQ=255)
                                                                     aGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTtgat                                                                                <  2:1817270/150‑1 (MQ=255)
                                                                       gTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTtgatga                                                                              <  1:4270852/150‑1 (MQ=255)
                                                                        ttGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGaa                                                                             >  1:2028612/1‑150 (MQ=255)
                                                                          gTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAAc                                                                           >  1:264498/1‑150 (MQ=255)
                                                                          gTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAAc                                                                           <  1:2357515/150‑1 (MQ=255)
                                                                           tAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACt                                                                          >  1:4546031/1‑150 (MQ=255)
                                                                            ataTGTATGAAAGCATGCTGGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTg                                                                         <  1:2858498/148‑1 (MQ=255)
                                                                                  aTGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGtat                                                                   >  1:3807477/1‑150 (MQ=255)
                                                                                   tGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGtata                                                                  <  1:2808803/150‑1 (MQ=255)
                                                                                    gAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATaa                                                                 >  1:3826441/1‑150 (MQ=255)
                                                                                    gAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATaa                                                                 >  1:2055649/1‑150 (MQ=255)
                                                                                       atcATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATc                                                              >  1:4353099/3‑150 (MQ=255)
                                                                                        gcttGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATca                                                             <  2:3480161/147‑1 (MQ=255)
                                                                                               tGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATg                                                      >  2:1447334/1‑150 (MQ=255)
                                                                                                  tACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTg                                                   <  2:1094257/150‑1 (MQ=255)
                                                                                                     tATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGAtt                                                >  1:4056077/1‑150 (MQ=255)
                                                                                                      aTTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTc                                               <  1:534829/150‑1 (MQ=255)
                                                                                                        ttttttATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCag                                             <  2:1653728/150‑1 (MQ=255)
                                                                                                            ttATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGc                                         >  2:2798366/1‑150 (MQ=255)
                                                                                                            ttATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGc                                         <  1:2950801/150‑1 (MQ=255)
                                                                                                               tttCTCTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGcatc                                      <  1:2307019/150‑1 (MQ=255)
                                                                                                                 tctcTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGcatcat                                    >  1:2501283/1‑150 (MQ=255)
                                                                                                                 tctcTTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGcatcat                                    >  2:1804676/1‑150 (MQ=255)
                                                                                                                  ctctTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCAtt                                   <  2:3828991/150‑1 (MQ=255)
                                                                                                                   tctTTTATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCAttt                                  <  2:479101/150‑1 (MQ=255)
                                                                                                                    ctTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGGTTGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTc                                 <  1:290835/150‑1 (MQ=255)
                                                                                                                     ttttATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCg                                >  1:2084692/1‑150 (MQ=255)
                                                                                                                       ttATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGAc                              >  2:3626578/1‑150 (MQ=255)
                                                                                                                       ttATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGAc                              >  1:3521826/1‑150 (MQ=255)
                                                                                                                       ttATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGAc                              >  2:84654/1‑150 (MQ=255)
                                                                                                                       ttATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGAc                              >  2:1428015/1‑150 (MQ=255)
                                                                                                                       ttATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGAc                              >  2:12198/1‑150 (MQ=255)
                                                                                                                       ttATTTGTCAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGAc                              >  1:2478751/1‑150 (MQ=255)
                                                                                                                        tATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACt                             >  2:2037044/1‑150 (MQ=255)
                                                                                                                          tttGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACtat                           <  2:1330824/150‑1 (MQ=255)
                                                                                                                           ttGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACtata                          <  2:3853039/150‑1 (MQ=255)
                                                                                                                           ttGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACtata                          >  1:478287/1‑150 (MQ=255)
                                                                                                                            tGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTatat                         <  2:321219/150‑1 (MQ=255)
                                                                                                                            tGTCAATTGCAGGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTatat                         <  2:603561/150‑1 (MQ=255)
                                                                                                                             gTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATc                        >  1:3444041/1‑150 (MQ=255)
                                                                                                                               cAATTGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCaa                      >  2:4277311/1‑150 (MQ=255)
                                                                                                                                aaTTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAat                     >  1:185847/1‑150 (MQ=255)
                                                                                                                                aaTTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAat                     >  1:223059/1‑150 (MQ=255)
                                                                                                                                  ttGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAatat                   <  1:2454298/150‑1 (MQ=255)
                                                                                                                                  ttGCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAatat                   <  1:4386337/150‑1 (MQ=255)
                                                                                                                                    gCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATAAATATTa                 >  2:1014587/1‑150 (MQ=255)
                                                                                                                                    gCATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTa                 <  1:3555884/150‑1 (MQ=255)
                                                                                                                                     cATGGTGCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTAc                >  1:3520674/1‑150 (MQ=255)
                                                                                                                                         gTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAATTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCt            >  2:4179998/1‑150 (MQ=255)
                                                                                                                                         gTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCt            >  1:969699/1‑150 (MQ=255)
                                                                                                                                          tGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCtt           <  1:147319/150‑1 (MQ=255)
                                                                                                                                           gCTGATGTAAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCttt          <  2:3406902/150‑1 (MQ=255)
                                                                                                                                            cTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTa         <  2:4468473/150‑1 (MQ=255)
                                                                                                                                            cTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTa         <  2:4468871/150‑1 (MQ=255)
                                                                                                                                               aTGTGAAAATAAAACAAAACGAAAGTATGTTGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTACTa      <  2:3720977/150‑1 (MQ=255)
                                                                                                                                               aTGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTACTa      >  2:2476340/1‑150 (MQ=255)
                                                                                                                                               aTGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTACTa      <  2:2689049/150‑1 (MQ=255)
                                                                                                                                                tgtaAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTACTaa     >  1:2788768/1‑150 (MQ=255)
                                                                                                                                                  tgAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTACTaaaa   <  1:4540797/150‑1 (MQ=255)
                                                                                                                                                   gAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTACTaaaaa  >  2:1174895/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CATATGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTACAGCAATGACCTATGATTTAAGTGAAGAAAAGTTGATGAAACTGAAGTATAAATCACAACATGGTGATTCAGAGGCATCATTTCGACTATATCAATATTACTGCTTTACTAAAAA  >  CP009273/3761455‑3761751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑