Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,799,227 G→T 12.8% V28V (GTC→GTA)  waaP ← kinase that phosphorylates core heptose of lipopolysaccharide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,799,2270GT12.8% 758.3 / 56.9 226V28V (GTC→GTA) waaPkinase that phosphorylates core heptose of lipopolysaccharide
Reads supporting (aligned to +/- strand):  ref base G (104/92);  new base T (20/10);  total (124/102)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

ACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCAT  >  CP009273/3799079‑3799376
                                                                                                                                                    |                                                                                                                                                     
aCTCTCTGTCTGCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGa                                                                                                                                                      <  2:2276687/150‑1 (MQ=255)
    tctgtctgCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTc                                                                                                                                                  >  2:1569973/1‑150 (MQ=255)
     ctgtctgCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTGTCCCGGACAGTTCTAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCg                                                                                                                                                 <  2:1776189/150‑1 (MQ=255)
     ctgtctgAGCCAAAAACGGGCATAAGCAATGATATTAGATTTTTTATAATCACTTTTAATGTCGTACCCTTGTGCCATTTAAGAAAATAGATTTTCCCGGACAGTTCAAATCGTAATGTACGACGATTCTCCAGTTCACGAAAGACCTCg                                                                                                                                                 <  2:2164574/150‑1 (MQ=255)
       gtctgCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGcc                                                                                                                                               <  2:2967453/150‑1 (MQ=255)
        tctgCGCCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCg                                                                                                                                              >  2:3169223/1‑150 (MQ=255)
          tgCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTACTGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGtt                                                                                                                                            <  2:2658736/150‑1 (MQ=255)
           gcgcCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTc                                                                                                                                           <  1:2255660/150‑1 (MQ=255)
            cgcCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCa                                                                                                                                          <  2:4443296/150‑1 (MQ=255)
             gcCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAg                                                                                                                                         >  1:1338633/1‑150 (MQ=255)
             gcCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAg                                                                                                                                         <  2:287978/150‑1 (MQ=255)
             gcCCAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAg                                                                                                                                         >  1:2469587/1‑150 (MQ=255)
              cccAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGt                                                                                                                                        >  2:3939416/1‑150 (MQ=255)
               ccAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGtt                                                                                                                                       >  1:4356002/1‑150 (MQ=255)
                ctaaaCGGGCATCAGCTATGAGAGTAGTTTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGACATTTAAGAAAATAGATTTTCCCGGACAGTTCAAAGCGTAATTTACGACGAGTCTCCAGTTCACGAAAGAACTCGCCGTTCAGttt                                                                                                                                      <  2:1184005/148‑1 (MQ=255)
                cAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGttt                                                                                                                                      <  2:1861293/150‑1 (MQ=255)
                cAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGttt                                                                                                                                      <  2:3337635/150‑1 (MQ=255)
                cAAAACGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGttt                                                                                                                                      <  1:1109776/150‑1 (MQ=255)
                 aaaaCGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGtttt                                                                                                                                     <  2:2140574/150‑1 (MQ=255)
                 aaaaCGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGtttt                                                                                                                                     <  2:1642740/150‑1 (MQ=255)
                 aaaaCGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGtttt                                                                                                                                     <  1:3622131/150‑1 (MQ=255)
                  aaaCGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGttttt                                                                                                                                    >  2:2461912/1‑150 (MQ=255)
                   aaCGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGtttttt                                                                                                                                   <  2:4442055/150‑1 (MQ=255)
                     cGGGCATCAGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGa                                                                                                                                 <  2:1872589/150‑1 (MQ=255)
                      gggCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTTTTTTGAc                                                                                                                                >  1:2208969/1‑150 (MQ=255)
                                 aTGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTATGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAg                                                                                                                     <  1:1677086/150‑1 (MQ=255)
                                 aTGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAg                                                                                                                     >  1:1857766/1‑150 (MQ=255)
                                  tGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGc                                                                                                                    <  2:3670553/150‑1 (MQ=255)
                                  tGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGc                                                                                                                    <  2:877405/150‑1 (MQ=255)
                                  tGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGc                                                                                                                    >  1:2027724/1‑150 (MQ=255)
                                    agagTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCAt                                                                                                                  >  2:62085/1‑150 (MQ=255)
                                    agagTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTAAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTAAGTTTTTTGACCGATGCAAAATCaa                                                                                                                  >  1:139311/1‑149 (MQ=255)
                                      agTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCt                                                                                                                >  1:3526065/1‑150 (MQ=255)
                                            ttttttATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCa                                                                                                          <  2:4341414/150‑1 (MQ=255)
                                                 tataATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCa                                                                                                     >  2:872494/1‑150 (MQ=255)
                                                   taATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCaaa                                                                                                   >  1:354996/1‑150 (MQ=255)
                                                    aaTCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAg                                                                                                  >  1:4203199/1‑150 (MQ=255)
                                                     aTCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAgt                                                                                                 <  1:1154662/150‑1 (MQ=255)
                                                       ctctTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAgtgt                                                                                               <  1:3867979/150‑1 (MQ=255)
                                                        tctTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGtgtg                                                                                              >  2:1152406/1‑150 (MQ=255)
                                                        tctTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGtgtg                                                                                              >  2:1303307/1‑150 (MQ=255)
                                                        tctTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGGTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGtgtg                                                                                              >  2:3491400/1‑150 (MQ=255)
                                                           tttAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCa                                                                                           <  2:1457724/150‑1 (MQ=255)
                                                                    gtcCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGctct                                                                                  <  2:708910/150‑1 (MQ=255)
                                                                      ccccTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCttt                                                                                >  2:3390543/1‑150 (MQ=255)
                                                                       cccTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCCAAGTGTGGCAAGCGGCTCtttt                                                                               >  2:2725078/1‑150 (MQ=255)
                                                                       cccTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCtttt                                                                               >  2:2756207/1‑150 (MQ=255)
                                                                       cccTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCtttt                                                                               <  1:505754/150‑1 (MQ=255)
                                                                        ccTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTa                                                                              >  2:458765/1‑150 (MQ=255)
                                                                          ttGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAAt                                                                            <  2:1367845/150‑1 (MQ=255)
                                                                             tgCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCa                                                                         >  1:3787646/1‑150 (MQ=255)
                                                                                    tAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCt                                                                  <  2:1088772/150‑1 (MQ=255)
                                                                                     aaGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTa                                                                 >  2:2235545/1‑150 (MQ=255)
                                                                                     aaGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTa                                                                 <  1:788911/150‑1 (MQ=255)
                                                                                     aaGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTa                                                                 <  1:267217/150‑1 (MQ=255)
                                                                                        aaaaTAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAac                                                              <  2:692681/150‑1 (MQ=255)
                                                                                         aaaTAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAacc                                                             >  2:1579567/1‑150 (MQ=255)
                                                                                         aaaTAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAacc                                                             >  1:3451187/1‑150 (MQ=255)
                                                                                           aTAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAaccac                                                           >  2:2979637/1‑150 (MQ=255)
                                                                                              gCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTg                                                        <  2:547101/150‑1 (MQ=255)
                                                                                              gCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTg                                                        <  2:546994/150‑1 (MQ=255)
                                                                                               cTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTAACACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCCCCTGt                                                       >  1:1243776/1‑150 (MQ=255)
                                                                                                ttttCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTa                                                      <  1:4536204/150‑1 (MQ=255)
                                                                                                 tttCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTaa                                                     >  1:1729900/1‑150 (MQ=255)
                                                                                                 tttCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTaa                                                     >  1:3818925/1‑150 (MQ=255)
                                                                                                 tttCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTaa                                                     >  1:4312499/1‑150 (MQ=255)
                                                                                                  ttCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAAt                                                    <  2:1808321/150‑1 (MQ=255)
                                                                                                  ttCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAAt                                                    >  1:2775712/1‑150 (MQ=255)
                                                                                                   tCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATg                                                   >  1:1653538/1‑150 (MQ=255)
                                                                                                   tCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATg                                                   >  1:4125985/1‑150 (MQ=255)
                                                                                                     ccGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGat                                                 <  2:1993054/150‑1 (MQ=255)
                                                                                                     ccGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGat                                                 <  1:850298/150‑1 (MQ=255)
                                                                                                     ccGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGGTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGat                                                 <  2:3970791/150‑1 (MQ=255)
                                                                                                      cGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGata                                                >  2:1218190/1‑150 (MQ=255)
                                                                                                           aGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCGGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCcgc                                           <  2:1149946/150‑1 (MQ=255)
                                                                                                           aGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCcgc                                           >  1:4232032/1‑150 (MQ=255)
                                                                                                                aaaGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCtt                                      >  2:836658/1‑150 (MQ=255)
                                                                                                                 aaGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCAGCAAAAGCATCATTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCttt                                     >  2:2114330/1‑150 (MQ=255)
                                                                                                                      tAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTg                                <  1:3657498/150‑1 (MQ=255)
                                                                                                                      tAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTg                                >  2:3752193/1‑150 (MQ=255)
                                                                                                                        aTGTACTACGAGTCTCCAGTTCACGAAAGACCTCGCCGTACAGTTTTTAGACATCTTCAAAAGCATCTTTACAACGCCAAAGTGTGGCAAGCGGCTCTTATAATTAAACCAACTAAACCACCTGTAAAGATATCCGCGGCTTTTTCTgga                              >  2:2971241/1‑149 (MQ=255)
                                                                                                                             cgacgaGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCt                         <  2:4038682/150‑1 (MQ=255)
                                                                                                                              gacgaGTCTCCAGTTCACGAAATACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCta                        <  2:4053168/150‑1 (MQ=255)
                                                                                                                              gacgaGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTTAACTCTGCAAAATCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTAAACCATCTAAAACACCTGTAATGATATACGCGGCTTTTTATGGCAAGCta                        >  1:404343/1‑150 (MQ=255)
                                                                                                                              gacgaGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCta                        <  1:393228/150‑1 (MQ=255)
                                                                                                                               acgaGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCtat                       >  2:3900621/1‑150 (MQ=255)
                                                                                                                               acgaGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCtat                       >  1:2646513/1‑150 (MQ=255)
                                                                                                                                  aGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACa                    >  1:2913478/1‑150 (MQ=255)
                                                                                                                                  aGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTGTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACa                    <  2:3087330/150‑1 (MQ=255)
                                                                                                                                   gTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACaa                   <  1:3315018/150‑1 (MQ=255)
                                                                                                                                     ctcCAGTTCACGAAAGACCTCGCCGTTCATTTTTTTGACCTCTGCAAAAGCATATTTACCACGCCCAAGTGTGGCAAGAGGCTCTTTTAATTCAACCATCTAAACAACCTGTAATGTTATCCGCGGCTTTTTCTGGCAAGCTATACAAAt                 >  2:20880/1‑150 (MQ=255)
                                                                                                                                     ctcCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAAt                 >  1:4600542/1‑150 (MQ=255)
                                                                                                                                       ccAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATcc               <  2:4215895/150‑1 (MQ=255)
                                                                                                                                             cACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTa         >  2:1662357/1‑150 (MQ=255)
                                                                                                                                              aCGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTAt        <  2:1064000/150‑1 (MQ=255)
                                                                                                                                                gAAATACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCAGAGTATCg      >  1:1115610/1‑150 (MQ=255)
                                                                                                                                                gAAATACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCATTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTAAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTATGGCAAGCTATACAAATCCTGAGTATCg      >  1:2404423/1‑150 (MQ=255)
                                                                                                                                                gAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCg      >  2:4418549/1‑150 (MQ=255)
                                                                                                                                                  aaGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGc    <  2:1526890/150‑1 (MQ=255)
                                                                                                                                                    gACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCAt  >  2:4513287/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCAT  >  CP009273/3799079‑3799376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑