Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,834,958 G→T 20.7% Q48K (CAG→AAG)  nepI ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,834,9580GT20.7% 744.7 / 134.0 272Q48K (CAG→AAG) nepIputative transporter
Reads supporting (aligned to +/- strand):  ref base G (137/78);  new base T (30/27);  total (167/105)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

GAGCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTAC  >  CP009273/3834809‑3835107
                                                                                                                                                     |                                                                                                                                                     
gagCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTg                                                                                                                                                       >  1:202890/1‑150 (MQ=255)
gagCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTg                                                                                                                                                       >  1:2263824/1‑150 (MQ=255)
 agCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTgg                                                                                                                                                      <  2:697114/150‑1 (MQ=255)
   cGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGc                                                                                                                                                    <  1:585914/150‑1 (MQ=255)
    gTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGcc                                                                                                                                                   >  2:3456478/1‑150 (MQ=255)
     tCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCa                                                                                                                                                  <  1:4251576/150‑1 (MQ=255)
      cAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTTGGCCAt                                                                                                                                                 <  1:807969/150‑1 (MQ=255)
        gCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTg                                                                                                                                               <  1:2374087/150‑1 (MQ=255)
        gCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTg                                                                                                                                               >  2:3803034/1‑150 (MQ=255)
           aaaCGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCg                                                                                                                                            >  1:3943097/1‑150 (MQ=255)
           aaaCGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCg                                                                                                                                            <  2:3125510/150‑1 (MQ=255)
           aaaCGGCAAACAAAATAACAACGGAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTTGGCCATTGGCg                                                                                                                                            <  2:1291590/150‑1 (MQ=255)
             aCGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTc                                                                                                                                          <  2:4436893/150‑1 (MQ=255)
              cGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTca                                                                                                                                         >  2:3705813/1‑150 (MQ=255)
                gCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTcaac                                                                                                                                       >  2:3050357/1‑150 (MQ=255)
                gCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTcaac                                                                                                                                       >  2:253409/1‑150 (MQ=255)
                   aacaaaATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACaaa                                                                                                                                    >  2:937993/1‑150 (MQ=255)
                       aaaTAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGa                                                                                                                                >  1:4635080/1‑150 (MQ=255)
                        aaTAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGAc                                                                                                                               <  1:3026480/150‑1 (MQ=255)
                         aTAACAACGTAGCGGCGGTCAGTAGCCTGAAATGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCCTTGGCGTCAACAAACTGACg                                                                                                                              >  1:773137/1‑150 (MQ=255)
                           aacaacGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACggg                                                                                                                            >  1:1819660/1‑150 (MQ=255)
                           aacaacGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATAGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACggg                                                                                                                            >  1:1126081/1‑150 (MQ=255)
                             caacGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCa                                                                                                                          >  1:1576960/1‑150 (MQ=255)
                               acGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCaaa                                                                                                                        <  1:3462235/150‑1 (MQ=255)
                                cGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCaaaa                                                                                                                       >  1:1244886/1‑150 (MQ=255)
                                  tAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAAc                                                                                                                     <  1:3017165/150‑1 (MQ=255)
                                     cggcggTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCa                                                                                                                  >  2:542120/1‑150 (MQ=255)
                                        cggTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAATATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACg                                                                                                               >  1:4595589/1‑150 (MQ=255)
                                        cggTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACg                                                                                                               >  1:2616363/1‑150 (MQ=255)
                                         ggTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGa                                                                                                              >  1:1445205/1‑150 (MQ=255)
                                          gTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGAt                                                                                                             >  1:1379241/1‑150 (MQ=255)
                                           tCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATa                                                                                                            >  1:2237955/1‑150 (MQ=255)
                                            cAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATaa                                                                                                           >  1:725534/1‑150 (MQ=255)
                                             aGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAAt                                                                                                          >  1:2160816/1‑150 (MQ=255)
                                                 gCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGa                                                                                                      >  2:3018638/1‑150 (MQ=255)
                                                  ccTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAAAGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGAc                                                                                                     >  2:3465905/1‑150 (MQ=255)
                                                   cTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACa                                                                                                    >  1:1707963/1‑150 (MQ=255)
                                                     gAATTGTCTGGGTAATAAACAAACTGGCAAACATTGACACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAgg                                                                                                  >  2:3984785/1‑150 (MQ=255)
                                                        ttGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGa                                                                                               <  2:3191473/150‑1 (MQ=255)
                                                         tGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGac                                                                                              >  1:3244946/1‑150 (MQ=255)
                                                          gTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGaca                                                                                             >  1:4135330/1‑150 (MQ=255)
                                                            cTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGAcaca                                                                                           <  1:1677675/150‑1 (MQ=255)
                                                            cTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGAcaca                                                                                           >  1:3775882/1‑150 (MQ=255)
                                                             tGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCGGACAGGCGACACaa                                                                                          >  2:3869835/1‑150 (MQ=255)
                                                              gggTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGGCAGGCGACACaaa                                                                                         >  1:4620987/1‑150 (MQ=255)
                                                                  aataaACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGc                                                                                     >  2:1266212/1‑150 (MQ=255)
                                                                   ataaACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGcc                                                                                    >  2:3230397/1‑150 (MQ=255)
                                                                    taaACAAACTGGCAAACATTGCCACCTCGGCGGTCACGGTCACCGCTTGCCCGGCATCCCCTTCCGCAAGGCCTAAATCCTGGGCAATTGGCGTCAACAATCGGACGGGCAACAACTCAACGATAATCAGACAGGCGACACAAACCGCCa                                                                                   <  2:2050571/150‑1 (MQ=255)
                                                                    taaACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCa                                                                                   <  2:3549128/150‑1 (MQ=255)
                                                                    taaACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCa                                                                                   >  1:2474892/1‑150 (MQ=255)
                                                                      aacaaacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCAcc                                                                                 >  2:4492395/1‑150 (MQ=255)
                                                                      aacaaacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCAcc                                                                                 >  2:2312076/1‑150 (MQ=255)
                                                                      aacaaacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCAcc                                                                                 <  2:746382/150‑1 (MQ=255)
                                                                      aacaaacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCAcc                                                                                 >  2:1879/1‑150 (MQ=255)
                                                                        caaacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCga                                                                               >  2:170013/1‑150 (MQ=255)
                                                                         aaacTGGCAAACATTGCCACAAAGGCGGACACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCgag                                                                              >  1:1483388/1‑150 (MQ=255)
                                                                            cTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGaaa                                                                           <  1:1355615/150‑1 (MQ=255)
                                                                             tGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGaaaa                                                                          <  1:2997189/150‑1 (MQ=255)
                                                                              ggCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAAc                                                                         <  1:910814/150‑1 (MQ=255)
                                                                                cAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACgg                                                                       <  1:700656/150‑1 (MQ=255)
                                                                                      ttGCCACAACGGCGGTCTCGGTCACCGATTGCCCGGCAACCCCTTCCGAAAGGCCTACCTCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCACCGATACTCAGACAGGCGACACAAAACGCCACCGAGACAACGGCTGAcc                                                                 <  2:2189490/150‑1 (MQ=255)
                                                                                       tGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCa                                                                >  1:3289722/1‑150 (MQ=255)
                                                                                        gCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAg                                                               <  2:2794907/150‑1 (MQ=255)
                                                                                         ccACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGt                                                              <  1:1983729/150‑1 (MQ=255)
                                                                                         acacaAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGt                                                              >  1:367140/2‑150 (MQ=255)
                                                                                          cacaAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGtt                                                             >  2:1247132/1‑150 (MQ=255)
                                                                                            caAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcg                                                           >  2:3164595/1‑150 (MQ=255)
                                                                                            caAAGGCGATCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcg                                                           >  1:2784765/1‑150 (MQ=255)
                                                                                                ggCGGTCACGGTCCCCGATTGCCCGGCAACCCCTTCCGAACTGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcggtcg                                                       <  1:387778/150‑1 (MQ=255)
                                                                                                ggCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcggtcg                                                       >  2:1090697/1‑150 (MQ=255)
                                                                                                     tcacggtcacCGATTGCCCGGCAACCCCTTCCGAACTGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGa                                                  <  1:1425722/150‑1 (MQ=255)
                                                                                                      cacggtcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTgtt                                                 >  2:1655023/1‑148 (MQ=255)
                                                                                                       acggtcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAACCGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATg                                                <  2:3433474/150‑1 (MQ=255)
                                                                                                       acggtcacCGATTGCCCGGCAACCCCGTCCGCAATGCCTAAATCCTGGGCCATTGGCGTCAACCAACGGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATg                                                <  2:2365230/150‑1 (MQ=255)
                                                                                                        cggtcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAAAGGCTGACCAGTTCGGTCGGGTGATgg                                               >  2:338776/1‑150 (MQ=255)
                                                                                                         ggtcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGc                                              >  2:4123188/1‑150 (MQ=255)
                                                                                                            cacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATc                                           >  1:4404360/1‑150 (MQ=255)
                                                                                                             acCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATcc                                          <  1:2902260/150‑1 (MQ=255)
                                                                                                                gATTGCCCGGCAACCCATTCCGAAATGCCTAAATCCTGGGCCATTTGCGTCAACAAACTTAAGGGCAAAAACTAAACTATAATCAGACAGGCGACACAAAACGCCACAGAGAAAACTGCTGACAAGTACGGTCGGCTGATGTCATccgcg                                       >  2:482492/1‑150 (MQ=255)
                                                                                                                      ccGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCgg                                 >  1:208814/1‑150 (MQ=255)
                                                                                                                       cGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGt                                >  1:3453573/1‑150 (MQ=255)
                                                                                                                        ggCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGtt                               <  1:3340677/150‑1 (MQ=255)
                                                                                                                             ccccTTCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCgg                          <  1:1908084/150‑1 (MQ=255)
                                                                                                                             ccccTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCgg                          >  1:3063706/1‑150 (MQ=255)
                                                                                                                              cccTTCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGc                         >  2:1329069/1‑150 (MQ=255)
                                                                                                                                cTTCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCaa                       >  1:1014954/1‑150 (MQ=255)
                                                                                                                                  tCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATa                     <  2:21346/150‑1 (MQ=255)
                                                                                                                                    cGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATaaa                   >  2:1026248/1‑150 (MQ=255)
                                                                                                                                     gAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAAt                  >  2:1479020/1‑150 (MQ=255)
                                                                                                                                      aaaTGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAAtt                 >  1:103558/1‑150 (MQ=255)
                                                                                                                                      aaaTGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAAtt                 >  1:2128810/1‑150 (MQ=255)
                                                                                                                                             tAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCAt          <  1:2458533/150‑1 (MQ=255)
                                                                                                                                             tAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCAt          <  1:4533576/150‑1 (MQ=255)
                                                                                                                                              aaaTCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATg         >  1:5088/1‑150 (MQ=255)
                                                                                                                                              aaaTCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATg         >  1:2444628/1‑150 (MQ=255)
                                                                                                                                                 tCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGgtg      <  2:4204160/150‑1 (MQ=255)
                                                                                                                                                   cTGGGCCTTTGGCGTAAACAAACTGACGGTCAAAAACTCAACGATACTCAGACAGGCGACACTCAACGCCACCGAGAAAACGGCTGACCTGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGtt    <  2:2951917/150‑1 (MQ=255)
                                                                                                                                                   cTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGtt    <  2:3513283/150‑1 (MQ=255)
                                                                                                                                                     gggCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTAc  >  2:1393086/1‑150 (MQ=255)
                                                                                                                                                     gggCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTAc  >  2:1384168/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAGCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTAC  >  CP009273/3834809‑3835107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑