BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,860,653	C→A	92.6%	E44* (GAA→TAA)	ibpA ←	heat shock chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,860,653	0	C	A	92.6%	838.6 / 58.6	283	E44* (GAA→TAA)	ibpA	heat shock chaperone
Reads supporting (aligned to +/- strand): ref base C (9/12); new base A (131/131); total (140/143)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 283 total aligned reads displayed.

GTTCAGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTG  >  CP009273/3860506‑3860797

gTTCAGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGt                                                                                                                                                >  1:4495105/1‑150 (MQ=255)

gTTCAGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGt                                                                                                                                                >  1:3449771/1‑150 (MQ=255)

gTTCAGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGt                                                                                                                                                >  2:3528292/1‑150 (MQ=255)

  tCAGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCt                                                                                                                                              <  1:117133/150‑1 (MQ=255)

    aGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTAc                                                                                                                                            <  2:2472735/150‑1 (MQ=255)

    aGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTAc                                                                                                                                            >  2:3650194/1‑150 (MQ=255)

      cGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCa                                                                                                                                          >  1:896770/1‑150 (MQ=255)

        aTGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGt                                                                                                                                        <  2:1950996/150‑1 (MQ=255)

        aTGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGt                                                                                                                                        >  1:2866854/1‑150 (MQ=255)

        aTGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGt                                                                                                                                        <  2:1261165/150‑1 (MQ=255)

        aTGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGt                                                                                                                                        <  2:44506/150‑1 (MQ=255)

          gCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTc                                                                                                                                      <  1:2204193/150‑1 (MQ=255)

          gCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTc                                                                                                                                      <  2:1788579/150‑1 (MQ=255)

            ccTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCaa                                                                                                                                    >  2:2213670/1‑150 (MQ=255)

                gTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGtt                                                                                                                                >  1:3488511/1‑150 (MQ=255)

                gTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGtt                                                                                                                                >  1:1299202/1‑150 (MQ=255)

                   cAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTtata                                                                                                                             >  1:2724074/1‑150 (MQ=255)

                    aGATAGTTGCGCTCTTATAGTTCGTCGGCTTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTaataa                                                                                                                            >  2:3822818/1‑149 (MQ=255)

                    aGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATAc                                                                                                                            >  2:2460189/1‑150 (MQ=255)

                         ggTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAgg                                                                                                                       <  2:2575948/150‑1 (MQ=255)

                         ggTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAgg                                                                                                                       <  2:3149948/150‑1 (MQ=255)

                            gcgcTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTa                                                                                                                    <  2:4548312/150‑1 (MQ=255)

                             cgcTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAg                                                                                                                   <  1:3523237/150‑1 (MQ=255)

                             cgcTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAg                                                                                                                   >  2:4122592/1‑150 (MQ=255)

                              gcTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAgc                                                                                                                  >  1:2426150/1‑150 (MQ=255)

                                  tttttGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAgccgcc                                                                                                              <  2:1416524/150‑1 (MQ=255)

                                        ttCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTAct                                                                                                        >  2:831181/1‑150 (MQ=255)

                                         tcgtcgGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActc                                                                                                       >  2:389235/1‑150 (MQ=255)

                                           gtcgGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActctg                                                                                                     <  2:2830001/150‑1 (MQ=255)

                                           gtcgGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActctg                                                                                                     <  1:3008907/150‑1 (MQ=255)

                                              ggCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActctggct                                                                                                  <  1:2454627/150‑1 (MQ=255)

                                                cGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActctggctct                                                                                                <  1:2494468/150‑1 (MQ=255)

                                                cGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActctggctct                                                                                                <  2:66712/150‑1 (MQ=255)

                                                 gTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActctggctctg                                                                                               >  1:311266/1‑150 (MQ=255)

                                                 gTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTActctggctctg                                                                                               <  2:3331521/150‑1 (MQ=255)

                                                   gAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGgt                                                                                             >  1:969555/1‑150 (MQ=255)

                                                   gAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGgt                                                                                             >  2:2623111/1‑150 (MQ=255)

                                                   gAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGgt                                                                                             >  1:3238167/1‑150 (MQ=255)

                                                     gCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGgttg                                                                                           >  1:1709434/1‑150 (MQ=255)

                                                     gCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGgttg                                                                                           >  2:1648052/1‑150 (MQ=255)

                                                        ccTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGATCTCAGCAAACCCAGCCACTGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATAAGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGttt                                                                                        <  2:2386737/150‑1 (MQ=255)

                                                            tCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTa                                                                                    <  2:3917565/150‑1 (MQ=255)

                                                            tCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTa                                                                                    <  2:4014941/150‑1 (MQ=255)

                                                              accaccAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAg                                                                                  <  1:1384874/150‑1 (MQ=255)

                                                                caccaGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTg                                                                                <  1:3897653/150‑1 (MQ=255)

                                                                caccaGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTg                                                                                <  2:3459987/150‑1 (MQ=255)

                                                                caccaGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTg                                                                                <  1:252303/150‑1 (MQ=255)

                                                                caccaGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTg                                                                                >  2:308186/1‑150 (MQ=255)

                                                                caccaGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTg                                                                                <  2:12962/150‑1 (MQ=255)

                                                                 accaGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCAACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTATAAGTgg                                                                               >  2:1383320/1‑150 (MQ=255)

                                                                   cagcagATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGtt                                                                             <  1:2643165/150‑1 (MQ=255)

                                                                    agcagATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTa                                                                            >  2:371789/1‑150 (MQ=255)

                                                                        gATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTaaaca                                                                        <  2:4496127/150‑1 (MQ=255)

                                                                        gATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTaaaca                                                                        <  2:3396612/150‑1 (MQ=255)

                                                                              ccTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGt                                                                  <  2:1067517/150‑1 (MQ=255)

                                                                              ccTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGt                                                                  <  1:642221/150‑1 (MQ=255)

                                                                               cTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTc                                                                 >  1:2560108/1‑150 (MQ=255)

                                                                                ttgtcGGTAATTTAGAGTTCGCTCTCAGCAAAACCAGCCACAGAGATTGCATTGCGGTAATGTTTTTAGTCTACCATTTCAACGTTATAAGGAGGGTAGCCGCCATTACTCTGGCTCTGTTTGTTTTATATGTGGTTAAACAAACGTTCa                                                                <  2:4279313/146‑1 (MQ=255)

                                                                                tGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCa                                                                <  2:305630/150‑1 (MQ=255)

                                                                                     ggTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATcc                                                           <  1:3614716/150‑1 (MQ=255)

                                                                                       tAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTACTGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCaa                                                         >  2:224926/1‑150 (MQ=255)

                                                                                        aaTTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAAt                                                        <  1:550643/150‑1 (MQ=255)

                                                                                         aTTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATa                                                       <  2:4155613/150‑1 (MQ=255)

                                                                                         aTTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATa                                                       <  1:1972029/150‑1 (MQ=255)

                                                                                         aTTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATa                                                       <  1:3069365/150‑1 (MQ=255)

                                                                                         aTTTCCAGTTCGCTCTAAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATa                                                       >  2:4477360/1‑150 (MQ=255)

                                                                                          tttCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAg                                                      >  1:1092282/1‑150 (MQ=255)

                                                                                            tCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCa                                                    <  1:1206727/150‑1 (MQ=255)

                                                                                               aGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGaa                                                 >  2:1134938/1‑150 (MQ=255)

                                                                                                gTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAAc                                                <  2:3889463/150‑1 (MQ=255)

                                                                                                  gcGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTCAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACgg                                              >  1:3817511/2‑150 (MQ=255)

                                                                                                      tctcAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTaaa                                          <  1:238693/150‑1 (MQ=255)

                                                                                                      tctcAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTTGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTaaa                                          <  2:4222763/150‑1 (MQ=255)

                                                                                                      tctcAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTaaa                                          >  1:1857589/1‑150 (MQ=255)

                                                                                                      tctaAGCAAAAACAGCCTCAGCGATAGCAATTCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCTCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACTTACGTTCAAATCCATTAGCAGAACGGTaaa                                          <  1:643149/150‑1 (MQ=255)

                                                                                                        tcAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGc                                        >  2:1911843/1‑150 (MQ=255)

                                                                                                        tcAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGc                                        <  1:1583594/150‑1 (MQ=255)

                                                                                                        tcAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGc                                        >  1:4077783/1‑150 (MQ=255)

                                                                                                          aGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCgg                                      <  2:576695/150‑1 (MQ=255)

                                                                                                              aaaCCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGAt                                  <  2:1993173/150‑1 (MQ=255)

                                                                                                                  cAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAAt                              <  1:4001531/150‑1 (MQ=255)

                                                                                                                    gCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCa                            <  2:1504419/150‑1 (MQ=255)

                                                                                                                    gCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCa                            >  1:1085242/1‑150 (MQ=255)

                                                                                                                    gCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCa                            <  2:510062/150‑1 (MQ=255)

                                                                                                                    gCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCAAATAGCAGAACGGTAAATAGGGGAAAAATCa                            >  2:377288/1‑150 (MQ=255)

                                                                                                                     ccACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCaa                           >  1:2756719/1‑150 (MQ=255)

                                                                                                                         aGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGtt                       >  2:2769387/1‑150 (MQ=255)

                                                                                                                         aGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGtt                       >  1:2262372/1‑150 (MQ=255)

                                                                                                                         aGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGtt                       >  1:787114/1‑150 (MQ=255)

                                                                                                                          gCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTa                      <  2:2950421/150‑1 (MQ=255)

                                                                                                                            gATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACg                    <  1:4389419/150‑1 (MQ=255)

                                                                                                                               aGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCAt                 >  1:3756769/1‑150 (MQ=255)

                                                                                                                               aGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCAt                 <  1:731429/150‑1 (MQ=255)

                                                                                                                                  aaTGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAAt              <  1:595067/150‑1 (MQ=255)

                                                                                                                                   aTGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATc             <  2:911551/150‑1 (MQ=255)

                                                                                                                                         tAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGc       <  2:3484353/150‑1 (MQ=255)

                                                                                                                                           aTGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTc     <  2:4247167/150‑1 (MQ=255)

                                                                                                                                             ggTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCt   <  1:1380856/150‑1 (MQ=255)

                                                                                                                                              gTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTg  >  1:836929/1‑150 (MQ=255)

                                                                                                                                              gTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTg  >  1:3382655/1‑150 (MQ=255)

GTTCAGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTG  >  CP009273/3860506‑3860797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`