Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,891,421 C→A 8.7% H14N (CAT→AAT)  cbrC → UPF0167 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,891,4210CA 8.7% 917.6 / 39.8 245H14N (CAT→AAT) cbrCUPF0167 family protein
Reads supporting (aligned to +/- strand):  ref base C (132/91);  new base A (12/10);  total (144/101)
Fisher's exact test for biased strand distribution p-value = 8.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

AATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGAC  >  CP009273/3891273‑3891567
                                                                                                                                                    |                                                                                                                                                  
    aTTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATccc                                                                                                                                               <  2:3636044/150‑1 (MQ=255)
            tCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACt                                                                                                                                       >  2:305247/1‑150 (MQ=255)
            tCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACt                                                                                                                                       >  1:4537528/1‑150 (MQ=255)
             cTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTg                                                                                                                                      <  1:2037546/150‑1 (MQ=255)
              tACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTgg                                                                                                                                     >  1:1569712/1‑150 (MQ=255)
                  cGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAAc                                                                                                                                 <  1:1274165/150‑1 (MQ=255)
                   ggATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACa                                                                                                                                >  2:4057484/1‑150 (MQ=255)
                      ttGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGgc                                                                                                                             >  1:2659911/1‑150 (MQ=255)
                      ttGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGgc                                                                                                                             <  1:2420310/150‑1 (MQ=255)
                       tGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGgcg                                                                                                                            >  1:1750218/1‑150 (MQ=255)
                        ggATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGgcgc                                                                                                                           >  2:2869171/1‑150 (MQ=255)
                          atatTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCAt                                                                                                                         >  2:3792958/1‑150 (MQ=255)
                             tttCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTg                                                                                                                      <  1:4227084/150‑1 (MQ=255)
                             tttCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTg                                                                                                                      <  2:270188/150‑1 (MQ=255)
                                cATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAAc                                                                                                                   >  2:1711956/1‑150 (MQ=255)
                                 aTGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACa                                                                                                                  <  2:3001901/150‑1 (MQ=255)
                                  tGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAg                                                                                                                 <  1:3358039/150‑1 (MQ=255)
                                     ccTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGAt                                                                                                              >  2:3895720/1‑150 (MQ=255)
                                      cTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATa                                                                                                             <  1:561455/150‑1 (MQ=255)
                                       tGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATaa                                                                                                            >  2:978696/1‑150 (MQ=255)
                                       tGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATaa                                                                                                            <  2:3115334/150‑1 (MQ=255)
                                         aaaCAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATaaaa                                                                                                          >  2:1637384/1‑150 (MQ=255)
                                          aaCAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAAc                                                                                                         <  1:1907148/150‑1 (MQ=255)
                                           aCAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAAcc                                                                                                        >  2:3297284/1‑150 (MQ=255)
                                             aGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGt                                                                                                      >  1:1307980/1‑150 (MQ=255)
                                              gACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTa                                                                                                     >  2:544504/1‑150 (MQ=255)
                                              gACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTa                                                                                                     <  2:3834673/150‑1 (MQ=255)
                                              gACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTa                                                                                                     >  1:3909897/1‑150 (MQ=255)
                                                 tctTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTagag                                                                                                  >  1:4099128/1‑150 (MQ=255)
                                                 tctTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTagag                                                                                                  >  2:2153099/1‑150 (MQ=255)
                                                  ctTTAAGTTAATCGGGATACTTTATCTTTGGTCTACTCAAAAGCAGACAGGAAGTTTCTATGACTAAAAAAATCAGGCCGTTACCCCAAATCAAATATCATACCAATCCACAGGAAACAGGCGCATATGAACAGGATAAAACCGTATAGt                                                                                                 >  1:1934293/1‑150 (MQ=255)
                                                  ctTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGt                                                                                                 <  1:426933/150‑1 (MQ=255)
                                                  ctTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGt                                                                                                 >  1:413319/1‑150 (MQ=255)
                                                   tttAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTg                                                                                                >  2:1323613/1‑150 (MQ=255)
                                                   tttAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTg                                                                                                >  2:1925393/1‑150 (MQ=255)
                                                     taagttaagCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCg                                                                                              >  2:3128707/1‑150 (MQ=255)
                                                         ttaagCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTg                                                                                          <  2:1479029/150‑1 (MQ=255)
                                                               gggATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGa                                                                                    >  1:2458281/1‑150 (MQ=255)
                                                               gggATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGa                                                                                    >  1:2295789/1‑150 (MQ=255)
                                                                ggATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGaa                                                                                   >  2:824442/1‑150 (MQ=255)
                                                                ggATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGaa                                                                                   >  2:2212204/1‑150 (MQ=255)
                                                                  aTACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGaaca                                                                                 <  2:186073/150‑1 (MQ=255)
                                                                     cTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAacaaca                                                                              <  1:562479/150‑1 (MQ=255)
                                                                        tATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGAc                                                                           >  2:3853928/1‑150 (MQ=255)
                                                                         aTCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACg                                                                          <  1:2059176/150‑1 (MQ=255)
                                                                           cTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTc                                                                        <  1:2676934/150‑1 (MQ=255)
                                                                           cTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTc                                                                        <  1:2928488/150‑1 (MQ=255)
                                                                              tGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGt                                                                     >  2:2296665/1‑150 (MQ=255)
                                                                               gggCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGtt                                                                    <  2:357987/150‑1 (MQ=255)
                                                                                ggCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGttt                                                                   <  1:610119/150‑1 (MQ=255)
                                                                                 gCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTtta                                                                  <  2:1091027/150‑1 (MQ=255)
                                                                                   tACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTttatt                                                                <  1:1059301/150‑1 (MQ=255)
                                                                                   tACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTttatt                                                                >  2:4388600/1‑150 (MQ=255)
                                                                                   tACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTttatt                                                                >  2:3328712/1‑150 (MQ=255)
                                                                                        aaaaGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCg                                                           >  2:455898/1‑150 (MQ=255)
                                                                                        aaaaGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCg                                                           >  1:2126672/1‑150 (MQ=255)
                                                                                        aaaaGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCg                                                           >  1:3592763/1‑150 (MQ=255)
                                                                                         aaaGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCgg                                                          >  1:3877438/1‑150 (MQ=255)
                                                                                          aaGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCggg                                                         >  2:344866/1‑150 (MQ=255)
                                                                                          aaGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCggg                                                         <  2:2578105/150‑1 (MQ=255)
                                                                                           aGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGt                                                        <  2:3538998/150‑1 (MQ=255)
                                                                                           aGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGt                                                        >  1:714015/1‑150 (MQ=255)
                                                                                           aGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGt                                                        >  1:714083/1‑150 (MQ=255)
                                                                                           aGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGt                                                        <  1:3414892/150‑1 (MQ=255)
                                                                                             cAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTcc                                                      <  2:1040035/150‑1 (MQ=255)
                                                                                             cAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTcc                                                      >  2:42261/1‑150 (MQ=255)
                                                                                             cAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTcc                                                      >  1:944914/1‑150 (MQ=255)
                                                                                              aGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTccc                                                     >  1:2216437/1‑150 (MQ=255)
                                                                                               gACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCt                                                    >  2:4124122/1‑150 (MQ=255)
                                                                                                aCAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAGGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCtt                                                   <  1:3298065/150‑1 (MQ=255)
                                                                                                      tGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTg                                             >  2:3690536/1‑150 (MQ=255)
                                                                                                        tttCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCg                                           >  1:1483544/1‑150 (MQ=255)
                                                                                                          tCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGtt                                         <  1:767738/150‑1 (MQ=255)
                                                                                                          tCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGtt                                         <  2:2434373/150‑1 (MQ=255)
                                                                                                              tGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTtgatg                                     >  1:4419122/1‑150 (MQ=255)
                                                                                                               gACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGAATTTTAACAGGATAAAACAGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTtgatga                                    <  2:2081829/150‑1 (MQ=255)
                                                                                                                aCTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGaa                                   <  1:3510646/150‑1 (MQ=255)
                                                                                                                   cAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGtt                                <  1:310441/150‑1 (MQ=255)
                                                                                                                   cAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGtt                                <  1:2152076/150‑1 (MQ=255)
                                                                                                                     aaaTATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGa                              <  1:1578098/150‑1 (MQ=255)
                                                                                                                       atatCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAAc                            <  1:1766591/150‑1 (MQ=255)
                                                                                                                           cAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAtct                        >  2:4417761/1‑150 (MQ=255)
                                                                                                                            aGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAtctc                       >  2:2394075/1‑150 (MQ=255)
                                                                                                                             ggCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATctct                      <  1:4002263/150‑1 (MQ=255)
                                                                                                                             ggCCGTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATctct                      <  1:500168/150‑1 (MQ=255)
                                                                                                                                 gTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTcc                  >  1:4237071/1‑150 (MQ=255)
                                                                                                                                 gTTACCCCAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTcc                  <  2:2591482/150‑1 (MQ=255)
                                                                                                                                  ttACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTGTATTACTCGGGTCCCGTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCg                 >  2:672185/1‑150 (MQ=255)
                                                                                                                                     ccccAATTCAAATATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCgtgg              >  1:931815/1‑150 (MQ=255)
                                                                                                                                      cccAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGTTGAAGTTGAACATCTCTGTCCgtggt             >  2:4506512/1‑150 (MQ=255)
                                                                                                                                       ccAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCgtggtg            >  1:3237718/1‑150 (MQ=255)
                                                                                                                                        cAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGgtgt           >  1:4309331/1‑150 (MQ=255)
                                                                                                                                         aaTTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTa          <  1:3128877/150‑1 (MQ=255)
                                                                                                                                           ttCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTAtt        <  1:80188/150‑1 (MQ=255)
                                                                                                                                           ttCAAATATCATCCCAAGCCACTGGAAACAGGCGCAATTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTAtt        <  2:226584/150‑1 (MQ=255)
                                                                                                                                           ttCAAATATAATCCAAAGCAAATGGAAACAGGCGCATTTGAACAGGATAAAACAGTAGAGTGCGATTTCTGTGAACAACAGACGTCAGATTATTACTCGGGTACCTTATATTGCGTTGTTGAAGTTGAACATCTCTGTCAGTGGTGTAta        >  2:723597/1‑149 (MQ=255)
                                                                                                                                              aaaTATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCg     >  2:1893761/1‑150 (MQ=255)
                                                                                                                                              aaaTATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCg     >  2:3680650/1‑150 (MQ=255)
                                                                                                                                               aaTATAATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCgg    >  2:1186395/1‑150 (MQ=255)
                                                                                                                                                 tatCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGAc  >  1:1399001/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAAACAGACTCTTTAAGTTAAGCGGGATACTTTATCTTTGGGCTACTCAAAAGCAGACAGGATGTTTCTATGACTCAAAATATCAGGCCGTTACCCCAATTCAAATATCATCCCAAGCCACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGAC  >  CP009273/3891273‑3891567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑