Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,894,972 G→T 11.0% G203G (GGC→GGA)  bglH ← carbohydrate‑specific outer membrane porin, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,894,9720GT11.0% 1142.8 / 74.6 318G203G (GGC→GGA) bglHcarbohydrate‑specific outer membrane porin, cryptic
Reads supporting (aligned to +/- strand):  ref base G (148/135);  new base T (13/22);  total (161/157)
Fisher's exact test for biased strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 323 total aligned reads displayed.

CGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAA  >  CP009273/3894824‑3895121
                                                                                                                                                    |                                                                                                                                                     
 ggCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGcc                                                                                                                                                     >  1:1592034/1‑150 (MQ=255)
    aTCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCAcc                                                                                                                                                  >  2:870124/1‑150 (MQ=255)
    aTCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCAcc                                                                                                                                                  >  1:2384051/1‑150 (MQ=255)
     tCAGTACGCTGCGATTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCTCCACCg                                                                                                                                                 <  2:1888130/150‑1 (MQ=255)
      cAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCgg                                                                                                                                                >  1:868839/1‑150 (MQ=255)
             cTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCTCCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATc                                                                                                                                         <  2:2730022/150‑1 (MQ=255)
             cTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATc                                                                                                                                         <  1:4608741/150‑1 (MQ=255)
             cTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATc                                                                                                                                         <  1:296140/150‑1 (MQ=255)
              tGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCg                                                                                                                                        <  1:1378273/150‑1 (MQ=255)
                 gTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGAAGGAAACCTTTGGTGGTAACGTACATATCGGAGTACTGCATATAGTTCTCGCCACCGGCGTTATCGCCa                                                                                                                                     <  1:1187303/150‑1 (MQ=255)
                  ttttCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCaa                                                                                                                                    <  2:3108389/150‑1 (MQ=255)
                  ttttCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCaa                                                                                                                                    >  2:2096474/1‑150 (MQ=255)
                   tttCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCaaa                                                                                                                                   >  2:1347444/1‑150 (MQ=255)
                     tCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAAcc                                                                                                                                 <  1:3156794/150‑1 (MQ=255)
                     tCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAAcc                                                                                                                                 >  2:3215984/1‑150 (MQ=255)
                      ccAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCa                                                                                                                                <  2:2129865/150‑1 (MQ=255)
                       cAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAg                                                                                                                               >  1:3351210/1‑150 (MQ=255)
                        aGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCTCCACCGGCGTTATCGCCAAACCAGc                                                                                                                              <  1:3870359/150‑1 (MQ=255)
                        aGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCTCCACCGGCGTTATCGCCAAACCAGc                                                                                                                              <  1:3903279/150‑1 (MQ=255)
                        aGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTACTCGCCACCGGCGTTATCGCCAAACCAGc                                                                                                                              >  2:2225513/1‑150 (MQ=255)
                         gTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGcc                                                                                                                             >  1:690706/1‑150 (MQ=255)
                            aaGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCTCCACCGGCGTTATCGCCAAACCAGCCGGt                                                                                                                          >  1:3784729/1‑150 (MQ=255)
                                aTCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTagag                                                                                                                      >  2:63560/1‑150 (MQ=255)
                                aTCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTagag                                                                                                                      >  2:1103327/1‑150 (MQ=255)
                                  cTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCTCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTa                                                                                                                    >  2:3411457/1‑150 (MQ=255)
                                    ggATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTAct                                                                                                                  <  1:1048200/150‑1 (MQ=255)
                                      aTTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTActgc                                                                                                                <  2:1500003/150‑1 (MQ=255)
                                      aTTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTActgc                                                                                                                >  2:1451285/1‑150 (MQ=255)
                                       tttCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTActgct                                                                                                               <  1:2632797/150‑1 (MQ=255)
                                       tttCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTActgct                                                                                                               <  2:2644236/150‑1 (MQ=255)
                                        ttCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTActgctg                                                                                                              <  1:1420453/150‑1 (MQ=255)
                                         tCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGa                                                                                                             >  1:3143170/1‑150 (MQ=255)
                                          cAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGAc                                                                                                            >  2:3931547/1‑150 (MQ=255)
                                           aaTTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGAcc                                                                                                           >  1:4185882/1‑150 (MQ=255)
                                               ttCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACg                                                                                                       <  1:1873721/150‑1 (MQ=255)
                                                 cGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGtt                                                                                                     <  2:2339990/150‑1 (MQ=255)
                                                  ggCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTa                                                                                                    <  1:4556461/150‑1 (MQ=255)
                                                     gcACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTAcca                                                                                                 >  1:4274254/1‑150 (MQ=255)
                                                       aCCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTAccatc                                                                                               >  2:1583385/1‑150 (MQ=255)
                                                       aCCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTAccatc                                                                                               >  2:774437/1‑150 (MQ=255)
                                                       aCCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTAccatc                                                                                               <  2:512911/150‑1 (MQ=255)
                                                          gtgtTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTAccatccat                                                                                            >  1:40097/1‑150 (MQ=255)
                                                          gtgtTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTAccatccat                                                                                            >  1:2075406/1‑150 (MQ=255)
                                                              ttACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCatcatc                                                                                        >  1:1968170/1‑150 (MQ=255)
                                                               tACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGATCTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCAtcatca                                                                                       <  2:3351400/150‑1 (MQ=255)
                                                                acccacccAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATcac                                                                                      >  2:3229759/1‑150 (MQ=255)
                                                                 cccacccaGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATcaca                                                                                     >  1:371721/1‑150 (MQ=255)
                                                                  ccacccaGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACaa                                                                                    >  1:747900/1‑150 (MQ=255)
                                                                   cacccaGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAAc                                                                                   <  1:3531406/150‑1 (MQ=255)
                                                                    acccaGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTTACGTACATATCGGTGTACTGCATATAGTTCTCTCCACCGGCGTTATCGCCAATCCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACg                                                                                  <  2:1273426/150‑1 (MQ=255)
                                                                    acccaGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACg                                                                                  <  1:4426165/150‑1 (MQ=255)
                                                                    acccaGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACg                                                                                  <  1:357842/150‑1 (MQ=255)
                                                                     cccaGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACACCgg                                                                                 <  2:3646189/150‑1 (MQ=255)
                                                                      ccaGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGc                                                                                <  2:1014418/150‑1 (MQ=255)
                                                                       caGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCa                                                                               <  2:2410220/150‑1 (MQ=255)
                                                                        aGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCAt                                                                              <  2:378778/150‑1 (MQ=255)
                                                                         gAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATc                                                                             <  2:2843803/150‑1 (MQ=255)
                                                                          aaaTCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCa                                                                            >  1:2676899/1‑150 (MQ=255)
                                                                            aTCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAAc                                                                          >  1:486121/1‑150 (MQ=255)
                                                                             tCAGCCTCTGGCGCAAAGGGCAGGAAACCTCTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAAcc                                                                         >  1:2378090/1‑150 (MQ=255)
                                                                               aGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCg                                                                       >  1:3652072/1‑150 (MQ=255)
                                                                                  ctctGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGttt                                                                    >  2:2778362/1‑150 (MQ=255)
                                                                                       gcgcAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGt                                                               <  2:2430337/150‑1 (MQ=255)
                                                                                         gcAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGttt                                                             <  2:3348376/150‑1 (MQ=255)
                                                                                         gcAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGttt                                                             <  1:2339042/150‑1 (MQ=255)
                                                                                          cAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGtttt                                                            <  2:45788/150‑1 (MQ=255)
                                                                                          cAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGtttt                                                            <  1:2489652/150‑1 (MQ=255)
                                                                                          cAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGtttt                                                            >  2:2713174/1‑150 (MQ=255)
                                                                                          cAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGtttt                                                            >  2:4448600/1‑150 (MQ=255)
                                                                                           aaaGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTc                                                           >  1:1776832/1‑150 (MQ=255)
                                                                                           aaaGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTc                                                           <  1:2201587/150‑1 (MQ=255)
                                                                                            aaGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCg                                                          >  2:1999953/1‑150 (MQ=255)
                                                                                             aGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCTCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGt                                                         >  2:4029368/1‑150 (MQ=255)
                                                                                             aGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGt                                                         <  1:3672051/150‑1 (MQ=255)
                                                                                             aGGGCAGGAAACATTTGGTGGTAACGTACATATCGGAGAACTTCATATAGTTCTCTCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCAACAACCCGTATGCCGTTTTCGt                                                         >  2:1706717/1‑150 (MQ=255)
                                                                                              gggCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGtt                                                        <  2:3111189/150‑1 (MQ=255)
                                                                                               ggCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTg                                                       >  1:1246524/1‑150 (MQ=255)
                                                                                               ggCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCATTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTg                                                       >  1:439817/1‑150 (MQ=255)
                                                                                                   ggAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCTTTTGCCGTTTTCGTTGTAGa                                                   >  1:1742374/1‑150 (MQ=255)
                                                                                                        ccTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCTCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACgt                                              >  2:1952527/1‑150 (MQ=255)
                                                                                                        ccTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACgt                                              >  2:4438501/1‑150 (MQ=255)
                                                                                                         cTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACgtt                                             <  2:3073654/150‑1 (MQ=255)
                                                                                                          tttGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACgttg                                            <  1:2796519/150‑1 (MQ=255)
                                                                                                           ttGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCTCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACgttgt                                           <  2:1307623/150‑1 (MQ=255)
                                                                                                           ttGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCTCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACgttgt                                           <  1:3389356/150‑1 (MQ=255)
                                                                                                             ggtggtAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGttt                                         >  1:4467628/1‑150 (MQ=255)
                                                                                                                ggtAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTaa                                      >  1:2842000/1‑150 (MQ=255)
                                                                                                                          atatCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCa                            <  2:3464021/150‑1 (MQ=255)
                                                                                                                          atatCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCa                            >  1:4132930/1‑150 (MQ=255)
                                                                                                                            atCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCaaa                          >  1:578816/1‑150 (MQ=255)
                                                                                                                            atCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCaaa                          >  2:860331/1‑150 (MQ=255)
                                                                                                                            atCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCaaa                          >  2:916353/1‑150 (MQ=255)
                                                                                                                              cGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAAcc                        <  1:2716852/150‑1 (MQ=255)
                                                                                                                                 agaACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGc                     >  1:1107232/1‑150 (MQ=255)
                                                                                                                                 agaACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGc                     <  2:469580/150‑1 (MQ=255)
                                                                                                                                          tataGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTAtt            <  2:45016/150‑1 (MQ=255)
                                                                                                                                          tataGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGGTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTAtt            >  1:2450231/1‑150 (MQ=255)
                                                                                                                                           ataGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTc           >  1:1658711/1‑150 (MQ=255)
                                                                                                                                              gTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGtt        >  1:656244/1‑150 (MQ=255)
                                                                                                                                              gTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGtt        >  2:978930/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTTCTCGCCACCGGCGTTATCGCCAAACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAA  >  CP009273/3894824‑3895121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑